1H0A,3ONK,4GZC,2QY7,5J08


Conserved Protein Domain Family
ENTH

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cd03571: ENTH 
Click on image for an interactive view with Cn3D
Epsin N-Terminal Homology (ENTH) domain family
The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, contributing to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.
Statistics
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PSSM-Id: 340772
View PSSM: cd03571
Aligned: 87 rows
Threshold Bit Score: 84.8765
Threshold Setting Gi: 1073549082
Created: 2-Mar-2006
Updated: 9-Jun-2017
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:1H0A; Rattus norvegicus Epsin ENTH domain binds inositol 1,4,5-trisphosphate or Ins(1,4,5)p3; contacts at 4.0A
    View structure with Cn3D
  • Comment:ENTH domains bind phosphoinositides (PtdInsPs), usually with a preference for PdtIns(4,5)P2, although not all ENTH domains show this preference.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #   ##                                         #                #   #      
1H0A_A        20 AEIKVREATSNdpwGPSSSLMSEIADLTYnv---------vAFSEIMSMIWKRLNdh-----------gKNWRHVYKAMT 79
gi 678345830   4 YQKVLKECTSNenwNISNSKLQVLADHSYnw---------nDYTVIMQHLWKKMNsk-----------pKEWRRIFKALH 63
gi 124414885  22 LEQTLSEATSNenwNTPTKLLQEVAEASYgy---------tSCDTIMKFIWKRLDsd-----------nKEWRRILKTLN 81
gi 574999439  24 FEKNLKEALNNknyGVSNSLLYDLSISTYdv---------nYYKRVMTEVFKAIQek-----------pTRWRRIYKGLK 83
gi 586728365  23 LEKLLHEVTSNdnwGVNPKLLRQVSDESYhf---------eNREVILNFIFHRLSnk-----------tKNWRKILKTLE 82
gi 403331097  29 IDKILIEATGNenwNIANSKLQILSEACYhh---------dQCQKILDHLKSKLDsp-----------vFEWRRILKSLH 88
gi 678331669  29 IDKLLVEATANenwNIANSKLQILADACNspqllvlknnsdDCKVILDYLKYKLEvp-----------aYEWRRIMKSLS 97
gi 678321403  14 IEKILIEATGNenwNIANSKLQMLADAAHqh---------dNYQRIMDHLKGKLEcpafewrrvlkvskQKCLISMQSLV 84
gi 399215961  18 LDKLLKEALYDksvGCIDSILNGIAQASHhm---------pFYEIISKRTWSAINsk-----------pSKWLYIQKGLT 77
gi 656184784  19 VDKRIKEALYSndvGCPETVLYELSQATYha---------aFMERISQATWSCLKas-----------nARARRIQKALT 78
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1                                                                            
1H0A_A        80 LMEYLIKTGSERVSQQCKENMYAVqt--lkDFQYVDRDGk---------dQGVNVREKAKQLVALLRD 136
gi 678345830  64 AMEYLIKNGAPRCIQEIKDDLFKIra--lqDFSYSENGTd----------RGQGVRDKSRSLCELLSD 119
gi 124414885  82 MIEYLTKNGAPRCVGEFRDNIYKIrs--fsDFFLVEQGSd----------KGLSIRDKTKQLVDLLSN 137
gi 574999439  84 LCEYVMKNGCEYFISDVKDKEELIkk--ltHFTHLEDLKd----------KGIGIRDISNNILKLLKD 139
gi 586728365  83 LIEYLIKNGLYDLCSDFRREIVHItt--hcDFQMLEKGVd----------RGLSIRNKAQKIVQLLSD 138
gi 403331097  89 ALEYIIKNGPPRLQGDLKYEMYKInn--lqNFTHFEEGAd----------KGSSIRQKSHQICDLLTN 144
gi 678331669  98 AMEYLLKNGPFRVQQDLRNEMFKVtt--lqNFTYYEDNId----------KGSSIREKAILLADLLTS 153
gi 678321403  85 ALEYILKHGPARVQQDLRNEMFRLnk--lqQFTHFEDGSd----------KGNSIRQKAHLIADLVTI 140
gi 399215961  78 LLEYLVLNGAEWCISDVLQHLEDInkvvklNREYSAKTSfltgkplapphTAFVITEKCKNLINLVRM 145
gi 656184784  79 LLTYLALNGAEGCASDILTRLDDIte--lhDRKLPEHMRd----------LEIIIKDKAINLVSLVCD 134

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