Conserved Protein Domain Family
Sina

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pfam03145: Sina 
Seven in absentia protein family
The seven in absentia (sina) gene was first identified in Drosophila. The Drosophila Sina protein is essential for the determination of the R7 pathway in photoreceptor cell development: the loss of functional Sina results in the transformation of the R7 precursor cell to a non- neuronal cell type. The Sina protein contains an N-terminal RING finger domain pfam00097. Through this domain, Sina binds E2 ubiquitin-conjugating enzymes (UbcD1) Sina also interacts with Tramtrack (TTK88) via PHYL. Tramtrack is a transcriptional repressor that blocks photoreceptor determination, while PHYL down-regulates the activity of TTK88. In turn, the activity of PHYL requires the activation of the Sevenless receptor tyrosine kinase, a process essential for R7 determination. It is thought that thus Sina targets TTK88 for degradation, therefore promoting the R7 pathway. Murine and human homologs of Sina have also been identified. The human homolog Siah-1 also binds E2 enzymes (UbcH5) and through a series of physical interactions, targets beta-catenin for ubiquitin degradation. Siah-1 expression is enhanced by p53, itself promoted by DNA damage. Thus this pathway links DNA damage to beta-catenin degradation. Sina proteins, therefore, physically interact with a variety of proteins. The N-terminal RING finger domain that binds ubiquitin conjugating enzymes is described in pfam00097, and does not form part of the alignment for this family. The remainder C-terminal part is involved in interactions with other proteins, and is included in this alignment. In addition to the Drosophila protein and mammalian homologs, whose similarity was noted previously, this family also includes putative homologs from Caenorhabditis elegans, Arabidopsis thaliana.
Statistics
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PSSM-Id: 335239
View PSSM: pfam03145
Aligned: 42 rows
Threshold Bit Score: 165.086
Threshold Setting Gi: 242023265
Created: 22-Mar-2017
Updated: 23-May-2017
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q7QF99       119 LRNYTLE-AIVSKVQFPCRNAVK-----GCSVRLPLQLLRWHKERCGYKLIECFMGKv-wGGCGWHGCERDWLAHCLAEH 191 Anopheles gambi...
Q29HR3       254 sRSLTVE-ALCAKAHFGCSHAAG-----GCQVRMPVALLPWHEQQCIYKPMKCFMGRv-wGECKWQGREVQWKEHLEEQH 326 Drosophila pseu...
Q178N6       160 MRNYTLE-AIAAKVHFPCTHAAR-----GCTVRLPLELLWWHKDRCGYKQIECFMGKv-wEDCSWHGCEKDWIGHCVTEH 232 yellow fever mo...
XP_009392365 113 IRCLALE-KLAVSLHLPCAYHHL-----GCEEMFPYYSKLQHETRCTYRPYTCPHPG---SDCPFTSDSLALLSHLRERH 183 wild Malaysian ...
XP_320652     28 kvpnpIE-KLLHQAMCPCRYAPN-----GCTWKFVQSEMDAHLVECRFRPYRCVAASlnvIKCNWQGLQHEIEDHLAKGH 101 Anopheles gambi...
XP_008195839  54 tRNFSLE-SVCTVLQYPCSNEMR-----GCSHYMKLEEFAEHQERCDYRNYRCMFEK----YCCWQGTRDKLKKHYVDKH 123 red flour beetle
XP_002432056  91 aKNIMLA-QIAEYVKYPCPNTIG-----GCEEVYYLRDEETHLKKCGYIVHRCKI-----DNCDWIGKKDELKSHVENLH 159 human body louse
XP_012551616  97 IRSAVLE-SLANKVLFPCTNA-------GCPKHATLPLLEKHTPHCQFRIINCFMARv-yGECTWEGRAGDWMDHCFSEH 167 domestic silkworm
XP_009135318  41 INHLQIEsQVHELLDYPVCTNQItstiyQCS-----NDYTGEVNNDKENPYNCPYSG---SKCTATGDTQWLLQHLRNDH 112 field mustard
NP_187978      5 INDLQVEsRVHELLDFPVHTNQIssaiyeCP--------NDHIENPKKKPYNCPHSG---AKCDVTGDIQRLLLHLRNDH 73  thale cress
Q7QF99       192 aD-HVHEDR-----CVELQWaygAEAaQ--RApELQLVVAYHVVRAYGEAFNLYQVYDQ-DSRTVLWTVICATKESQVSG 262 Anopheles gambi...
Q29HR3       327 aE-KLFRSS-----TSDLVW-------N--MAaRRKPLTGYYVFQAHDEMFNFYEIYDK---QRILFTMTCTSNRRESKY 388 Drosophila pseu...
Q178N6       233 qD-KVYNLP-----DIVLTWnyaADS-Q--RCiALQSVIAYYVIRAYGEYFNVYQIYDQ-NSRRTIWTVICASKEAKTSH 302 yellow fever mo...
XP_009392365 184 ---KVDLQA-----GCTFNHr-yVKQ-D--PCsVDSCSWTLTLFNCFGQYFCLHFEAFLlGCEPVYMAFLRFMGEESEAR 251 wild Malaysian ...
XP_320652    102 -K-EL---------GEVFRF---RES-TslVFkEQISLGGLKLVDAFSKRFLFYFFSD--VAHKKLSFLMLYFGRREEAA 164 Anopheles gambi...
XP_008195839 124 -DnNVLIGS-----TSMCLWk------------EDQPDYTVHLLLAFDELFYVHKRLR----DDVMHWAVQYIGRPEDVI 181 red flour beetle
XP_002432056 160 qE-DIWKKEwnfagSRKFEH-------N--DTsSDEFGKLLVIEK---ELFWMLSNYD--CEKKKLYKSFQYIGPKEAAK 224 human body louse
XP_012551616 168 kQ-KV----------TDLPFitvKDK-W--DAkRTEPILNYFLLRCFEKVFNVYQIYDK-RGGRMMWTVLV---NDDNAE 229 domestic silkworm
XP_009135318 113 ---HVEMHD-----GRSFSHr-yVHH-N--PKhLHHATWMLTLLDCYGRQFCLYFEAFHlKKTPMYIAYMQFMGDEEEAT 180 field mustard
NP_187978     74 ---NVEMSD-----GRSFSHr-yVHH-D--PKhLHHATWMLTLLDCCGRKFCLYFEAFHlRKTPMYMAFMQFMGDEEEAM 141 thale cress
Q7QF99       263 RYAFELEL--YS---PvntWQLLVQRFPCH---SELDPDfLKDGHCAKLALADALRFMA---Dd----KVLHYRV 322 Anopheles gambiae st...
Q29HR3       389 NYAYEVTL--LQ---PdneALSMTQKFPVH---SEYDRDiLMDGTCVSIPLNELSRFVD---Pe----KVLHYRV 448 Drosophila pseudoobs...
Q178N6       303 RFAFELEL--YS---PidsSKLLVQRFPCH---SENDADfLKDGNCAKIPLEEAIRFMT---Kd----KILYYRI 362 yellow fever mosquito
XP_009392365 252 RYNYCLEV--GG---H---GRKLRWQGVPRs-iRTHHRMvRDSHDGLIVHRSLALYFSG---Gd---qKELKLRV 311 wild Malaysian banana
XP_320652    165 QYCYELEI--SG---RpvfSRTVKFVERCNs-dSENLAElLDEERCVSLSHRQVKNYLH---E-----GKLHMRY 225 Anopheles gambiae st...
XP_008195839 182 LYYFEIQI--FTdqfN---DRKLEFSEICHddiMENIDDiIDTGFCVSVRMPILQTYIN---Ne----GVVFYKI 244 red flour beetle
XP_002432056 225 RFNYQICLksSD---D---RRQFLFKSTMDd-dNRNGSDaFESPNCTVIDFSVLKSFSTyspE-----NKLKYLY 287 human body louse
XP_012551616 230 KFYFEIDI--FL---PnlpSKRIVYRRPCK---CEKDADfLEHTQNVYIPVENVFSMLD---Ds----EALNFIv 289 domestic silkworm
XP_009135318 181 SFSYTLEI--SG---N---GRKMTWQGVPRs-iRDSHKTvRDSQDGLIITRKLALYFSA---Dns-nsKELKLKI 242 field mustard
NP_187978    142 SFSYSLQV--GG---N---GRKLTWQGVPRs-iRDSHKTvRDSQDGLIITRKLALFFST---DnnttdKELKLKV 204 thale cress
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