Conserved Protein Domain Family
mut-7_like_exo

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cd06146: mut-7_like_exo 
DEDDy 3'-5' exonuclease domain of Caenorhabditis elegans mut-7 and similar proteins
The mut-7 subfamily is composed of Caenorhabditis elegans mut-7 and similar proteins found in plants and metazoans. Mut-7 is implicated in posttranscriptional gene silencing. It contains a DEDDy-type DnaQ-like 3'-5' exonuclease domain possessing three conserved sequence motifs, termed ExoI, ExoII and ExoIII, with a specific YX(3)D pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis.
Statistics
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PSSM-Id: 176655
View PSSM: cd06146
Aligned: 18 rows
Threshold Bit Score: 197.901
Threshold Setting Gi: 15223514
Created: 14-Apr-2008
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:catalytic site [active site]
Evidence:
  • Comment:The catalytic site is characterized by the presence of four negatively charged residues (DEDD) that serve as ligands for the two metal ions required for activity.
  • Comment:DEDDy exonucleases contain a conserved tyrosine at the ExoIII motif which is important in catalysis.
  • Citation:PMID 9396823

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                               # #                                       
gi 16549645    96 VHLLASWEDLTRHegal------------lqcHQVVGVDVEWTPVfvag-----------------grprPSLLQVA--- 143
gi 15237941     7 IYLVSSTDSSEFThlkw-----------sftrSTIIALDAEWKPQhsnts----------------sfptVTLLQVAcrl 59
gi 125558189   16 VHLVTGGGSSPEHalllr----------slaaARVVALDAEWKPRrrgpavaddpsaaaaatppppqfptVTLLQVAcrg 85
gi 15223514   356 VEDVVWVDEVNELrkats----------flegCRVVGIDCEWKPNyikgs----------------kqnkVSIMQIG--- 406
gi 116056782  372 VRWVDDVEGLRAAtdavralggsgeastsaraPPCIGLDAEWRPGdn---------------------tpVALLQIA--- 427
gi 168037922   30 ILFVDSPRTEEFEvfli-----------aleeAMIVAMDAEWKPVrrag-----------------vsprVSIMQIScri 81
gi 157110292  437 VHLVDSKDKFYAMlsd-------------lcrQSMIAFDSEWKPTfgg-------------------aneVSLIQLA--- 481
gi 167535101  201 EPIFVGTEEQAAAvladvk--------adaamQRPISFDCEWAGTvsly-----------------ddtrLGIMQLA--- 252
gi 20129683   413 VNKADEFDRMLYHlq----------------qECVIYLDSEWMQSvcg-------------------dnqLCVLQIA--- 454
gi 116056560  358 CVFVDDEKSLLEAshry------------lwnQGMIGIDTEWGADvgedadn-----------adrsgdeVAILQIA--- 411
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                              #          
gi 16549645   144 -------VEGHVFLLDVlalsqpp-tgqgaqaFSRLVAQLLSDp--------------sITKLGYGMVGDLQKLGTSCPa 201
gi 15237941    60 s---hatDVSDVFLIDLssih--------lpsVWELLNDMFVSp--------------dVLKLGFRFKQDLVYLSSTFTq 114
gi 125558189   86 dgdgggaAAAEVFVVDLlavp--------laeLREPLRELFERp--------------eVLKLGFRFKQDLVYLSATFAa 143
gi 15223514   407 -------SDTKIFILDLiklyn-----daseiLDNCLSHILQSkstlklvsltedypdhKLSSGYNFQCDIKQLALSYGd 474
gi 116056782  428 -------TRGEVFLVDLlataprsagealndaTDELLQAVLWSe--------------dVYKLGFSFAYDIKRMKASYSh 486
gi 168037922   82 rkdefitGEEVIFVLDLlals--------aadFAFPLKTMLCSp--------------rILKLGFAFKQDQLHLSASFPg 139
gi 157110292  482 -------TWDDVYMIDVmvsql------epldWAALAKNVFNRd--------------dVLKLSFAPSTDISMFQKALPs 534
gi 167535101  253 -------TKSAVYVIDAcaep-------gvfdFCAFATRLLQL----------------TPIIGFGVMSDRDQLKSAGVd 302
gi 20129683   455 -------TGHNVYLIDClares-----lrsehWRLLGANIFNNv--------------nIRKVGFSMVSDLSVLQRSLPl 508
gi 116056560  412 -------SQLGVVILDVpklly-----scpdtLETTIGKMFEDe--------------eVLKLGFAVQEDLRRLSKCHKa 465
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                         
gi 16549645   202 lahv---ekqiLGGMDLLLVHRQMrvasvpapa--------vdrarelrGLSLLVQQVLGTALDKTQQLSNWDRRPLYEE 270
gi 15237941   115 hgceg-gfqevKQYLDITSIYNYLqhkrfgr-----------kapkdikSLAAICKEMLDISLSKELQCSDWSYRPLTEE 182
gi 125558189  144 algssagfervEPFLDVTNAYYYLkghdmqk-----------rlpretkSLATICEELLGVYLSKELQCSDWSYRPLSEG 212
gi 15223514   475 lkc----feryDMLLDIQNVFNEPf-----------------------gGLAGLTKKILGVSLNKTRRNSDWEQRPLSQN 527
gi 116056782  487 lkvw---sersKNLVDVKQLAFASspskm----------------slrcGLAVLTRQVTGFTLDKKEQCSDWGKRPLTEG 547
gi 168037922  140 peang-cfdkvEPYIDIAKLYKEFlhvnfsnlkhk----gkrfvlggthSLTAISKAVLGHPLCKDAQCSNWEQRPLSQD 214
gi 157110292  535 fnvmy-ssqstSAILDLQLLWRHVerfdsfrfp--------yheesvnqNLANLVRLCLGKKLDKSNQFSNWAQRPLRKE 605
gi 167535101  303 ge------lvgQRVLDVMDTVAQLqqtipvpptyp----dkwqgsspriSLADLVQGLVGVSLAKHEQLADWERRPLRPE 372
gi 20129683   509 qlr----lqmpHHYLDLRNLWLELkkqrfgvelp------fgnvnragdALTDLSLACLGKKLNKSNQCSNWANRPLRRE 578
gi 116056560  466 fk-------tvQGVVDLQKLWKLLvskartrsvsapwsteaellryqpvGLSALVAAVLGKPLDKMMRMSDWSRRPLTQS 538
                         250       260
                  ....*....|....*....|.
Feature 1            #   #             
gi 16549645   271 QVIYAAADAYCLLEVHQALCR 291
gi 15237941   183 QKLYAATDAHCLLQIFDVFEA 203
gi 125558189  213 QIQYAASDAYYLLDIFDLFHQ 233
gi 15223514   528 QLEYAALDAAVLIHIFRHVRD 548
gi 116056782  548 QIAYAAADGHSLCLIFDKCLE 568
gi 168037922  215 QILYAAADTHCLLALFDTLLS 235
gi 157110292  606 QLRYAALDAFCLLEIYDAIEK 626
gi 167535101  373 SLHYAALDAYVLLQVLDILSK 393
gi 20129683   579 QILYAAIDARCLMLIYNTLIE 599
gi 116056560  539 QMEYAALDAWTLVEVHRTLLE 559

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