1KO7,1KKM,1KNX


Conserved Protein Domain Family
HprK_C

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cd01918: HprK_C 
Click on image for an interactive view with Cn3D
HprK/P, the bifunctional histidine-containing protein kinase/phosphatase, controls the phosphorylation state of the phosphocarrier protein HPr and regulates the utilization of carbon sources by gram-positive bacteria. It catalyzes both the ATP-dependent phosphorylation of Ser-46 of HPr and its dephosphorylation by phosphorolysis. The latter reaction uses inorganic phosphate as substrate and produces pyrophosphate. Phosphoenolpyruvate carboxykinase (PEPCK) and the C-terminal catalytic domain of HprK/P are structurally similar with conserved active site residues suggesting these two phosphotransferases have related functions. The HprK/P N-terminal domain is structurally similar to the N-terminal domains of the MurE and MurF amino acid ligases.
Statistics
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PSSM-Id: 238899
View PSSM: cd01918
Aligned: 39 rows
Threshold Bit Score: 84.5939
Threshold Setting Gi: 46191878
Created: 10-Jan-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1KO7_A; Staphylococcus xylosus HprK
    View structure with Cn3D
  • Comment:phosphate ions are stabilized by interactions with residues at the subunit interface

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                 # ###                      ##                            
1KO7_A      131 RTTSLHGVLVDvygvGVLITGDSGIGKSETALELIKRg-------HRLVADDNVEIREIsk--------dELIGRAPKLi 195
gi 15963795   4 PFVNVHGTAIVlgttGLLITGPSGSGKSALALSCLSEvrhr-grfAALVADDRVDLTLEn---------gRIVARCPAAi 73
gi 23502940   6 ERSGLHATTLQllgrGVMIMGRSGAGKTELALTLIERaasr-reeAFLVADDRTLLHREg---------dRLIASVPTAl 75
gi 16124494   7 LIALRHQGLWR----GALIEGPSGAGKSDLALRALDQg-------FRLVADDRVVTFAAg---------gRLYGRAPETl 66
gi 48763952   4 PPPTVHGTAIArdgwAVLLRGPSGSGKSDLALRMIDRg-------AVLVGDDQLIVLAGp---------aGPALAPPDPl 67
gi 27356431   4 GGPSVHASAVKvgnlAVLIRGPSGSGKSRLAFDLIMAgragvverAVLVGDDRVHLATVg---------dEIEVRPAPPl 74
gi 49474884   4 KNKILHANCLQlggkGLLIIGPSGSGKSTLTLSLLDRagws-kreAKLISDDYTMLRVEn---------gKLQGSTPEGl 73
gi 45916660   1 MAANHHGTLVLtgdrGVFITGAPGMGKTTLALALLRHcaac-grlGRFVSDDQVFISGAg---------gRLVGRAAPAi 70
gi 39933433   5 APRTVHASAVKvgdlAVVIRGPSGSGKSRLAWALILAgragqiprAELIGDDRVFMEPEqg--------qLTVRQVPELa 76
gi 48765369 111 GVLPLHACAIRtgkgGLAIVGNSGAGKSTTATALVQRg-------HRLLGDDVLVVDPDtglaqpcfpmvKLWDSSRSAl 183
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               # #                                                                     
1KO7_A      196 ----eHLLEIRg-LGIINVMTLFga--gSILTEKRLR--LNIHLenwhkeklydRVGLNEETLril---dteITKKTIPV 263
gi 15963795  74 ----rGLIEIRg-SGIAEVDTVSa----CVLDWAIMPv-RAPFDp---------RLPPEEELQlei---grnLPLLRLPV 131
gi 23502940  76 ----aGGVEIRg-AGLFRIPYRV-----ATPLDLVVL--LVNGDea-------eRYPGKERWIfee----vsLPRLLLPA 132
gi 16124494  67 ----aGLIEVRg-LGVVTAAAVSfa-eiALVIRCVDTpgRVERLpe-------pRFERIAGVD---------VPVFDLWP 124
gi 48763952  68 ----rGLIEVRg-LGLVEMPAAId----PVPLALVVD--LVPGGpi-------eRLPDPAQADigg----asVALVRLDP 125
gi 27356431  75 ----aGLIEIRg-LGIRRCDFVE-----HATVGLVVD--LAAADa--------eRLPPAESLKtsi----lgVEIPRIPV 130
gi 49474884  74 ----qGGIEIRg-VGLYTIEFEK-----QTTIDCVIL--LGPEYe---------RFSTNQTFKfad----lhIPLLKLPS 128
gi 45916660  71 ----hGLAEARg-FGPAPVSYEM-----AAVIDLVIE--LVPADq--------aPRYQEAGTVrle---gvdLPTLRLAE 127
gi 39933433  77 -----GLIEIRg-LGLRQTASVA-----AATVGLVVD--LGAADa---------SRMPETAALrte---iggVQLPRVPV 131
gi 48765369 184 digqeGQQSVGgkIGKWHMPMLAafdpvPRPLSAIVH--LRADPa--------aRRPVLTRWPrphaaavldAMTYRVYL 253
                       170       180       190
                ....*....|....*....|....*....|..
Feature 1                                       
1KO7_A      264 rpg---------------rnVAVIIEVAAMNY 280
gi 15963795 132 e------------------gPLSPVDALAALL 145
gi 23502940 133 lsang-----------dsnaLSRAIEAFLFFE 153
gi 16124494 125 p------------------ePAAPAKIRRMMQ 138
gi 48763952 126 feas------------apvkLDLALAVARGDS 145
gi 27356431 131 sr----------------dySPLPLVVAALTT 146
gi 49474884 129 lhe--------------adcTAICQAIEAFFF 146
gi 45916660 128 c------------------nADSAIWAIAAQL 141
gi 39933433 132 aa----------------gyDPLPLVAAALTT 147
gi 48765369 254 adalhgrrhvlsaatrlaahVPVWELVRTADF 285

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