1JJ2,1YJN,1S1I,1Y69,2HGU,2AW4


Conserved Protein Domain Family
Ribosomal_L16_L10e

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cd01433: Ribosomal_L16_L10e 
Click on image for an interactive view with Cn3D
Ribosomal_L16_L10e: L16 is an essential protein in the large ribosomal subunit of bacteria, mitochondria, and chloroplasts. Large subunits that lack L16 are defective in peptidyl transferase activity, peptidyl-tRNA hydrolysis activity, association with the 30S subunit, binding of aminoacyl-tRNA and interaction with antibiotics. L16 is required for the function of elongation factor P (EF-P), a protein involved in peptide bond synthesis through the stimulation of peptidyl transferase activity by the ribosome. Mutations in L16 and the adjoining bases of 23S rRNA confer antibiotic resistance in bacteria, suggesting a role for L16 in the formation of the antibiotic binding site. The GTPase RbgA (YlqF) is essential for the assembly of the large subunit, and it is believed to regulate the incorporation of L16. L10e is the archaeal and eukaryotic cytosolic homolog of bacterial L16. L16 and L10e exhibit structural differences at the N-terminus.
Statistics
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PSSM-Id: 238714
View PSSM: cd01433
Aligned: 155 rows
Threshold Bit Score: 54.0348
Threshold Setting Gi: 11496540
Created: 12-Dec-2003
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:23S rRNA interface [nucleic acid binding site]
Evidence:
  • Structure:1JJ2_H; Haloarcula marismortui ribosomal protein L10e binds 23S rRNA (1JJ2_0), defined using 3.5 A contacts
    View structure with Cn3D
  • Structure:1Y69_K; Deinococcus radiodurans ribosomal protein L16 binds 23S rRNA (1Y69_0), defined using 3.5 A contacts
    View structure with Cn3D
  • Structure:2HGU_P; Thermus thermophilus ribosomal protein L16 binds 23S rRNA (2HGU_A), defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1        # ##  #                                         ##  #  #   #  #          ### # #
1JJ2_H        37 GAGPTYPAq--------------------------VELVVEKPvQIRHNALEAARVAANRYVQNsgaaanYKFRIRKFPF 90  Haloarcula mari...
NP_038186      4 KYKKQQKGkfynrinskp-------inifnknkynLCLRAKDFgKLTINEIKAFKQNLIKKLKKn----fVLVRFYFNPI 72  Chrysodidymus s...
NP_066424      8 KYTKEQKGknfkkintgq--------gilkqkrnqIYLKALSSgRVSEKNLLACKQTINKIIKKq-----GRLNIHIFAN 74  Ochromonas danica
XP_391953     71 RGPELFHNtll---------------------hkqYGIIATGGgRMKFSHFEMIRMTILRNIDYnk---vFALWRVPSPW 126 honey bee
AAH86509      49 TATKFSQGq--------------------------YGILALGGgFLHWGHIEMMRLTINRSIDPcs---mFAVWRFQPPH 99  zebrafish
NP_060310     77 TEATEFTEg-------------------------nFAILALGGgYLHWGHFEMMRLTINRSMDPkn---mFAIWRVPAPF 128 human
NP_525041     53 RQPQLPPNirppkmqkrlrymrgpemvhntllhkqYAIVATGGgRLRWGHYEMMRLTIGRKMNVnt---mFATWRVPAPW 129 fruit fly
NP_001090501  63 KVNKEPKNlkdirgpak---------egqefeegeYGIVALGGgYLHWGHYEMMRLTLNRALSPrt---iFSRWLVAPPH 130 African clawed ...
XP_426396     87 VATNFTQGq--------------------------YGILALGGgYLHWGHLEMIRLTIGRCMDPkn---mFAIWRVPAPY 137 chicken
NP_497965     51 RGVEEVHTeli---------------------heqYGLAAVSGgFIDSDDFKFIQDRINKNLIDn----qFSVWRVDAPW 105 nematode
Feature 1           ##               #####             #                  ##  ##             
1JJ2_H        91 HVIREnkaaaaaaaaaaaDGMRAPFGKPVGTAARVHgaNHIFIAWVNp-dPNVEEAWRRAKMKVt-----PTINID 160 Haloarcula marismortui
NP_038186     73 TPITKkp---------ieVRMGKGKGSVNYFVMKIKpgTMLCKIECLn-fTIAKKTLMQAKIRLs-----IKTQIY 133 Chrysodidymus synur...
NP_066424     75 TPITKkp---------teIRMGKGKGAIDSWVCRVKpgTILFEINIKf-kIIGFKAFKLIKNKLp-----IKTKII 135 Ochromonas danica
XP_391953    127 QPITMks---------hgLRMGSGKGSINHYVTPVKakQVILEVGGNieyFEVKNILKNIAKRLpfdamaVSQEIM 193 honey bee
AAH86509     100 KPITRks---------lgQRMGGGKGAIDHYVTPIKcgRLVLEMGGQlelVQVEAMLKQVAKKLp-----FPAKVV 161 zebrafish
NP_060310    129 KPITRks---------vgHRMGGGKGAIDHYVTPVKagRLVVEMGGRcefEEVQGFLDQVAHKLp-----FAAKAV 190 human
NP_525041    130 QPITKkg---------qgQRMGGGKGAIDHYVTPIKagRVIVEIAGKcefVEVKQFLQQVANQLp-----FQATVV 191 fruit fly
NP_001090501 131 KPVTRkg---------lgQRMGGGKGAVDHYVTPVKadQLILEVGGRcefQEVQPILSQVAKKLp-----FPAQAV 192 African clawed frog
XP_426396    138 KPLTKks---------lgHRMGGGKGPIDRYVTAVKsgRLVVELGGRcefEEVKPFLTQVAHKLp-----FPAVAV 199 chicken
NP_497965    106 LPRTKka---------qgTRLGGGKGSIQKYVTPVRanRIILEVGGHiteIEAKAFLLYLCERFs-----FPVEFV 167 nematode

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