Conserved Protein Domain Family
NRK1

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cd02024: NRK1 
Nicotinamide riboside kinase (NRK) is an enzyme involved in the metabolism of nicotinamide adenine dinucleotide (NAD+). This enzyme catalyzes the phosphorylation of nicotinamide riboside (NR) to form nicotinamide mononucleotide (NMN). It defines the NR salvage pathway of NAD+ biosynthesis in addition to the pathways through nicotinic acid mononucleotide (NaMN). This enzyme can also phosphorylate the anticancer drug tiazofurin, which is an analog of nicotinamide riboside.
Statistics
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PSSM-Id: 238982
View PSSM: cd02024
Aligned: 38 rows
Threshold Bit Score: 163.65
Threshold Setting Gi: 39580980
Created: 20-Oct-2004
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Active site
Feature 1:Active site [active site]
Evidence:
  • Comment:GxxxxGK[S/T], signature p-loop motif, also known as Walker A motif, present in various triphosphate nucleotide binding proteins. The Lys residue within the motif plays a major role in the phosphoryl transfer.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #    ####                                                                  
gi 6324200     8 LVALSGCSSSGKTTIAKLTasl------------------ftkATLIHEDDFYkHDNEVPvdak---yniqNWDSPEAld 66
gi 32414919   10 VIGISGCSSSGKTTLARLLrdi------------------fpnTFILHEDDFYkPESEIPmke-----gltNWDCPEAls 66
gi 39580980    8 IFALAGCTNSGKSTLAKEFqrhf----------------gaaeTTIINQDEFYkTEDEVDki--------yHPAAVTLte 63
gi 17509279    8 VLALAGCTNSGKSTLAKEFqrff----------------gageTTIINQDEFYkTENEVEki--------yHPDAEHLtd 63
gi 19112395    7 IVGVSGASCSGKSTLCQLLhai------------------fegSSLVHEDDFYkTDAEIPvkn-----giaDWDCQESln 63
gi 40745970 1168 IFALSGPSSSGKTTLARLLqrifsnqpqtq---egsqlsklisTFIIHEDDFYySDDKIPytttksgariqDWDCLGAld 1244
gi 42549945    8 IVGISGCSSSGKTTLARLLrdi------------------fphTFILHEDDFYrPENELPtkn-----gllDWDCAEAid 64
gi 46101999   55 VVGVGGATCSGKTTLAKHLiqilnpladaagcssgshrggitdAFILHQDDFApPEATLPvnee---fgvtDWDHPPTa- 130
gi 38102622    8 LVGLSGCSSSGKTTLARLIrdi------------------fpnTFILHEDDFYkNESELPkra-----ghaDWDCVGSln 64
gi 50256699   12 IGIGRGASCSGKTLLAKHIrral-----------------ptdAAIIHQDDLCpPAEEVPyskqy--pdlqDWDDPETci 72
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 6324200    67 f-klFGKELDVIKqtgk--iatkLIHNNnvddpftkfhidrqvw------------------------------------ 107
gi 32414919   67 i-pdMTAALEHIRatg----elpVSSTMnltrpflsrrhthgssstttssssyyppssssaaaskaappkqktgpgktit 141
gi 39580980   64 --rgYYWSFDEKEainidqfkerILQESkl-------------------------------------------------- 91
gi 17509279   64 --egYYWSFDEKEainidqfrqrILNESkv-------------------------------------------------- 91
gi 19112395   64 l-daFLENLHYIRdhgv--lpthLRNREnknvapealieyadi------------------------------------- 103
gi 40745970 1245 i-kfLTSALRYVHehgh--lpprLRSKEdlnevspgsgvddalvdelrgyvss--------------------------- 1294
gi 42549945   65 i-paMAESLAYIRqhaa--fpptLDSKEdqnsvgkcpvpdstinaqr--------------------------------- 108
gi 46101999  131 ---iQDAQIDYKRmygt---ikhIKSTGsfppeftshdhlnsqpdcpisetv---------------------------- 176
gi 38102622   65 i-eqMEKSLSYIRengq--fppdLNSIEdqnavgkcpvsdakiqelktk------------------------------- 110
gi 50256699   73 lwseFRALLRQVRetgh--vgdhDTHDHlnkenkievneqiferwq---------------------------------- 116
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 6324200   108 ------------------------------------------------------delkakydsinddkyeVVIVDGFMIF 133
gi 32414919  142 takskslsnspkqptldskedlnsvgpcpittdfinkikarvadwlqpsspghrvlhlsshqqqqqqplkICIIDGFLLY 221
gi 39580980   92 --------------------------------------------------------------------fkNVIIDGNMIT 103
gi 17509279   92 --------------------------------------------------------------------ykNVIIDGNMIT 103
gi 19112395  104 -------------------------------------------------------ikefkapaiptleqhLVFVDGFMMY 128
gi 40745970 1295 ---------------------------------------------rtqmillssssmvaeerldgkekvtIALLEGFLLY 1329
gi 42549945  109 ----------------------------------------------------akvdaalgpdhplrnnlrLCLLDGFLLY 136
gi 46101999  177 -----------------------------------------------sktwkdkfgsltslntfsagdvrILLADGFLLY 209
gi 38102622  111 -------------------------------------------------vaewskpdgpghgllvpgdlgLCLLDGFLLY 141
gi 50256699  117 ----------------------------------------------------rrfeeltkqqreqgveliWVIVDGFVLY 144
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 6324200   134 NNtgis------------kkfDLKILVRApyevLKKRRASRKGYqtld-------------------------------- 169
gi 32414919  222 SPppllpa-------tllsqmDIKLFLLVskakALQRREARDGYvtle-------------------------------- 262
gi 39580980  104 EMdeiv------------elcDQIVVLTLdfktCRRRREARTDYv----------------------------------- 136
gi 17509279  104 EMdeiv------------dlcDRIVVMTLdfktCKRRREARTDYv----------------------------------- 136
gi 19112395  129 VNedli------------nafDIRLMLVTdfdtLKRRREARTGYitle-------------------------------- 164
gi 40745970 1330 APpatekggkehglrgvhdqiDLPMFLAApydlVKERREKRSGYvtigpapttelpqrsssaseaeeervdleveddrpp 1409
gi 42549945  137 SPsmaav----------kpnlDIKLFLRTtyakAKARREARDGYvtle-------------------------------- 174
gi 46101999  210 YDpdvr------------stmDVKVFLRTpraiLRQRREERFGYatae-------------------------------- 245
gi 38102622  142 TPplkhi----------msllDLKFFLLVsratAIRRREARIGYvtve-------------------------------- 179
gi 50256699  145 YDkdvv------------dmlDIRIFLRVphdvLKDRREERQVYvlqr-------------------------------- 180
                        330       340       350
                 ....*....|....*....|....*....|
Feature 1                                      
gi 6324200   170 -sfwVDPPYYFDEFVYESYRANHAQLFVNG 198
gi 32414919  263 -gfwKDPPGYVEKIVWPEYVRAHEWMFEGG 291
gi 39580980  137 ---pPDTPGYFENVAFPAYLRHLENARKRS 163
gi 17509279  137 ---pADTPGYFVNVAFPAYVRHLERARQRS 163
gi 19112395  165 -gfwQDPPHYFENYVWPGYVHGHSHLFVNG 193
gi 40745970 1410 qnfwTDPPGYVDDIVWPRYVRDHAWLLLPE 1439
gi 42549945  175 -gfwADPPGYVDKIVWPNYVEEHAWMFEDG 203
gi 46101999  246 gtvwKDPEGYFDNIVWPAYELAHKGVFSNH 275
gi 38102622  180 -gfwADPEGYATDVVWPNYAESHAWLFKDG 208
gi 50256699  181 -gvwIDPPEYFNKIVWPGYLKAHAHVFEDP 209

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