Conserved Protein Domain Family
DUS_like_FMN

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cd02801: DUS_like_FMN 
Click on image for an interactive view with Cn3D
Dihydrouridine synthase-like (DUS-like) FMN-binding domain. Members of this family catalyze the reduction of the 5,6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes, and also in some archaea. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown, but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities, which may overlap. 1VHN, a putative flavin oxidoreductase, has high sequence similarity to DUS. The enzymatic mechanism of 1VHN is not known at the present.
Statistics
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PSSM-Id: 239200
View PSSM: cd02801
Aligned: 194 rows
Threshold Bit Score: 113.744
Threshold Setting Gi: 71081287
Created: 20-May-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1VHN_A; Thermotoga maritima TM0096 binds FMN and a sulfate ion; contacts at 3.5 A
    View structure with Cn3D
  • Comment:the sulfate ion is thought to be coincident with the natural ligand binding site

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                          #            
1VHN_A        6 KVGLAPXAgyTDSAFRTLAFEwg-----aDFAFSEXvsakgflxnsqk----teellpqphernVAVQIFgsepNELSEA 76
gi 2688667   17 IMILAPMEdvTDTVFRNLIHLigsaegepHIYFTEFistkgilngskqsv-qhvflkpnelnrpLIAQIWgnvpEQFYRA 95
gi 18144936   2 KYYLAPMEgiTGFIYRNSYEKffg---giDKYFAPFvvpnsskslktke--lrdvlpennkginLVPQILtndsEGFILT 76
gi 28270177  21 FFTMAPMEavSNTVFRQVISHaa----apDTFFSEFvyaksitnpntkfpvhgrlyvaaaesqkPVVQLWgneaADFASA 96
gi 29340641   6 PIHFAPLQgyTEAIYRNAHDAffg---gvDTYYTPFvrlekgnfrrrdv---rgiepenngvphLIPQLIassaEKAEVI 79
gi 39930914  13 FFVLAPMEdvTDVVFRHVVSEag----rpDVFFTEFtnsesychpdgkds-vrgrltftedeqpIVAHIWgdkpENFRKM 87
gi 50877800   6 RLSLAPIRglTTASFRSLFCKhfa---gfNSSIAPFinpqkrasfdnkm--lkdvlpeanmvvpVIPQLLhnnaEDFIAL 80
gi 67940782   7 VIYLAPFQgiTTLTYREVYVRhfr---gvDKLYTPFytqiprsqavask--ikeleitaqnriqLVPQILskdaDEMLRF 81
gi 78192736  24 PLMLAPMQglTNRALRALFINwv----rpDVVFTEFmrvnpggarqpiragdlrevaaveggvpLVVQLIghdhKALVAA 99
gi 88801417   4 TLLSSPLQgfTDYKFRNAFNHffg---giDTFYSPYirlngkmviknsy--qkdlllennseleVIPQIItndaEEFLFV 78
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        ##                                        # #                  
1VHN_A       77 ARIlse-kyKWIDLNAGCPVRKVVKEGAGGALlkdlRHFRYIVRELRKSVs---gKFSVKTRLGwekn---eveEIYRIL 149
gi 2688667   96 IEIlgsmgfWGIDINMGCPKSKIIKKGVCSALinnkSLAKEIILASKEACarfnlPLSVKTRHGfsypevddwlGFLLGL 175
gi 18144936  77 AKKlkdlgyNEINLNLGCPSGTVVGKKRGSGFlahkEELDKFLEEIFKIDd---iKISIKTRLGmdkp--eefyELIKIY 151
gi 28270177  97 TAElrdrgfEAVDINMGCPDGTVIKNHGGSDLirnpQWAEDVIAAAKTSGl----AVSAKTRLGyskv--aeyhDWIATL 170
gi 29340641  80 LSLfiekgyQEVDINLGCPFPLLAKRHNGSGIlpypEEVKALLSIVTRYPq---iSFSVKMRLGweqp--declALAPIL 154
gi 39930914  88 SIGmaelgfKGLDINMGCPVPNVAGNGKGSGLicrpAVAAELIQAAKAGGl----PVSVKTRLGytdv--dewrEWLTHI 161
gi 50877800  81 AGRlaelgyKEINWNLGCPAPMVAKKKRGSGMlahpDLIVEFLEEVLPQIp---vKLSIKTRLGysnt--aqtkELLPRL 155
gi 67940782  82 AMHchekghTEINWNMGCPFPRVANKQRGSGMlphpDLVESILQKITPQLp---lNLSVKCRLGyeda--seidALIPIF 156
gi 78192736 100 ARTaqeagaVHINLNLGCPYGRMTSGLTGGGMlhrpEALEELIPALRRAIa---gTFSVKVRAGydds--gqimTLLPLF 174
gi 88801417  79 SKYvralgyKELNWNLGCPYPMVTKRGMGSGLisdaEKINSILHKVHNESd---iLVSMKMRMGyenp--eeilGVLPIL 153
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ## ##                                 ##                       #       
1VHN_A      150 VeegvDEVFIHTRTvvqsftGRAEWKALSvle-------krIPTFVSGDIftpeDAKRALees-gcDGLLVARGAIGRPW 221
gi 2688667  176 Ei---DMLTVYPRLavnqskGPVNFDIFYelvklrnnlspsTLIIGNGDVlslkEARYYVdky-liDGIMFGRGIFDNLN 251
gi 18144936 152 NkypmEELIIHPRTrqdfygNKPNLEVFKdais-----lskNPVCYNGDIftleDHNKLVeefkevDKIMLGRGVLANPA 226
gi 28270177 171 LsqhvAVLTVHLRTkqemskVPAHFEVIDdlikmrdaiapeTLLQINGDVadyqAGVALAkahpglDGIMIGRGVFANPF 250
gi 29340641 155 NdlplRQITMHPRLgkqgykGEVDLQGFSafre-----vcqLPLVYNGDIhnleDIQRIStqfpslAGIMIGRGLLANPA 229
gi 39930914 162 LkqdiANLSIHLRTraemskVDAHWELIPeikklrdevapdTLLTINGDIpdrqTGLKLAeqy-gvDGIMIGRGIFTNPF 240
gi 50877800 156 DqfplTEIIIHARLgkqmykGTTDPDTFAeclq-----lsqHRIAYNGDIttleHFHELQtrfpqvEHWMIGRGALADPF 230
gi 67940782 157 NehriSELTVHARLgkqmysGEVNWSAIQklln-----nlkIPFAYNGDIfsveDYQKFKitfpqiNTLMIGRGLLTDPF 231
gi 78192736 175 EeagvDFLVLHPRTvvqeytGHADHAVTAevvr-----qtrLPVIANGDVrsaaDGLRVLrkt-gaTGLMLGRGAIADPL 248
gi 88801417 154 DtyplKNIAIHARIgkqlykGGTDLEAFQkcld-----nskHKMYYNGDItsvvAFHKLQerfpsiDHWMIGRGLIGDPF 228
                       250
                ....*....|
Feature 1                 
1VHN_A      222 IFKQIKDFLR 231
gi 2688667  252 LFKPFSKHFL 261
gi 18144936 227 LMNEILNNEF 236
gi 28270177 251 AFESQPQSHD 260
gi 29340641 230 LALEYKENRT 239
gi 39930914 241 AFEKEPKEHS 250
gi 50877800 231 LAERIKGIGK 240
gi 67940782 232 LPAKLKGLEL 241
gi 78192736 249 LFQRLRGKAP 258
gi 88801417 229 LPSMIKADTT 238

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