Conserved Protein Domain Family
TTL

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pfam03133: TTL 
Tubulin-tyrosine ligase family
Tubulins and microtubules are subjected to several post-translational modifications of which the reversible detyrosination/tyrosination of the carboxy-terminal end of most alpha-tubulins has been extensively analysed. This modification cycle involves a specific carboxypeptidase and the activity of the tubulin-tyrosine ligase (TTL). The true physiological function of TTL has so far not been established. Tubulin-tyrosine ligase (TTL) catalyzes the ATP-dependent post-translational addition of a tyrosine to the carboxy terminal end of detyrosinated alpha-tubulin. In normally cycling cells, the tyrosinated form of tubulin predominates. However, in breast cancer cells, the detyrosinated form frequently predominates, with a correlation to tumor aggressiveness. On the other hand, 3-nitrotyrosine has been shown to be incorporated, by TTL, into the carboxy terminal end of detyrosinated alpha-tubulin. This reaction is not reversible by the carboxypeptidase enzyme. Cells cultured in 3-nitrotyrosine rich medium showed evidence of altered microtubule structure and function, including altered cell morphology, epithelial barrier dysfunction, and apoptosis. Bacterial homologs of TTL are predicted to form peptide tags. Some of these are fused to a 2-oxoglutarate Fe(II)-dependent dioxygenase domain.
Statistics
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PSSM-Id: 281171
View PSSM: pfam03133
Aligned: 20 rows
Threshold Bit Score: 234.15
Threshold Setting Gi: 1723548
Created: 21-Mar-2017
Updated: 23-May-2017
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q9VZ97 119 FSVDSGYrmhdNQMINHFPNHYELSRKD-LLVKNIKRYRKDLERDgnplaektesnnssgtrylyLDFVPTTFVLPADYN 197  fruit fly
Q9VGW2 126 ANLKNLGq---HQVVNHYPGCGYLTNKV---D------LCTTQLP----------------------FLPRAFRLPAERE 171  fruit fly
Q9VM92 325 VRHAEHYs--lDDARIKPPTPAEIVENQtFMVQSTDVLKSNAKFKvs-----------------eKVMAEYARLAGLYLD 385  fruit fly
Q9Y4R7 213 MDIDKD-----LEAPLYLTPEGWSLFLQ-RYYQVVHEGAELRHLD--------------------TQVQRCEDILQQLQA 266  human
Q9VM91 342 IDSCQHN----DIDFEDPPKIWEHDWDA-FLFQHQQLVNEDGRIQhd----------------ggQRLEPMVKSCLSLVD 400  fruit fly
P38584  81 IKTSPEL----AESCTWFPESYVIYPTN-LKTPVAPAQDGIHPPL----------------------HSSRTDEREFFLA 133  cattle
Q09512 370 QLSEENP----CGMINQFPFESCITVKD-LLAACAMRDPAKNDWY------------------------QLTYNLNTQLP 420  Caenorhabditis elegans
Q14166 346 KLSQERP----GVLLNQFPCENLLTVKD-CLASIARRAGGPEGPP----------------------WLPRTFNLRTELP 398  human
P38254 388 EQLDMDKl---MELPNNYFTNSYIYRKAlIRKHFLSHTIQTYTAKnpe--------------silKKAYLESFTIDLDYA 450  Saccharomyces cerevi...
Q10438 119 SLMEA------YELRQELEENATKNISE-KQWYILKPSMCDRAQG------------------------IRLFSTIEELQ 167  Schizosaccharomyces ...
Q9VZ97 198 MFVEEYRKFPL------------STWIMKPCGKSQGAGIFLINKLSKLKKwsreak------------------------ 241  fruit fly
Q9VGW2 172 EFLDYARENPQ-------------ALFVQKHNEHRHIKVRAPADIAFGS------------------------------- 207  fruit fly
Q9VM92 386 QIESLRPDYRWdg--------srNLWILKPGYQSRGIGIVIRSSLDDILQwt---------------------------- 429  fruit fly
Q9Y4R7 267 VVPQIDMEGDR------------NIWIVKPGAKSRGRGIMCMDHLEEMLKlvng-------------------------- 308  human
Q9VM91 401 KMKVHWPQYSLdg--------yqNMWIVKPANKCRGRGIILMDNLKKILGvvn--------------------------- 445  fruit fly
P38584 134 SYNRKKEEGEG------------NVWIAKSSAGAKGEGILISSDATELLDfi---------------------------- 173  cattle
Q09512 421 EFVARFQNRELng--------qhNVWIVKPWNLARGMDMTVTEDLNQIIR------------------------------ 462  Caenorhabditis elegans
Q14166 399 QFVSYFQQRERwg--------edNHWICKPWNLARSLDTHVTKSLHSIIR------------------------------ 440  human
P38254 451 EFLDDALDENWelrqelenesqdKWWIVKPSMSDKGQGIRVFKTIEDLQAifdsfddedseaeesgndddaddvngefmd 530  Saccharomyces cerevi...
Q10438 168 AIFDSFDDEES--------------ESEEAGLEEKGDITVAFNNKIVIS------------------------------- 202  Schizosaccharomyces ...
Q9VZ97 242 gpfhpQIAKESYVISRYIDNPLLIGG------KKFDLRLYVLVASF-RPLKAYLFKQG-FCRFCTVKYDt------sVTE 307  fruit fly
Q9VGW2 208 ---------NDSFVQEFVQRPYLVDG------HKFDIGVYVVITSV-NPLRVYIYTGDvLFRYCPVKYHp--------FD 263  fruit fly
Q9VM92 430 ----sNNQNKKYIVQKYIERPLLIYR------TKFDIRQYMLLTITdTKVSIWTYRDC-YLRFSSQEFT---------MD 489  fruit fly
Q9Y4R7 309 --npvVMKDGKWVVQKYIERPLLIFG------TKFDLRQWFLVTDW-NPLTVWFYRDS-YIRFSTQPFS---------LK 369  human
Q9VM91 446 ---lsIASKSRYVVQKYIERPLILFQ------TKFDIRQWFLITNT-QPLVVWFYRES-YLRFSSQEYS---------LS 505  fruit fly
P38584 174 -----dnQGQVHVIQKYLERPLLLEPg----hRKFDIRSWVLVD---HQFNIYLYREG-VLRTASEPYH--------MDN 232  cattle
Q09512 463 -----MIETGPKIVCEYIPRPLLFPRpdngnkVKFDLRYIVFLNGI-APVTAYVYNRF-WIRFAINEFS--------LSN 527  Caenorhabditis elegans
Q14166 441 -----HRESTPKVVSKYIESPVLFLRedv-gkVKFDIRYIVLLRSV-RPLRLFVYDVF-WLRFSNRAFA--------LND 504  human
P38254 531 nnkvnISQLRHFIIQEYLTNPLLLASmd---nRKFHIRCYVVCRG---DLQVFVYDRM-LALFAAKPFVpldpyaysVTD 603  Saccharomyces cerevi...
Q10438 203 -------QIRNFLVQKYISKPLLLDH------RKFHIRAYVLATG---ALSVYLFNEM-LCLLARDKYKk------pTPD 259  Schizosaccharomyces ...
Q9VZ97 308 LDNMYVHLTNVSVQKH------GGEYNTLHGGKWSVQNLALYLEG--TRGKEVTDRLFGAISWLIVHS--LRAVAPVMAS 377  fruit fly
Q9VGW2 264 AENVDKYIVGDDYLPTwevpslRKYYNRFGGSMRTVFEAYVRDQG--KDPAQIWPQVEHIVRTTIAAKekDIVNILRSYR 341  fruit fly
Q9VM92 490 DLRESIHLTNNSVQKRykn-ktNRDSRLPKNNMWSLDQFKNYLRIm-GAPDGSWSKTYNGFKQNLVAV--VMASLDETEL 565  fruit fly
Q9Y4R7 370 NLDNSVHLCNNSIQKHlen-scHRHPLLPPDNMWSSQRFQAHLQE--MGAPNAWSTIIVPGMKDAVIHa-LQTSQDTVQC 445  human
Q9VM91 506 NHHESVHLTNYAIQKKyt--ngKRDKRLPSENMWDCYSFQAYLRQ--IGKYNMWLERIFPGMRKAIVGc-MLASQENMDR 580  fruit fly
P38584 233 FQDKTCHLTNHCIQKEys--knYGKYEEGNEMFFEAFNRYLTSALn-ITLESSILLQIKHIIRSCLMS--VEPAISTKHL 307  cattle
Q09512 528 FEDVETHFTVFNYLDK--------EKILQMKCENFIETIEKAYPR--IQWSEVQKDINLTIRKAIEAAa-KEEAPRGVAP 596  Caenorhabditis elegans
Q14166 505 LDDYEKHFTVMNYDPD--------VVLKQVHCEEFIPEFEKQYPE--FPWTDVQAEIFRAFTELFQVAc-AKPPPLGLCD 573  human
P38254 604 LKDLECHLTNTCLQSK------------KKDKDSSVLEFDSIEEIpnERKSNIKEQIHSITNDVFLAA--VNVNRLNFQP 669  Saccharomyces cerevi...
Q10438 260 PDLLFSHLSNTCLQGD-------------NVEQSSIRDFWNTSIE---NKDDIFKSILNIIGDVFEAA--ATTQGIHFQP 321  Schizosaccharomyces ...
Q9VZ97 378 DRHCFECYGYDIII--------DNALKPWLVEVNASPSL-TSTTVNDRILKYKLIDNILS---VVLPPDGVPDV 439  fruit fly
Q9VGW2 342 THNFFDLMRFDLFI--------DEDLKVFLMEANMSPNLsSAHFKPNSLLYEQVLYSVFNlvgIRPLTSTSGAL 407  fruit fly
Q9VM92 566 LQNAFELYGCDFML--------DEHYNPILIEINSTPDL-SPSTEITARICPMVLKDCIR-----VVVDLPKNP 625  fruit fly
Q9Y4R7 446 RKASFELYGADFVF--------GEDFQPWLIEINASPTM-APSTAVTARLCAGVQADTLR-----VVIDRMLDR 505  human
Q9VM91 581 RPNTFELFGADFMI--------CENFYPWLIEINSSPDL-GATTSVTARMCPQCLEDVVK-----VVIDRRTDP 640  fruit fly
P38584 308 PYQSFQLFGFDFMV--------DEELKVWLIEVNGAP---ACAQKLYAELCQGIVDIAIA---SVFPPPDAEQQ 367  cattle
Q09512 597 NVQSRAMYGVDIMLqh----gdNDVIKSTLLEINFMPDT-TRACQYYPDFADTVFETLFL---DEIDPTKVTPI 662  Caenorhabditis elegans
Q14166 574 YPSSRAMYAVDLMLkwdngpdgRRVMQPQILEVNFNPDC-ERACRYHPTFFNDVFSTLFL---D-QPGGCHVTC 642  human
P38254 670 LPNAFETYGVDFLI--------DSNYEVKLLEINAFPDF-KQTGKDLKNLIDELFDDTVKy--CVTPIFNENRN 732  Saccharomyces cerevisiae S...
Q10438 322 LENCFEIFGVDFLV--------DCESQVYLLEVNSYPDF-KQTGKNLSNIIENLFSAVVE---TAIIPFFESST 383  Schizosaccharomyces pombe ...
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