1WLJ


Conserved Protein Domain Family
REX4_like

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cd06144: REX4_like 
Click on image for an interactive view with Cn3D
DEDDh 3'-5' exonuclease domain of RNA exonuclease 4, XPMC2, Interferon Stimulated Gene product of 20 kDa, and similar proteins
This subfamily is composed of RNA exonuclease 4 (REX4 or Rex4p), XPMC2, Interferon (IFN) Stimulated Gene product of 20 kDa (ISG20), and similar proteins. REX4 is involved in pre-rRNA processing. It controls the ratio between the two forms of 5.8S rRNA in yeast. XPMC2 is a Xenopus gene which was identified through its ability to correct a mitotic defect in fission yeast. The human homolog of XPMC2 (hPMC2) may be involved in angiotensin II-induced adrenal cell cycle progression and cell proliferation. ISG20 is an IFN-induced antiviral exonuclease with a strong preference for single-stranded RNA and minor activity towards single-stranded DNA. These proteins are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. REX proteins function in the processing and maturation of many RNA species, similar to the function of Escherchia coli RNase T.
Statistics
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PSSM-Id: 99847
View PSSM: cd06144
Aligned: 46 rows
Threshold Bit Score: 196.968
Threshold Setting Gi: 145487332
Created: 28-Feb-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #### #                                          #   #                        
1WLJ_A         8 VAMDCEMVGLGph-----reSGLARCSLVNVHGAVLYDKFIRP-EGEITDYRTRVSGVTPQHMVGATPFA--VARLEILQ 79
gi 170083871   9 LAISCANVGVGpgg----ttSMLARVSIVNYRGSTVFDHYVAPtMQVVTDYRSVTTGISESDLYSADAWAfnVVQQYVAN 84
gi 126652498  10 ISIDCEMVGCGeng----hiSVLGRIAVVDDKLKLLMDAFVKP-SMRVTNFRTKWSGLTWDKLKYGESFE--SIQKKFLQ 82
gi 145349060 106 VALDCEFVGVGeag----ekHACARVCVVGSSGEILLHTWVNP-GEEVTDYREELTGATPEKLADAPSLE--RVRAIVVQ 178
gi 881338      9 VAISSVSVGVGprg----etDMLARVAVIDFTGAVLLDVYVAP-TNPVRDYREAKTGIKPEYLYSSRAQDirAVYQTVRQ 83
gi 162681734 121 VALDCEMVGGGsdg----siNICARVCLVDEDENVLLNTYVQP-LLPVTDYRYEITGIKPSDFNGAPSFK--RVRYIVKR 193
gi 145343770   5 LALDCEMVGVGegg----veSMLAQVCVINEYGNTVYLSYSRA-YKTVTDYRTHVSGILPRHVEGSSAREfaDVKRDVAE 79
gi 159478589   1 LALDCEMVGTGpng----svSALARVCLVNGAGAVLLDTFVRP-NEPVTDYRTWVSGVRPEDVARGRPYD--EVVQQVGD 73
gi 163774502 109 YALDCEMVTVRgkgg--amkSALGRISIINATGQVVIDEFVRP-QQPIVSYNTRWSGIRKRDLVDATPFP--KVRDRLRT 183
gi 118349764 432 VAIDCEMVQCEasqkdgfptQELARVSIVNYNGHVLLDTYVRP-QKKIKNYLTKVSGITFTHIKNAPTYP--EVKNKIFE 508
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              ## ###                                                    
1WLJ_A        80 LLKG-------------KLVVGHDLKHDFQALK-EDMSG--YTIYDTSTDRLLWREakld---------------hcrRV 128
gi 170083871  85 LIKG-------------KVLVGHGIWNDLSVLG-IPHPA--VSTRDVALYQPFRNAlrs----------------phqII 132
gi 126652498  83 IVEHyrke-----stsgLVFVGHDISNDFQVLK-WTPPE--SEIRDTCTYFPLRKLliksllekgeithhqkegflrqKP 154
gi 145349060 179 ILLGkavqtrrehvgvkHLLIGHSVEHDLEVLD-IKWKK--GMRRDTAQFPLYLRHth-------------------lPF 236
gi 881338     84 VLRN-------------KVVVGHSMWLDFMVLG-LTHPT--KDTRDVALYLPFRNTlrc-----------------qrMI 130
gi 162681734 194 ILEDg------------PLLIGHDLRHDLACLR-LDHSP--ELIRDTATYQLFVKTsg-------------------vSH 239
gi 145343770  80 LIKG-------------RIVVGHALENDFSALQ-LHHPR--EDTRDTAKWRPLLRPphf-----------------rkPR 126
gi 159478589  74 MVRG-------------RVLVGHAIGHDLKALRlEDHPR--THLRDTSKWPGLMKTlpng---------------rkvSA 123
gi 163774502 184 ILKN-------------ARVVGHAVSNDFRAMN-LTSKElnALVYDTSASSTLKIAalge--------------ndgnAV 235
gi 118349764 509 ILKD-------------KIIVGHSVQHDLSSIK-FEPPKd-KMIRDISNYKELKQSg--------------------kKV 553
                        170       180       190
                 ....*....|....*....|....*....|....*
Feature 1                            #    #         
1WLJ_A       129 SLRVLSERLLHKSIQnslLGHSSVEDARATMELYQ 163
gi 170083871 133 GLQTLAWQLLCRRCQe--GQLHPLENARVALDLYR 165
gi 126652498 155 SLRSLSKHVLNLNIQq--GSHCPWEDAKSTMMLYL 187
gi 145349060 237 KLRALAEQFLGEKIQeegEAHDPCVDARISMRLYQ 271
gi 881338    131 GLWTLNYRLLGLRCSa--APVDPLESARVALNLYR 163
gi 162681734 240 KLRFLTEVFLGYKIQd-gTIHDPCEDAIAAMRLYK 273
gi 145343770 127 RLRHLARDFCALSIQc-gDAHDPAEDALAALAIYR 160
gi 159478589 124 SLKDLAATHLGLAIQq--GEHTPVDDARAALYLYQ 156
gi 163774502 236 SLKRLSKYLLHRTIQe--KQHCSLIDARVTLELYQ 268
gi 118349764 554 SLKKMVKQELGITFQe--GSHDSISDARAALLIYK 586

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