3E4E,3RUK,5T6Q,3N9Y,3MZS


Conserved Protein Domain Family
p450

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pfam00067: p450 
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Cytochrome P450
Cytochrome P450s are haem-thiolate proteins involved in the oxidative degradation of various compounds. They are particularly well known for their role in the degradation of environmental toxins and mutagens. They can be divided into 4 classes, according to the method by which electrons from NAD(P)H are delivered to the catalytic site. Sequence conservation is relatively low within the family - there are only 3 absolutely conserved residues - but their general topography and structural fold are highly conserved. The conserved core is composed of a coil termed the 'meander', a four-helix bundle, helices J and K, and two sets of beta-sheets. These constitute the haem-binding loop (with an absolutely conserved cysteine that serves as the 5th ligand for the haem iron), the proton-transfer groove and the absolutely conserved EXXR motif in helix K. While prokaryotic P450s are soluble proteins, most eukaryotic P450s are associated with microsomal membranes. their general enzymatic function is to catalyze regiospecific and stereospecific oxidation of non-activated hydrocarbons at physiological temperatures.
Statistics
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PSSM-Id: 333810
View PSSM: pfam00067
Aligned: 55 rows
Threshold Bit Score: 246.029
Threshold Setting Gi: 117304
Created: 13-Mar-2017
Updated: 23-May-2017
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3E4E_A        12 PPGPFPLPIIGNLFQLeL-K-NIPKS-FTRLAQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKdefSGrgDLPAFH-- 86
gi 399288     61 PRLGQLRFFFQLFVQG-Y-A-LQLHQlQVLYKAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGkypVRndMELWKEhr 137
gi 341940566  42 PQYSRNKWLKMIQILReQgQ-ENLHLeMHQVFRELGPIFRHSVGKTQIVSVMLPEDAEKLHQVESmlpRRmhLEPWVAhr 120
gi 117263     42 PRCPGNKWMRMLQIWKeQsS-ENMHLdMHQTFQELGPIFRYDVGGRHMVFVMLPEDVERLQQADShhpQRmiLEPWLAyr 120
gi 584999     50 PGLWRNGLANLYSFWKlDgF-RNIHRvMVHNFNTFGPIYREKIGYYDSVNIIKPEMPAILFKAEGhypKRltVEAWTSyr 128
gi 143811381  52 PSPGDNGWLNLYHFWReTgT-HKVHLhHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGpnpERflIPPWVAyh 130
gi 117259     52 PSPGDNGWLNLYHFWReKgS-QRIHFrHIENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGsypERydIPPWLAyh 130
gi 117304     12 PKFQSRAEEFFPIQWYk----EMLNNsPVYFHEET-NTWNVFQYEHVKQVLSNYDFFSSDGQRTTi-fVG--DNSKKKs- 82
3N9Y_A        12 PSPGDNGWLNLYHFWReT-GtHKVHLhHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGpnpERflIPPWVAyh 90
3MZS_A        14 PSPGDNGWLNLYHFWReK-GsQRIHFrHIENFQKYGPIYREKLGNLESVYIIHPEDVAHLFKFEGsypERydIPPWLAyh 92
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3E4E_A        87 --AHRDRGIIFNNGPTWKDIRRFSLTTLRNYgmgK-Qg-NESRIQREAHFLLEALRKTQGQP------F----DPTFLIG 152
gi 399288    138 dqHDLTYGPFTTEGHHWYQLRQALNQRLLKPaeaA-L--YTDAFNEVIDDFMTRLDQLRAESasgnqvS----DMAQLFY 210
gi 341940566 121 elRGLRRGVFLLNGPEWRLNRLRLNRNVLSPk--A-VqkFVPMVDMVARDFLETLKEKVLQNargsltM----DVQQSLF 193
gi 117263    121 qaRGHKCGVFLLNGPQWRLDRLRLNPDVLSLp--A-LqkYTPLVDGVARDFSQTLKARVLQNargsltL----DIAPSVF 193
gi 584999    129 dyRNRKYGVLLKNGEDWRSNRVILNREVISPk--V-LgnFVPLLDEVGQDFVARVHKKIERSgqdkwtT----DLSQELF 201
gi 143811381 131 qyYQRPIGVLLKKSAAWKKDRVALNQEVMAPe--A-TknFLPLLDAVSRDFVSVLHRRIKKAgsgnysG----DISDDLF 203
gi 117259    131 ryYQKPIGVLFKKSGTWKKDRVVLNTEVMAPe--A-IknFIPLLNPVSQDFVSLLHKRIKQQgsgkfvG----DIKEDLF 203
gi 117304     83 --TSPITNLTNLDPPDHRKARSLLAAAFTPR---S-LknWEPRIKQIAADLVEAIQKNSTIN------------IVDDLS 144
3N9Y_A        91 qyYQRPIGVLLKKSAAWKKDRVALNQEVMAPe--AtKn-FLPLLDAVSRDFVSVLHRRIKKAg----sGnysgDISDDLF 163
3MZS_A        93 ryYQKPIGVLFKKSGTWKKDRVVLNTEVMAPe-aI-Kn-FIPLLNPVSQDFVSLLHKRIKQQgsgkfvG----DIKEDLF 165
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3E4E_A       153 CAPCNVIADILFRKHFD-YNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPs--FLhYLPGSHRKVIKNV-AEVKEYVSER 228
gi 399288    211 YFALEAICYILFEKRIGcLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWtrpVL-PFWKRYLDGWNAIfSFGKKLIDEK 289
gi 341940566 194 NYTIEASNFALFGERLGlLGHDLSPGSLKFIHALHSMFKSTSQLLFLPKSl--TR-WTSTRVWKEHFDAwDVISEYANRC 270
gi 117263    194 RYTIEASTLVLYGERLGlLTQQPNPDSLNFIHALEAMLKSTVQLMFVPRRl--SR-WMSTNMWREHFEAwDYIFQYANRA 270
gi 584999    202 KYALESVGSVLYGERLGlMLDYINPEAQHFIDCISLMFKTTSPMLYIPPAm--LR-RVGAKIWRDHVEAwDGIFNQADRC 278
gi 143811381 204 RFAFESITNVIFGERQGmLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDl--FR-LFRTKTWKDHVAAwDVIFSKADIY 280
gi 117259    204 HFAFESITNVMFGERLGmLEETVNPEAQKFIDAVYKMFHTSVPLLNVPPEl--YR-LFRTKTWRDHVAAwDTIFNKAEKY 280
gi 117304    145 SPFPSLVIADLFGVP---VKDR--YQFKKWVDILF--------QPYDQER--------LEEIEQEKQRAgAEYFQYLYPI 203
3N9Y_A       164 RFAFESITNVIFGERQGmLEEVVNPEAQRFIDAIYQMFHTSVPMLNLPPDl--FR-LFRTKTWKDHVAAwDVIFSKADIY 240
3MZS_A       166 HFAFESITNVMFGERLGmLEETVNPEAQKFIDAVYKMFHTSVPLLNVPPEl--YR-LFRTKTWRDHVAAwDTIFNKAEKY 242
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3E4E_A       229 VKEHHQSLD-P----NCPRDLTDCLLVEmekekhSaerLYTMDGITVTVADLFFAGTETTSTTLRYGLL-ILMKYPEIEE 302
gi 399288    290 LEDMEAQLQ-AagpdGIQVSGYLHFLLAs-----G---QLSPREAMGSLPELLMAGVDTTSNTLTWALY-HLSKDPEIQE 359
gi 341940566 271 IWKVHQELR-Lg--sSQTYSGIVAELISq--------gSLPLDAIKANSMELTAGSVDTTAIPLVMTLF-ELARNPDVQK 338
gi 117263    271 IQRIYQELA-Lg--hPWHYSGIVAELLMr--------aDMTLDTIKANTIDLTAGSVDTTAFPLLMTLF-ELARNPEVQQ 338
gi 584999    279 IQNIYRTMR-QdtntHGKYPGVLASLLMl--------dKLSIEDIKASVTELMAGGVDTTSITLLWTLY-ELARHPDLQE 348
gi 143811381 281 TQNFYWELR-QkgsvHHDYRGILYRLLGd--------sKMSFEDIKANVTEMLAGGVDTTSMTLQWHLY-EMARNLKVQD 350
gi 117259    281 TEIFYQDLR-Rkt-eFRNYPGILYCLLKs--------eKMLLEDVKANITEMLAGGVNTTSMTLQWHLY-EMARSLNVQE 349
gi 117304    204 VIEKRSNLS-Dd----IISDLIQAEVDGe---------TFTDEEIVHATMLLLGAGVETTSHAIANMFYsFLYDDKSLYS 269
3N9Y_A       241 TQNFYWELR-QkgsvHHDYRGILYRLLGd-----S---KMSFEDIKANVTEMLAGGVDTTSMTLQWHLY-EMARNLKVQD 310
3MZS_A       243 TEIFYQDLRrKt--eFRNYPGILYCLLKs-----E---KMLLEDVKANITEMLAGGVNTTSMTLQWHLY-EMARSLNVQE 311
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3E4E_A       303 KLHEEIDRVI-GPsRIPAIKDRQEMPYMDAVVHEIQRFITLVPsNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQE 381
gi 399288    360 ALHEEVVGVVpAG-QVPQHKDFAHMPLLKAVLKETLRLYPVVP-TNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTA 437
gi 341940566 339 ALRQESLAAE-ASiaANPQKAMSDLPLLRAALKETLRLYPVGG-FLERILSSDLVLQNYHVPAGTLVLLYLYSMGRNPAV 416
gi 117263    339 AVRQESLVAE-ARisENPQRAITELPLLRAALKETLRLYPVGItLE-REVSSDLVLQNYHIPAGTLVKVLLYSLGRNPAV 416
gi 584999    349 ELRAEVAVAR-QStqGDMLQMLKMIPLVKGALKETLRLHPVAVsLQ-RYITEEIVIQNYHIPCGTLVQLGLYAMGRDPDV 426
gi 143811381 351 MLRAEVLAAR-HQaqGDMATMLQLVPLLKASIKETLRLHPISV-TLQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTF 428
gi 117259    350 MLREEVLNAR-RQaeGDISKMLQMVPLLKASIKETLRLHPISVtLQ-RYPESDLVLQDYLIPAKTLVQVAIYAMGRDPAF 427
gi 117304    270 ELRNN-------------------RELAPKAVEEMLRYRFHIS-RRDRTVKQDNELLGVKLKKGDVVIAWMSACNMDETM 329
3N9Y_A       311 MLRAEVLAAR-HQaQGDMATMLQLVPLLKASIKETLRLHPISVt-LQRYLVNDLVLRDYMIPAKTLVQVAIYALGREPTF 388
3MZS_A       312 MLREEVLNAR-RQaEGDISKMLQMVPLLKASIKETLRLHPISVt-LQRYPESDLVLQDYLIPAKTLVQVAIYAMGRDPAF 389
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3E4E_A       382 FPDPEKFKPEHFLNENG---KF-KYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLK--PLVDPKDIDLSpihi 455
gi 399288    438 FSEPESFQPHRWLRNSQpatPRiQHPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVlaPETGELKSVARi--- 514
gi 341940566 417 FPRPERYMPQRWLERKR--------SFQHLAFGFGVRQCLGRRLAEVEMMLLLHHILKTFQVEtlRQEDVQMAYRF---- 484
gi 117263    417 FARPESYHPQRWLDRQG----S-GSRFPHLAFGFGVRQCLGRRVAEVEMLLLLHHVLKNFLVEtlEQEDIKMVYRF---- 487
gi 584999    427 FPRPEKYLPSRWLRTEN-------QYFRSLGFGFGPRQCLGRRIAETEMQLFLIHMLENFRVDkqRQVEVHSTFEL---- 495
gi 143811381 429 FFDPENFDPTRWLSKDK----N-ITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEiqHLSDVGTTFNL---- 499
gi 117259    428 FSSPDKFDPTRWLSKDK----D-LIHFRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVEmqHIGDVDTIFNL---- 498
gi 117304    330 FENPFSVDIHRPTNKK------------HLTFGNGPHFCLGAPLARLEMKIILEAFLEAFSHIe-PFEDFELEPHLt--- 393
3N9Y_A       389 FFDPENFDPTRWLSKDK---N--ITYFRNLGFGWGVRQCLGRRIAELEMTIFLINMLENFRVEiqHLSDVGTTFNL---- 459
3MZS_A       390 FSSPDKFDPTRWLSKDK---DL-IH-FRNLGFGWGVRQCVGRRIAELEMTLFLIHILENFKVEmqHIGDVDTIFNL---- 460
                        490
                 ....*....|...
3E4E_A       456 gFGCIPPRYKLCV 468
gi 399288    515 -VLVPNKKVGLQF 526
gi 341940566 485 -VLMPSSSPVLTF 496
gi 117263    488 -ILMPSTLPLFTF 499
gi 584999    496 -ILLPEKPILLTL 507
gi 143811381 500 -ILMPEKPISFTF 511
gi 117259    499 -ILTPDKPIFLVF 510
gi 117304    394 aSATGQSLTYLPM 406
3N9Y_A       460 -ILMPEKPISFTF 471
3MZS_A       461 -ILTPDKPIFLVF 472
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