6EI9,3W9Z


Conserved Protein Domain Family
Dus

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pfam01207: Dus 
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Dihydrouridine synthase (Dus)
Members of this family catalyze the reduction of the 5,6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes, and also in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown, but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities, which may overlap. Dus 1 from Saccharomyces cerevisiae acts on pre-tRNA-Phe, while Dus 2 acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit, requiring NADPH or NADH, and is stimulated by the presence of FAD. Some family members may be targeted to the mitochondria and even have a role in mitochondria.
Statistics
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Aligned: 18 rows
Threshold Bit Score: 319.645
Threshold Setting Gi: 1730664
Created: 24-Apr-2019
Updated: 18-Jul-2019
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6EI9_A  32 IAA-PMAGITDRPFRTLCYEMGAG--------------------------LTVSEMMSSNPQVWESDKSRLRMVHIDEP- 83  Escherichia coli
Q50049   1 MLA-PMAGVTNVTFRTLCREFGSTrgnllrpapphlrspleqsqvgtisgLYVCEMVTARALVERNAVTIHMTTFAPDE- 78  Mycobacterium leprae TN
P37567  14 VLA-PMAGVCNSAFRLTVKEFGAG--------------------------LVCAEMVSDKAILYNNARTMGMLYIDERE- 65  Bacillus subtilis sub...
P33371   4 LLA-PMEGVLDSLVRELLTEVNDYd-------------------------LCITEFVRVVDQLLPVKVFHRICPELQNAs 57  Escherichia coli K-12
P44606   4 ILA-PMQGVLDPFVRELLTEVNDYd-------------------------LCITEFVRVVDQLLPEKVFYRLCPELKNQg 57  Haemophilus influenza...
P72872  14 SVA-PMMDHTDRHFRYFLRQLTRHt-------------------------LLYTEMITAQAILHGDRQRLLNFSPEEKP- 66  Synechocystis sp. PCC...
P44794  14 SVA-PMLDWTTRHCRYFHRQFSKHa-------------------------LLYTEMVTAPAIIHA-KYDHLDFDLQENP- 65  Haemophilus influenza...
P32695  29 sVA-PMLDWTDRHCRYFLRLLSRNt-------------------------LLYTEMVTTGAIIHG-KGDYLAYSEEEHP- 80  Escherichia coli K-12
P53720  31 DLV-WSPEIIDKKLIQCVRKENTAl-------------------------QTVDYVVPSKVQTRPETLVFRTYPKLESS- 83  Saccharomyces cerevis...
P53759  31 RIVaPMVDQSELAWRILSRRYGAT--------------------------LAYTPMLHAKLFATSKKYREDNWSSLDGSs 84  Saccharomyces cerevis...
6EI9_A  84 -----GIRTVQIAGSDPKEMADAARINVEs-GAQIIDINMGCPAKKVNRKLAGSALLQYPDVVKSILTEVVNAVD---VP 154 Escherichia coli
Q50049  79 -----SPRSLQLYTVDPATTYTAAKMVADegLADHIDMNFGCPMPKVTRRGGGAALPYKRRLFGQIVAAAVRATEgtkIP 153 Mycobacterium leprae TN
P37567  66 -----KPLSLQIFGGKKETLVEAAKFVDQntTADIIDINMGCPVPKITKCDAGAKWLLDPDKIYEMVSAVVDAVN---KP 137 Bacillus subtilis sub...
P33371  58 rtpsgTLVRVQLLGQFPQWLAENAARAVEl-GSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPa-hLP 135 Escherichia coli K-12
P44606  58 ftssgTPVRVQLLGQHPKCLAENAIRAIDl-GSHGIDLNCGCPSKTVNGSNGGAALLKQPELIYRATQALRRAVPs-eFP 135 Haemophilus influenza...
P72872  67 -------VALQLGGDDPQLLAECARIGQDw-GYDEINLNVGCPSDRVQSGNFGACLMAQPDLVAQCVSAMQKAVE---IP 135 Synechocystis sp. PCC...
P44794  66 -------VALQLGGSDPEQLKYCAKLAEEr-GYHEINLNVGCPSDRVQNGMFGACLMAKADLVADCVEQMQSAVK---IP 134 Haemophilus influenza...
P32695  81 -------VALQLGGSDPAALAQCAKLAEAr-GYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVS---IP 149 Escherichia coli K-12
P53720  84 ------KLIFQIGSASPALATQAALKVIN--DVSGIDINAGCPKHFSIHSGMGSALLRTPDTLCLILKELVKNVG---NP 152 Saccharomyces cerevis...
P53759  85 v---dRPLVVQFCANDPEYLLAAAKLVED--KCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLIHNLINTLHKNLK---VP 156 Saccharomyces cerevis...
6EI9_A 155 VTLKIRTGWAp--eHRNCEEIAQLAEDCG-IQALTIHGR-------TRACLFNG--EAEYDSIRAVKQKVS--IPVIANG 220 Escherichia coli
Q50049 154 VTVKFRIGIDd--eHHTHLDAGRIAEAEG-AAAVALHAR-------TAAQRYSG--TADWAQIAQLKQQVRt-IPVLGNG 220 Mycobacterium leprae TN
P37567 138 VTVKMRMGWDe--dHIFAVKNAQAVERAG-GKAVALHGR-------TRVQMYEG--TANWDIIKEVKQSVS--IPVIGNG 203 Bacillus subtilis sub...
P33371 136 VSVKVRLGWDs--gEK-KFEIADAVQQAG-ATELVVHGR-------TKEQGYRAe-HIDWQAIGDIRQRLN--IPVIANG 201 Escherichia coli K-12
P44606 136 VSVKVRLGWDd--iSQ-AFEIADAVEQGG-ATEITVHGR-------TKADGYRAd-RINWKKISEVRERLS--IPVIANG 201 Haemophilus influenza...
P72872 136 VTVKHRIGIDhrdsYEDLVHFVEIVANAG-CQRFTVHARkawlqglSPKENRTIp-PLRYEDVYQLKKDFPq-LLIEING 212 Synechocystis sp. PCC...
P44794 135 VTVKTRIGIDeldsYEFLCDFIEKVQGKG-CQEFIIHARkawlsglSPKENREIp-PLDYIRVYQLKRDFPh-LTIAING 211 Haemophilus influenza...
P32695 150 VTVKTRIGIDdqdsYEFLCDFINTVSGKGeCEMFIIHARkawlsglSPKENREIp-PLDYPRVYQLKRDFPh-LTMSING 227 Escherichia coli K-12
P53720 153 HSKPISVKIRlldtKQDTLQLVKRLCATG-ITNLTVHCRk------TEMRNREQpiTDYIAEIYEICQANN--VSLIVNG 223 Saccharomyces cerevis...
P53759 157 VTAKIRIFDD----CEKSLNYAKMVLDAG-AQFLTVHGR-------VREQKGQKtgLANWETIKYLRDNLPkeTVFFANG 224 Saccharomyces cerevis...
6EI9_A 221 DITDP---LKARAVLDYTGADALMIGRAAQGRPWIFREiQhYLDTGELLPPLP-LAEV----KRLLCAHVRELHDFYGPA 292 Escherichia coli
Q50049 221 DIYDA---SDALAMMAVTGCDGVVIGRGCLGRPWLFAElS-AAFTGRPAPAPPtLGEV----AEIVRRHGKLLVAHFGED 292 Mycobacterium leprae TN
P37567 204 DVKTP---QDAKRMLDETGVDGVMIGRAALGNPWMIYRtVqYLETGKLKEEPQ-VREK----MAVCKLHLDRLIDLKGEN 275 Bacillus subtilis sub...
P33371 202 EIWDW---QSAQQCMAISGCDAVMIGRGALNIPNLS-----RVVK-YNEPRMP--WPE----VVALLQKYTRLEKQG--D 264 Escherichia coli K-12
P44606 202 EIWHW---QDGQDCLSQTGCQDLMVGRGALNIPNLS-----HVLK-SNAEKMP--WYE----IQKILQKYANVENEY--D 264 Haemophilus influenza...
P72872 213 GITQT---EQIQQHLSH--VDAVMVGRAAYENPYLFA----TVDRDIYHKTNL-VPSR----AEIIERMLPYVEERLRHG 278 Synechocystis sp. PCC...
P44794 212 GIKTI---EEMKQHLQY--VDGVMVGREAYQNPSLLG----QIDQALFDPNAP-IVTA----HEAVESMLPYIEQQLSQG 277 Haemophilus influenza...
P32695 228 GIKSL---EEAKAHLQH--MDGVMVGREAYQNPGILA----AVDREIFGSSDT-DADP----VAVVRAMYPYIERELSQG 293 Escherichia coli K-12
P53720 224 AIRDRshfHDLQANHWKNTNIGGMIAECAERDPTVFDh---TSKPSEDGPSWVvACREfiqwATKFDNHIGNTKYMLSRI 300 Saccharomyces cerevis...
P53759 225 NILYP---EDISRCMEHIGADAVMSAEGNLYNPGVFNVgQtKNKEKIFPRVDKiIREYfqivKECQESKASKTAMKSHFF 301 Saccharomyces cerevis...
6EI9_A 293 KGYRIARKHVSWYLQE---HAPNDQFRRTFNAIEDASEQ---LEALE--AYFENFA 340 Escherichia coli
Q50049 293 KGMRDIRKHIAWYLHG---FPAGSELRNALALVKTLDELdclLNRLDgaVPFPDAA 345 Mycobacterium leprae TN
P37567 276 VAVREMRKHAAWYLKG---VKGNANVRNEINHCETREEF---VQLLD---AFTVEV 322 Bacillus subtilis subsp. subtilis str. 168
P33371 265 TGLYHVARIKQWLSY----LRKE--Y-DEATELFQHVRV---LNNSP---DIARAI 307 Escherichia coli K-12
P44606 265 SGFYHVARIKQWLRY----LNKE--Y-NEANQVFDKIKT---CQTAE---DLKLRL 307 Haemophilus influenzae Rd KW20
P72872 279 DRLNQITRHLLSLFNG---QPRAKFWRRTLSDSTLLASA---GPELL--QTALQAQ 326 Synechocystis sp. PCC 6803 substr. Kazusa
P44794 278 IHLNHIVRHILGAFQN---CKGARQWRRYLSENAFKQGA---GIEVV--ETALSFV 325 Haemophilus influenzae Rd KW20
P32695 294 TYLGHITRHMLGLFQG---IPGARQWRRYLSENAHKAGA---DINVL--EHALKLv 341 Escherichia coli K-12
P53720 301 VPGKSVFFQYFARCKSp--EEVSFVLKQLNDDGSAQTDPseyLENCRaqEKALKNA 354 Saccharomyces cerevisiae S288C
P53759 302 KILRPFLPHHTDIRSTlatMNAKATWEEWEEQVVKPVEKvvqEIFEQpdIAIKDEI 357 Saccharomyces cerevisiae S288C
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