Conserved Protein Domain Family
GltS_FMN

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cd02808: GltS_FMN 
Click on image for an interactive view with Cn3D
Glutamate synthase (GltS) FMN-binding domain. GltS is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of L-glutamate from 2-oxoglutarate and L-glutamine via intramolecular channelling of ammonia, a reaction in the plant, yeast and bacterial pathway for ammonia assimilation. It is a multifunctional enzyme that functions through three distinct active centers, carrying out L-glutamine hydrolysis, conversion of 2-oxoglutarate into L-glutamate, and electron uptake from an electron donor.
Statistics
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PSSM-Id: 239202
View PSSM: cd02808
Aligned: 98 rows
Threshold Bit Score: 213.557
Threshold Setting Gi: 83590027
Created: 20-May-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1EAO; Azospirillum brasilense Glutamate synthase binds FMN, a 3Fe-4S cluster, and 2-oxoglutarate
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1OFE_A       798 SPEMSKSLHKAvaaykvggngnngeayDHYELYRQYlkdr-----------pvtaLRDLLDFNadqpaislee------- 859
1EA0_A       791 EGGVIHTLQQAvtn----------dsyTTFKKYSEQvnkr-----------ppmqLRDLLELRstkapvpvde------- 842
gi 15643816   22 SFRGREVLYPGpfgdita--gavkdypVDYSHLNILgyargaeg----lpegvepGPDTAIFTnvdttteyg-------- 87
gi 42523699   79 YILESIRPEINqyfiesntdgkpfnreQRSVVYQRAkkvldt--------vpfgtQHNVYAQGyefvthsmypkhvdrkd 150
gi 82499230   48 SFRGREVLYPGpfgevta--ganknypVDYSHLNIQgyavgakg----lpegveaNPDTAIFSnvdttteyg-------- 113
gi 83590027   42 SMVGRKLLYPRdfgtvta---gssnttHVGISYNSLrilgynygaygltkglsnsADDCIFPNvnvetefgs-------- 110
gi 86135569   65 WVFEEEREKIRqyfiendlngtplsreQRSLVYQRSkkqtet--------ipfgtQHNLYNDGyefvkhslfpkdhhdvt 136
gi 88804116   63 NLLEKQRHVFQeivllnraegkpfnwiQKEIIYKRAadanks--------qpfgtQIPYDKVGrewfthstypvkevndd 134
gi 91201651   46 AFRSVENLYPQpfgvita--gadkeypVDFSHLNIMgtavga--------vgiapDSDKAIFDnvntetrlg-------- 107
gi 91202507   47 AYRGPEAIYPVpfgeita--gaqkdfpVDFSHFNITgsit------------ghhQKEYDLFTnacietkmg-------- 104
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                              ####                          #                           
1OFE_A       860 ---------vesvesivkRFCTGGMSLGALSREAHETLAIAMNrlGAKSNSGEGGEDvvryltlddvdsegnsp-----t 925
1EA0_A       843 ---------vesitairkRFITPGMSMGALSPEAHGTLNVAMNriGAKSDSGEGGEDparfrpdk--------------- 898
gi 15643816   88 ---------wdikvkmkvPIFTGALGSTEIARKNWDHIAVGAAisGITVVCGENVAGvdpdleldsngkvkkspeldrri 158
gi 42523699  151 -lrvtigtdlckqpysmsLLNISAMSFGSLSTASILALNGGAKdgGFAHNTGEGGISpyhle------------------ 211
gi 82499230  114 ---------wekkvkmkvPIFTGALGSTEIARKNWEHFAIGAAisGITLVCGENVCGvdpeleltsdgkvkkspem-drr 183
gi 83590027  111 ----------evktkvkaPIMTGALGSTFIAAKYWDSFSIGAAlvGIPIVIGENVVGidkeaviengrivkapel--drr 178
gi 86135569  137 gdrivygsdkcsqkletsILGISAMSFGSLSKNAILALNQGAKmgNFTHNTGEGGVSpyhl------------------- 197
gi 88804116  135 -frvtvgsslctqpysssILNLSGMSYGSISKNATLALNGGAKiaGFAQNTGEGGFTpyhqe------------------ 195
gi 91201651  108 ---------kdkgiklkmPIMIPGLGSTKVAKTHWDGLAIGSAisGTALTIGENVGGmdeqskisngkithcpdle-yrv 177
gi 91202507  105 ---------rdgglklriPIICTGLGSTKIAQTHFESLGSGAAicGTGIVLGENVAAmdddstitdgnvtnspelv-rrf 174
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          #                                      #     #                
1OFE_A       926 lphlhglqngdtansAIKQIASGRFGVTPEYLMs-----------------gKQLEIKMAQGAKpGEGGQLpgkkvsEYI 988
1EA0_A       899 --------ngdnwnsAIKQVASGRFGVTAEYLNq-----------------cRELEIKVAQGAKpGEGGQLpgfkvtEMI 953
gi 15643816  159 eiykryhdgeygeilIQMNVEDTRLGVAEYVINkh---------------giETIELKWGQGAK-SIGGEIkv--rsLER 220
gi 42523699  212 -----------pggdLIWQIGTGYFGCRTHDGDfdpelfkk----naahpqvKMIEIKLSQGAKpGHGGMLsgkkvtQEI 276
gi 82499230  184 ittykrfyegwgeilVQMNVEDTRLGVAEYVIEkh---------------glDTIELKWGQGAK-CIGGEIkv--ksLER 245
gi 83590027  179 iqtylkyydgfgaiiVQMNVEDTRNGVAEYVIEkyg--------------dqVILELKWGQGAK-DIGGEIqv--tdLEY 241
gi 86135569  198 -----------qggdLVFQVGTGYFGAGKTNDEgkrvfdagifkenalkpevKMIEIKLSQGAKpGHGGILpakkntKEI 266
gi 88804116  196 -----------ygadIIFQIGTGYFGCRTKEGNfdtekfrd----ialhevvKMIEIKISQGAKpGFGAILpakkntEEI 260
gi 91201651  178 ktyqswqqdgyglivMQENVEDSRLGILEYGVEkl---------------gvQAVEMKWGQGAK-DIGGEVki--nnLEK 239
gi 91202507  175 ksfkdnqkdgygfiaIQANQEDWRLGVLPYSLEkl---------------gaDAVELKWGQGAK-NIGGEVkl--tsIEK 236
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #                                                                    #       
1OFE_A       989 AMLRrskpGVTLISPPPHHDIYSi---------------------------eDLAQLIYDLHQINpeaQVSVKLVaeigi 1041
1EA0_A       954 ARLRhstpGVMLISPPPHHDIYSi---------------------------eDLAQLIYDLKQINpdaKVTVKLVsrsgi 1006
gi 15643816  221 ALELkr-rGYIVLPDPELPEVQRafkegeikefe-------rhsrlgfvskeSFLKEVERLRKLGf-kRITLKTGay-sa 290
gi 42523699  277 ANIRnvpmGKDVISPPGHKAFSDs---------------------------rGMLAFITKLRELSggkPIGIKLClg-hr 328
gi 82499230  246 ALELkk-rGYVVLPDPTLKEVQEafkkgalrefe-------rhsrlgfvekeSFLKEIERLRSLGf-kRITLKTGay-sa 315
gi 83590027  242 AIFLkn-rGYVVDPDPTIPEVQEafksgairsfarhsrlgytnltsfeqvreNFMTAIEYLRGLGy-kRITLKTGsy-gm 318
gi 86135569  267 AEIRvveeGTDVLSPPSHSAYSNf---------------------------eEMVAFIQEVRDLSggkPVGIKLCvg-nn 318
gi 88804116  261 AKFRvvekGKQILSPPHHSAFGSd---------------------------eEMIFFIDKLRKLSngkPIGIKLCig-qk 312
gi 91201651  240 AKMLrd-rGYIVLPDPYDKEVTGsfgksfkefer--------hsrvgmvnedDFVKRVKALRNAGa-kYVFLKTGay-rp 308
gi 91202507  237 AIRLkk-lGYVVIPDPEDALTQQafkngtikgferht-ricaipdamsrafdGFMNNVQHLRDAGa-kYVFLKTGsy-rp 312
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                   ###                                        # #       
1OFE_A      1042 GTIAAGVAKAn-----ADIIQISGhdGGTGASPLSSIKHAGSPWELGVTEVHRVLMENqlr--drVLLRADGGLKtgWDV 1114
1EA0_A      1007 GTIAAGVAKAn-----ADIILISGnsGGTGASPQTSIKFAGLPWEMGLSEVHQVLTLNrlr--hrVRLRTDGGLKtgRDI 1079
gi 15643816  291 VELAMALRYGae--akVDLITVDGapGGTGMSPWPMMNEWGIPTFYLEALTYQFAEKLsrrgirvPDIAIAGGFSteDGV 368
gi 42523699  329 NEFEELVSLMsvekiyPDFIVVDGaeGGTGAAPLEFTNYIGMPGMDALVIVVDTLKKAglk--dkIKVIATGKITtaFDI 406
gi 82499230  316 VELAMALRFGae--akLDLITIDGapGGTGMSPWPMMNEWGIPTFYLEALAYQFAEKLtkrgfrvPDLAIAGGFSteDGV 393
gi 83590027  319 EALAMAIKLAsd--akLDLLTVDGsgGGTGMSPWNMMETWGVPSILLHSKAYEYASLLaargkkvVDMAFAGGLAreDHI 396
gi 86135569  319 EEIEEMIKTFstlnnyPDYIAVDGgeGGTGAAPLEFTNYMGTPLIEGLLFIHKVLENNnlr--kqIKIIASGKAMnaFDV 396
gi 88804116  313 DEFDSMAQSLaknqnyPDFIAIDGaeGGTGAAHMESLHWAGLPIVEAIHFAHSTLVKHkir--deIKLMAAGKIIsaFDI 390
gi 91201651  309 ADLARAVWYCsi--agVDVLTVDGagGGTGMSPWHMMNEWGVPTLYISALTYNYVHQLaskghyvPDIILAGGFAfeDDI 386
gi 91202507  313 SDLARAIKFAsw--arIDVLTIDGsgGGTGMSPWRMMNEWGLPTVYIAALTYEYCKRLadrgeyvPDIVLAGGLVseDHI 390
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     ##       #                         #    #                          
1OFE_A      1115 VMAALMGAEEYGFGSIAMIAEGCima--------------------rvCHTNNCPVGvatqqerl-------------rq 1161
1EA0_A      1080 VIAAMLGAEEFGIGTASLIAMGCimv--------------------rqCHSNTCPVGvcvqddkl-------------rq 1126
gi 15643816  369 LKAIAMGSPYVKAVCMGRALMIPamvgknig----------ewlksgnLPKTVSKYGttveeifvtyeelrsrfgeeevk 438
gi 42523699  407 IKLLCLGADATYAARSMLLALGCiqa--------------------lrCNNNKCPTGvatqdpnlv-----------rgl 455
gi 82499230  394 FKAIAMGAPYVKAVCMGRALMIPgmvgknie----------kwlkegnLPKTVSKYGttpeeifityeelk-ekygdeik 462
gi 83590027  397 FKALALGAPYVKLVCMGRALMIPgfvgsniegvlhperrakvngnwdsLPRTVAELGtkaeeifagyydvqkkvgaeemk 476
gi 86135569  397 VRLLALGADAVGMARSFMLSLGCiqa--------------------reCNMDTCPVGvatqdedln-----------eal 445
gi 88804116  391 YRMLALGADACYSARGMMFALGCvqs--------------------lkCNLDKCPTGittmepsrv-----------ksl 439
gi 91201651  387 FKAFALGAPYVKAVGMARSPLCAshvgtvva----------nqikegkIDKFIADYGsnveeifvlaskvk-rlfgtgkk 455
gi 91202507  391 YKSFALLSPFIKAVGMSRSTICAamvgntig----------kavaegkVPKPVSEYGsstdqifyysqk-----vkdrfg 455
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|....
Feature 1                                                    
1OFE_A      1162 rfkgvpGQVVNFFYFIAEEVRSLLAHlgyrslddiiGRTDLLKV 1205
1EA0_A      1127 kfvgtpEKVVNLFTFLAEEVREILAGlgfrslneviGRTDLLHQ 1170
gi 15643816  439 klplgaIGVYTFVQKFKTGLQQLMAGarkfr-lsalSRKDLIAL 481
gi 42523699  456 hiptkrVRVKNFHQETLESVAHVIGAmgvar-hqdlNRSHLYKR 498
gi 82499230  463 niplgaIGIYTFVQKFKTGLQQLMAGsrnfr-istiSRKDLIAL 505
gi 83590027  477 nipygaIAFWTLADKLTAGLQQLMAGarkfs-ldqiTRNDIASA 519
gi 86135569  446 vvakknIRVKNYHNETIKAIKEVAGAmgidn-lgnlNPTHIFRR 488
gi 88804116  440 vvedkkAKVANYHKNTMEAFKELLGSmglen-rkgiQKKYIVRR 482
gi 91201651  456 evppnaLGLYSYYQRLSQGLRQLMCGsrkfa-leyiTRDDIVTL 498
gi 91202507  456 niptgaIGMYSYYQRLATGLRQLMAGsrrws-vndiTRDDIFTL 498

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