2GEJ,2IW1


Conserved Protein Domain Family
GT4_PimA-like

?
cd03801: GT4_PimA-like 
Click on image for an interactive view with Cn3D
phosphatidyl-myo-inositol mannosyltransferase
This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
Statistics
?
PSSM-Id: 340831
View PSSM: cd03801
Aligned: 204 rows
Threshold Bit Score: 70.6445
Threshold Setting Gi: 123440914
Created: 6-Dec-2005
Updated: 9-Jun-2017
Structure
?
Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2GEJ_A        22 RIGMVCPYSFDv------pGGVQSHVLQLAEVLrd--aGHEVSVLAPASPhvklpdyvvsggkavpi---------pyng 84
gi 55980856    2 RVLFLSDARRI--------GGSEVYLREMLPRVka--lGLSPEAALPEAEgtl--------------------------- 44
gi 15805892    9 NLTVITDAPRV--------AGSEVWLLDVLPRLra--hGQRATVYCSAAPald--------------------------- 51
gi 8472177     6 DVEVIAPNFKQr------lSGVTSTIIQLVPVQra--lGQKIAVLGPGLP------------------------------ 47
gi 161330540   5 NILIISNYYPPfh-----iGGYELACRDTVEFLtk--kGHKVTVLTGNKGngaqsnieqmgavhreltyidydnpsyrqr 77
gi 15889463    8 DVQVLAPNFKRr------lSGVTSTIVQLIPVQnr--lGQKVGTIGPGLPphlphvrfrdlwrlw-------------qn 66
gi 83594676   19 PGVIFTMRYPSd-------VGFVWKTIALHRDLiarafGGEARCILAYPQlsptsayqprwstaiq-----------adf 80
gi 83590212    2 RVAMLHWAFPPi------iGGVESHLALLCPYLvr--qGHQVSLLTATAPgtpveeswqgvvikrspll-----dlnslt 68
gi 157059893   2 KILLVAEKYPPi------vGGGETHLHQLAEGLvt--rGHEVTVATEAVPdgperrlyregavrvhe--------vtglm 65
gi 83590494    5 KVALLTTNFFHpsseriimGGAERYQVDLCRLLke--lGYYVEVWQIGSGwt---------------------------- 54
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2GEJ_A        85 svarlrfgpathRKVKKWIaeg-----------dfDVLHIHEPnapslsm----------------lalqaaEGPIVATF 137
gi 55980856   45 ---------pvrRALEAAGipvhayrdlealpeavDLVVASAWypqsyr------------------rffarYPRLVLLV 97
gi 15805892   52 ---------rwaAQLTEQGvtve---------rwtDLLTLPEMtgdadlrvlqvw-----npdtyrtllpqlRAPRSVVL 108
gi 8472177    48 ------------KSLPSVRf--------------rDLIHLWKRpegrpcrvwharrnvemlpaillrdllrmKLRIVFTS 101
gi 161330540  78 aevetgnyqvcqGYINQLSp---------------DLIYLWNQqy-------------------------lsLAPSLASQ 117
gi 15889463   67 gpsggpriwharRNLEMLPgif-----------mrDVLRMKVKl---------------------------lFTSAAQRR 108
gi 83594676   81 ydysaenrqrleRVITDEAirlvvfm--saqpadvDLGFLRGL-----------------------------GLRTVTSE 129
gi 83590212   69 paviearageikELLENFLlav-----------rpDVVHAHNFhyfsyvha-------------aslqeicrRHGWPLVL 124
gi 157059893  66 dacqrldakaavASLHSVLttg-----------daDLVHVFNYvpallt------------------swlrpAIGAKLAV 116
gi 83590494   55 ----------reFDGVRIRsipvtks---eyntfpDLATAFYEnsmafdyal-----------yfiltlaypIAREKSIA 110
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2GEJ_A       138 Htsttksl--------tlsvfqgILRPYHEki-----------------iGRIAVSDLARRWQMEALgs---dAVEIPNG 189
gi 55980856   98 Hdqievfypl-----ggrylyrlGYRLLQVpnlr-------------rarAVLTVSRWAARWLERVHgv--kgVHAVPNG 157
gi 15805892  109 HdqldyhygwplqglyreiyrqtKAKFLPQa------------------eQRLTVSRWGAAFLQQELrv--sdVRAVPNG 168
gi 8472177   102 Asqrr--------------htgwSKFLIRRm------------------dAVIATSGRTAAYLDVPN------TVILHGI 143
gi 161330540 118 Nsgiaklyei----gdfwpaayiRPGFINAlkrkvkpwlpgliggrfdfgPCIACSHWVGKAIKEQLna--rnVAVIHNG 191
gi 15889463  109 Hsa-------------------yTRFLISKm-------------------DAVVATSTRSGSFLEVP------HRVVMHG 144
gi 83594676  130 Nnsydhslrqp---fmkrlakalIRRVLKRqv----------------fdLHIANSQAQYDFLRGFAqlpasrLRAVRNG 190
gi 83590212  125 Tahnvwd-----------delwtRMNSLARgw-----------------dLVIAVSHYIRQELVVNGypp-erVTVVYHG 175
gi 157059893 117 Slfethmpgqr---vfdlwgdypLERSLQRglvdn-----------lradVMVCGSQAYRRWALDAGftd-ddIHVVEFG 181
gi 83590494  111 Ishgvfwdwpg--fdlmagkpedRQEWLRRlsial-----------agpqKLVSVDTNTINYFNATLagfyhkWEYIPNY 177
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2GEJ_A       190 Vdvasfad--------aplldgyPREGRTVLFLGRYdePRKGMAVLLAALPKLVarf-pdvEILIVGR---------GDE 251
gi 55980856  158 Vdverfrpplp--gekealkgryGLKRPAVLVPARMs-PEKNHLAVLLTARLLPq------VDFLLVG---------TGE 219
gi 15805892  169 Vdtnrfsplpe--sareklraelGFRRFTVLVPGRFa-PEKNQLAAVWAARQASd------LDFVFVGdmd----sgGGR 235
gi 8472177   144 Dtkrfqppfdk---teakkalglDPAKKFVGCFGRVr-HQKGTDLFVDSMIALLpcr--pdWGAIVAGratgphlafESE 217
gi 161330540 192 Tqlgae-------------pqrnRKPPTSFLFCGRLc-QIKGIEQSLLAFTLLLakfpnrqLSLTCIGp-------iESR 250
gi 15889463  145 Vdtelfhpatg---pedtiaatgLPGHYLLGCFGRVr-HQKGTDLFVRAMIELLphy--pqWTAVVSGrvtaehkafGDT 218
gi 83594676  191 Vdtdhfqpge----rtgacstlgLDPDRLWIMAASQarPEKRLERVLEVIRAVKdarpalpIGFFFVG---------EGE 257
gi 83590212  176 TdtntfrppspedrqalytsypeWRGRRIIFHPARMs-RAKGCDVSIRALDLIRrei--pdVLLVLAGttnt--vdwGQK 250
gi 157059893 182 Tdldrfrddgaa-rarwrnhhgvADDETVFLVPARPv-PRKCIEDAVAALAHLAgrh--prARLVLTTptar-tldsYAE 256
gi 83590494  178 Vdtdlfsp---------paeepaGDDTVRVLFPRRLv-PVRGINETMRAAEKLTsry--pwIEFHFCGr-------gHDD 238
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
2GEJ_A       252 DELREqa-------gdLAGHLRFLGQVd-dATKASAMRsaDVYCAPHLGgESFGIVLVEAMAAGTAVVASDlDAFRRVLA 323
gi 55980856  220 LLGLWrraa----rllGLENVRFLGRR---EDMPELYRavDAVLLPTLG-ENQSLATLEAMASGLPVVTTPiPAQAELIQ 291
gi 15805892  236 LAQQLa---------rTQPNVQFWGRR---WDMPDLYRaaDVLLQPTLA-ENQSLVTLEALASGLPLVTTPiPAQRELVQ 302
gi 8472177   218 LKERVak-------agLADRILFVGEH---TNIPDWYRalDLFVAPQRW-EGFGLTPLEAMATGVPVVATDvGAFSELVT 286
gi 161330540 251 YKQVIeqmi---hnahLGEHVQILPPT---DDMKHVYQqhHVLLMPTLMpEPFGLVIIEAMANGLAVIASDrFGPAEIIN 324
gi 15889463  219 LKADVaa-------agLTDRIIFQGEV---DDIKPWYRrlTLYVAPSRN-EGFGLTPLEAMASETAVVASNaGAYEEMIV 287
gi 83594676  258 NLAEWrqia---lglpDAETYRFFGRQ---NDLRPYYQaaSVFIHGAFR-ESFGLVIAEAMASGLPVVATHaHGPAEIIA 330
gi 83590212  251 QPAEVaslqdliaslgLEENVFIRFFPw--QEMPAVYQgaEVCLYPSAFqEPFGLVMLEAMATARPIIVSRaGGMPEIIR 328
gi 157059893 257 KLRALade------lgVADRVIWEQGLs-wHDMPMLYAgaDAVVLPSSH-EGFGIALVEGMAGRRPVITSNvEGHDEVID 328
gi 83590494  239 NAERLmn-------qwAGNRERCFYYWkplEMMPEIYRqaDIVLIPSRStEGTSLAALEAMACGKPVIAGLaGGLSDIIL 311
                        410       420       430       440       450       460
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
2GEJ_A       324 d-gDAGRLVPVDd-aDGMAAALIGi----LEDDQLRAGYVAras-ervHRYDWSVVSAQIMRVYE 381
gi 55980856  292 d--GLTGRLVPPh-pRRLAQALRE-----ASPELGKRARAFv-----eATHTLDQAARRLAATLK 343
gi 15805892  303 d--GQTGLLVPPq-pALLARALRAl----AAHPEQARRYGAaarewvlDHHSLDQTVGQVAQAFA 360
gi 8472177   287 ggsEETGLIIAAddlKAMVDAAAAf----MDDRPRLAAASAnglartsKNFAIEQEARAIAAVYE 347
gi 161330540 325 d--DKLGILIDPedpIAIADAMAQl----IDNPARYHDITNagyqhvdAHFRLESVKAKVEQVLL 383
gi 15889463  288 t--GETGWVVGAgdyASLRDAIKTy----LADPALAKAHASaglahvrSTFPLEKEATCLGEVYE 346
gi 83594676  331 e-gETGRLVGRDd-wEGLGKAVIEy----LDDAELRRTHGLagrarcvALFSITRQAQEFADLLR 389
gi 83590212  329 p--GYNGFLVSMgdhEELARYTTFl----LRNPEVARTMGQdgrrlveENFTTAVMARNTLEAYN 387
gi 157059893 329 h--ERTGFLYPArdvEALAGCMARvitsdLENLVAAAHAEAv------RRFSSTAVAAGHERAYE 385
gi 83590494  312 h--GYNGYLIKPt-vENLVAAIEEl----ARDEGKRKLMGRrar-evaLTFNRKIWAERWARVLE 368
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap