2WSK,2VNC


Conserved Protein Domain Family
E_set_GDE_Isoamylase_N

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cd02856: E_set_GDE_Isoamylase_N 
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N-terminal Early set domain associated with the catalytic domain of Glycogen debranching enzyme and bacterial isoamylase (also called glycogen 6-glucanohydrolase)
E or "early" set domains are associated with the catalytic domain of the glycogen debranching enzyme at the N-terminal end. Glycogen debranching enzymes have both 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities. As a transferase, it transfers a segment of the 1,4-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or another 1,4-alpha-D-glucan. As a glucosidase, it catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Bacterial isoamylases are also included in this subfamily. Isoamylase is one of the starch-debranching enzymes that catalyze the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans such as amylopectin or glycogen. Isoamylase contains a bound calcium ion, but this is not in the same position as the conserved calcium ion that has been reported in other alpha-amylase family enzymes. The N-terminal domain of glycogen debranching enzyme may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.
Statistics
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PSSM-Id: 199886
Aligned: 214 rows
Threshold Bit Score: 81.924
Created: 1-Jul-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2WSK_A        10 APLGAHYDGQGVNFTLFSAh-AERVELCVFDANGqe---hRYDLPGhs--------gdIWHGYLPDarpGLRYGYRVHGp 77  Escherichia col...
NP_875794     22 WPLGSSITKRGVNFSVAAPn-ASYVELLLFNNANdstpkeIVKLNSenk------sgdYWHIEVEGitiGCNYAYRVYGk 94  Prochlorococcus...
NP_893439     10 LPLGSSITSGGVNFSLIATn-AEYIEILLFEKEDsvapktIFKLDHshk------tgsYWHAEINNlkeGSIYAFRVKQn 82  Prochlorococcus...
YP_856910      9 RQLGATPEHDGVNFAIWARl-ASRVELLLFASADdtt-peVIPLSPrlnr-----tayYWHIHIEGielGQRYAYRIQGp 81  Aeromonas hydro...
YP_942334     10 HPIGATVEDGGVNFCLYCDerSTSVELLLFEQHNalepyeIIRFDPlihr-----sfyFWHIFVKGlpeKTHYAYRVGGp 84  Psychromonas in...
YP_001015569  10 WPLGSSVTLDGVNFSIAAPh-ATNLELLIFQSADdeyakeTISLDQknr------sgdYWHVEIEGlaeGTLYGYKVSId 82  Prochlorococcus...
YP_002892248  12 RQLGATPEGHGTNFAIWGRl-AKSMELLLFSSEKde--hpEVILLKeqey----hsgyYWHVHVSGvnaGQLYAWRVKEa 84  Tolumonas auens...
ZP_06307131   18 QPLGATVTPQGVNFSLFSEa-ATGVELLLFDQHDd-----IHPFQIipfdpiinktfhFWHVLVLGlptGTHYAYRVNGp 91  Cylindrospermop...
YP_003899918  25 QPLGAIVDANGVNFSLYSAh-ATAVELLIFEKYDd-----LYPVQIikldpatnktfyYWHVYVEGlkpGAAYGYRVDGp 98  Cyanothece sp. ...
ZP_08079285   19 KTLGATIEKDGVNFAVWCPa-ASIIELLLFKDIDdce-pdIITLASplfr-----styYWHVKVKGikaGQIYAWRIKEa 91  Succinatimonas ...
2WSK_A        78 wqpae--ghrfnpAKLLIDPCARQIDGefkdnpllhaghn------------------epdyrdnaaiapKCVVVV 133 Escherichia coli K12
NP_875794     95 gdlgd---ankhcGKILLDPCAREISGwdefqrissk-----------------------eeipnlsnclKGIVSE 144 Prochlorococcus mar...
NP_893439     83 dngl----nknysKTVLIDPCSRGITGwsnykrknal-----------------------nnidnmdcclKSVVCD 131 Prochlorococcus mar...
YP_856910     82 wrpyy--gtrfdaDKVLLDPYGRSIELganydrwaaarpgsnlaecaknrvvdtrhydwegdklpahslsRSVIYE 155 Aeromonas hydrophil...
YP_942334     85 fqpen--glvfdpQKVLIDPYSKGINCslwqrndackagdnlhtsmrs-cvlkltdydwqgdsppnhhlnESIIYE 157 Psychromonas ingrah...
YP_001015569  83 gqk-------aaeDHIVLDPCARAITGwenyirnhke-----------------------kvnrgyahclKGVVTK 128 Prochlorococcus mar...
YP_002892248  85 lsnrp--gthfdpEKALLDPYGRRIVLsenydrqlsa-----------------------ksgsnlhccaKNVVTD 135 Tolumonas auensis D...
ZP_06307131   92 drpqd--ghrfdaKKVLIDPYSKGNNKtlwnrgkact------------------------pgsnlessiRCVVID 141 Cylindrospermopsis ...
YP_003899918  99 nnlhea-ghrynkNKVLLDPYSKSNSCilwkrinalg------------------------tednlttsmRSIVVD 149 Cyanothece sp. PCC ...
ZP_08079285   92 lrtykfaqshvelGKVLLDPYGKRVLFpkayrrfqtd-----------------------dpqenlkisaKSAVVD 144 Succinatimonas hipp...
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