1G12,2X3A


Conserved Protein Domain Family
M35_peptidyl-Lys
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cd11306: M35_peptidyl-Lys 
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Peptidase M35 domain of peptidyl-Lys metalloendopeptidases
This family M35 Zn2+-metallopeptidase extracellular domain is mostly found in proteins characterized as peptidyl-Lys metalloendopeptidases (MEP; peptidyllysine metalloproteinase; EC 3.4.24.20), including some well-characterized domains in Aeromonas salmonicida subsp. Achromogenes (AsaP1) and Grifola frondosa (GfMEP). These proteins specifically cleave peptidyl-lysine bonds (-X-Lys- where X may even be Pro) in proteins and peptides. AsaP1 peptidase has been shown to be important in the virulence of A. salmonicida subsp. achromogenes, having a major role in the fish innate immune response. Members of this family contain a unique zinc-binding motif (the aspzincin motif), defined by the HExxH + D motif where an aspartic acid is the third zinc ligand and is found in a GTXDXXYG or similar motif C-terminal to the His zinc ligands.
Links
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Statistics
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PSSM-Id: 199915
Aligned: 10 rows
Threshold Bit Score: 220.399
Created: 30-Sep-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active siteZn binding site
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The conserved Tyr residue, three residues C-terminal to the zinc-binding Asp, acts as a proton donor during catalysis
  • Structure:1G12: Grifola frondosa M35 Zn peptidase binds zinc
    - View structure with Cn3D
  • Comment:11679721
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
1G12_A         4 GCSsSEQSALAAAASAAQSYVAESLSYLQtht-aaTPRYTTWFGSYisSRHSTVLQHYTDMNSNDf---sSYSFDCtCTA 79  Grifola frondosa
2X3A_A       180 RCTnTQKSDLLTALDAASGISNNASSYLAvdk-pdGQRYRSWFGAYssARWDQAETNFSKIKDAIdn--kPLTFDCsCKQ 256 Aeromonas salmo...
NP_643072    198 NCStTRTSQAGSAVTAARNYSQNARNYLNag--stGARYTTWFGTYnaSRYSRVSSNFVNIDNALdqnngQLTINCsCEA 275 Xanthomonas axo...
NP_903176    200 NCSvTQQNQSKDGVTAASAMADETNAYLNgtp-sgTQRYTTWFGKYsqANWTTAKSHYVKIKDALdt--kPIKLDCsCTD 276 Chromobacterium...
ABE55895     204 RCSnSEQSSILSGLSAARSMAANAKSYMQfs--spTTRYNTWFGSYssSRWNTVANNFSNIDSALnn--ePLTFDCkCSK 279 Shewanella deni...
YP_003955867 201 QCSsSQQSNIAQAVNSAKTLAGKAVTYLSdtqpsnTPRYKTWFGSYtsTGWSTMKAHFSTLKAAFnt--kPVVIDCsCTS 278 Stigmatella aur...
P81055         5 GCSaTRQTQLNAAASQAQTYAANALSYLNsht-ssTTRYTTWFGTFvtSRYNTVLSHFSSISSNTf---sSYTFDCtCSD 80  oyster mushroom
Q9Y7F7       188 GCTsSRQTTLVSAAAAAQTYAQSSYNYLSsht-asTTRYVTWFGPYtsARHSTVLSCFSNMLAYPy---aNYEYDCtCTE 263 Armillaria mellea
AEK63262     205 SCSnSQKTALTTARADATSIATNAKSYLNan--ntGSRYTWWFGAVtsGRYTTVTSHYTNISSVLgn--qAYKFDCsCTE 280 Collimonas fung...
EFJ00563     198 SCTsTYRSQTNAAITASSGLASNSISHLEanp-sgSTLQTTWYGTFasSRYRATLSAFEALVDSPs----SWTYDCsCTE 272 Schizophyllum c...

Feature 1                                               ##  #        #  #                        
1G12_A        80 Ag--tFAYVYPnrfGTVYLCgAFWKApttGTDSQAGTLVHESSHFtrngGTKDYAYGQAAAKSLAtmDPDKAVMNADNHE 157 Grifola frondosa
2X3A_A       257 Sy---FAYVYPdqpYKVYLCkSFWTApvtGSDSRAGTIVHQLSHFnvvaGTDDLGYGQANARNLAktDPVKALNNADNHE 333 Aeromonas salmo...
NP_643072    276 DladaYAYVYPnqpYEIHVCnAFWSAsttGTDSKAGTLVHETSHFtvvaGTQDRVYGQSGARSLAisNPAQAITNADSHE 355 Xanthomonas axo...
NP_903176    277 Gg--tYAYVYPsqpYTIYLCgAFWTAptkGTDSKGGTLVHELSHFtvvaGTDDHVYGQAGAKNLAktNPTQALNNADNHE 354 Chromobacterium...
ABE55895     280 Sy---FAYVYPtqpYKVYLCnAFWSAptsGTDSKGGTIIHELSHFnivaGTDDIVYGQSGAKSLAisNPAQAIQNADSHE 356 Shewanella deni...
YP_003955867 279 Na---YAYVYAnrpYTIYVCnAFWGApltGTDSKAGTLIHEMSHFtvvaGTDDHAYGQSAAKSLAmsQPTQARDNADNHE 355 Stigmatella aur...
P81055        81 Sg--tYAFVNPsnfGYVTLCgAFWNApvaGTDSRGGTLIHESSHFtrngGTDDHVYGQAGAQSLArsNPAQAIDNADSHE 158 oyster mushroom
Q9Y7F7       264 Sd--vYAYVYPsqfGTIYLCgAFWQTtttGTDSRGGTLIHESSHFtiicGTQDYAYGQSAAKSLAssNPSEAIKNADNYE 341 Armillaria mellea
AEK63262     281 Sd--tYAYVYPdqpYTVYLCgAYWNApanGTDSKAGTLVHETSHFtvvaGTSDHVYGQTGAHNLAvsNPTNAVDNADNHE 358 Collimonas fung...
EFJ00563     273 Sg--tYAYVYPsqyGEVYLCgYFWQApatGSGSRADTIIHEGTHFpqvlGTDDVTYGESSCKSLArsNPAQAVNNADNHA 350 Schizophyllum c...

Feature 1             
1G12_A       158 YFSEN 162 Grifola frondosa
2X3A_A       334 YFAEN 338 Aeromonas salmonicida subsp. achromogenes
NP_643072    356 YFAEN 360 Xanthomonas axonopodis pv. citri str. 306
NP_903176    355 YFAEN 359 Chromobacterium violaceum ATCC 12472
ABE55895     357 YFAEN 361 Shewanella denitrificans OS217
YP_003955867 356 YFAEN 360 Stigmatella aurantiaca DW4/3-1
P81055       159 YFAEN 163 oyster mushroom
Q9Y7F7       342 YFAEN 346 Armillaria mellea
AEK63262     359 YFAEN 363 Collimonas fungivorans Ter331
EFJ00563     351 FFSDY 355 Schizophyllum commune H4-8

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