Conserved Protein Domain Family
ACS

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cd05966: ACS 
Click on image for an interactive view with Cn3D
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme)
Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Statistics
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PSSM-Id: 213313
View PSSM: cd05966
Aligned: 49 rows
Threshold Bit Score: 970.863
Threshold Setting Gi: 113867627
Created: 10-Dec-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 35 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1PG3_A        28 YKQSINDPDTFWGEqgkiLDWITPYQKVkntsfapgnvSIKWYEDGTLNLAANCLDRHLQE-NGDRTAIIWEGDDTsqs- 105
gi 83815833   36 YDRFDDFPEGFEEYad-lLDWDTRWDEVfdgs---dppFFEWFTGGELNACYNCVDRHLHE-RPNQAAFIWEGEDGtr-- 108
gi 113867627  52 ERFSLERFPECFKEfadlLDWYKYWETTldts---nppFWRWFVGGRINACHNCVDRHLAA-YRNKTAIHFVPEPEdea- 126
gi 11497978   41 DKESIEKWWEKWANe---IFWFRKWDRAldds---nppFYRWFVGGETNLAYLCTDWQIEQgRKNKVAIIWEGEPVkeng 114
gi 15899575   32 HSQTVKDYKQFWASvaseIDWFKPWEKTldds---nppFYKWFVGGELNASYLTVDRHVKSwRKNKVAIIWEGEPVdekg 108
gi 16081543   25 YRKSLEDPENFWAKqaeiLTWYSKWNKIldds---kkpFYRWFVGGKLNVSYNAVDRHLEQhRRNKAAYIWVGENGee-- 99
gi 161528209  17 RIKADSDFVSFWNDqaknLTWFSPWNETldw----qppFAKWFVGGTINASHNALDVHQDS-KSEKPAILWEGENGds-- 89
gi 45357711   17 QQKALDDPEKFWGEqakcLEWDKTWDKAldw----nppYAKWFKGGSLNASYNCVDRHIKGnRRNKAAIICEKEDGsc-- 90
gi 57234024   29 YNSALTDSEGFWAKhsdvLSWEKPWEKVldw----nppYARWFVGGKLNMSYQCVDRHAKSwRKSKVAIYWEGENGdt-- 102
gi 117928275  55 SEQNFPECFRVYADl---LSWDAYWHTTldts---nppFWKWFVGGRLNACYNCVDRHLAA-SANKAAFIWVPEPEnep- 126
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                       ###              
1PG3_A       106 -----khISYRELHRDVCRFANTLLDLGi-kKGDVVAIYmPMVPEAAVAMLACARIGAVHSVIFGGFSpEAVAGCIIDss 179
gi 83815833  109 -----rtLTYRDLYREVNKMAASLRDVGv-qEDDVVTLHlPMVPALPITMLACARIGAPHSVVFGGFSaSALAQRATDad 182
gi 113867627 127 ----vhhLTYQELFVRVNELAALLREFCglkAGDRVTLHmPMVAELPITMLACARIGVIHSQVFSGFSgKACAERIADse 202
gi 11497978  115 epvevrkLTYYDLYRESNRIAAMLKDKLgvkKDEILTFYlPMIPELPLFMLGVQRIGAKHSIVFSGFSaQALAVRIMDag 194
gi 15899575  109 npkevrkLTYYDLYREVNRVAYLLKEKYglkKGDAIAIYlPMIPELPIFMLAAARIGVVFTVVFSGFSaDALASRINDae 188
gi 16081543  100 -----kiVTYDGLYRRINNLAKALLNLGi-kKGDRIVLYlPMIIEAPVAMLAAARIGAVFSFVFAGFGaGALAERINDsk 173
gi 161528209  90 -----riLTYGQILTEVQKFSNILKSLGv-eKGDRVTLYlPMIPELPIAMLACARIGATHTVIFSGFSaTSIRDRVDDsk 163
gi 45357711   91 -----giLTYYELYREVNKFANVLENLGv-eKGDVVTIYmPMMTEAIIAMLACSRIGAIHNVVFSGFSsDALADRINDsk 164
gi 57234024  103 -----qtISYSDLYENVNRYASVLKKLGi-gKGDRVTVYlPMIPEMVYILLACNRVGAIHNVIFSGFSsQSIADRVNDsg 176
gi 117928275 127 ----tlaLTYQELYRRVNEFAALLRDFAgvkTGDRVTLHlPMVPELPIAMLACARLGAIHSQVFAGFSgAACGDRIADae 202
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #  # #                                                               
1PG3_A       180 SRLVITADEGVRAGRSIPLKkNVDDALknpn--vtsvEHVIVLKrtgsd----idwqeGRDLWWRDLIekas--pehqPE 251
gi 83815833  183 SDVIVTIDGYYRRGEFLHHKeKADTAVeead---tdiDTVLVWErhegtlhpeadleeGRDILVSELLanner-arvePV 258
gi 113867627 203 SRLLITMDAYHRGGELLDHKeKADIAVaeaasagqqvEKVLIWQrypgkyssaallvkGRDVILNDVLagfrg-rrvePE 281
gi 11497978  195 ARYVVTADGVYRRGKVLNLKaIVDDAVkiceqeghkvEKVIVVKragne----ipwndSRDVWFEDLMadigknvkveCE 270
gi 15899575  189 AKLLITADGGWRRGKVVDLKgIVDKALekt----ptiKDVIVVRrignk----vnmveGRDKYFDEVIkdipqnvyvePE 260
gi 16081543  174 AVLLITADGGFRNGKVVELKkIADEALemt----stvRNVIVIKharnd----vnmveDRDIWYHEIVgdsh--tyvePE 243
gi 161528209 164 SKVIVTADGGYRRGKIVKLKeVVDEAIedf----dfvKNVVVVErtkne-----ipmtCKDKLWNDLMndas--dncpAE 232
gi 45357711  165 SKILVTTNLLYRRGKEIDLKkILDKALlnc----nsiKHVVYAKrgiee----fnlvsGKEYFWDELMggak--syvePV 234
gi 57234024  177 SKMVVTASGGHRRGKILPLKeIVDEAVkst----psvEHVLVIKytgye----vvmdpARDVWAHDMLkdad--kyvaPE 246
gi 117928275 203 SKVLITMDGYYRAGDLLDHKvKADEAVaraaqqgctvEKVLVFRrhagqyaskspmvdGRDFFVDDILpqfrn-klvaPV 281
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                             #    ##                    
1PG3_A       252 AMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLAcGATTLMFE 331
gi 83815833  259 SRDAEDTLFLMYTSGTTGKPKGAQHRTGGYLSYVAGTSKYVLDIKPNDTYWCAADIGWITGHSYIVYGPLAlGTTSVMRE 338
gi 113867627 282 PMPAEAPLFLMYTSGTTGRPKGCQHSTGGYLSYVAWTSKYIQDIHPEDVYWCMADIGWITGHSYIVYGPLAlAASSVVYE 361
gi 11497978  271 KMGSEDFSFILYTSGTTGRPKGAQHSVGGYAVHLYTTMKLIFDIKDDDIFWCTADIGWITGHSYIVYGPLMcGATTMMYE 350
gi 15899575  261 RMKSEDPLYILYTSGTTGKPKGIVHDIGGYMTLLHATMKWVFDIRDDDIYWCTADIGWVTGHSYIVFGPLMeGATEIMYE 340
gi 16081543  244 HMDANDPLFILYTSGTTGKPKGAVHSTGGYSVWVASTLKWAFNPDEDDRWWCAADIGWITGHSYIVFAPLIlGLTSIMYE 323
gi 161528209 233 KLDSAHPLYILYTSGTTGKPKGVLHGTGGYLTHLYSTFKWAFDIKDSDVFFCTADIGWVTGHSYVVYAPLLhGATEIMYE 312
gi 45357711  235 PVESEHPLFILYTSGTTGSPKGVVHSTGGYLTYATKTMDWTWGLNEMDVFWCTADIGWITGHTYVVYGPLSlGATIVLYE 314
gi 57234024  247 AMDSTDPLFILYTSGTTGKPKGILHGTGGYGVWACNTLKWAFKPTDESVFWCTADVGWITGHTYVVYAPLAlGLTQVIYE 326
gi 117928275 282 SMPAESTLFLMYTSGTTGRPKGCQHSTGGYLSYVAGTSKYYLDIHPEDTYWCFADIGWITGHSYIVYGPLAlGTTSVMYE 361
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ###                       ##  #                      ####                     #
1PG3_A       332 GVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKaieGTDRSSLRILGSVGEPINPEAWEWYWKkIGKEKCPVVD 411
gi 83815833  339 GAPDHPHKGVTWEIAERHDVDIFHTSPTAVRMYMKWGKEh-pASYDFNFRHMTTVGEPIQPEAWLWYYThIGNEDAVIVD 417
gi 113867627 362 GVPTWPDAGRPWRIAESLGVNIFHTSPTAIRALRRNGPDe-pAKYDCHFKHMTTVGEPIEPEVWKWYHReVGKGEAVIVD 440
gi 11497978  351 GAPDFPDVSRWAAMIERYGVTIFYTAPTAIRMLMRFPEEnytKYDMSTLRIIHSVGEPINPEAWRWYYRvFGREDVVCSS 430
gi 15899575  341 GALDYPQPDRWVSIIERYGITILYTSPTAIRSFMKYGDNwvkAHITSTVRLMHSVGEPINPEAWEWLWKlVGREEVPFGS 420
gi 16081543  324 GSITYPEPDRLWEIIEKYRVNILYTSPTAIRTLMKFGEKypqRHDLSTLKVLGTVGEPINPAAWKWYYEiIGNSRCPIID 403
gi 161528209 313 GAPDFPDASRMWDILQKYKATIFYTTPTALRMFMKFGDDipnSFDLSTLRLLGTVGEPINPEVWRWYFKtIGKEKCPIID 392
gi 45357711  315 GAIDYPEPDRLWGIVENHGVTILYTAPTAIRMLMMYGEKwvnTHDLSTLRLLGSVGEPINPRAWKWYYKvIGKEKCPICD 394
gi 57234024  327 GAPDYPSVDRWWEIIDKYGVSIFYTSPTAIRMFMRHGEElpaKHNLGTLEMLGSVGEPINPEAWEWYYKnIGHENCPISD 406
gi 117928275 362 GTPTYPDAGRPWRIAERLGVNIFHTSPTTIRMLRKVGPDe-pAKYNYHFKLMTTVGEPIEPDVWRWYYDvIGKGEAVITD 440
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #####                                                                           
1PG3_A       412 TWWQTETGGfMITPLPGa---iELKAGSATRPFFGVQPALVDNeghpq--egatEGNLVITDSWPGQARTLFGDHERFEq 486
gi 83815833  418 TWWQTETGGhLITNLPAl---qDMKPGSAGRPCPGIQPAIYDNngtpveaasgqAGNLVIERPWPGMLQTVYGDDQRFIn 494
gi 113867627 441 TWWQTENGGfLCSTLPGi---hPMKPGSTGPGIPGIHPVIFDEegnevpagsgkAGNICIRNPWPGIFQTVWKDPDRYVr 517
gi 11497978  431 TWWMTETGGmLTDHFPGlgkiiPLKPGTNGYPIPGVRMEVLDDegnsv--kpgeRGYLVISTPWPGMLMTLFKDPNRFId 508
gi 15899575  421 TWWMTETGGiLISHLPGly-lvPMKPGTNGPPILGIEPDVVNEdgkqv--nleeRGYLVIKNPWPGMPLTIYKDPERYVk 497
gi 16081543  404 TYWQTETGGfMIAPQRGlg-lpPLKPGSATFPLPGVDPAILDDagkev--rtgeKGYIVFRRPWPGMLMTVNNDDERYVk 480
gi 161528209 393 TWWQTETGGmLISPLPGle-tiPLKPGSGTLPIPGVNITVVDEngkdv--epntKGYLVVKNPWPGMLLTLWGDDEKYKt 469
gi 45357711  395 CYWQTETGGhIIYPPIGiq-svPLKPGSATFPGIGIDVDVVDNegnpv--danvKGNLIIKRPWPGMLAGLWNNDERYRa 471
gi 57234024  407 TWWQTETGGfMITPCPGiq-sfPLKPGSATLPLPGVDPVVVDAegkel--paneIGFIAIRKPWPGIMLGIYNGDELYKk 483
gi 117928275 441 TWWQTETGGfLGCQLPAl---aPMKPGSCGPGVLGVFPVIYDEngevvppgsgrAGNMCIRNPWPGVFQGIWKQPERFVq 517
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #           #  #       ####                              
1PG3_A       487 TYFSTFk----------NMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHAIKG 556
gi 83815833  495 EYWRRFsdvds-ddwrdWVYEAGDGAVHAQDGYFRVLGRLDDVMNVAGHRLGTMELESAVAQVSEVAEAAVAARQDDQKG 573
gi 113867627 518 QYYARYcknpdskdwhdWPYMAGDGAMQAADGYFRILGRIDDVINVSGHRLGTKEIESAALLVPDVAEAAVVPVADEVKG 597
gi 11497978  509 VYFGKYks-------kgWYYYTGDFAMFDEDGYVWVLGRADDILKVAGHRIGTAEIESAMVSHPDVAEAACVGKSDPVKG 581
gi 15899575  498 VYWSRFp----------GMFYVGDYAVKDKDGYFWILGRADEVIKVAGHRLGTYELESALIEHPAVAEAAVIGVPDPVKG 567
gi 16081543  481 TYFSKFp----------GYYYCGDYAVKDEDGYYWLLGRSDEVMNVSGHRLGTIEIEDAIVATGLAVEAAVFGKPDPVKG 550
gi 161528209 470 VYWSKYe----------NCYYPGDYALKDADGYLWLLGRADDVLKVAGHRIGTAELESCIVSHDDVAESAACGIPDEVKG 539
gi 45357711  472 AYWDRFk----------NKFSTSDYAIKDQDGYIWVLGRSDEVLNVSGHRIGTAELEHELVSNKMVAESAVVGKPDDVKG 541
gi 57234024  484 TYWSRFp----------GWYCPGDFSMKDSDGYLWLLGRADEVIKVAGHRISTAELEHALVGHSSVAEAAVASRPDEVKG 553
gi 117928275 518 TYYAKYcrnpeskdwrdWPYFSGDGAVQAADGYFRILGRVDDVINVAGHRLGTKELESATLTVPEIAEAAAVPVMDELRG 597
                        570       580       590       600       610       620       630       640
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                     #    ##                                            
1PG3_A       557 QAIYAYVTLNHGeep--spELYAEVRNWVRKEIGPLATPDVLHWTDs-LPKTRSGKIMRRILRKIAagdtsNLGDTSTLA 633
gi 83815833  574 NVPDVYVTPRDGvaa--sdELRQDIVAAVEKEIGAFARPGNVIFVGd-LPKTRSGKIMRRLLENISng--kDLGDTTTLR 648
gi 113867627 598 KVPDLYVSLKPGlsp--siKIANKVSAAVVSQIGAIARPHRVVIVPd-MPKTRSGKIMRRVLAAISnh--qEPGDVSTLA 672
gi 11497978  582 EIPIIYVVLREGvep--spDMVNELKKHLRATLGPVVASDAVITFVntLPKTRSGKIMRRLLRAVAeg--kELGDVTTLE 657
gi 15899575  568 EVPYAFVILRQGytp--ntQLSQEILKTVRDKVGPIATIDKIFFVGk-LPKTRSGKIMRRVVRAVAtr--aEVGDITTLE 642
gi 16081543  551 EAISAFVVPKDAnad--rhELISEIRKRIRADLGPIYVPDEIRIVKl-LPKTRSGKIMRRVVKAVAln--qIPGDLTTLE 625
gi 161528209 540 EVIIAFVVLKEGint-etkVLEKELVEKIRTDIGAIATPKQIYFVSk-LPKTRSGKIMRRLLKAIGnn--eKIGDVSTLE 615
gi 45357711  542 EVPVAFVILNEKyrelpsnEVKAELIKHIRDTVGPIGTPAMIFFVNk-LPKTRSGKIMRRILKKLImg--eEIGDVTTLE 618
gi 57234024  554 EAIVVFVTLKKDvea--saDVKRELTHHLRTAIGTIATPEEIIFVEk-LPKTRSGKIMRRLLKAVAne--vPIGDTTTLD 628
gi 117928275 598 RAVEMYVTLKPGvaa--stEITEKVTKAIETEIGKIARPKSIWIVSd-LPKTRSAKIMRRILAGISnf--tDVGDTTTLA 672

                 .
Feature 1         
1PG3_A       634 D 634
gi 83815833  649 D 649
gi 113867627 673 N 673
gi 11497978  658 S 658
gi 15899575  643 D 643
gi 16081543  626 D 626
gi 161528209 616 D 616
gi 45357711  619 D 619
gi 57234024  629 D 629
gi 117928275 673 N 673

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