Conserved Protein Domain Family
SPX

?
pfam03105: SPX 
SPX domain
We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 residue long domain is found at the amino terminus of a variety of proteins. In the yeast protein SYG1, the N-terminus directly binds to the G-protein beta subunit and inhibits transduction of the mating pheromone signal. Similarly, the N-terminus of the human XPR1 protein binds directly to the beta subunit of the G-protein heterotrimer leading to increased production of cAMP. These findings suggest that all the members of this family are involved in G-protein associated signal transduction. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors PHO81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. The SPX domain of S. cerevisiae low-affinity phosphate transporters Pho87 and Pho90 auto-regulates uptake and prevents efflux. This SPX dependent inhibition is mediated by the physical interaction with Spl2 NUC-2 contains several ankyrin repeats pfam00023. Several members of this family are annotated as XPR1 proteins: the xenotropic and polytropic retrovirus receptor confers susceptibility to infection with murine xenotropic and polytropic leukaemia viruses (MLV). Infection by these retroviruses can inhibit XPR1-mediated cAMP signalling and result in cell toxicity and death. The similarity between SYG1, phosphate regulators and XPR1 sequences has been previously noted, as has the additional similarity to several predicted proteins, of unknown function, from Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae, and many other diverse organisms. In addition, given the similarities between XPR1 and SYG1 and phosphate regulatory proteins, it has been proposed that XPR1 might be involved in G-protein associated signal transduction and may itself function as a phosphate sensor.
Statistics
?
Aligned: 226 rows
Threshold Bit Score: 54.112
Threshold Setting Gi: 74852218
Created: 6-May-2019
Updated: 18-Jul-2019
Structure
?
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
EKC99484                 1 MKFGRYLEENATPEWKRAYIDYRGAKKAIK----RAAA-SREnqavdenndfsseedda-------------dngpsakp 62   Tric...
EGG14759                 1 MKFGKYLENNQVPEWKDKYVSYKQLRKNIK----RFKS-EIE-------------------------------------- 37   Cave...
Q86HQ3                   1 MKFRDYLKDNKVSHWRDKYIDYEYLKNLIDn---EYINaEIL-------------------------------------- 39   Dict...
Q54HI2                   1 MKFRDLLNDHIISHWRDKYIDYEYLKDLIDreyhNAPN-SLNnsmmidmsqsinntiaeqysvndissmvtkggindspt 79   Dict...
EGC32499                 1 MKFREYLNSHIVSHWRDKYLDYEYLKQLID----KEYNnAPNsldnsaiidftqs---------------------iadq 55   Dict...
Q54MJ9                   1 MKFGKYLQRRQVSAWKKKYVFYKSFKKQIK----SIKRaKTEeelyignesqiqi----------------------elt 54   Dict...
EGF84226                 1 MKFSHSITLNASPEWREHYLAYSHLKKLIY----AIEK------------------------------------------ 34   Batr...
EPB89568                 1 MKFAHSIVLNAVPEWLDKYVDYDRLKKMVY----QAEK-DQV-------------------------------------- 37   Muco...
KDE06348                 1 MKFAHSLVFNSVPEWTSHYLNYEALKAAIY----RFEK------------------------------------------ 34   Micr...
WGS:AAWC:PGTG_11800.3    1 MKFSHSLLFSTVPEWQSYYVSYDSLKATIY----KIEK------------------------------------------ 34   Pucc...
EKC99484                63 rspnspsalTRIISGSPRTPNTARSGKTPRTGPsplsprpkrsvgkspaagpsgtrvsetvrwagasgqtvtarsfesle 142  Tric...
EGG14759                38 ---------SIIKSNNNDSSSNHKLEN----------------------------------------------------- 55   Cave...
Q86HQ3                  40 ---------NSAIIDLPLSPEEQNNINKN--------------------------------------------------- 59   Dict...
Q54HI2                  80 tdievdetaDSPAIPSPIISHSNINSNNNNNGGtnsvgfshlhrqln-------------------------rqnsnlkn 134  Dict...
EGC32499                56 hhtsiinqlSESGSNNINTQDEDHIKSYFKNKTpqlqp------------------------------------------ 93   Dict...
Q54MJ9                  55 aipinksisKIPPPSQSSSSPSQS-------------------------------------------------------- 78   Dict...
EGF84226                35 ---------ATLGLDQL--------------------------------------------------------------- 42   Batr...
EPB89568                   -------------------------------------------------------------------------------- Muco...
KDE06348                   -------------------------------------------------------------------------------- Micr...
WGS:AAWC:PGTG_11800.3   35 ---------DQASSHQSSLRLS---------------------------------------------------------- 47   Pucc...
EKC99484               143 shrlsVPDSRDRHHADTQPSPGYGATGTSPPLDDNGVQFPPPLDLGepgippspdlkssqqtaflepsdlnkerlkgrvd 222  Tric...
EGG14759                56 -----EQDNNNNNNISGQLKRTYSSIILNKSNLFNPLPSTTPSSPI---------------------------------- 96   Cave...
Q86HQ3                  60 -----NNNNNNNNNNNNNNNNNNNNINNNNNNNNTLKDGEQTNSNNisipnq---------------------------- 106  Dict...
Q54HI2                 135 ssnsiNLLKADDSNNIEKHVKSYFDQRNNNNNINNINNNNNNNSNNsnnsnnnktikntrniiad--------------- 199  Dict...
EGC32499                94 -phsqPLPPPPPLQQQQQQSQSSPTSPQQLNIPNHHLHHNRFHSHNirdedtt--------------------------- 145  Dict...
Q54MJ9                  79 -----QSQIEIPLQSIEPTTTTTTTAATSSSSSSTTTTNVNSKTIIns-------------------------------- 121  Dict...
EGF84226                43 -----PVDHHDLENGTLPPAHIYQGASLETPQADSIRDEAPSEQTP---------------------------------- 83   Batr...
EPB89568                38 -----DQQNQDAKSQIEKVETGLQGEKQSWEMNQQISSGSDQLVVNs--------------------------------- 79   Muco...
KDE06348                35 -----QQLAPLPT--------EYTDVERGADSPLLGNGNGASAKPA---------------------------------- 67   Micr...
WGS:AAWC:PGTG_11800.3   48 -----SQPSSHPSRREPAQSDSSNNELQDHENNESTGLLQNHDGSP---------------------------------- 88   Pucc...
EKC99484               223 targasgatsgatsgaasgatsgsasgaasgsrspaissipetpaegeergtsseggpsspdapdtpltqssrkhhgHTK 302  Tric...
EGG14759                   -------------------------------------------------------------------------------- Cave...
Q86HQ3                 107 ------------------------------------------------------------------------mapqeSSG 114  Dict...
Q54HI2                 200 ----------------------------------------------------------ddggnedttlgspfsspsiGSP 221  Dict...
EGC32499               146 ----------------------------------------------------------------------lgsaspfSNS 155  Dict...
Q54MJ9                 122 ---------------------------------------------------------------------------sgGSI 126  Dict...
EGF84226                84 ------------------------------------------------------------------------------LL 85   Batr...
EPB89568                   -------------------------------------------------------------------------------- Muco...
KDE06348                   -------------------------------------------------------------------------------- Micr...
WGS:AAWC:PGTG_11800.3   89 -------------------------------------------------------------------------------E 89   Pucc...
EKC99484               303 GSPSKFSFKSPKThasvgvsqsstadapsqkkSLRNMKLPSPkiqpkqpqsmdelyatlnpDERAFFD--HLDKQLDKVE 380  Tric...
EGG14759                97 -PRQQQQQQLPNP------------------------------------------------VDLQLLL--INQKQMQSHN 125  Cave...
Q86HQ3                 115 EDEDDENENGNGVv-----------------gDEDGGDDDEI-------------------EMDEL----VIDDNGEIIN 154  Dict...
Q54HI2                 222 PMSSPSPKMLKQElhspl---------ltsekDEDEEEEGEE-------------------EEDIEMEqlELDDHDKNTT 273  Dict...
EGC32499               156 SVGSPRPKAKSEL--------------------NSPLLSDQD-------------------NDEIEMEqiNLDSSNNNNN 196  Dict...
Q54MJ9                 127 KSNNSNPLSQSSIi------------------QRGPVIIRENni---------------erEEKKFDS--MLQEEFDKVN 171  Dict...
EGF84226                86 PAGMESVTAVGKLsl--------------------------------------------qdANDFFER--ALDEELDKIK 119  Batr...
EPB89568                80 -------------------------------------------------------------ENEAFLQ--ELDRQLDKVT 96   Muco...
KDE06348                68 -----------------------------------------S-------------------NDKLFTR--LLDQELNKIT 85   Micr...
WGS:AAWC:PGTG_11800.3   90 QATSIIELQPNPLnl--------------------------------------------tpSDRIFLE--LLSQELAKVE 123  Pucc...
EKC99484               381 TFYSAREQEATRRFS--QLKDQLNAL-AE------HRRI---------FHEQFpggqheweskvgrvtkn-----ahvpi 437  Tric...
EGG14759               126 D-SSLYNQYQSNQFT----KTTLTRL---------QEIQ----------------------------------------- 150  Cave...
Q86HQ3                 155 ITATAGSRVKVKK----GINKGVKRL---------KKIA---------KNTNNrlsrmv--------------------- 191  Dict...
Q54HI2                 274 INMTANGSRGNLRKGmnQFVKTIPQNiKQ------LNSQ---------ISNSFlkf------------------------ 314  Dict...
EGC32499               197 TNNKNNNNNNNIILN--NKNESYANL---------KKAG---------IYNLNknlsy---------------------- 234  Dict...
Q54MJ9                 172 TFFKQQEDEFIHQFN--DIKQKVVAM---------SEIC---------KNSSSkslkd---------------------- 209  Dict...
EGF84226               120 NFYTEKELDLLADVQ--ALINEIRSV-EKyeesylVGLS---------DAFDSsqvpslhlmspalpppqvg--lesvre 185  Batr...
EPB89568                97 TFFTAKEQEFLQDAR--DLRSEFDT----------FQIP---------LPDSGnhpntttsfaetsalerld-sglhksq 154  Muco...
KDE06348                86 EFYVAKERELLGDLA--LLQADVERIeDEtshlsgQGMT---------SGASDseddrqddde----------------- 137  Micr...
WGS:AAWC:PGTG_11800.3  124 EFYQSKQQELFDELN--GLINEIETIeKQgmqliqDSFNqsarstdeaIDDEDddeddeeeedngrgeegtsnqprrvsp 201  Pucc...
EKC99484               438 lakavqhlhgrnPFHNHt-DDENAGGSGANSANGGngangtrr-------------------rspagmpGASNGNTPSNG 497  Tric...
EGG14759               151 ------------EEFIQlcIGEAEKNSKSEKEELmnqmee---------------------------------------- 178  Cave...
Q86HQ3                 192 -------srspkNKGLG--SSSEIEGDEFQLDGGGissqie----------------------mvasnnITGATVFVPQE 240  Dict...
Q54HI2                 315 ---------ggmVKGDKs-NDKNNDKSNDKNNNKNnknnnnnnnl---------------ndednfdmmTSQIEKVLNNK 369  Dict...
EGC32499               235 --------nnlnKNISFn-NLNQLHNSGNINNSVNmnksmshgnfdglsrtsshlnlevltsqiekvmeSRENMELMESG 305  Dict...
Q54MJ9                 210 --------aiseDSPRLg-KFSHLFYNPSIIKKRNssnlts----------------------saikddGVGGASSNSQR 258  Dict...
EGF84226               186 eplsysiacgsnPSAASa-TTTMAFLGSTVDATKTsptssase-------------------smmedlsQSKPSLIKRAT 245  Batr...
EPB89568               155 trdsyttfnssiAGSDI--DDDEPSTSTAATASLPvynnaqlrysttdtlhh-qpnaisndsddskklnLPPLAEKFSSA 231  Muco...
KDE06348               138 --egeegvaartSKMVKg-AIEQVFSNPRNYDAANrearkarrkrag-----------sfarsfeglsgGEGSAGRRRAR 203  Micr...
WGS:AAWC:PGTG_11800.3  202 stkrqqllksasIFGILp-ILGFSSKASRPTRSTSlsrsrgssknptphrvtknqnahlidvnpstsvtVTKPTSPTSPL 280  Pucc...
EKC99484               498 GTAYSta------------vsSVRQPEGGSngsngs--gsegsSGDNDLSGT-----TAAGSTRVLPGkp-------kqF 551  Tric...
EGG14759               179 ---------------teetleSQTISIQM----------------DQLGVED-----DETELEDGANN-----------N 211  Cave...
Q86HQ3                 241 TFQDG----------------FIDQVNKVDiff-------vdrYRKTKFKCV-----DLVGMIPFLSD------------ 280  Dict...
Q54HI2                 370 DNMKLmesc---------tftTKENFQTAFv------------DQVNKVDSF-----FVERYRKTKEKcvelcnmipflS 423  Dict...
EGC32499               306 IIQQQenf----------qtiFIDQVNKV----------------DTFFVER-----YRKTKKKCVELcnmi----pflS 350  Dict...
Q54MJ9                 259 YAQSSp--------------sSSSSSSPS----------------AMAVKAI-----AHAANAETYWN-----------P 292  Dict...
EGF84226               246 TIGPIlhs-----------snPNISLQSSVspnatdrrrvyseNFDDAIGMT-----PDASRPGYLTFl---------iW 300  Batr...
EPB89568               232 YAPTRsntisasnfrsrrgsrIERTLSQHDampa-----yaedVANKPPTRI-----MQAIGLRFRRRr----------G 291  Muco...
KDE06348               204 ALSNAsats--------gdllGIDEIQEEGqgqngg--arsseSGRRAVRKIgsklfGTPSRKRDTSRfiags--taneD 271  Micr...
WGS:AAWC:PGTG_11800.3  281 APQFAprasl------sldvsTRAIQADTSqhrsai--skwlpHSPKTSRSLslgsqTAHRRPSQNVPapnl---sefwM 349  Pucc...
EKC99484               552 DPERYQKYKK--------ELRTAVVEYYRQLELIKNYRILNLTGFRKALKKFEKVTGIHCLDLYTDER 611  Trichosporon asa...
EGG14759               212 NNNNIDSSKI-------------ILEYYKFLNLLKNYKIFNYTGFIKILKKAEKNLELNLNDRILTRL 266  Cavenderia fasci...
Q86HQ3                 281 -NEQLRTIRNi------dYVKQGFHDNYHYLESLESYKNLNLDGFYKILDKYEKINPR-----IAKEC 336  Dictyostelium di...
Q54HI2                 424 TNEQLRTIRNi------eFVKQGFQDNYHYLESLEAFKELNIKGFKKVLEKYEKKNRI-----ISSEC 480  Dictyostelium di...
EGC32499               351 TKEQYRTIRNi------dYVKQGLHDNYHYLEMLESFKDVNIKGFNKVLNKYEKKNRL-----IANEC 407  Dictyostelium pu...
Q54MJ9                 293 GSLKLGKIRR--------SLKRAMEENYREIQALKEYTSLNMIAFRKIFKKYDKVLQSDSSVDGMKLV 352  Dictyostelium di...
EGF84226               301 SSKGLRNHRQ--------RFQKRVIGLFVVLCELRDYVDIHETGFSKVLKKYEKVVGAKLKHSYMAKV 360  Batrachochytrium...
EPB89568               292 SHVSLHQAENvnefnqyyNFRVRCAATFIALTELKAYASTNRTAFDKILKKWDKVTNANLRQVYFQKI 359  Mucor circinello...
KDE06348               272 DDDGVFGGASdwaidtriMYKRRIAALFTSLSELKQYVDLNYTGLSKVLKKYDKITNSKLRSKYMSEV 339  Microbotryum lyc...
WGS:AAWC:PGTG_11800.3  350 SNVEFIHDSRv-------FFKRRITQLFVNLNSLKTYIDLNFTAFRKILKKYDKVFGSSLSSHFLEEC 410  Puccinia gramini...
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap