2PPQ,1ZYL


Conserved Protein Domain Family
APH

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pfam01636: APH 
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Phosphotransferase enzyme family
This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.
Statistics
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PSSM-Id: 279908
View PSSM: pfam01636
Aligned: 156 rows
Threshold Bit Score: 50.2032
Threshold Setting Gi: 81554421
Created: 2-Aug-2016
Updated: 4-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2PPQ_A       26 SYKGIAEG-VENSNFLLHTTK-DPLI-LTLYEKR-VEKNDLPFFLGLXQHLAAKGLscplPLPR-K------Dge--lLG 92
1ZYL_A       30 GLTPLNS--YENRVYQFQDEDrRRFV-VKFYRPErWTADQILEEHQFALQLVNDEVp--vAAPVaF------Ngq--tLL 96
gi 76363316  30 gLTPLNS--YENRVYQFQDEDrRRFV-VKFYRPErWSVDQIREEHQFALELVKDEV----PVAApL------AfngqtLL 96
gi 81442334  36 DARVLALNsYENRVYQVGIEDaQPLI-AKFYRPGrWSDAAILEEHAFTAELADCEVp--vVAPMqH------Ddr--tLF 104
gi 81541896  25 DARVLALNsYENRVYQVGIEDgEPLI-AKFYRPDrWSDAAIREEHAFSAELAECEVp--vVAPLsR------Dge--sLF 93
gi 81656586  31 SLLALNS--YENRVYQVGLDDaAPLV-AKFYRPArWSDAAILEEHAFSLQLAEREIpa-vP-PLaF------Ggr--tLH 97
gi 81506458  37 DGRMFALNsYENRVYQAGIEDgAPVV-VKFYRPGrWNDAQIVEEHAFTAELAAAEIp--vVAPLeI------Dgr--tLH 105
gi 12230805  64 KGCRFWHR-GLSDVYLVETLA-DDYI-LRISHQHwRTESEIQFELELLNFLADRDV----PVAApL------Rhr--dGG 128
gi 12230798  30 DQVRFLAD-AENYVYECMKDN-QPYI-LKITHTIrRSSDYMMGEMEWLRHLAIGGI----SVAKpLpslngkDve--aVP 100
gi 267043    38 gLEFFPLG-EDSWSYRCGP----LWIsVRRDLDG-----HFPGAYEVSLLLSESGKg---YVLApL------Pgr--nGR 96
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2PPQ_A       93 ELSGR-------PAALISFleGXWLRKpea---------khcREVGKALAAXHLA-------SEGFEIKRp----NALS- 144
1ZYL_A       97 NHQGF-------YFAVFPSvgGRQFEAdnidqme--avgrylGRMHQTGRK--QLf-----iHRPTIGLNe----YLIE- 155
gi 76363316  97 AHQGY-------HYAIFPSvgGRQFEAdni---------dqmEAVGRYLGRLHQTg-----rKRPFTFRPdiglaEYLF- 154
gi 81442334 105 EHKGF-------RFTLFPRrgGHAPEpgnl---------dqlYRLGQLLGRLHAVg-----aSKPFKHREa----LAVDn 159
gi 81541896  94 AFSGF-------RFALFPRrgGRAPEpgnl---------dqlYRLGQLLGRLHAVg-----aTRPFEHREa----LAVDn 148
gi 81656586  98 GHGGF-------RFALFERrgGRVPELdrd---------etlQWVGRFLGRIHALgrtegyrHRPTLDMQs----FGRE- 156
gi 81506458 106 AFDRW-------HFAVFPRcaGRVPAIdrd---------dtlEWMGRFLGRIHAV-------GARRPFMAr----PTLD- 157
gi 12230805 129 YALEInapegkrYASLFPYapGGVAIGdlsk--------tqgFLLGEMLAQLHQTaq----rFKPSAHRP-------PL- 188
gi 12230798 101 DGNGGs-----fLLRVYEKapGQKVDEsdwnetlfyelgrytGSMHSLTKSYKLSnp----aFKRQEWDEe----EQLK- 166
gi 267043    97 VVHRIad----fPVVVFPYveSATTPPappt-------qeqiDLLIARLGEVHAFep---paSRPVDVPTe----DFRF- 157
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2PPQ_A      145 -----VD----------------------GWKVLWDKsEERADEVEKg---LREEIRP-EIDYLAAHWPK---------- 183
1ZYL_A      156 -----PR----------------------KLFEDA---TLIPSGLKA----AFLKATDeLIAAVTAHWRE---------- 191
gi 76363316 155 -----EP----------------------RQVFED-A-ALIPSGQKA----AFLKATDtLLSAVTECWRT---------- 191
gi 81442334 160 fghasLN----------------------TLLEGNFVpHELLPAFESv--aR--DLLKrVEDIYARTPH----------- 202
gi 81541896 149 fghasLA----------------------TLLEGNFIpRSLLPAYESv--aR--DLLKr-LDALFAEVP----------- 190
gi 81656586 157 -----PV----------------------DFVLGG---GWLPPELEAvyrgVAEQALQgVARCFERAGP----------- 195
gi 81506458 158 -----ID----------------------TFGVQSRD-WLLEHDFIPp---DLLPAWRsVADAALDGVRRcyd----rag 202
gi 12230805 189 -----TL----------------------SYLLDD---SLHTIAPFL-----HHRLEE-WRCLIDISMAIktqltsipth 232
gi 12230798 167 -----LR----------------------KYVPED------QIKVFQ-----QADSLMnELRRLPKSQD----------- 197
gi 267043   158 -----PFendldkavqaalngdaaaggpyAGLLAE---RVDRCQGFLa--eLREEATR-VAGECAARWRG---------- 216
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2PPQ_A      184 DLPAGVIHADLFQDNVFFL--------------GDELsGLIDFYFACNDllaydvsicLn----A-WCFEKDGAYNVTKG 244
1ZYL_A      192 DFTVLRLHGDCHAGNILWR--------------DGPM--FVDLDDARNGpavqdlwmlL-----N-GDKAEQRMQLETII 249
gi 76363316 192 DFATLRLHGDCHAGNILWR--------------DGPL--FVDLDDARNGpaiqdlwmlL-----N-GDKAEQRMQLETII 249
gi 81442334 203 --QLIRLHGDLHPGNLMHRd-------------EVYH--VVDLDDCRMGpavqdlwmmLa----G-NREERLGQLAELID 260
gi 81541896 191 -YQPIRLHGDCHPGNLLCRd-------------DVYH--MVDLDDCRMGpalqdlwmmL-----A-GERHERLAQIAELV 248
gi 81656586 196 -VAQLRLHGDCHVGNLLWTd------------aGPH---FVDFDDSRMGpavqdlwmlL-----S-GERAEQSRQLGEVL 253
gi 81506458 203 EVAMLRLHGDCHASNVLWIdeadalkndplrsaGPH---FVDFDDSRTGpavqdlwmlLsgdraS--MRHQLASVLAGYE 277
gi 12230805 233 TPYWTICWGDPHSGNVHFTa------------eDQMM--LFDFDQCGMGwrafdiakfLq----VsMQSGLGRNIRDAFL 294
gi 12230798 198 --NYGLVHADLHHGNFNWD--------------HGKI-TAFDFDDIGYNwfvndisilL-----Y-NVLWYPVVPYDDKA 254
gi 267043   217 -ERPALTHGDPSLANVLFT--------------KGVD--IIDWGGAMWApperdwaalQr----V-FG--TAPRSRPEFL 272
                       330
                ....*....|....*..
2PPQ_A      245 KALl----EGYQSVRPL 257
1ZYL_A      250 EAYeefseFDTAEIGLI 266
gi 76363316 250 EAYeevseFDTaeigli 266
gi 81442334 261 GYNef-hdFDPRELALI 276
gi 81541896 249 DGYnefhdFDPRQLPLL 265
gi 81656586 254 AGYedfceFDLRELNLL 270
gi 81506458 278 DFCaf-dtRELHLVEAL 293
gi 12230805 295 SGYn--------SIAPL 303
gi 12230798 255 AFTe---eFMTHFMKGY 268
gi 267043   273 RFYel--rWHLAEIAEY 287
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