Conserved Protein Domain Family
H2MP_like-2
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cd06070: H2MP_like-2 
Putative [NiFe] hydrogenase-specific C-terminal protease. Sequence comparison shows similarity to hydrogenase specific C-terminal endopeptidases, also called Hydrogen Maturation Proteases (H2MP). Maturation of [FeNi] hydrogenases includes formation of the nickel metallocenter, proteolytic processing and assembly with other subunits. Hydrogenase maturation endopeptidases are responsible for the proteolytic processing, liberating a short C-terminal peptide by cleaving after a His or an Arg residue, e.g., HycI (E. coli) is involved in processing of HypE (the large subunit of hydrogenases 3). This cleavage is nickel dependent.
Links
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Statistics
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PSSM-Id: 99879
Aligned: 6 rows
Threshold Bit Score: 154.125
Created: 10-Jan-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                    #                                           #                       
YP_929721      3 LVVGLGNVVYGDDGFGSCLAQVLSh---yNDFVFDGNAhGIGVLGTLadyDILVFLDIDvr-----lpPGAVAIERv--- 71  Pyrobaculum isl...
YP_001435094   5 LVLGLGNRNLGDDAVGSCLAEALAarapeGVEVKDGDDmGLGLLGFLegyDLVIFVDASpdv----edVEAFLIEAe--- 77  Ignicoccus hosp...
YP_919997      8 LIMGVGNRMYGDDAFGPLFAEALEq---cGVPALNSELdVFTAVSHLegvDLLIFIDVVdksfgepgdVVVLRIDPrtls 84  Thermofilum pen...
YP_001191012   4 LIAGVGNIFMGDDGCGSIVAEALKgc-vsGAEVIDIGTgGLPLTDYLenfDVVILVDAAvid----edVKVIEVTDem-n 77  Metallosphaera ...
CAC86895       4 KIIGLGNRLYGDDAVGSLTAACMEe---lGLPAFDAGAnGFQALSAIengDIVFFIDIVqmd----eeEGIFKVDLd--- 73  Acidianus ambiv...
NP_342670      3 AIIGVGNRLMKDDGFGSCLAEVIInk-vkNAVVVDLGL-GNLLSVDLdkyDTVIIIDVAnin----eeYGIFKITSt--- 73  Sulfolobus solf...

Feature 1                        #                                                        
YP_929721     72 eGSLTLQetr--liDAHRTPPSLLVGYLramg-rdpKAYIIAVGPKTLepfsPPSQEVIKAVPIAVEELKKLL 141 Pyrobaculum islandicum...
YP_001435094  78 kAELEEArem--vqDSHKAGPVALAVLAkksglfqgKAYFVAFKPESLcfmcKPSEASLERALKAAELVNKLL 148 Ignicoccus hospitalis ...
YP_919997     85 pEEVATAisf--etSAHQVTPAHIVLLGyssgffrgEAYVVGVVSEHTdfarKMSSRVVAAAERACLKLREIA 155 Thermofilum pendens Hrk 5
YP_001191012  78 pDSVGEGvlsllfgGSHGLGVMDLLNFLkire-rkpKVLIVGCRPISVevrmGLSEGMTANCLKALDEIARLG 149 Metallosphaera sedula ...
CAC86895      74 kADFVEIt------DPHRLTPLQVLSLSarsnnrpkEAYIVGIKPEYIdw-pGISNAAIKRLEKVLQKFKKFI 139 Acidianus ambivalens
NP_342670     74 sKGILEQ-------SLHELGLNTILKLYed-----kQFYIIVCKPEEIqigyGLSKDCLLRIEKLIPEFKAFL 134 Sulfolobus solfataricu...

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