1L3S,1QTM


Conserved Protein Domain Family
DNA_polA_I_Bacillus_like_exo

?
cd06140: DNA_polA_I_Bacillus_like_exo 
Click on image for an interactive view with Cn3D
inactive DEDDy 3'-5' exonuclease domain of Bacillus stearothermophilus DNA polymerase I and similar family-A DNA polymerases
Bacillus stearothermophilus-like Polymerase I (Pol I), a subgroup of the family-A DNA polymerases, contains an inactive DnaQ-like 3'-5' exonuclease domain in the same polypeptide chain as the polymerase region. The exonuclease-like domain of these proteins possess the same fold as the Klenow fragment (KF) of Escherichia coli Pol I, but does not contain the four critical metal-binding residues necessary for activity. The function of this domain is unknown. It might act as a spacer between the polymerase and the 5'-3' exonuclease domains. Some members of this subgroup, such as those from Bacillus sphaericus and Thermus aquaticus, are thermostable DNA polymerases.
Statistics
?
PSSM-Id: 176652
View PSSM: cd06140
Aligned: 97 rows
Threshold Bit Score: 70.7563
Threshold Setting Gi: 5822373
Created: 19-Mar-2008
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
1L3S_A        15 MLADKAALVVEVVeenyhdAPIVGIAVVNe-hGRFFLRPetal-------------adpqFVAWLGDetkkKSMFDSKRA 80
gi 42561209  321 YEDSINYLYVESLeedyhkDKIIGIGISNn-kGNFYLDFknkaqqlsffedttlsstdslFEEFLNNsnlkKYTYDIKKT 399
gi 115252786 309 FNENNTTVYLELLdenyhtSEIIGFGIVNs-kGVFYFDYihak-------------hdklWHQFLNDnkyqKLTYHAKSL 374
gi 15487835    3 AIKTPVAFGVEMLtdnyhtADLIGFYIGAp--DHWYVSDdvall------------aepvLKDWLEDashpKAVFGVKRT 68
gi 160914226 305 LLVDGSVLYANLDaenyydATLYGFAIAHg-nNCEYISLedakn------------dtalLEYIHTDn--gKIVYDAKNV 369
gi 170017479 326 MMVDKVAVHIEMSgsnyhtADMIGFVIGNp-aTGYYASRdmsll------------qkpeLRAVIENgdikKNVFNVKAQ 392
gi 116495190 317 KLTEPLTFEIEMLednyhvAEQIGFFIGTk--EETYVSTdvtll------------tlpaVKRWLEDakrdLTVFDGKRN 382
gi 118586552 345 EIKNYLTVYFQTDsdnyhtAKISAFVVGNe-nDGYFASRdlell-----------nssvvRKLFKGDl--pKYVFNAKEL 410
gi 50365399  318 FSCETNFLYIESIdenyhkGQILGLAICNe--KGFFYLNdiin--------------dleLEQFFLNdnfkKNTYDIKKT 381
gi 6014999   311 SISQIRSKEIPLMf----vQGEKCFYLYDqesNTVFITSnk------------------lLIEEILKsdtvKIMYDLKNI 368
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
1L3S_A        81 AVALKWKGIELc--GVSFDLLLAAYLLDpa-----qgVDDVAAAAKMKQYeavrpdeavygkg--akravpdepvlaeHL 151
gi 42561209  400 TYLLKNHKYNVlasNFDFDFMVACYSLNan------vISDLSNQIKLVDNmieletideifgkgvkknpdidldikskYI 473
gi 115252786 375 IVALARDNIFVq--NIEYDMMLAGYVYNsna---knsLDTYINLFEKKQIltdeffygkgvk----kqipadviklghFI 445
gi 15487835   69 QVAANRLGVHLa--GVDFDLLLASYLLNttnn--sndLGTVANLHEYQNVqtdeavygkgak----ravptepallyqHL 140
gi 160914226 370 YHAWHRNHIAFv--PVHRDVMLEAFLLDgmi----sdYDKLAEKYAFDCTlskedvfgkkgk-----pklvveedaaaYA 438
gi 170017479 393 LILFKRLGLTLk--GAQFDFLLVAYLLDttdn--dneMSTLAQRFDVYLTedeeiygkgaky-----avpddeelvlmHL 463
gi 116495190 383 IVAANRLGVKLp--DIAFDVLLASYLINpden--sndLGKIAEDHDYHDLprdediygkgak-----rqvpeddklfgQF 453
gi 118586552 411 IVLLNRLGLQIn--NLDFDFLLVSYLLDttdn--dnqLATLANRFNVFLEsdaevygsgvkf-----kipennetlfsHM 481
gi 50365399  382 VTLLRNNNIKFsfsSFTYDAMIASYVLNpnvtsriqnLANIVETELFVEEdelfygkgvkq------nkeidtaskakFI 455
gi 6014999   369 FHQLNLEDTNNi--KNCEDVMIASYVLDstrs--syeLETLFVSYLNTDIeav----------------------kkdKK 422
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|
1L3S_A       152 VRKAAAIWELERPFLDELrrneQDRLLVELEQPLSSILAE 191
gi 42561209  474 SKKAYLLKKYSDQLIEQLkqtnTYDLYLKIDHPLIEVLYD 513
gi 115252786 446 GEKASYIHKLRPKIIELLktnqQYDLYHNIELPIAFALAR 485
gi 15487835  141 VQKAAAIYHLQPQLEEQLkehqQFDLFTDMELPLALVLAQ 180
gi 160914226 439 MQCAENLANIEQQLFDELeqkdMKELYDTIEMPVVEVLYA 478
gi 170017479 464 AHKADALIQLEMPALSALqshqQTHLYTEIELPLAHVLAE 503
gi 116495190 454 ARKSDALFALRPDLTGDLekqeQTDLFTDMEMPLSRVLAE 493
gi 118586552 482 ANKASTISKLHDPSMKKLsdhqQAELYRQIELPLSKVLAS 521
gi 50365399  456 VGKLELLYSTHKPIINLLkennQFNLYEKIELPLVEVLFN 495
gi 6014999   423 IVSVVLLKRLWDELLRLIdlnsCQFLYESIERPLIPVLYE 462
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap