C-terminal domain of Formyltransferase and other enzymes
C-terminal domain of formyl transferase and other proteins with diverse enzymatic activities. Proteins found in this family include methionyl-tRNA formyltransferase, ArnA, and 10-formyltetrahydrofolate dehydrogenase. Methionyl-tRNA formyltransferases constitute the majority of the family and also demonstrate greater sequence diversity. Although most proteins with formyltransferase activity contain the C-terminal domain, some formyltransferases ( for example, prokaryotic glycinamide ribonucleotide transformylase (GART)) only have the core catalytic domain, indicating that the C-terminal domain is not a requirement for catalytic activity and may be involved in substrate binding. For example, the C-terminal domain of methionyl-tRNA formyltransferase is involved in the tRNA binding.
Feature 1:substrate binding site [chemical binding site]
Evidence:
Comment:This site describes the binding site for tRNA and formyl-methionine (fMet) at the 3' end of the tRNA.
Structure:2FMT_B; Escherichia coli Methionyl-Trnafmet Formyltransferase dimer complexed with two Formyl-Methionyl-tRNAs; contacts at 4A - View structure with Cn3D
Jiyao W et al.(2023). "The conserved domain database in 2023.",