1VDY


Conserved Protein Domain Family
ENTH_like_Tepsin

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cd03572: ENTH_like_Tepsin 
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Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and similar domains
This family is composed of proteins containing an ENTH-like domain including vertebrate AP-4 complex accessory subunit Tepsin and Arabidopsis thaliana VHS domain-containing protein At3g16270. Tepsin is also called ENTH Domain-containing protein 2 (ENTHD2), Epsin for AP-4, or Tetra-epsin. It associates with the adapter-like complex 4 (AP-4), a heterotetramer composed of two large adaptins (epsilon and beta), a medium adaptin (mu) and a small adaptin (sigma), which forms a non-clathrin coat on vesicles departing the Trans-Golgi Network. The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.
Statistics
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PSSM-Id: 340773
Aligned: 55 rows
Threshold Bit Score: 100.322
Created: 3-Mar-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:putative phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:based on the binding of Rattus norvegicus Epsin ENTH domain to inositol 1,4,5-trisphosphate
  • Comment:ENTH domains bind phosphoinositides (PtdInsPs), usually with a preference for PdtIns(4,5)P2, although not all ENTH domains show this preference.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #   ##                                  #   #    #                         
1VDY_A     12 RSRMIDAVTSDEDKVAPVYKLEEICDLLRsshVSIVKEFSEFILKRLDNKs-PIVKQKALRLIKYAVGKSg--------- 81  thale cress
EDQ49225   13 RAVLIDGATSDNDSVAPVYKLEEICTLMRtssLDIVRELVERILKRMDHKs-PIVKQKALRLIKFAAGKAg--------- 82  Physcomitrella patens
EFJ17215   13 RTRLIDGVTTDDDKVAPVYKLDEISSVLRsapHDVVHEMVEYVFKRLEHKs-PLVKQKTLRFIKFEVGKSg--------- 82  Selaginella moelle...
EEH52132   24 RHRKIDAVCVDDAKPAAVYLLEELATATRswpTDALDDVAAHVGKRIDHSs-PHVKLKALRCLKYVCQRGd--------- 93  Micromonas pusilla...
KDD72018   17 LRRKIDEAVLYRDDPPPVYLLDELVSMIGs-nPEQIEQAVDAIVKRLQSRg-ASAKQQTLRLVKHACQKGs--------- 85  Helicosporidium sp...
KFM22766   14 LQIKIAEATSSSDEVTPVYILDEVLSLVGs-nRDAIDTATNAIVPRLKARs-ATVKQKALRLTRHACLKGd--------- 82  Auxenochlorella pr...
EAY21877    4 CGRLVDQATQENDLPAPGAVLAESVQLCRk-dATNVNRIVTKTMEKLQKPs-VMIRLKALRYLLHLTQNGp--------- 72  Trichomonas vagina...
AGM32297    4 VPRLTDKATTNDDTPAPGQVLAESLELCRk-dSINVSRFLESCMKKLENQs-VQVRIKTLKFLLYLVQHGp--------- 72  Formosan subterran...
KCV68734   17 QHPSLLNATSEDSRPTPGPVLDEIAQLTWs-sAEEASDVIRFLYHRLNYSdsPFVLLKSLRCLRHVVLRGserahflarl 95  Fonticula alba
CEG01188    9 RRARFDRALVDDEDPTPVYTTEQLVALANtlrGDDARDACEHLARRAEHAs-PRVARKALKLIARCCDGArd-------- 79  Ostreococcus tauri
Feature 1                                                          
1VDY_A     82 --sEFRREMQRNSVAVRNLf-hYKGHPDPLKGDAlNKAVRETAHETISAIFSE 131 thale cress
EDQ49225   83 --vEFRREIQRQAPAVRNLl-hYRGAPDALKGDAlNKAVRETAQEALSAIFAS 132 Physcomitrella patens
EFJ17215   83 --iEFKREIQRQSAAIRQLf-hYQGQPDSLRGDSlNKAVRETAREAITAMFAS 132 Selaginella moellendorffii
EEH52132   94 --aRFRRAAAKHCAARVRAcvaHRGPPDEHRGDApHEAVRDQATEAIAAMFPK 144 Micromonas pusilla CCMP1545
KDD72018   86 --pNLRLALVRQSAAVKELe-hYCCEPDPFKGDTpWKKVQMLAREALEAMHSS 135 Helicosporidium sp. ATCC 50920
KFM22766   83 --aALRSRLTHLASPAVRDllsYRCDPDPFKGDIpWKRVQELAKDTLTAMHAG 133 Auxenochlorella protothecoides
EAY21877   73 --pSVQNELRLYSAQISQCm-sWRGLPHPTRQWEpYQEMNDVAQSLLDLVFAA 122 Trichomonas vaginalis G3
AGM32297   73 --pSFGTELKFYSANLSGCm-gWRGAPHPTKGYEaYTEMKDLAQQVLDLCYLD 122 Formosan subterranean termite
KCV68734   96 pdgHITLALKRVAHFHNPIn--VANLPKAQLDVL-VKAVRDTAIEVTKLVFED 145 Fonticula alba
CEG01188   80 --gTFTREMSKLSTRIRALq-sRVGAPHPSKGDSeHAAVRAAAREAMGAIFGG 129 Ostreococcus tauri

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