1Q54,1I9A,1X83


Conserved Protein Domain Family
IPP_Isomerase

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cd02885: IPP_Isomerase 
Click on image for an interactive view with Cn3D
Isopentenyl diphosphate (IPP) isomerase, a member of the Nudix hydrolase superfamily, is a key enzyme in the isoprenoid biosynthetic pathway. Isoprenoids comprise a large family of natural products including sterols, carotenoids, dolichols and prenylated proteins. These compounds are synthesized from two precursors: isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). IPP isomerase catalyzes the interconversion of IPP and DMAPP by a stereoselective antarafacial transposition of hydrogen. The enzyme requires one Mn2+ or Mg2+ ion in its active site to fold into an active conformation and also contains the Nudix motif, a highly conserved 23-residue block (GX5EX7REUXEEXGU, where U = I, L or V), that functions as a metal binding and catalytic site. The metal binding site is present within the active site and plays structural and catalytical roles. IPP isomerase is well represented in several bacteria, archaebacteria and eukaryotes, including fungi, mammals and plants. Despite sequence variations (mainly at the N-terminus), the core structure is highly conserved.
Statistics
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PSSM-Id: 239218
View PSSM: cd02885
Aligned: 57 rows
Threshold Bit Score: 188.157
Threshold Setting Gi: 19068586
Created: 7-Oct-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1Q54: E. coli IPP isomerase binds inhibitor bromohydrin pyrophosphate, Mn2+, and Mg2+
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #   #      #   #              #   #           # #             
1Q54_B        3 TEHVILLNaqgvPTGTLEKYAAHTADTrlHLAFSSWLFNakgQLLVTRRALSKKAWPGVWTNSVAGHPQLGESNEDAVIR 82
gi 6226829   11 IERVVLLNdrgdATGVADKATVHTGDTplHLAFSSYVFDlhdQLLITRRAATKRTWPAVWTNSCCGHPLPGESLPGAIRR 90
gi 10567363   9 PEMVILCNelgeATGTAPKSEIHHSDTplHLAFSVYIFNgdnKLLVTRRASDKITWPGVLSNSCCGHPQPGEPLTEAIER 88
gi 10581266  29 TQDVIAVTpddeRTGLANRLDAHTGDGvrHRAFTCLLFDedgRVLLAQRADRKRLWDTHWDGTVASHPIEGQSQVDATRQ 108
gi 36786673   1 MENVVLVDendnRVGCINKIDAHLNGDhlHRAFSCFIINsknEVYIQQRAQSKLLWPGYWSNSYCSHPRPDEEISHAVMR 80
gi 67154000  39 DEPLVLVDahdrEIGFLDKASAHQGQGllHRAFSLFVFNrrgELLLQQRAAGKRLWPGFWSNTCCSHPRRGETLDEAVER 118
gi 68174208  33 ADEVVLLDaerrPCGTAPRIAVHGLDTplHLAFSSYLFDpsgRLLVTRRALDKRTWPGVWTNSCCGHPRPGEDLARAVER 112
gi 68227455  30 PELVILLSpsgdITGTTPKATVHGPQTplHLAFSSYVFDdmdRILVTRRALEKPTWPGVLTNTCCGHPAPGEDFREAISR 109
gi 84497359   6 DELVVLLGddgrPIGTAPKATVHHSETplHLAFSCYVFDpagRFLLTRRALTKKTWPGVWTNSCCGHPGPGEPVEEAVQR 85
gi 89342416   3 DEQVVLLDpdgsSVGTAAKSTVHHGDTplHLGFSCYLFDssgRVLLTRRALGKKTWPGVWTNSFCGHPAPGEDVTDAVHR 82
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1       #   #                   #         # #                                           
1Q54_B       83 RCRYELGVEI---TPPESiyPDFRYRATDPSGIVENEVCPVFAARTTSAlqi--nddEVMDYQWCDLADVLHGIdat-pw 156
gi 6226829   91 RLAAELGLTP---DRVDLilPGFRYRAAMADGTVENEICPVYRVQVDQQprp--nsdEVDAIRWLSWEQFVRDVtagvia 165
gi 10567363  89 RVHEELRIRA---DDIRLvlPEFSYRASMTNGITENELCPVFAAHCKTSgvnp-dlnEVSAWEWVHWQWFFESVqae-rl 163
gi 10581266 109 RLAEELGIEPhqyDKLEIt-DRFEYKRRYLDEGLEWEVCAVLQATLHDTsfdr-dpeEVGGAMWVDYEDLYENPryyrql 186
gi 36786673  81 RAEEELGIVIn-eEPKFL--YKFQYKEKYKDVGYEHELCHVFVVFTDTPpte--npkEVSAGFFINIKDVQEYIhnn-ee 154
gi 67154000 119 RLNEELSMQC---KLQFL--FKFEYQAQFDAEGAEHELCSVYAGRSDEApsl--nlnEVSALRYISPEALDAEMaeh-pq 190
gi 68174208 113 RVGQELRLEL---TDLHCalPDFAYRATAADGLVENEVCPVYVARALGDpdp--dpaEVVEWRWVEWDDYRRVAaaa-pw 186
gi 68227455 110 RLQEELGLQV---QRIDLalPAFRYRCVMDDGMVENEICPVFFTRASGPfri--npdEVAEAFWLPWSKFVDDVtsg-al 183
gi 84497359  86 RVRQELGTEL---TGIRLvlPDFRYRAIMDDGTVENELCPVYAATCPDPralvldpaEVAEVEWVDWAAFRRDVlvs-gr 161
gi 89342416  83 RAGRELGAAL---ADVVCvlPDFRYWARAADGTVENEICPVFCALIDGTvsp--apdEVMDLEWVEWSDLRSAAalr--w 155
                       170
                ....*....|.
Feature 1           #      
1Q54_B      157 aFSPWMVMQAT 167
gi 6226829  166 pVSPWCRSQLG 176
gi 10567363 164 lVSPWCRAQVD 174
gi 10581266 187 rLCPWFEIAMR 197
gi 36786673 155 fCTPWFKKEWA 165
gi 67154000 191 rFTPWFKIEWE 201
gi 68174208 187 aLSPWSVDQMA 197
gi 68227455 184 aISPWCRLQVE 194
gi 84497359 162 dVSPWCGLQVA 172
gi 89342416 156 aISPWAQSQVP 166

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