Conserved Protein Domain Family
GST_C_EF1Bgamma_like

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cd03181: GST_C_EF1Bgamma_like 
Click on image for an interactive view with Cn3D
Glutathione S-transferase C-terminal-like, alpha helical domain of the Gamma subunit of Elongation Factor 1B and similar proteins
Glutathione S-transferase (GST) C-terminal domain family, Gamma subunit of Elongation Factor 1B (EF1Bgamma) subfamily; EF1Bgamma is part of the eukaryotic translation elongation factor-1 (EF1) complex which plays a central role in the elongation cycle during protein biosynthesis. EF1 consists of two functionally distinct units, EF1A and EF1B. EF1A catalyzes the GTP-dependent binding of aminoacyl-tRNA to the ribosomal A site concomitant with the hydrolysis of GTP. The resulting inactive EF1A:GDP complex is recycled to the active GTP form by the guanine-nucleotide exchange factor EF1B, a complex composed of at least two subunits, alpha and gamma. Metazoan EFB1 contain a third subunit, beta. The EF1B gamma subunit contains a GST fold consisting of an N-terminal thioredoxin-fold domain and a C-terminal alpha helical domain. The GST-like domain of EF1Bgamma is believed to mediate the dimerization of the EF1 complex, which in yeast is a dimer of the heterotrimer EF1A:EF1Balpha:EF1Bgamma. In addition to its role in protein biosynthesis, EF1Bgamma may also display other functions. The recombinant rice protein has been shown to possess GSH conjugating activity. The yeast EF1Bgamma binds to membranes in a calcium dependent manner and is also part of a complex that binds to the msrA (methionine sulfoxide reductase) promoter suggesting a function in the regulation of its gene expression. Also included in this subfamily is the GST_C-like domain at the N-terminus of human valyl-tRNA synthetase (ValRS) and its homologs. Metazoan ValRS forms a stable complex with Elongation Factor-1H (EF-1H), and together, they catalyze consecutive steps in protein biosynthesis, tRNA aminoacylation and its transfer to EF.
Statistics
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PSSM-Id: 198290
View PSSM: cd03181
Aligned: 83 rows
Threshold Bit Score: 103.795
Threshold Setting Gi: 71665123
Created: 10-Nov-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
N-terminalputative dimer
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:N-terminal domain interface [polypeptide binding site]
Evidence:
  • Structure:1NHY; Saccharomyces cerevisiae EF1Bgamma; contacts at 3.5A between N- and C-terminal domains
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    #                                                                  #
1NHY_A        92 AQAQIIRWQSLANSDLCIQIANTIvplkgg-apynkkSVDSAXDAVDKIVDIFENRlk---nYTYLATeNISLADLVAAS 167
gi 71013430   93 SAALVEQWISFADDEILQFGVQLMlickgl-lpynkaYEQKLWENLDRSFTYLEAElk---kKTFIVGhRVTLADLTLAS 168
gi 3650373    92 EEAEMLKWMCFINFDIVTPQNVRPwvgmfr--gnipyEEKPFKESATRAIDSLKIPnelvkdRTYLVGdRFTLADLFFGS 169
gi 302677598  91 DAALVDQWTHFADSEIDAVQLIIFnmvkglgiaynklVHTALLERVHRAYTTLNNYla---tRTFLVGeRITLADIWVAA 167
gi 729399     89 EKAKVLQYCSFTNSELPGAFRPIIaprvfg-apydeqAAKEAETAIALIFARFDEEla---sKTYLVGsRLTLADIFFTC 164
gi 213404932  89 ARAKALQWASFANSELIRAFAQWMlgrhgi-lpynaaAEKESKQQTFALFDFLNKEla---dKTYLAGdRLTIGDIFVAS 164
gi 145528197  88 QQSQVDQWIDWTINELDPNFMTTFpqlwgh-ypanedTFKTAKNIINDKLKQLEGHfk---nSPYLVGdKLTIADVTLIV 163
gi 145507520  85 QQAKVRQWLDFTSLELEPTLLGLLlpilgy-eqttpqKQEEVKKELNWKLKILENHfk---aNNFLVGeSLTIADLNLAS 160
gi 68128987   95 EASQVDMWIDFSSTEIDAANMPYIrmaye---kaisdVPADTLDKVKAVLSALEEVls---vRTFLVGeRLTIADVAVAF 168
gi 74025620  221 EGSQVDMWLDFAATELDTAAKPFGlniy-----rgvpIPVDAMDSVHEVFGGLETWle---tRTFLVGeRLTVADICVAF 292
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1           #                                        #  ###              
1NHY_A       168 IFTRYFESLFGte--wRAQHPAIVRWFNTVRASP----------FLKDEYk-----DFKFADKP 214
gi 71013430  169 DIGFVFGHVAGvn--fRSKYPNTVRYFNTVTKQPt---------ALKAYS------SLTLAEDN 215
gi 3650373   170 LLRIFFNSIIDek--tRKELPHLTRYYITMFHQA----------KLETYFp----lELPLTVTV 217
gi 302677598 168 ITQFGTFFHFDna--tRTKFPHLIRLVDTIVNQP----------KLAGTFsatpslEKPLAFTP 219
gi 729399    165 FLKFGATYVLTks--yLAKYTHIYRYYQTIYHQA----------KLDAITe-----PLKFIDQP 211
gi 213404932 165 FLNALFRTVFTka--eLAKYGNVQRYFTTMYYQTgldslhgeltIVDTSI------PVPKVEKP 220
gi 145528197 164 RIAPFFILLIDek--tRKSYPSLMKWFTAVSELP----------QFKKNFg-----RVRLCKVA 210
gi 145507520 161 YFQGLFSFAFDkq--fRGTIPNISRWYKHISELP----------AFVDYYg-----KTKYCVKA 207
gi 68128987  169 SIHWVYRCNRKhgqtlYKEYRAVYRHYNTVMRHP----------KIHAMM------RKEGAAIG 216
gi 74025620  293 ALQFHYRANAAegealTKKYRNVYRLYNTVMQQP----------KTVEVLk-----SQGATFGP 341

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