1YT3


Conserved Protein Domain Family
DNA_pol_A_exo1

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pfam01612: DNA_pol_A_exo1 
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3'-5' exonuclease
This domain is responsible for the 3'-5' exonuclease proofreading activity of E. coli DNA polymerase I (polI) and other enzymes, it catalyzes the hydrolysis of unpaired or mismatched nucleotides. This domain consists of the amino-terminal half of the Klenow fragment in E. coli polI it is also found in the Werner syndrome helicase (WRN), focus forming activity 1 protein (FFA-1) and ribonuclease D (RNase D). Werner syndrome is a human genetic disorder causing premature aging; the WRN protein has helicase activity in the 3'-5' direction. The FFA-1 protein is required for formation of a replication foci and also has helicase activity; it is a homolog of the WRN protein. RNase D is a 3'-5' exonuclease involved in tRNA processing. Also found in this family is the autoantigen PM/Scl thought to be involved in polymyositis-scleroderma overlap syndrome.
Statistics
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PSSM-Id: 334610
View PSSM: pfam01612
Aligned: 26 rows
Threshold Bit Score: 122.403
Threshold Setting Gi: 461959
Created: 19-Mar-2017
Updated: 23-May-2017
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1YT3_A         3 YQMITTDDALASLCEAVRAFP----AIALDTEFVRTRT--YYPQLGLIQLFDG--EHLALIDPLGITD----------WS 64   Escherichia coli
Q05254        18 VETPEDLEGFRNFIRAHLN------CLAVDTETTGLDIysDTFECRLVQFGTQ--DEAWV-VPVELGDvfied-vriaIG 87   Mycobacterium ...
O51498       321 YRSITTKIELDDLIESLKKAK----YISIDTETSSLDTytAKLIGISISFKEF--EGYYIPIEAKGKIyiek---nyiIQ 391  Borrelia burgd...
P19822       137 YRPITDPDEAEEYIKMVYNMVi--gPVAFDSETSALYCrdGYLLGVSISHQEY--QGVYIDSDCLTEVa---------VY 203  Escherichia vi...
P43741       334 YETLLTQADLTRWIEKLNAAK----LIAVDTETDSLDYmsANLVGISFALENG--EAAYLPLQLDYLDapktlekstaLA 407  Haemophilus in...
P00582       330 YVTILDEETLKAWIAKLEKAP----VFAFDTETDSLDNisANLVGLSFAIEPG--VAAYIPVAHDYLDapdqisreraLE 403  Escherichia co...
O08307       339 VTIVRDATALADLVSRLRNAP----AFAFDTECTSLQPvaSDLVGISIAIAPD--TVCYIPVGHQSETqvpc---gevVT 409  Chloroflexus a...
Q55971       372 PQIITTIAQLEALVEELKKHTdadfPVAWDTETDSLDPlvANLVGIGCAWGQEpnQVAYIPLKHHQGEqlsl---giiKD 448  Synechocystis ...
WP_012843849 333 YRIVRNRQQLDELVARLDGLE----RLAIDTETTSTEAmwASLVGIAFSWEKG--QGYYVPTPLPDGTpte-----tvLE 401  Rhodothermus m...
EDX08421     531 iANVKQSVFVTDIILKYAAKNes-iVVSLDCEGINLGL---KGEITLIEIGTTr-GEAFLFDVQSCPAmv-------tDG 598  Drosophila sim...
1YT3_A        65 PLKAILRDPSITKFLHAGSEDLEVFLNV------FGELPQPLIDT-QILAAFCGRPMS----------WGFASMVEEYSG 127  Escherichia coli
Q05254        88 ALKRMVL--------QNASFDLQVLDQC------FGIEMEGLWPR-VLDTQILAKLVDprpfeaggfgHSLEELIAKFIS 152  Mycobacterium ...
O51498       392 KFNNLFES-NPKIIGQNYKFDYKILKNN------GFNPIPPYFDT-MIAAYLIDTNSK----------VSLDFLAEKYLM 453  Borrelia burgd...
P19822       204 YLQKILDSENHTIVFHNLKFDMHFYKYHlgltfdKAHKERRLHDT-MLQHYVLDERRGt---------HGLKSLAMKYTD 273  Escherichia vi...
P43741       408 AIKPILENPNIHKIGQNIKFDESIFAR-------HGIELQGVE----FDTMLLSYTLNst------grHNMDDLAKRYLG 470  Haemophilus in...
P00582       404 LLKPLLEDEKALKVGQNLKYDRGILAN-------YGIELRGIA----FDTMLESYILNsva-----grHDMDSLAERWLK 467  Escherichia co...
O08307       410 ALAPFFANPQQPKFAHNAKFDMEVLAG-------AGIKVSGLAFDtMIAAAMLGKRQ------------GLKDLAFYELK 470  Chloroflexus a...
Q55971       449 LLGEILGNAIYPKVLQNAKFDRRVLAH-------HGIELGGVVLDtMLASYVLQPEET----------HNLTDLCRRYNL 511  Synechocystis ...
WP_012843849 402 RLAPILRRAQ-RKVGQNLKYDLVVLAQH------GVEVPPPYFDT-MVAHYLIAPEEP----------HNLDVLARQYLR 463  Rhodothermus m...
EDX08421     599 GLKTVLEHDQVIKVIHDCRNDAANLYLQ------FGILLRNVFDT-QAAHAILQYQESgkqvy-kakyISLNSLCEQYNA 670  Drosophila sim...
1YT3_A       128 ---VTLDKSESRTDWL------------ARPLTERQC--------EYAAADVWYLLP--ITAKLMVETE 171  Escherichia coli
Q05254       153 e-dQAENVKKLMAKLAaeh-------ktTKAKIWSTIdlfhpeylLYAGMDTIFTAR--VCKSLTPLVP 211  Mycobacterium phage L5
O51498       454 h--KNIKYEDVIQKNDn----------fANISLEMAT--------SYSSEDADITFR--LFNIFTKKLK 500  Borrelia burgdorferi B31
P19822       274 mgdYDFELDKFKDDYCkahkikkedftyDLIPFDIMW--------PYAAKDTDATIR--LHNFFLPKIE 332  Escherichia virus T5
P43741       471 h-eTIA-FESLAGKGKsql-------tfNQIPLEQAT--------EYAAEDADVTMK--LQQALWLKLQ 520  Haemophilus influenzae Rd...
P00582       468 h-kTIT-FEEIAGKGKnql-------tfNQIALEEAG--------RYAAEDADVTLQ--LHLKMWPDLQ 517  Escherichia coli K-12
O08307       471 lpePPTTIEDLIGRGNkqi-------sfAEVPVEQAA--------PYAAADALYTLR--LTERLQRQLE 522  Chloroflexus aurantiacus ...
Q55971       512 g-lVALSYKDLGLKKDqt---------iADLPLETAG--------QYCGLDCYATYL--LASKLQKELD 560  Synechocystis sp. PCC 680...
WP_012843849 464 y--QMVAITELIGSGRdqk-------smRDVSIDEVG--------PYACEDTDIALQ--LADVLAAELD 513  Rhodothermus marinus
EDX08421     671 pcnPIKDQLKQIYRRDqkf-------waKRPLTREMM--------LYAAGDVLVLIHdqLFGNLARQIk 724  Drosophila simulans
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