1X51,1RRQ


Conserved Protein Domain Family
DNA_Glycosylase_C

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cd03431: DNA_Glycosylase_C 
Click on image for an interactive view with Cn3D
DNA glycosylase (MutY in bacteria and hMYH in humans) is responsible for repairing misread A*oxoG residues to C*G by removing the inappropriately paired adenine base from the DNA backbone. It belongs to the Nudix hydrolase superfamily and is important for the repair of various genotoxic lesions. Enzymes belonging to this superfamily requires a divalent cation, such as Mg2+ or Mn2+ for their activity. They are also recognized by a highly conserved 23-residue nudix motif (GX5EX7REUXEEXGU, where U = I, L or V). However, DNA glycosylase does not seem to contain this signature motif. DNA glycosylase consists of 2 domains: the N-terminal domain contains the catalytic properties of the enzyme and the C-terminal domain affects substrate (oxoG) binding and enzymatic turnover. The C-terminal domain is highly similar to MutT, based on secondary structure and topology, despite low sequence identity. MutT sanitizes the nucleotide precursor pool by hydrolyzing oxo-dGTP to oxo-dGMO and inorganic pyrophosphate. The similarity strongly suggests that the two proteins share a common evolutionary origin.
Statistics
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PSSM-Id: 239523
View PSSM: cd03431
Aligned: 88 rows
Threshold Bit Score: 43.8647
Threshold Setting Gi: 16588988
Created: 13-Sep-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
DNA binding and
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:DNA binding and oxoG recognition site [nucleic acid binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       ###                                             
1X51_A       17 REESSATCVLEQpga----lgAQILLVQRPNSGLLagLWEFPSVTWeps------eqlQRKALLQELqrwagplp----- 81
1RRQ_A      238 KQVPLAVAVLADd-------eGRVLIRKRDSTGLLanLWEFPSCETdg--------adGKEKLEQMVgeqyglq------ 296
gi 29834502 235 VTLFRWVAIVLYe--------DSIVLEQRKPEEMMagLYEFPYIEVesfd-----dfsDIEGLIQEMgervgtp------ 295
gi 15835935 227 VRHARKKVIFLHrlvaivlydGSLVVEKRRPKEMMagLYEFPYIEVe-----------PEEGLQDIEgftkkmelslesp 295
gi 89517884 224 KNLHHHYIVIQTp-------kAKTLLQQRPQSGIWagLFEFPFVESdgal----lsveFREKLKQQDwwsgfrf------ 286
gi 46399364 227 QYLYRAVAIVKCg--------DYFLVKKGKKGEIMsdLYEFPFFEVsqegitfkelqeNIKLHWNLTvhll--------- 289
gi 50877352 235 IHIRRVAIFQVSe--------GRLLIQPSRHPTLWqgLWEFPHTDLqeee----avevGITRLLGLLpsenr-------- 294
gi 21672797 229 LEKKSWFVVIKYq--------NQFWIEKNTEKKIWknLFCFPNFDTki---------kTIEWLKKNKinidk-------- 283
gi 87308869 232 EDRTEVAIIVRRk--------KEVLLRQCGADERWagLWDFPRFQVaseae--llsgeLASQLEERTglsaa-------- 293
gi 33152975 226 PERQTYFLVVKSg--------TRILLQQRAAKGLWggLYSFPQFEDf----------aTIKRTISAKnwqi--------- 278
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  #  ##                                                                
1X51_A       82 athlrhlGEVVHTFSHIKLTYQVYGLAlegqtp----------------vttvppGARWLT---QEEFHtaavstAMKKV 142
1RRQ_A      297 veltepiVSFEHAFSHLVWQLTVFPGRlvhgg-------------------pveePYRLAP---EDELKayafpvSHQRV 354
gi 29834502 296 lvfhgeiEEQRHAFTHYKVRLIPRIFRakf----------------------kpkPELLYP---LKGLDslpfssGHRKI 350
gi 15835935 296 leflgnlKEQRHAFTNHKVHLCPIIFKats----------------------lpqFGELHL---LSDIDhlafssGHKKI 350
gi 89517884 287 resvynqDPIIHKLSHRKIHAYFWIVEldv-----------------------elKDSIDI---KEAFQkpl-hiLMHRF 339
gi 46399364 290 ----qslSSIEHGFTRYQARLDPCVFNclem--------------------spvkDLEWLD---TKSLKqkafssGHRRI 342
gi 50877352 295 --vlepmVTVKHAYTKYRVQFHCYLLRgeag--------------------cqgeEQRLCD---WQALArhgfsaGPRKV 349
gi 21672797 284 --khkkiQSFYHKFSHFTLHIIPILVNlsffkn----------------fqnskkTGIWYDlknTHDIGlp---kPVQKI 342
gi 87308869 294 --igsrlTTIKHGVTKYRITLHCHEAEgmtgrl----------------rkdataPLVWAA---VDKLAdfalstTGRKI 352
gi 33152975 279 ---sqqlITFRHTFSHFYLDITPILVDleaqdsvqmpplvaqepvsdycqsvsssENYWYD---LHATNevglaaPVKKI 352

                ....
Feature 1           
1X51_A      143 FRVY 146
1RRQ_A      355 WREY 358
gi 29834502 351 KAWL 354
gi 15835935 351 KDAL 354
gi 89517884 340 MSSF 343
gi 46399364 343 FQSL 346
gi 50877352 350 LEHI 353
gi 21672797 343 LEIF 346
gi 87308869 353 AQLL 356
gi 33152975 353 LDEL 356

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