1X3S


Conserved Protein Domain Family
Rab18

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cd01863: Rab18 
Click on image for an interactive view with Cn3D
Rab GTPase family 18 (Rab18)
Rab18 subfamily. Mammalian Rab18 is implicated in endocytic transport and is expressed most highly in polarized epithelial cells. However, trypanosomal Rab, TbRAB18, is upregulated in the BSF (Blood Stream Form) stage and localized predominantly to elements of the Golgi complex. In human and mouse cells, Rab18 has been identified in lipid droplets, organelles that store neutral lipids. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 206656
View PSSM: cd01863
Aligned: 16 rows
Threshold Bit Score: 255.699
Threshold Setting Gi: 7140576
Created: 6-Jan-2012
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II
  • Structure:1X3S: Human Rab18 binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #######         ##     #                              #                 
1X3S_A     16 LKILIIGESGVGKSSLLLRFTDDTFDpelAATIGVDFKVKTISv-----dGNKAKLAIWDTAGQERFRTLTPSYYRGAQG 90  human
AAP06068   12 FKLLLIGDSGVGKSSLLMRYTNDCFEsqlSATIGVDFKVKLVTvp----dGTKIKLSIWDTAGQERFRTLTPNVYRGSHA 87  Schistosoma japonicum
CAD27350   11 LKLLLVGDSSVGKSSILCRYSDNQFEekvLSTIGIDFKVKYLKi-----dNKTIKVGIWDTAGQERFRTLTSAYYRNAHA 85  malaria parasite P...
AAF37004    9 VKIVLLGESGVGKSSLLLSFSLGTFDgdvRSTIGIDFRTKDVSvvdsmgrQKKLKLHLWDTAGQERFRTLTSSYYRGAHA 88  Trypanosoma brucei
P36862     10 FKVLLVGDSGVGKSCILTRFTSGIFEestTSTIGVDFKVKYLTa-----dGKRCKLTIWDTAGQERFRTLTSSYYRGAQG 84  Volvox carteri
BAA21710    6 IKLLVIGESGVGKSSLIRRFVENKFDqnhDVTIGMDFKSKVMQv-----dGIDYKVALWDTAGAERFRSLTASFYRKALG 80  fruit fly
XP_818013   9 IKVFLLGDSGVGKSSLMLSFASGTFDpdiSSTIGIDFKVKNVEvmdadgvKRNVNIHLWDTAGQERFRTLTSSYYRGANA 88  Trypanosoma cruzi ...
XP_636019   7 YKVLLIGDSDVGKTSIVKRFSDDTFDedlLCTIGVEFKMKEVKv-----dGKKVDLCIWDTAGQEKFRALISSYYRGAHG 81  Dictyostelium disc...
AAM63924   14 FKVLLIGDSGVGKSSLLLSFTSNTFDd-lSPTIGVDFKVKYLTi-----gEKKLKLAIWDTAGQERFRTLTSSYYRGAQG 87  thale cress
AAP04086   14 FKILLIGDSGVGKSSLLVSFISSSVEd-lAPTIGVDFKIKQLTv-----gGKRLKLTIWDTAGQERFRTLTSSYYRGAQG 87  thale cress
Feature 1                                                ## ##                                
1X3S_A     91 VILVYDVTRRDTFVKLDN-WLNELETYct----rnDIVNXLVGNKIDKe-----------nREVDRNEGLKFARKHSXLF 154 human
AAP06068   88 AVFVYDVANRESFDNLKI-RMEELKTYsd----npNMIKLLVGNKIDSv-----------pRLISREEGLRFARLYNMPY 151 Schistosoma japonicum
CAD27350   86 IILVYDCTVRESFENLDV-WINEIDKYst----nkNAIKMLVANKIDKp-----------nHEVTKDEGKNFAFENNMLF 149 malaria parasite P...
AAF37004   89 VVLVYDVNEPQTFHALRK-WIDEADAFcrldgveeAVVYLLVGNKIDKcevgg----gatgMAVPKEDAQQLARDRHMLL 163 Trypanosoma brucei
P36862     85 IIFVYDVTRRDTFESLEAnWMREFDIYst----veSAIKMVVANKVDLsa----------qRQVSSEEGHDFARRHGCLF 150 Volvox carteri
BAA21710   81 AILVYDITSRDSLVKLET-WLAELDSYsd----npNIAIIVVGNKIDEe------------RVVDREEGRKFARKHRALF 143 fruit fly
XP_818013  89 VVLVYDVNEPQTFHGLQR-WMEEADVFcrnd-dedDAVYLLIGNKIDKcigggsegegvpfMSVPKEQAQQLAQRRRMLL 166 Trypanosoma cruzi ...
XP_636019  82 IILTYDVTKRESFDNLNY-WLNEVENFan----rsNLVKLLVGNKIDKe-----------nREVTREEGAEFAKKKAMLF 145 Dictyostelium disc...
AAM63924   88 IIMVYDVTRRDTFTNLSDiWAKEIDLYst----nqDCIKMLVGNKVDKes----------eRAVSKKEGIDFAREYGCLF 153 thale cress
AAP04086   88 IILVYDVTRRETFTNLVDvWGKEIELYst----nqECVRMLVGNKVDRes----------eRGVSREEGIALAKELNCMF 153 thale cress
Feature 1        ###                
1X3S_A    155 IEASAKTCDGVQCAFEELVEKI 176 human
AAP06068  152 AETSAKTSEGVNQLFSDIVTKI 173 Schistosoma japonicum
CAD27350  150 CETSAKNDINITYCFEELIQQI 171 malaria parasite P. falciparum
AAF37004  164 AFTSAKTRVGVEQAFDEVARSA 185 Trypanosoma brucei
P36862    151 VETSARANLAVGQAFEELLLKI 172 Volvox carteri
BAA21710  144 IETSAKCDQFVSDVFKDVVEKI 165 fruit fly
XP_818013 167 AFTSAKTRAGVEQAFDELARTV 188 Trypanosoma cruzi strain CL Brener
XP_636019 146 IECSAKSKIGIQQAFEELAQKI 167 Dictyostelium discoideum AX4
AAM63924  154 LECSAKTRVNVEQCFEELVLKI 175 thale cress
AAP04086  154 LECSARTRQNVEQCFEELALKI 175 thale cress

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