Conserved Protein Domain Family
PAPS_reductase

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cd01713: PAPS_reductase 
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This domain is found in phosphoadenosine phosphosulphate (PAPS) reductase enzymes or PAPS sulphotransferase. PAPS reductase is part of the adenine nucleotide alpha hydrolases superfamily also including N type ATP PPases and ATP sulphurylases. A highly modified version of the P loop, the fingerprint peptide of mononucleotide-binding proteins, is present in the active site of the protein, which appears to be a positively charged cleft containing a number of conserved arginine and lysine residues. Although PAPS reductase has no ATPase activity, it shows a striking similarity to the structure of the ATP pyrophosphatase (ATP PPase) domain of GMP synthetase, indicating that both enzyme families have evolved from a common ancestral nucleotide-binding fold. The enzyme uses thioredoxin as an electron donor for the reduction of PAPS to phospho-adenosine-phosphate (PAP) . It is also found in NodP nodulation protein P from Rhizobium meliloti which has ATP sulphurylase activity (sulphate adenylate transferase) .
Statistics
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PSSM-Id: 238846
View PSSM: cd01713
Aligned: 135 rows
Threshold Bit Score: 84.7638
Threshold Setting Gi: 54030425
Created: 12-Oct-2004
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Active Sites
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Active Sites [active site]
Evidence:
  • Structure:1sur, active sites according to reference
    View structure with Cn3D
  • Citation:PMID 9261082
  • Comment:Involved in substrate binding and enzymatic reaction

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1SUR         46 EYVLSSSFGIQAAVSLHLVNQIrp------diPVILTDTgyl-fpETYRFIDELTDKlklnlkvyratesaawq------ 112
gi 48697538   4 RIVIPISGGKDSQACLQLACAAhar----heiLGLFCDTnfe-hpKTYAHVEFMRKFygvkiqsitagsvle-------- 70
gi 5458143  235 NAIVPWSGGKDSTAALILARKVfd------dvTAVYVKMeye-mpLTDEYVEEVASKlninlvkvevpm----------- 296
gi 14601825 240 TVIVPWSGGKDSTAALLLAVEAfgr----davKAVYVDTgid-fiENAEYVEKVASTlgvdlvyaradvde--------- 305
gi 41615306 178 EIVIPFSGGKDSTTVAILAKKAkv------dfDLVFVDTgle-fkQTLEYIDYISKKikpinivyagidk---------- 240
gi 21243020  11 QHLVNVSGGKDSTATYLLGLESgr------dfRAVFADTgne-heLTYEFVRRLPERtggpaiewvkadfrarmakhkay 83
gi 50121553  20 INVISVSGGKDSLAQWLLAVKNgv------shIPAFADTghe-hpQTMEYLDYLESKlgkirrvhadfsrqiagkrkfia 92
gi 17484080   7 LHVVSLSGGKDSTATLCVALEQhgs----envRAVMADTgnedeqNLEYALDYLPRAlgipvdvvradftdefatkranl 82
gi 19886567 236 VLFVPVSGGKDSTCCLAIAVETtdrl--gidvEAVYVDTgyd-lgRDVVEDVGAALGvdikhvdvsdafe---------- 302
gi 54030425  48 VVAVGCSGGKDGTAAALQVAQYldrightgprVLVHADLgrvewkDSLPSCQRLADHlgwelmvvkraagdlmdrwqvrw 127
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                      #                
1SUR        113 -----------------------------------------earygklweqgvegieKYNDINKVEPMNRalke-----l 146
gi 48697538  71 -------------------------------------------kvwkyrrfpgggarHCTDELKIVPSRKfykelaenqg 107
gi 5458143  297 ---------------------------------------------pihkyglpthsnRWCTRMKVEALYNaikd-----i 326
gi 14601825 306 -------------------------------------------glliegmpmpdpeyRWCTGRKLEALRQafrtv---sr 339
gi 41615306 241 ---------------------------------------------kykmfgfdylkrRLCTKDKITTLYNyvrnn----y 271
gi 21243020  84 vlerwpqmgip------------dalvreaaelheptgnpfldlcitkgrfpsrmaqFCTSELKELPITEqvvlpm-lrr 150
gi 50121553  93 ekwpitlvsecgftd-----vqaeniiataldtlhptgipfldlcmwkgrfpstkrrFCSTELKHEPIRLqvvepivdag 167
gi 17484080  83 ariaagepesavygkrefmyrwtpeaaaralevlhptgipfldlclvrggfpsrkrqFCTQYLKTEQLVGyalrei-drg 161
gi 19886567 303 ---------------------------------------------rglrerkpthenRWCTGVKLSGIKKvlkdl----e 333
gi 54030425 128 r--------------------------------nnvaryadlscvklilpwstpamrFCTSELKGSVIMSalrkrf--pt 173
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       #                            #   #                              
1SUR        147 naqTWFAGLRREQSGSRanlpv-------------------laiqRGVFKVLPIIDWDNRTIYQYLQkhglkYHPLWDEG 207
gi 48697538 108 hgfEVWYGMRLGESTERakryrdridnelylphevlrkypkylgdMGIRFKLPLLEWDNVDVLEFLDg---kENPLYRDG 184
gi 5458143  327 enpVLIVGDRDSESAKRrlkppvv---------------eretsfGRILEVMPLKFWSGAMVQLYILasgikLHPLYYQG 391
gi 14601825 340 gktVVVTGDRDGESEKRgkrppl-----------------rydekLGYPVVSPLKLWSGGHVQLYILskgipLNPLYEAG 402
gi 41615306 272 enpVLLVGDRLIESEARlfrp--------------------rifkDEFLVAQPIKFFSYADEQILLEkenikLNPLYEKG 331
gi 21243020 151 gpvIQWLGIRAEESRHRarqpr------------------inrheSGCYLWRPIFDWKLQDVWKKHAdhgiaPNPLYALG 212
gi 50121553 168 fevVSWQGVRAEESASRallsewe---------------sgfdlgPRLSIYRPILHWKHADVFALAKrhgikPNPLYEQG 232
gi 17484080 162 yavWSWQGVRIDESDSRrerlqgtga----------cvkafevvgGGLFNYRPILRWSAADVFEAHAaagirPNPLYRQG 231
gi 19886567 334 gdvLLVVGDRDAESRRRrlrppv-----------------hrnrlLDVPEVNPVKGWGGAEVLGTLFrlglpVSELYEIG 396
gi 54030425 174 sdiCNVTGVRAQESSARkkmpvskv------------elklqrknTAGMSWNAIHEMEIEAVYQVIGd---aGLALHEGY 238

                ....*
Feature 1            
1SUR        208 YLSVG 212
gi 48697538 185 FDRVG 189
gi 5458143  392 FYRLG 396
gi 14601825 403 FYRIG 407
gi 41615306 332 FYRVG 336
gi 21243020 213 MGRVG 217
gi 50121553 233 CSRVG 237
gi 17484080 232 MSRVG 236
gi 19886567 397 FYRLG 401
gi 54030425 239 TKYGS 243

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