5GUH,1SI2,1VYN,2XFM,3QIR


Conserved Protein Domain Family
PAZ

?
pfam02170: PAZ 
Click on image for an interactive view with Cn3D
PAZ domain
This domain is named PAZ after the proteins Piwi Argonaut and Zwille. This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerization. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteristic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.
Statistics
?
PSSM-Id: 334832
View PSSM: pfam02170
Aligned: 245 rows
Threshold Bit Score: 47.5492
Threshold Setting Gi: 568255589
Created: 19-Mar-2017
Updated: 23-May-2017
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
5GUH_A         332 NYKKIFLEDVVGKIVMTDYn------kRTYRVDDVAWNVSPKSTFKMRde-------nITYIEYYYKKYNLRIQ--DPGQ 396
gi 75028686    308 VQKKKFNREVAGLKVETRHcs----cpRRFRVARCTWKPTENISFHLSetagnqdskpLSLVEYYKRRYNIDLtykH--L 381
gi 308501863   411 FESRIFGDSIRGMKIRATHrpn---aiRVYKVNSLQ-LPADKLMFQGIdeeg--revvCSVADYFSEKYG-PLkypK--L 481
gi 968078793   811 RLYPTIEDFLKGIFVIYEPpscfnsapRVFKVNGLTSSSARYQQFQLDnk-------tLTVEQYFQSR-NYSLR--YPNL 880
gi 195153098   409 YRRSDIESFLSDINIIYDPpacfgsapRVFRVNGLSKAPASTQTFELDgk-------eTTVAKYFKSR-KYDLkfpN--L 478
gi 1058272042  194 DRRYKIESFLKGMNIVYDPpacfasapRVFRVNGLSKFPASSQKFELDgk-------qTTVAEYFRSR-KYNLK--YPNL 263
gi 1058272092  138 SRRSDIDAHLKGISVAYEPpksflsatRVYKVNALTQYPASRQAFNCDgt-------kVTVAAYFKSR-GHALrfpN--L 207
gi 964138040   421 QSRRFIEPFLKGINIVYTPptsfgtasRVYKVNGLSAYPSNKQTFVLEgk-------tLTVSDYFKSR-NYVLkypS--L 490
1VYN_A          28 YSRRFLEPFLRGINVVYTPpqsfqsapRVYRVNGLSRAPASSETFEHDgk-------kVTIASYFHSR-NYPLkfpQ--L 97
gi 339256252    52 EHLSLLIPELKGVCIHTTHrn----qdRIYRIKDI-LSTAVSMKFERDgk-------eVSVAEYFHDVYG-PLkylN--L 116
                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
5GUH_A         397 PLLISRSkpReiraglpeLIYLVPELCRqtgLSDEMRANFKLmrSLDVHTKI 448
gi 75028686    382 PCIEVGRt-R--------ECILPLELCY---VVSGQRCIKKL--NEQQIANL 419
gi 308501863   482 PCLHVGPptR--------NIFLPMEHCL---IDSPQKYNKKM--TEKQTSAI 520
gi 968078793   881 RCLYVGPldR--------NIFLPIELCR---IEDCQSLNRKD--GANQVAAM 919
gi 195153098   479 LCLHVGPplK--------NIYLPIELCR---IDDGQTMKRKD--TAARVAAM 517
gi 1058272042  264 LCLHVGPplK--------NIYLPIELCR---IEDGQALNRKD--GANQVAAM 302
gi 1058272092  208 LCLHVGSpqM--------SVYLPIELCR---IEEKQALNRKD--SKVQSAGi 246
gi 964138040   491 QCLHVGPpvK--------NIYVPIELCH---IEAGQALNRKD--GATQVANM 529
1VYN_A          98 HCLNVGSsiK--------SILLPIELCS---IEEGQALNRKD--GATQVANM 136
gi 339256252   117 PLVQVGSksK--------PIYFPVELCQ---VANCQRYNKKL--KACQTTSI 155
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap