3DUP


Conserved Protein Domain Family
NUDIX_Tnr3_like

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cd03676: NUDIX_Tnr3_like 
thiamine diphosphokinase Tnr3 from Schizosaccharomyces pombe and similar proteins
Tnr3 is a bifunctional enzyme composed of a C-terminal thiamine pyrophosphokinase domain, which transfers pyrophosphate from ATP to thiamine and an N-terminal NUDIX hydrolase domain that converts oxidized derivatives of thiamine diphosphate (oxothiamine and oxythiamine) to their respective monophosphates. Members of the NUDIX hydrolase superfamily catalyze the hydrolysis of NUcleoside DIphosphates linked to other moieties, X. Enzymes belong to this superfamily requires a divalent cation, such as Mg2+ or Mn2+, for their activity and contain a highly conserved 23-residue NUDIX motif (GX5EX7REUXEEXGU, where U = I, L or V), which functions as a metal binding and catalytic site. Substrates of NUDIX hydrolases include intact and oxidatively damaged nucleoside triphosphates, dinucleoside polyphosphates, nucleotide-sugars and dinucleotide enzymes. These substrates are metabolites or cell signaling molecules that require regulation during different stages of the cell cycle or during periods of stress. In general, the role of the NUDIX hydrolase is to sanitize the nucleotide pools and to maintain cell viability, thereby serving as surveillance & "house-cleaning" enzymes. Substrate specificity is used to define families within the superfamily. Differences in substrate specificity are determined by the N-terminal extension or by residues in variable loop regions. Mechanistically, substrate hydrolysis occurs by a nucleophilic substitution reaction, with variation in the numbers and roles of divalent cations required.
Statistics
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PSSM-Id: 467544
Aligned: 103 rows
Threshold Bit Score: 133.005
Created: 25-Apr-2006
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:NUDIX motif [structural motif]
Evidence:
  • Comment:G[X5]E[X7]REUXEEXGU, where U is a bulky aliphatic residue (usually Ile, Leu, or Val).
  • Comment:The NUDIX motif contains many of the active site residues of NUDIX hydrolases.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                   #######################           
3DUP_A    113 TFGVRAYGVHLNGYVGAga--dLHLWIGRRSPDKSVAPGKLDNXVAGGQPADLSLRQNLIKECAEEADLPEalaRQAIPv 190 Rhodospirillum rub...
CAQ69950  138 FLGLLTFASHMNGIVDGaaggqPALWISRRSPAKAIDPGMWDNLVAGGMPHGSDPLATLVRECEEESGIPPalaRGVQAh 217 Cupriavidus taiwan...
CBY07595  129 VLGIESYGVHCNVFKNDhe-gaKKLWIGHRSMDKTTFPGKLDTAVSGGMIYGLTVQENLEKEAEEEADVSPlasNTSKFi 207 Oikopleura dioica
ABE28887  110 FFGTMTYAVHLNGVVEYad-gaPQLWIARRSDTKATDPGMLDNVVAGGIGWGFGVEATIIKECWEEAGIPEeiaARAVA- 187 Burkholderia xenov...
EFE50647  119 PLGLYSHAVHINGLAVTee--gLHFWIARRSLHKAVDPNKLDTLVGGGISAGEQIWEAAKRESREEAGLTDavlNRLQYt 196 Neisseria elongata...
EIM31759   99 FLGLKTVGAHCNGFIVDhqgrpSHLWIGKRASGKATDPGLLDNLVGCGVPWPQTPDEAVIREGWEEAGLPAs-cMQQAL- 176 Leptothrix ochrace...
EGJ11710   96 YWGTLTLGAHATGWVAGpdgrpAALWVAQRSFTKSTDPGLHDNLIGGGVPIGQTPEETLVREAWEEAGLTPalmAARRP- 174 Rubrivivax benzoat...
EHR70403  117 FWGTLTFGAHANGYVAGpdgrpERLWVARRSWTKPTDPGALDNLVGGGVPHSQTPLEAVQREAWEEAGLTSahlHGLRP- 195 Burkholderiales ba...
EKF60320  106 ILGLWATKVHVNGLVNTgsdtpPDVWLSLRAAHSTAFPGYFDTLVAGGQPHDLDAATTAVKEAWEEAGIDAtlmENARHi 185 Agrobacterium albe...
EFV96168  112 PLGLLLRTVQVNVYTVHqg--qPRLWCARRAAHKAIDPGLLDSLVAGGIGCDETPLSTLFREAAEEAGLSQalaRHALPt 189 Lautropia mirabili...
Feature 1                                                                                     
3DUP_A    191 --GAITYCXEsp----agIKPDTLFLYDLALPedFRPHntDGEXADFXLWPaAKVVEAVRTteaFKFNVNLTVIDFAIRH 264 Rhodospirillum rub...
CAQ69950  218 --GMIEVLRDlp----egVQWEQVYVYDLLLPpgFIPHnqDGEVSEHRRVEvAPLLAIMSAg-aMTVDATLVTLDALGRR 290 Cupriavidus taiwan...
CBY07595  208 --KFARYCWHtd----fyIKREAIFIFDLDLSrfADPRpnDGEQSCFELCHlDDEIFNKPEk--WKPNSLAVSLDFAKRS 279 Oikopleura dioica
ABE28887  188 --GRTAHVLQslp---egTQAEQIFIYDLALPadFAPRnqDGEVGEHRLARiDEVARWIEEg-aMTVDASLATLDCLLRR 261 Burkholderia xenov...
EFE50647  197 -gRCISLHQVsr-----gLHRECLHIFDVILPneVIPQnqDGEVASFALMSaEEAAWAMCNg-eMMNDSVLVTADLFHRL 269 Neisseria elongata...
EIM31759  177 --LQNVYQVDrqd--aggQHRQRLYVYDLVLPreVKPQnqDGEVDAYFLLAiQEIVVDLKMa-rFTADAAITTLDFLQRH 251 Leptothrix ochrace...
EGJ11710  175 --GRVLRTARdip---egLQHELIHGWDLELPagVVPCnqDGEVHAFRLLDvEAALALAATa-aMTVDAALVTLDFAVRH 248 Rubrivivax benzoat...
EHR70403  196 --GRVLLVQRdip---egLQVEELHVFDLPLPadVQPRnqDGEVAELMCLPmDEALAATNE---MTVDAALATLDLALRH 267 Burkholderiales ba...
EKF60320  186 gdEPVCYVSRq------gFHQELLSVYDLVLPrdWSPTciDGEVESNTLVSmDELRSGLAGaldIKFGSHLVCQDVVTRH 259 Agrobacterium albe...
EFV96168  190 --GIMDSTSLseddghtvLHRERMHVFDLQVPegFQPThpDGETESASLQSlEAVLAQIRQg-qWTREGAWASINLMHRH 266 Lautropia mirabili...

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