3RAM


Conserved Protein Domain Family
M20_ACY1L2-like

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cd05672: M20_ACY1L2-like 
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M20 Peptidase aminoacylase 1-like protein 2-like, amidohydrolase subfamily
Peptidase M20 family, aminoacylase 1-like protein 2 (ACY1L2; amidohydrolase)-like subfamily. This group contains many uncharacterized proteins predicted as amidohydrolases, including gene products of abgA and abgB that catalyze the cleavage of p-aminobenzoyl-glutamate, a folate catabolite in Escherichia coli, to p-aminobenzoate and glutamate. p-Aminobenzoyl-glutamate utilization is catalyzed by the abg region gene product, AbgT. This subfamily includes Staphylococcus aureus antibiotic resistance factor HmrA that has been shown to participate in methicillin resistance mechanisms in vivo in the presence of beta-lactams. Aminoacylase 1 (ACY1) proteins are a class of zinc binding homodimeric enzymes involved in hydrolysis of N-acetylated proteins. N-terminal acetylation of proteins is a widespread and highly conserved process that is involved in the protection and stability of proteins. Several types of aminoacylases can be distinguished on the basis of substrate specificity. ACY1 breaks down cytosolic aliphatic N-acyl-alpha-amino acids (except L-aspartate), especially N-acetyl-methionine and acetyl-glutamate into L-amino acids and an acyl group. However, ACY1 can also catalyze the reverse reaction, the synthesis of acetylated amino acids. ACY1 may also play a role in xenobiotic bioactivation as well as the inter-organ processing of amino acid-conjugated xenobiotic derivatives (S-substituted-N-acetyl-L-cysteine).
Statistics
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PSSM-Id: 349921
Aligned: 43 rows
Threshold Bit Score: 405.408
Created: 6-Apr-2007
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
metal binding
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1: metal binding site [ion binding site], 5 residue positions
Conserved feature residue pattern:C H E H [KH]Click to see conserved feature residue pattern help
Evidence:
  • Structure:3RAM: Staphylococcus aureus endopeptidase antibiotic resistance factor HmrA binds two zinc ions
    View structure with Cn3D
  • Comment:conserved metal-coordinating cysteine in this subfamily differs from archetypal M20 motif

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
3RAM_A     11 IETNKYSYIEISHRIHERPELGNEEiFASRTLIDRLKehDFEIETEIAghaTGFIATYDsgLDGP--AIGFLAEYDALPg 88  Staphylococcus aur...
NP_832917  17 IERNKEKYIETSHDIHANPEIGNQEfYASRTLSLLLGsaGFQLQHNIAgheTGFIARKSsgKQGP--AIAFLAEYDALPg 94  Bacillus cereus AT...
CAG75864   14 IEQHREEYIAISQDIHAHPETGNNEyYASGLLTDLLKkhLFTVTLNVAgheTAFYAEKDsgKPGP--TIAYLAEYDALId 91  Erwinia carotovora...
CAJ67613   16 SEEKKTSFEKVSDYLFSNPELAFEEyKSQKALCDLLEenEFNVTKGVGgleTSFEAVYSngTNGK--TVAFLAEYDALPg 93  Clostridium diffic...
CAJ69306    9 ADSKKEKILGLCQSLYDEPEIALQEyKSAKKISEFLReeGFDVEENLAgmaTAFKATKKn-GDGP--KIAFIAEYDALPg 85  Clostridium diffic...
CAJ69991   13 ENELGKEIEEVCNFIFNNPELGEEEyISSKYLVEKMKeyGFDTVYPYCnmeTAFRAELGd-NDGP--TIAFLAEYDALPg 89  Clostridium diffic...
AAO36929    9 IDNIALELNNMADNIFDNPEEGLKEyKACDLLCDYLKekEFKVEKGIGgfeTAFRAVYEfgTGGP--SIGLLCEYDALAn 86  Clostridium tetani...
CAC11380    2 RKEIRDETLEVSRRIYEYAELGSQEyRSSRLIADHLSrhGFNVEMPYGgmeTAFRATCY--RGGP--SVGVLAEYDALP- 76  Thermoplasma acido...
EDK87675    9 IDSISSEILAMADSIFDDPELGLNEfRAMKKITDFLEknNFEVEKNIYgfeTAFRAKYTsgKGGI--NIGLLCEYDALEg 86  Fusobacterium nucl...
CBE68518   11 VDRLSSTLIATSRFLHRHPELAYEErESVQHLASLLKahGFEVIEGVGglpTAFMAMAG--VDQPsaTIAFLAEYDALPs 88  NC10 bacterium 'Du...
Feature 1               # #                                   #                               
3RAM_A     89 ------LGHACGHNIIGTASVLGAIGLKQVIDQIgg--kVVVLGCPAEEGGen------gSAKASYVKAGVIDQIDIALX 154 Staphylococcus aur...
NP_832917  95 ------LGHACGHNLIGTISVAAAIALSETLEEIgg--eVVVFGTPAEEGGpn------gSAKSSYVKAGLFKDIDAALM 160 Bacillus cereus AT...
CAG75864   92 ------IGHACGHNIIGVTSLAAAIALAEVIDQTgg--kVVVLGTPAEEGGlrgkggingNVKARFVEHGFLDKVDVALM 163 Erwinia carotovora...
CAJ67613   94 ------MGHACGHNIIGTSSVGAGIILKEIMKKHniegtVKVFGTPAEERV---------GGKITMIKEGVFNNVDAALI 158 Clostridium diffic...
CAJ69306   86 ------NGHACGHHLIASMGVGAGIALSSILDTYkg--eVSIIGTPAEETG---------DGKPYLIEHGVFDGYDAAMM 148 Clostridium diffic...
CAJ69991   90 ygenkePAHACGHNWIAASTLGACIVLSKLKENFkg--kIVLIGTPAEETT---------GGKCDLVKAGAFDDVDVSYQ 158 Clostridium diffic...
AAO36929   87 ------IGHACGHHMQGPAILGAAIALKEVVKDQpf--kVVVYGTPAEETI---------GGKLQMVKEGCFKDIDVAMM 149 Clostridium tetani...
CAC11380   77 ------NGHSCGHNLIAAWAFGTAVTLCEDFGIS-----VTVFGTPSEEGIgp-----yaGSKAILVEKNLIRDVDFVIG 140 Thermoplasma acido...
EDK87675   87 ------LGHACAHHMQGPSIIATAVALKEVLKDYdf--nIIVYGTPAEETL---------GAKVAMDKKGAFRDIDVALM 149 Fusobacterium nucl...
CBE68518   89 ------LGHACGHNLIASSSIGAALALQPFLSTLgg--qILVVGCPAEEKG---------GGKIALVKAGLFANVDAALL 151 NC10 bacterium 'Du...
Feature 1      #                                                                              
3RAM_A    155 IHPgnetYKTIDTLAVDVLDVKFYGKSAHAsENADEALNALDAXISYFNGVaqLRQHIKKDQ--RVHGVILDGGk-AANI 231 Staphylococcus aur...
NP_832917 161 IHPsgktATTSPSLAVDPLDFHFYGKTAHAaASPEEGINALDAVIQLYNSInaLRQQLPSDV--RIHGVITEGGk-APNI 237 Bacillus cereus AT...
CAG75864  164 VHPsgktRLTGPSLANNHLYFHFYGKPSHAgSSPHKGVNALDALVLLYNGIsvLRQQLPDGV--RVHGIITNGGq-APNV 240 Erwinia carotovora...
CAJ67613  159 LHPsdasMPDDISFAQVNLKFDFTGKASHAaAFPWEGKSALSGVIALFNSVnsMRLHLKDYA--RVHGIITDGGs-IHNI 235 Clostridium diffic...
CAJ69306  149 IHPnsktCVTPEIIAIGGLDFIFTGKASHAgAKPYNGINALDAVVLLYNNInaLRQQLVDGT--RIHGIILEAGt-AANV 225 Clostridium diffic...
CAJ69991  159 MHIeafnNINCKALAIDSLEFSFEGVSAHAaSHPHMGVNALDAVQLTFAGInaLRQHVKSDV--RIHGIVSNGGe-APNI 235 Clostridium diffic...
AAO36929  150 MHGgpetTTDVKSLAMSKFKVIFNGKSAHAaLKPENGKSALDALILSFQGIefMREHVVDDV--RMHYTILSAGgtAANV 227 Clostridium tetani...
CAC11380  141 MHPddrwAVGSVSLADLTIEAEFSGKSSHL-LGPDYGINALTPAIETYIALnnLRSSISDLKrpIIGMIIREGGk-ASNV 218 Thermoplasma acido...
EDK87675  150 MHGspmtTTDVKSMALSNFDIIFHGVSSHAaLAPENGRSALDGLLILFQGIefLREHVKEDT--KMHYTIVDAGg-PANV 226 Fusobacterium nucl...
CBE68518  152 VHPsnrtEIFKSALAMRALRVEFFGKASHAaAAPHLGINALDAVVLAFTNLnaLRQQLRDDA--RIHGIITDGGr-APNI 228 NC10 bacterium 'Du...
Feature 1                                                                                     
3RAM_A    232 IPDYTHARFYTRAxtrkeLDILTEKVNQIARGAAIQTGCDYEFGPIqngvNEFIKTPKLDDLFAKYAEEVGeaviDDDFG 311 Staphylococcus aur...
NP_832917 238 IPDYAAARFFIRAatrkrCAEVTEKVRNIAEGAALATGAKVKIHQFqneiDELLVTKTYNDIVAEELELLGe--dVNRKE 315 Bacillus cereus AT...
CAG75864  241 IPEYASAHYYIRAktreeVLALEPRIRAIADGVALATGTTVKIEHQvg-pRDFLINHALNAILLEEFTAAGe--vVDQNA 317 Erwinia carotovora...
CAJ67613  236 IPEKSTAIFNVRAlsieyLNEICEMLKNCAKGAAISTGTNVEIVQLdeiyKEIKNDSELVNIVRQNFEVLGed-yVERDL 314 Clostridium diffic...
CAJ69306  226 IPDMGKVRLEIRAkeqnyFDEVVEKVKNCARGAAIATGCELEFYHFeptcQGLNENKVLVDIFTKIMEEFG----IYEDE 301 Clostridium diffic...
CAJ69991  236 VPEKAACKFFVRAaersyLDEVTKKVINCAKGAELMTGAKLSYRYFensfDNIINNKVLQKITKNNLIEAGitdiLEGKD 315 Clostridium diffic...
AAO36929  228 VPAQAIGSFYVRSynrdyLNDVIRRFRNVMKGAALMTDTEYEIITEke-iDNKIPVLSLNDIVMENAKLVNap-tIRPPR 305 Clostridium tetani...
CAC11380  219 IPDRSVMEIDLRSskrryLEDLAARAKDIIGNISRMHGCDFRIRDVtpvyEEYISNETIDNTLEEELRSHG----IDPVN 294 Thermoplasma acido...
EDK87675  227 VPKYAKAKVSLRSydrkyLDDVVRRFKKVVEGAAMMTETTCEIIETks-lDSKIPVLSLNRILMENAEFVNap-rIQAPR 304 Fusobacterium nucl...
CBE68518  229 IPDYAAARFCIRAlnleyLHDLHRRVVACFEAAAMATGTTVAIHEEgeeyHPMKCNRALGALFYANLETLGer-vEQTPE 307 NC10 bacterium 'Du...
Feature 1                                           #                             
3RAM_A    312 y--------GSTDTGNVShvVPTIHPHIKIGs-rNLVGHThRFREAAaSVHGDEALIKGAKIXALXGL 370 Staphylococcus aureus subsp. a...
NP_832917 316 rv-----giGSTDAGNVSqvVPTIHPYIKIGp-dDLIAHTnEFREAArSELGDKALITSAKALANTAY 377 Bacillus cereus ATCC 14579
CAG75864  318 ke-----giGSTDAGDIShaVPTAHPTIKIGp-dDLIGHTvPFREAAnSPLGYAALLTGAQVLARTGL 379 Erwinia carotovora subsp. atro...
CAJ67613  315 sq-----giGSTDTGNLTheIPAIQAYIKLKe--NTATHTdEFAVAAgGEEGRVALIKAIKVLAMCGV 375 Clostridium difficile 630
CAJ69306  302 qv-----mlGSTDMGNLSqiMPCIHPLMKFSe-nGEELHTkEFLEASiNSYAKDRVIDGIKILALTGF 363 Clostridium difficile 630
CAJ69991  316 gp------vGSTDIGNVSqvCPTMYTEIALDispMVYVHEkEFLNYAnSEEAYDKLHKAVKAMVGCAL 377 Clostridium difficile 630
AAO36929  306 qk------tGSTDFGSVMyqVPGTCIRIAFVe-eGTSSHSeEYLKAGkTDEAHNAIMLSAKILADSAY 366 Clostridium tetani E88
CAC11380  295 vhrtneaamGSTDEANIShiIPTGHIDVKIVq-pSIPAHTdEFRMAAdPDKAADPLMTAIEATASAAA 361 Thermoplasma acidophilum
EDK87675  305 er------tGSTDFGNVMykVPGSCIRIAFVp-lGTSSHSdTFIECGkNEDAHKAILLASKILADSVY 365 Fusobacterium nucleatum subsp....
CBE68518  308 dg-----etGSTDVGNVSqvVPTIHPTIALTdhvDVVCHSaAFAQAAgGPDGDRAMITAAKALAMTAV 370 NC10 bacterium 'Dutch sediment'

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