1G9Q,1KHZ,1MQW,1V8R,1VHZ,1VIU


Conserved Protein Domain Family
ADPRase_NUDT5

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cd03424: ADPRase_NUDT5 
Click on image for an interactive view with Cn3D
ADP-ribose pyrophosphatase (ADPRase) catalyzes the hydrolysis of ADP-ribose and a variety of additional ADP-sugar conjugates to AMP and ribose-5-phosphate. Like other members of the Nudix hydrolase superfamily, it requires a divalent cation, such as Mg2+, for its activity. It also contains a highly conserved 23-residue Nudix motif (GX5EX7REUXEEXGU, where U = I, L or V) which functions as a metal binding site/catalytic site. In addition to the Nudix motif, there are additional conserved amino acid residues, distal from the signature sequence, that correlate with substrate specificity. In humans, there are four distinct ADPRase activities, three putative cytosolic enzymes (ADPRase-I, -II, and -Mn) and a single mitochondrial enzyme (ADPRase-m). Human ADPRase-II is also referred to as NUDT5. It lacks the N-terminal target sequence unique to mitochondrial ADPRase. The different cytosolic types are distinguished by their specificities for substrate and specific requirement for metal ions. NUDT5 forms a homodimer.
Statistics
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PSSM-Id: 239516
View PSSM: cd03424
Aligned: 275 rows
Threshold Bit Score: 67.1297
Threshold Setting Gi: 46201406
Created: 12-Sep-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1MQW; M. tuberculosis ADPRase binds AMPCPR and 3Mn2+; defined at 3.5A contacts.
    View structure with Cn3D
  • Structure:1KHZ_B; Escherichia coli ADPRase binds AMPCPR and 3Mg2+; defined at 3.5A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                ###                           #   # 
1G9Q_A       56 RGHAAVLLPFDpv----rdEVVLIEQIRIaaydtsetPWLLEMVAGMIEe--------------GESVEDVARREAIEEA 117
1MQW_A       42 HFGAVAIVAMDdn-----gNIPMVYQYRHty-----gRRLWELPAGLLDv-------------aGEPPHLTAARELREEV 98
gi 9107123   68 GGLAAIILAVTpe-----eRIIFVEQFRIpi-----qQRTIEMPAGLVGdi-----------daGESIEQAAIRELEEET 126
gi 56551937  39 NMGAAVILALDg------eYVILVEQPRIpf-----gAHTIELPAGLIGdt-----------dsHESVETAAARELIEET 96
gi 66811942  48 DTDGVDILATVkkd--gkkYLIVVVQYRVpv-----dNLVIEFPAGLVDn--------------DENFVNSAIRELKEET 106
gi 68538201  35 GIHAAVILAIDeda--rgrHAILVEQYRVpl-----kRQCLELPAGLVGdd-----------saGEAAEVAAARELEEET 96
gi 87198898  37 NIKAAVILAIDd------gHVLLVEQFRVpl-----gRPCIELPAGLIGdea---------gaeNEDAATAASRELEEET 96
gi 88179317  63 HTPVGTLIPNIyknnqnqwCTILVQQFRPqy-----eSDTIEFPAGFINnktkdnnnnrtdadpVETAKQAAVRELEEET 137
gi 89287472  51 PYDGVATFAIIkkdn-qpsKLIIIANYRApv-----dKFVLEIPAGLMD---------------TPDLVQNAIRELKEET 109
gi 89292472  58 ECNGVDVCPIIkykd-grrKLLLIANYRPpv-----nAYTVEFPAGLLD---------------DGDLLENARRELKEET 116
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      #                          #                     
1G9Q_A      118 GLIVk------RTKPVLSFLAs----PGGTSERSSIMVGEvdattasg--ihgladenE-DIRVHVVSr-------EQAY 177
1MQW_A       99 GLQAs------TWQVLVDLDTa----PGFSDESVRVYLATglrevgr-----peahheEaDMTMGWYPi-------AEAA 156
gi 9107123  127 GWTAd-----hAGILLIGPVS-----SGASSERIAFVRATglrkvss-----gggdvdE-DIVVHEIPr-------TQAA 183
gi 56551937  97 GYQAd------IIENLGQFASs----PGMTSESFFLVRAQnltrise-----gggvddE-EITVHRVKl-------SELP 153
gi 66811942 107 GYRTtp----dKVLLTSPILPl---eCGMSCANARMVVIDleetice----eqelegsE-NIDIFYLPmdnlfnelEELR 174
gi 68538201  97 GYRAa------TWRTVGEFYSs----PGMVSESFTLLVATgltkvga-----gggvggE-DIVVHRVPl-------NGIE 153
gi 87198898  97 GYRPg------RIESLGEFHSs----PGMVTEAFTLFRAYdlekvse-----gggvegE-GITVHRVAl-------TEIE 153
gi 88179317 138 TKTGt------VLSTSPPLTSg----PVPVAARLAAVLVHard------------kegE-EAGRQRLDegefavevQETT 194
gi 89287472 110 GYTAeeqplkvSNKESPPTYTd----PWRSCSCTKSVFLEvngnkqeninpkqdledaE-SIFVHLIDide--nitDTLT 182
gi 89292472 117 GYTA-------DEIKVLDYCPllrtdPWKSNETTKVVIAYidgdkecninpkqdleteE-NIDIFTVDl------nEKFM 182
                       170       180
                ....*....|....*....|....*...
Feature 1                                   
1G9Q_A      178 QWVEegk-----idnAASVIALQWLQLH 200
1MQW_A      157 RRVLrge-----ivnSIAIAGVLAVHAV 179
gi 9107123  184 AWLVqkmha-gfamdPKLWAALWLIDHA 210
gi 56551937 154 DFLEqk-------rqQGLVIDAKLLLLL 174
gi 66811942 175 KKLNcv-------vsIQLYTFALGLNFN 195
gi 68538201 154 DFVAakrae-gcgidVRVAMLLAGGLLA 180
gi 87198898 154 SFLSar-------raEGYAIDVRMLLLL 174
gi 88179317 195 RVATrttklkitiplDTSWIVFKGCNES 222
gi 89287472 183 TLAEkhgy----iisSTLWCFAQGIQFA 206
gi 89292472 183 ENLRkivee--nnyqMTLQMYSLCLGFS 208

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