2W42,1YVU


Conserved Protein Domain Family
Piwi

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pfam02171: Piwi 
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Piwi domain
This domain is found in the protein Piwi and its relatives. The function of this domain is the dsRNA guided hydrolysis of ssRNA. Determination of the crystal structure of Argonaute reveals that PIWI is an RNase H domain, and identifies Argonaute as Slicer, the enzyme that cleaves mRNA in the RNAi RISC complex. In addition, Mg+2 dependence and production of 3'-OH and 5' phosphate products are shared characteristics of RNaseH and RISC. The PIWI domain core has a tertiary structure belonging to the RNase H family of enzymes. RNase H fold proteins all have a five-stranded mixed beta-sheet surrounded by helices. By analogy to RNase H enzymes which cleave single-stranded RNA guided by the DNA strand in an RNA/DNA hybrid, the PIWI domain can be inferred to cleave single-stranded RNA, for example mRNA, guided by double stranded siRNA.
Statistics
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PSSM-Id: 308014
View PSSM: pfam02171
Aligned: 16 rows
Threshold Bit Score: 231.831
Threshold Setting Gi: 37999509
Created: 16-Jul-2016
Updated: 4-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2W42_B       110 GIMLVLPE-YNTPL-YYKLKSYLINS--IPSQFMRYDILSNRn--------lTFYVDNLLVQFVSKLGGK---PWILNVD 174
gi 11386877  500 YLFFILDK-NSPEP-YGSIKRVCNTMlgVPSQCAISKHILQSk---------PQYCANLGMKINVKVGGIn-cSLIPKSN 567
gi 74960296  594 TFVFIITD-DSITT-LHQRYKMIEKDtkMIVQDMKLSKALSVina-----gkRLTLENVINKTNVKLGGSn--YVFVDAK 664
gi 2496894   650 DILVGIAR-EKKPD-VHDILKYFEESigLQTIQLCQQTVDKMmgg----qggRQTIDNVMRKFNLKCGGTnffVEIPNAV 723
gi 74962267  397 MLVVMLAD-DNKTR-YDSLKKYLCVEcpIPNQCVNLRTLAGKskdggenknlGSIVLKIVLQMICKTGGA---LWKVNIP 471
gi 74873032  555 IVMVVMRS-PNEEK-YSCIKKRTCVDrpVPSQVVTLKVIAPRqqkp---tglMSIATKVVIQMNAKLMGA---PWQVVIP 626
gi 33112431  538 LILCLVPN-DNAER-YSSIKKRGYVDraVPTQVVTLKTTKNRs--------lMSIATKIAIQLNCKLGYT---PWMIELP 604
gi 74716803  555 IVVCLLSS-NRKDK-YDAIKKYLCTDcpTPSQCVVARTLGKQqt-------vMAIATKIALQMNCKMGGE---LWRVDIP 622
gi 74965532  673 TIVFGIIA-EKRPD-MHDILKYFEEKlgQQTIQISSETADKFmrd----hggKQTIDNVIRKLNPKCGGTnflIDVPESV 746
gi 37999509  426 CFALIIGKeKYKDNdYYEILKKQLFDlkIISQNILWENWRKDd--------kGYMTNNLLIQIMGKLGIK---YFILDSK 494
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2W42_B       175 Pe--------------------kGSDIIIGTGATr--------iDNVNLFCFAMVFk-KDGTMLWNEISPIVTSSEYLTY 225
gi 11386877  568 Plgnvp-------------tlilGGDVYHPGVGA----------TGVSIASIVASVd-LNGCKYTAVSRSQPRHQEVIEG 623
gi 74960296  665 Kqlds--------------hliiGVGISAPPAGTkyame-nkgvLNPNVIGYAYNA--QHNQEFSGDFVLNSASQDTLAP 727
gi 2496894   724 RgkavcsnnetlrkkllehvqfiGFEISHGASRTlfdrsrsqmdGEPSVVGVSYSLt---NSTQLGGFTYLQTQKEYKLQ 800
gi 74962267  472 Lkst----------------mivGYDLYHDSTLK-----------GKTVGACVSTTs-NDFTQFYSQTRPHENPTQLGNN 523
gi 74873032  627 Lhgl----------------mtvGFDVCHSPKNK-----------NKSYGAFVATMdqKESFRYFSTVNEHIKGQELSEQ 679
gi 33112431  605 Lsgl----------------mtiGFDIAKSTRDR-----------KRAYGALIASMdlQQNSTYFSTV-TECSAFDVLAN 656
gi 74716803  623 Lklv----------------mivGIDCYHDMTAG-----------RRSIAGFVASIn-EGMTRWFSRCIFQDRGQELVDG 674
gi 74965532  747 GhrvvcnnsaemraklyaktqfiGFEMSHTGARTrfdiqkvmfdGDPTVVGVAYSL--KHSAQLGGFSYFQESRLHKLTN 824
gi 37999509  495 Tpyd----------------yimGLDTGLGIFGN------------HRVGGCTVVYdsEGKIRRIQPIETPAPGERLHLP 546
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2W42_B       226 -LKSTIKKVVYGFKKSNPDwDVEKLTLHVSG----KRPKMKDgETKILKETVE-------ELKKQEMvsRDVKYAILHLN 293
gi 11386877  624 -MKDIVVYLLQGFRAMTKQ-QPQRIIYFRDGtsegQFLSVINdELSQIKEACH-------SLSPKYN--PKILVCTTQKR 692
gi 74960296  728 -IEDIVMHSLNEYQKFHDGgLPRRVIVYRTGtsegNHGSIMAyEIPLARAAMR-------DFSPDIQ----LVYIVVSKD 795
gi 2496894   801 kLDEFFPKCVRSYKEHSKT-LPTRIVIYRVGagegNFNRVKE-EVEEMRRTFD-------KIQPGYR--PHLVVIIAQRA 869
gi 74962267  524 -LTHFVRKSLKQYYDNNDKtLPSRLILYRDGagdgQIPYIKNtEVKLVRDACDavtdkaaELSNKVQekIKLAFIIVTKR 602
gi 74873032  680 -MSVNMACALRSYQEQHRS-LPERILFFRDGvgdgQLYQVVNsEVNTLKDRLDei---ykSAGKQEG--CRMTFIIVSKR 752
gi 33112431  657 tLWPMIAKALRQYQHEHRK-LPSRIVFYRDGvssgSLKQLFEfEVKDIIEKLKte---yaRVQLSP---PQLAYIVVTRS 729
gi 74716803  675 -LKVCLQAALRAW-NSCNEyMPSRIIVYRDGvgdgQLKTLVNyEVPQFLDCLK-------SIGRGYN--PRLTVIVVKKR 743
gi 74965532  825 -LQEKMQICLNAYEQSSSY-LPETVVVYRVGsgegDYPQIVN-EVNEMKLAAR-------KKKHGYN--PKFLVICTQRN 892
gi 37999509  547 ----YVIEYLEN--KANIDmENKNILFLRDG-------FIQNsERNDLKEISK-------ELN------SNIEVISIRKN 600
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2W42_B       294 ETHPFWVMGDPN------------------------NRFHPYEGTKVklsSKRYLLTLLQPYLKRNGLEMVTPIKPLSVE 349
gi 11386877  693 HHARFFIKNKSDg----------------------dRNGNPLPGTII---EKHVTHPYQYDFYLISHPSLQGVSVPVHYT 747
gi 74960296  796 HSFRFFKPDLASlasrpqatsstasrhsampaapkaWDLNIAPGILV---DSIVTNPACKQFFLNSHITLQGTAKTPLYT 872
gi 2496894   870 SHARVFPSCISGnr---------------------aTDQNIPSGTCV---ENVLTSYGYDEFILSSQTPLIGTVRPCKYT 925
gi 74962267  603 VNMRILKQGSSSk-----------------------SAINPQPGTVV---DTTVTRPERMDFYLVPQFVNQGTVTPVSYN 656
gi 74873032  753 INSRYFT-----------------------------GHRNPVPGTVV---DDVITLPERYDFFLVSQAVRIGTVSPTSYN 800
gi 33112431  730 MNTRFFLN-----------------------------GQNPPPGTIV---DDVITLPERYDFYLVSQQVRQGTVSPTSYN 777
gi 74716803  744 VNTRFFAQSGGR-------------------------LQNPLPGTVI---DVEVTRPEWYDFFIVSQAVRSGSVSPTHYN 795
gi 74965532  893 SHIRVFPEHINErg--------------------ksMEQNVKSGTCV---DVPGASHGYEEFILCCQTPLIGTVKPTKYT 949
gi 37999509  601 NKYKVFTSDYRI------------------------GSVFGNDGIFLphkTPFGSNPVKLSTWLRFNCGNEEGLK---IN 653
                        330       340       350       360       370       380
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
2W42_B       350 IVSDN--WTSEEYyHNVheildeIYYLSKMNWRGF----RSRNLPVTVNYPKLVAGIIANVNR 406
gi 11386877  748 VLHDEiqMPPDQF-QTL------CYNLCYVYARAT----SAVSLVPPVYYAHLVSNLARYQDV 799
gi 74960296  873 VLADDakVSMTAL-EDI------TYKLCHLHQIVG----LPTSLPTPLYVANEYAKRGRNLWN 924
gi 2496894   926 ILVNDakWSKNEL-MHL------TYFRAFGHQVSY----QPPSVPDVLYAAENLAKRGRNNYK 977
gi 74962267  657 IIHDDtgLGPDKH-QQL------AFKLCHLYYNWQ----GTVRVPAPCQYAHKLAFLTAQSLH 708
gi 74873032  801 VISDNmgLNADKL-QML------SYKMTHMYYNYS----GTIRVPAVCHYAHKLAFLVAESIN 852
gi 33112431  778 VLYSSmgLSPEKM-QKL------TYKMCHLYYNWS----GTTRVPAVCQYAKKLATLVGTNLH 829
gi 74716803  796 VIYDNsgLKPDHI-QRL------TYKLCHIYYNWP----GVIRVPAPCQYAHKLAFLVGQSIH 847
gi 74965532  950 IIVNDcrWSKNEI-MNV------TYHLAFAHQVSY----APPAIPNVSYAAQNLAKRGHNNYK 1001
gi 37999509  654 ----------ESI-MQL------LYDLTKMNYSALygegRYLRIPAPIHYADKFVKALGKNWK 699
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