1YT3,3SAF,5C0Y,1KFS


Conserved Protein Domain Family
DNA_pol_A_exo1

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pfam01612: DNA_pol_A_exo1 
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3'-5' exonuclease
This domain is responsible for the 3'-5' exonuclease proofreading activity of E. coli DNA polymerase I (polI) and other enzymes, it catalyzes the hydrolysis of unpaired or mismatched nucleotides. This domain consists of the amino-terminal half of the Klenow fragment in E. coli polI it is also found in the Werner syndrome helicase (WRN), focus forming activity 1 protein (FFA-1) and ribonuclease D (RNase D). Werner syndrome is a human genetic disorder causing premature aging; the WRN protein has helicase activity in the 3'-5' direction. The FFA-1 protein is required for formation of a replication foci and also has helicase activity; it is a homolog of the WRN protein. RNase D is a 3'-5' exonuclease involved in tRNA processing. Also found in this family is the autoantigen PM/Scl thought to be involved in polymyositis-scleroderma overlap syndrome.
Statistics
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Aligned: 29 rows
Threshold Bit Score: 120.087
Threshold Setting Gi: 461959
Created: 30-Apr-2019
Updated: 18-Jul-2019
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1YT3_A          3 YQMITTDDALASLCEAVRAFP----AIALDTEFVRTRT--YYPQLGLIQLFDG--EHLALIDPLGITD----------WS 64   Escherichia c...
O67779          3 FEYVTGEEGLKKAIKRLENSP----YLYLDTETTGDRI-------RLVQIGDE--ENTYVIDLYEIQD----------IE 59   Aquifex aeoli...
Q05254         18 VETPEDLEGFRNFIRAHLN------CLAVDTETTGLDIysDTFECRLVQFGTQ--DEAWV-VPVELGDvfied-vriaIG 87   Mycobacterium...
O51498        321 YRSITTKIELDDLIESLKKAK----YISIDTETSSLDTytAKLIGISISFKEF--EGYYIPIEAKGKIyiek---nyiIQ 391  Borreliella b...
P19822        137 YRPITDPDEAEEYIKMVYNMVi--gPVAFDSETSALYCrdGYLLGVSISHQEY--QGVYIDSDCLTEVa---------VY 203  Escherichia v...
jgi:Rmar_1349 333 YRIVRNRQQLDELVARLDGLE----RLAIDTETTSTEAmwASLVGIAFSWEKG--QGYYVPTPLPDGTpte-----tvLE 401  Rhodothermus ...
P43741        334 YETLLTQADLTRWIEKLNAAK----LIAVDTETDSLDYmsANLVGISFALENG--EAAYLPLQLDYLDapktlekstaLA 407  Haemophilus i...
P00582        330 YVTILDEETLKAWIAKLEKAP----VFAFDTETDSLDNisANLVGLSFAIEPG--VAAYIPVAHDYLDapdqisreraLE 403  Escherichia c...
O08307        339 VTIVRDATALADLVSRLRNAP----AFAFDTECTSLQPvaSDLVGISIAIAPD--TVCYIPVGHQSETqvpc---gevVT 409  Chloroflexus ...
Q55971        372 PQIITTIAQLEALVEELKKHTdadfPVAWDTETDSLDPlvANLVGIGCAWGQEpnQVAYIPLKHHQGEqlsl---giiKD 448  Synechocystis...
1YT3_A         65 PLKAILRDPSITKFLHAGSEDLEVFLNV------FGELPQPLIDT-QILAAFCGRPMS----------WGFASMVEEYSG 127  Escherichia c...
O67779         60 PLRKLINERG--IVGHNLKFDLKYLYR-------YGIFPSATFDT-MIASYLLGYER-----------HSLNHIVSNLLG 118  Aquifex aeoli...
Q05254         88 ALKRMVL--------QNASFDLQVLDQC------FGIEMEGLWPR-VLDTQILAKLVDprpfeaggfgHSLEELIAKFIS 152  Mycobacterium...
O51498        392 KFNNLFES-NPKIIGQNYKFDYKILKNN------GFNPIPPYFDT-MIAAYLIDTNSK----------VSLDFLAEKYLM 453  Borreliella b...
P19822        204 YLQKILDSENHTIVFHNLKFDMHFYKYHlgltfdKAHKERRLHDT-MLQHYVLDERRGt---------HGLKSLAMKYTD 273  Escherichia v...
jgi:Rmar_1349 402 RLAPILRRAQ-RKVGQNLKYDLVVLAQH------GVEVPPPYFDT-MVAHYLIAPEEP----------HNLDVLARQYLR 463  Rhodothermus ...
P43741        408 AIKPILENPNIHKIGQNIKFDESIFAR-------HGIELQGVE----FDTMLLSYTLNst------grHNMDDLAKRYLG 470  Haemophilus i...
P00582        404 LLKPLLEDEKALKVGQNLKYDRGILAN-------YGIELRGIA----FDTMLESYILNsva-----grHDMDSLAERWLK 467  Escherichia c...
O08307        410 ALAPFFANPQQPKFAHNAKFDMEVLAG-------AGIKVSGLAFDtMIAAAMLGKRQ------------GLKDLAFYELK 470  Chloroflexus ...
Q55971        449 LLGEILGNAIYPKVLQNAKFDRRVLAH-------HGIELGGVVLDtMLASYVLQPEET----------HNLTDLCRRYNL 511  Synechocystis...
1YT3_A        128 ---VTLDKSESRTDWL------------ARPLTERQC--------EYAAADVWYLLPITAKLMVETE 171  Escherichia coli K-12
O67779        119 ---YSMDKSYQTSDWG------------ASVLSDAQL--------KYAANDVIVLRELFPKMRDMLN 162  Aquifex aeolicus VF5
Q05254        153 e-dQAENVKKLMAKLAaeh-------ktTKAKIWSTIdlfhpeylLYAGMDTIFTARVCKSLTPLVP 211  Mycobacterium virus L5
O51498        454 h--KNIKYEDVIQKNDn----------fANISLEMAT--------SYSSEDADITFRLFNIFTKKLK 500  Borreliella burgdorferi B31
P19822        274 mgdYDFELDKFKDDYCkahkikkedftyDLIPFDIMW--------PYAAKDTDATIRLHNFFLPKIE 332  Escherichia virus T5
jgi:Rmar_1349 464 y--QMVAITELIGSGRdqk-------smRDVSIDEVG--------PYACEDTDIALQLADVLAAELD 513  Rhodothermus marinus DSM 4252
P43741        471 h-eTIA-FESLAGKGKsql-------tfNQIPLEQAT--------EYAAEDADVTMKLQQALWLKLQ 520  Haemophilus influenzae Rd ...
P00582        468 h-kTIT-FEEIAGKGKnql-------tfNQIALEEAG--------RYAAEDADVTLQLHLKMWPDLQ 517  Escherichia coli K-12
O08307        471 lpePPTTIEDLIGRGNkqi-------sfAEVPVEQAA--------PYAAADALYTLRLTERLQRQLE 522  Chloroflexus aurantiacus J...
Q55971        512 g-lVALSYKDLGLKKDqt---------iADLPLETAG--------QYCGLDCYATYLLASKLQKELD 560  Synechocystis sp. PCC 6803...
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