5GUH,1SI2,1VYN,2XFM,3QIR


Conserved Protein Domain Family
PAZ

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pfam02170: PAZ 
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PAZ domain
This domain is named PAZ after the proteins Piwi Argonaut and Zwille. This domain is found in two families of proteins that are involved in post-transcriptional gene silencing. These are the Piwi family and the Dicer family, that includes the Carpel factory protein. The function of the domains is unknown but has been suggested to mediate complex formation between proteins of the Piwi and Dicer families by hetero-dimerization. The three-dimensional structure of this domain has been solved. The PAZ domain is composed of two subdomains. One subdomain is similar to the OB fold, albeit with a different topology. The OB-fold is well known as a single-stranded nucleic acid binding fold. The second subdomain is composed of a beta-hairpin followed by an alpha-helix. The PAZ domains shows low-affinity nucleic acid binding and appears to interact with the 3' ends of single-stranded regions of RNA in the cleft between the two subdomains. PAZ can bind the characteristic two-base 3' overhangs of siRNAs, indicating that although PAZ may not be a primary nucleic acid binding site in Dicer or RISC, it may contribute to the specific and productive incorporation of siRNAs and miRNAs into the RNAi pathway.
Statistics
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PSSM-Id: 334832
View PSSM: pfam02170
Aligned: 245 rows
Threshold Bit Score: 47.5492
Threshold Setting Gi: 568255589
Created: 19-Mar-2017
Updated: 23-May-2017
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
5GUH_A        332 NYKKIFLEDVVGKIVMTDYn------kRTYRVDDVAWNVSPKSTFKMRde-------nITYIEYYYKKYNLRIQ--DPGQ 396  domestic silk...
Q9XVI3        308 VQKKKFNREVAGLKVETRHcs----cpRRFRVARCTWKPTENISFHLSetagnqdskpLSLVEYYKRRYNIDLtykH--L 381  Caenorhabditi...
XP_003113116  411 FESRIFGDSIRGMKIRATHrpn---aiRVYKVNSLQ-LPADKLMFQGIdeeg--revvCSVADYFSEKYG-PLkypK--L 481  Caenorhabditi...
XP_002066509  811 RLYPTIEDFLKGIFVIYEPpscfnsapRVFKVNGLTSSSARYQQFQLDnk-------tLTVEQYFQSR-NYSLR--YPNL 880  Drosophila wi...
XP_002017467  409 YRRSDIESFLSDINIIYDPpacfgsapRVFRVNGLSKAPASTQTFELDgk-------eTTVAKYFKSR-KYDLkfpN--L 478  Drosophila pe...
AOF40109      194 DRRYKIESFLKGMNIVYDPpacfasapRVFRVNGLSKFPASSQKFELDgk-------qTTVAEYFRSR-KYNLK--YPNL 263  Drosophila ps...
AOF40120      138 SRRSDIDAHLKGISVAYEPpksflsatRVYKVNALTQYPASRQAFNCDgt-------kVTVAAYFKSR-GHALrfpN--L 207  Drosophila ps...
XP_014764154  421 QSRRFIEPFLKGINIVYTPptsfgtasRVYKVNGLSAYPSNKQTFVLEgk-------tLTVSDYFKSR-NYVLkypS--L 490  Drosophila an...
1VYN_A         28 YSRRFLEPFLRGINVVYTPpqsfqsapRVYRVNGLSRAPASSETFEHDgk-------kVTIASYFHSR-NYPLkfpQ--L 97   fruit fly
XP_003370499   52 EHLSLLIPELKGVCIHTTHrn----qdRIYRIKDI-LSTAVSMKFERDgk-------eVSVAEYFHDVYG-PLkylN--L 116  Trichinella s...
5GUH_A        397 PLLISRSkpReiraglpeLIYLVPELCRqtgLSDEMRANFKLmrSLDVHTKI 448  domestic silkworm
Q9XVI3        382 PCIEVGRt-R--------ECILPLELCY---VVSGQRCIKKL--NEQQIANL 419  Caenorhabditis elegans
XP_003113116  482 PCLHVGPptR--------NIFLPMEHCL---IDSPQKYNKKM--TEKQTSAI 520  Caenorhabditis remanei
XP_002066509  881 RCLYVGPldR--------NIFLPIELCR---IEDCQSLNRKD--GANQVAAM 919  Drosophila willistoni
XP_002017467  479 LCLHVGPplK--------NIYLPIELCR---IDDGQTMKRKD--TAARVAAM 517  Drosophila persimilis
AOF40109      264 LCLHVGPplK--------NIYLPIELCR---IEDGQALNRKD--GANQVAAM 302  Drosophila pseudoobscura
AOF40120      208 LCLHVGSpqM--------SVYLPIELCR---IEEKQALNRKD--SKVQSAGi 246  Drosophila pseudoobscura
XP_014764154  491 QCLHVGPpvK--------NIYVPIELCH---IEAGQALNRKD--GATQVANM 529  Drosophila ananassae
1VYN_A         98 HCLNVGSsiK--------SILLPIELCS---IEEGQALNRKD--GATQVANM 136  fruit fly
XP_003370499  117 PLVQVGSksK--------PIYFPVELCQ---VANCQRYNKKL--KACQTTSI 155  Trichinella spiralis
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