Conserved Protein Domain Family
AmyAc_bac_euk_BE

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cd11321: AmyAc_bac_euk_BE 
Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes
Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
Statistics
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PSSM-Id: 200460
Aligned: 34 rows
Threshold Bit Score: 652.375
Created: 24-May-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitecatalytic site
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
CAB91480     182 QSERYTFKHKRPSKPEs--lRIYEAHVGISSPEC-----KVATYKEFTKTMLPRIKNLGYNAIQLMAIMEHAYYASFGYQ 254 Neurospora crassa
AAQ66793     148 PDTPYEMKHPHPNRKDep-lLIYECHIGMSSEEE-----KVSTYEEFRRNILPRICKDGYNAIQLMAIQEHPYYASFGYH 221 Porphyromonas g...
YP_002603621 159 PESPYMWRHSSPDPADl---FIYETHVGMAQETW-----GVGTFKEFETRVLPRIKTAGYTAIQIMAVQEHPYYGSFGYH 230 Desulfobacteriu...
ZP_07357159  174 PGQPYRFRHRRPGAPVf--pRIYEAHVGMAQSSLthhgeSVGSYDEFSGSVLPRIKADGYTAVQLMGILEHPLYRSFGYQ 251 Desulfovibrio s...
ZP_06241263  155 PEQPYVFRHASPPTPAa--pLIYESHVGMAQEEP-----KVGSFDEYREKILPKIAASGYNTIQLMAVMGHPYYGSFGYH 227 Victivallis vad...
ABJ71766     143 TQPKYEWKNKSPKLSEa--pLIYEAHIGISTEEY-----KINSYKEFTRDVLPRIKKDGYNTIQLMAIMEHPLYASFGYQ 215 Lactococcus lac...
ZP_02870900  151 PKDQYIWRYKTPPLPEt--pLIYEAHIGMASTDE-----KVASYSEFTKNVLPRIKDLGYNTVQLMAIAEHPYYGSFGYH 223 candidate divis...
CAJ69413     151 PKTPFIWTDNNFDLKNitspLIYECHIGMSTESE-----SIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPYYASFGYQ 225 Clostridium dif...
YP_001878350 154 PEHPYEWRNNGFDPSRvevpFVYEAHVGMGGEEG-----RVHTYREFADEVLPRIARLGYNTVQLMAIQEHPYYGSFGYH 228 Akkermansia muc...
XP_001321781 153 PEKKYVFKHPKPAPLDca-lLIYETHIGMAGVEP-----RIHTYKEFEENVLPMIKKDGYNAIQIMAVMEHPYYGSFGYQ 226 Trichomonas vag...
Feature 1                                                                                        
CAB91480     255 VNSFFAaSSRYGPPEDLKELVDTAHAMGIAVLLDVVHSHASKNvlDGLNEFDGTDHQYfhgggrgkhdLWDSRLFNYGHH 334 Neurospora crassa
AAQ66793     222 VSSFFApSSRFGTPEDLKRLIDEAHALGLYVIMDLVHSHAVKNevEGLGLYDGTHTLFfhdgnrglhpAWDSYCFDYGRD 301 Porphyromonas g...
YP_002603621 231 VTNFFAvSSRFGTPEEFKSLVDAAHGLDLKVFMDVVHSHSSSNeiEGLSRFDGSLDQFfhsgdrgihrLWDSRCFDYGKP 310 Desulfobacteriu...
ZP_07357159  252 VSSYFApSSRYGTPDQFKALVDTAHGLGLAVILDITHGHACPNteQGLARYDGSRYFFsek-----fnQWGTPSFDYSRE 326 Desulfovibrio s...
ZP_06241263  228 VANFFAiASRFGTPDQFKALVDAAHGCGLRVIIDLVHSHAVKNevEGLAKFDGTRYAYfhegsrgehsGWDSLCFNYAKP 307 Victivallis vad...
ABJ71766     216 VSNFFAiSSRFGQPEDLMELIDKAHGLGLQVLLDVVHSHAVKNieDGLNYFDGTENQYfhegergnhpAWKTKLFNYGKD 295 Lactococcus lac...
ZP_02870900  224 VANFFApSSRFGTPDDLKHLIDIAHSLGLRVIMDIVHAHSVSNenEGLGNFAGDKSQYfcagergrhsQWDSLVFDYGKP 303 candidate divis...
CAJ69413     226 VSNFYAiSSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNtlEGINEFDGSEHQFfhsgskgnhpAWGTKLFNYGKP 305 Clostridium dif...
YP_001878350 229 VSSFFApSSRFGEPEDLKYLIDQAHGLGIAVLLDVVHSHAVKNeaEGLNNFDGSGGMYflpgergrhpDWDSCCFDYGRD 308 Akkermansia muc...
XP_001321781 227 VTNFFAiSSRFGTPEELKSMIDTAHGMGIHVFLDLVHSHASKNvaEGINHFDGTDHQYfheggrgyhpLWDSRCFNYNHP 306 Trichomonas vag...
Feature 1                              # ##                                                      
CAB91480     335 EVMRFLLSNLRFWMDEYAFDGFRFDGVTSMLYLHHGIgtg----fsGGYHEYFGPDVDEEAVVYLMLANEMLHQLYPNVI 410 Neurospora crassa
AAQ66793     302 NVLHFLLSNCKYWLTEFGFDGFRFDGVTSMLYYSHGLgd-----rfDNYDSYYNGHQDGDAIAYLILANKLIHELCPDAI 376 Porphyromonas g...
YP_002603621 311 MVLNFLLSNLRYWIEEFHLDGFRFDGVTSMLFYDHGLgr-----afTCYDDYYGNSVDLDALAYLYLANRFVHAMIPNCV 385 Desulfobacteriu...
ZP_07357159  327 MTRRFLLSNCRYWLEEFRVDGFRFDAVGNMLYRDHGVdddfshvgrCFYGKDGEPRADEDGELYLCLANALTHELRPQAV 406 Desulfovibrio s...
ZP_06241263  308 EVLHFLLSNCRFYLDEYRVDGFRFDGVTSMMYLHHGLgk-----tfTGYDDYFGGDVDEDALTYLALANRVIHEVRPDAL 382 Victivallis vad...
ABJ71766     296 EVLHFLLSNLKFWLDIYHFDGFRFDGVTSMLYHDHGLgt-----afTDYSKYFSLNTDVEAVTYLMLANELTHLFNPSAT 370 Lactococcus lac...
ZP_02870900  304 EVVHFLLSNVRYWLDEFRFDGFRFDGVTSMIYSHHGLgk-----sfTSYNDYYNNTLQLDALAYLQMANDVAHSVGKSIL 378 candidate divis...
CAJ69413     306 EVIHFLLSNIKFWLNEYHFDGFRFDGVTSMLYHNHGLgv-----sfDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSI 380 Clostridium dif...
YP_001878350 309 EVIEFLLSNVRWWLEEFRFDGFRFDGVTSMLYFHRGHep------fGDLGAYFGSSVDLDAVAYLQLAATLIQRVKPGAI 382 Akkermansia muc...
XP_001321781 307 EVQRFLLSNLRYYMEEYSFDGFRFDGVTSMMYLHHGNmy-----sfNSIDCYFCDLVDREAVTYLMLANTVIHMVNPNAI 381 Trichomonas vag...
Feature 1           #                                                                   ##       
CAB91480     411 TVAEDVSGMPALCLPlsLGGVGFDYRLAMAiPDMWIKILKeKKDEEWDManitwtltNRRHGEKTIAYCESHDQALVGDK 490 Neurospora crassa
AAQ66793     377 TIAEEVSGMPGLALPlaDGGYGFDYRLAMNiPDFWIKLIKeHRDEDWNPgdiwyqltNRRQEEKTISYAESHDQALVGDK 456 Porphyromonas g...
YP_002603621 386 TIAEEVSGYPGIATSqsDGGTGFDYRYAMGiPDFWIRLLKeYRDEQWPLsllwqelnSRREDERTISYAESHDQAMVGDK 465 Desulfobacteriu...
ZP_07357159  407 TIAEEFSGMPGLTCPpqEGGLGFDYRFAMGiPDYWEKCIEaPRDMGSLWye----mtNHRPYDRTISYVECHDQCINGDD 482 Desulfovibrio s...
ZP_06241263  383 TVAEDVSGMPGLAAPasGGGIGFDCRMAMGvTDMWFKLFD-LPDQDWNMfylfheltNRRRDERSISYVESHDQAIVGGK 461 Victivallis vad...
ABJ71766     371 TIAEDMSAMPGMALPisAGGIGFDYRLSMGiPDFWIKQLKeKTDNSLDLlslwweltTRRPGEKNIGYSESHDQALVGDK 450 Lactococcus lac...
ZP_02870900  379 TIAEDTSALPGLALSgkNGGIGFDYRLSMGvPDLWIKILKeKKDEDWDLvhlfyeltARRPEEKVISYAESHDQSMVGDK 458 candidate divis...
CAJ69413     381 SIAEDMSGMPGMCIPikDGGIGFDYRLAMGvPDFWIKTISnLSDEDWDLgkmwyeltTRRPGEKNIGYCESHDQALVGDK 460 Clostridium dif...
YP_001878350 383 AIAEDMSGMPGLCRPvdEGGIGFSHRLAMGiPDYWIKLLKeKKDEEWSMgdmwhtltNRRYGEPHVAYCESHDQALVGDK 462 Akkermansia muc...
XP_001321781 382 SIAEDVSGMVGLARSieDGGMGFDYRLGMSsPDMWIKMLKeQRDEDWNVgnvafelsNRPYKEKTVAYSESHDQALVGDK 461 Trichomonas vag...
Feature 1                                                                                        
CAB91480     491 TLmmhlcDAELYTNMSIltplTPVIDRGMALHKMIRLLTHSLGGEGYLNFEGNEFGHPEWLDFPREGNQnsfWYARRQLN 570 Neurospora crassa
AAQ66793     457 TIifrliDADMYWYMNKqs-cVHSVDRGIALLKMIRLFTATTMNGGYLNFMGNEFGHPEWIDFPREGNGwsyKYARRQWS 535 Porphyromonas g...
YP_002603621 466 TLmmhlmGRAIYSCMERtn-tSITTFRAVALHKMIRLITLATAGNGYLNFMGNEFGHPEWIDFPSAANNwsyQHARRQWS 544 Desulfobacteriu...
ZP_07357159  483 AMiwrllGDDMYHYMSVgt-eSWNVSRGLAFYRLMRLITLATADAGYLNFMGNEFGHPEWLDAEAHAHRq--WYLADRPD 559 Desulfovibrio s...
ZP_06241263  462 TAifrlaDAAMYDAMHAgs-qNLAVDRAIALHKMIRLATAAAAGHGYLNFMGNEFGHPEWIDFPREGNGwslQHARRQWS 540 Victivallis vad...
ABJ71766     451 TLmmwlaNEEIYWNMDIns-qSLIIDRAIALHKLIRLITFSLAGEGYLNFMGNEFGHPEWLDFPREENHddfQHARRQWS 529 Lactococcus lac...
ZP_02870900  459 TImfrlaDKTMYWHMQKsd-nNIEIERAIALHKMIRLITASTNGGGYLNFMGNEFGHPEWIDFPREGNKwsfAHARRRWD 537 candidate divis...
CAJ69413     461 TIifwlaDKEMYWNMEIns-nNHVINRAISLIKLIKLITFSLAGEGYLNFMGNEFGHPEWIDFPREGNNwsyKYARRQWS 539 Clostridium dif...
YP_001878350 463 TLafrlmDAEMYWKMAVdq-qSLIIDRGMALHKMIRLVTLATGGEGWLNFMGNEFGHPEWIDFPREGNGwsyEYCRRQWS 541 Akkermansia muc...
XP_001321781 462 TIafwlmDKEMYTNMSClqpeSMITARGMALHKIIRLITIGLGGEAYLNFMGNEFGHPEWIDFPREGNGnsfLHCCRRFD 541 Trichomonas vag...
Feature 1                                   
CAB91480     571 LTEDGL----------LRYQYLNNFDR 587 Neurospora crassa
AAQ66793     536 LADSPF----------LRYAGLHLFDQ 552 Porphyromonas gingivalis W83
YP_002603621 545 LVDNED----------LMFSCLARFDR 561 Desulfobacterium autotrophicum HRM2
ZP_07357159  560 LKYVGLaawdkaqlwdLAAAHMEDFQQ 586 Desulfovibrio sp. 3_1_syn3
ZP_06241263  541 LADEPG----------LRYHFLRDFDR 557 Victivallis vadensis ATCC BAA-548
ABJ71766     530 LADNEN----------LRFKYLLAFDQ 546 Lactococcus lactis subsp. cremoris SK11
ZP_02870900  538 LVDNGF----------LKYEWLYNFDR 554 candidate division TM7 single-cell isolate TM7a
CAJ69413     540 LSEDNN----------LKYKQLLNFDK 556 Clostridium difficile 630
YP_001878350 542 LVDNPS----------LRFKFLNAFDQ 558 Akkermansia muciniphila ATCC BAA-835
XP_001321781 542 LPFTDH----------LRYKYLLAFDN 558 Trichomonas vaginalis G3

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