1K1D,1GKP,1NFG,1KCX


Conserved Protein Domain Family
D-HYD

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cd01314: D-HYD 
Click on image for an interactive view with Cn3D
D-hydantoinases (D-HYD) also called dihydropyrimidases (DHPase) and related proteins; DHPases are a family of enzymes that catalyze the reversible hydrolytic ring opening of the amide bond in five- or six-membered cyclic diamides, like dihydropyrimidine or hydantoin. The hydrolysis of dihydropyrimidines is the second step of reductive catabolism of pyrimidines in human. The hydrolysis of 5-substituted hydantoins in microorganisms leads to enantiomerically pure N-carbamyl amino acids, which are used for the production of antibiotics, peptide hormones, pyrethroids, and pesticides. HYDs are classified depending on their stereoselectivity. This family also includes collapsin response regulators (CRMPs), cytosolic proteins involved in neuronal differentiation and axonal guidance which have strong homology to DHPases, but lack most of the active site residues.
Statistics
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PSSM-Id: 238639
View PSSM: cd01314
Aligned: 29 rows
Threshold Bit Score: 527.171
Threshold Setting Gi: 15921257
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetetramer
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                        # #            
1K1D_A        3 KIIKNGTIVTA--------TDTYEAHLLIKDGKIAMIGQNLEekg--aeVIDAKGCYVFPGGIDPHTHLDmplgg----t 68
gi 27378444   8 LIIRGGRVATT--------TDVFEADVAISGETIAAVGRGLPaa---krEIDARGKLVLPGGVDSHAHIEqlsaa---gi 73
gi 7769065    6 LIIKNGIICTA--------SDIYAAEIAVNNGKVQLIAASIDpsl-gseVIDAEGAFITPGGIDAHVHVDeplkl---lg 73
gi 23113183   4 LIIKNGTVVSP--------SSSTICDVAIKDDKIVGLGFYEAaeg--irTIDATGKYVMPGVIEAHMHCMapfqg----c 69
gi 15921257   1 MIFKNARVITP--------KGIIETDFEVEEGKIKKIKKDIVg-----eGKDLSGYYVLPSVIDGHTHFNsrylgakeii 67
gi 22957516   5 TVIHGGTIVTP--------TESWQGDLGLVGGRIAGLAERLPgg---arRIDATGRLVLPGGIEAHAHIAqesss---gl 70
gi 15921373   5 LILKNVKAFTV--------SGPFEGDIAIKDGKIAKIGGDIQeqa--nkVIDLTGKYVVPGLIDGHTHMEfpfmk----e 70
gi 40747884   6 LIITNATIVQPlslpatasDILPNTDIAISGGKIYLLGQNLSslfptapTLSADGAYVLPGGVDSHVHLQqdns------ 79
gi 22983596  73 TVVRNARVVTA--------ADTFTSDIGIRDGRIVALGLTLDag---vrEIDAAGRHVTPGGVDSHVHFDqptgd---gs 138
gi 27378406  23 LAIRGGTIVTA--------SDEFRADIGIRDGRIVSIADHLEga---arEIDATGLLALPGGIDSHVHISqasgp---dv 88
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1K1D_A       69 vtkDDFESGTIAAAFGGTTTIIDFCLtnk----gepLKKAIETWHNKAngk-avIDYGFHLMISEitd-dvLEELPKVle 142
gi 27378444  74 mnaDTFESATVSAAFGGTTTVIPFAAqhv----gmkLPQVVEDYHALAkkg-avIDYAFHMIIADatretvEEHIPALvk 148
gi 7769065   74 dvvDTMEHATRSAVAGGTTTVVAFSTqdvskkgpsaLAESVKLDVDEYseqtlyCDYGLHLILFQie--kpSVEARELld 151
gi 23113183  70 lgaNTFYQQSVSGAFGGVTMFMDFANvfp----gksVLEAVKERRAEMee--saIDFSVHGKFVKspp-elVEEIPQLae 142
gi 15921257  68 ptaDDYKSGSEITLAGGITSIINFIDpln-----reVTEAVKDEIEKAkst-agIDYSFHLIIKRkd---qINYLPEIik 138
gi 22957516  71 msaDDYYTGSVSAAFGGNSSFIPFAAqhr----gqsVDAVIETYDSRAapn-svLDYSYHLIISDptetvlTEELPRAfa 145
gi 15921373  71 vtaDDFYYGTRAAVAGGVTTIVDFITpak----gqdLLSAYEQWRSNAdpk-viSDYGLHMIIREsnt-kiLEQIPEIin 144
gi 40747884  80 ptgDTWETGTRSAIAGGTTTVLAFASqkrt---dgsLFPVVEEYHRRAsgn-afCDYGFHLILSNptekilAEELPVLvk 155
gi 22983596 139 imaDDFLSGTTSAACGGTTTVIPFACqqr----ghtLREAIDDYHRRAggk-plIDYAFHLIITDptpqvlKEELPTLia 213
gi 27378406  89 vmaDDFASATRAAAAGGNTMVLPFALqek----gtsLRTCVENYRKLAege-cyIDTAFHLIISDptavvlGQELPALvk 163
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      #                                 #                              
1K1D_A      143 --------eeGITSLXVFMAYKnvfq-addgTLYCTLLAAKELGALVMVHAENGDVIDYLTKKAladgNTDPIYHALTRP 213
gi 27378444 149 ---------qGHASIKIFMTYDrlkv--ddePLLDILLAARQSGAMLCAHAENHGIIAWMVKRLlargYTLPKYHAVSHA 217
gi 7769065  152 vqlqaayndyGVSSVKMFMTYPglqi--sdyDIMSAMYATRKNGFTTMLHAENGDMVKWMIEALeeqgLTDAYYHGVSRP 229
gi 23113183 143 ---------yGVPTFKMFMTYKkegvmideeTMLKVFEKAKEVGGLPMLHCEDNTMAEDAIEKVkkigDLSWVNFAKTKP 213
gi 15921257 139 ---------mGIKSIKMFMAYKgsmq-vddeTIYLVMKKAKELGVTVAIHAENGDVIEVLHNEYk--dKKDAIYHALTRP 206
gi 22957516 146 ---------rGITSFKVFMTYDlmnl--gdrGMLDILTVARRHGALTMVHAENNDMVKWMNARLaaagLTAPKYHAISRP 214
gi 15921373 145 ---------kGVVSFKLFMAYKnefm-lsdgELYKLIKRINDFGGVIGIHAENGEIINELIQQFvsegKIEPIYHYYSRP 214
gi 40747884 156 --------eeGISSVKLYMTYQpmrl--rdsELLDVMGTTRSLGMTTMIHAENADMIDWMTKRLesqgRTEPYAHALARP 225
gi 22983596 214 ---------eGYTSFKIYMTYDalkl--sdrEMLDTLSVARNEGAMVMVHAENADCIAWLTERLleagHTAPRYHATSRP 282
gi 27378406 164 ---------dGYTSFKVFMTYDdlvl--sdkQLLEVFDVARREEALVMVHCEGYDAIRFLTTKLeregHIAPYYHGVSRP 232
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #                                                    
1K1D_A      214 PELEGEATGRACQLTel--agSQLYVVHVTCAQAVEKIAEARNKGLDVWGETCPQYLVLDqsyle--------------- 276
gi 27378444 218 RVSEAEAFTRLIGMAal--idQPIMIFHVSTAEGAKVIRDSRGQGLKVFAETCPQYLFLTaadld--------------- 280
gi 7769065  230 SIVEGEATNRAITLAtt--mdTPILFVHVSSPQAAEVIKQAQTKGLKVYAETCPQYALLSdaitrchhhgevesygvgid 307
gi 23113183 214 QKCEAAAFERACRLAey--vdCPVMVVHTTHKEALDVARRAHESGFPIYVETGPHYLTLFddny---------------- 275
gi 15921257 207 VEVEEEAVNRASMLAyl--tgAKTYIVHISSPTSLDIISYWRKKGAKIFSETCPHYLLFDdsyy---------------- 268
gi 22957516 215 ALAEAEAINRAISLArl--vgAGLFIVHVSTPEGADLVARAQASGLPIHAETCPQYLAFTrddld--------------- 277
gi 15921373 215 DIMEIEATNRIASIAsmlgkdVKMYIVHTSTGEAVDIMSSYRKQGFKFYNETVPHYLTLNtdfl---------------- 278
gi 40747884 226 NIAEDEATYRALSLAel--adVPILIVHMSSSVAAKHVRRAQTKLLPVHAETCPHYLFFTseklk--------------- 288
gi 22983596 283 MLVEREATHRAIAFAel--idVPILIVHVSGREAVEQIRWAQSHGLKVYGETCPQYLFLTadslg--------------- 345
gi 27378406 233 QAVEREATHRAISHAei--vgVPIMIVHVSGREAMEQVRWAQQRGLPVHAETCPQYITLTaddmkg-------------- 296
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                          #                            
1K1D_A      277 ------------kpnfEGAKYVWSPPLRek-whQEVLWNALKngqLQTLGSDQCSFDf--------------------kG 323
gi 27378444 281 ------------kpgaDGAKWMCSPPPRth-adQEALWQALSlgdLQTISSDHAPYRfd-------------------eT 328
gi 7769065  308 lssisespftnpddrfIGSKYICSPPIRpe-gtQKSIWKGMNngtFTIVGSDHCSYNyyek---------------tstA 371
gi 23113183 276 -------------ekeEGYLYLCSPPLRtp-qdAEDLWQGLQdgtISVTGSDDCTFDtnekaa----------flekdeN 331
gi 15921257 269 -------------lrsDGNRFIMSPPLRrk-elREELVRKLHm--VNTLGSDYSGFMs---------------------V 311
gi 22957516 278 ------------rpgmEGAKYICSPPLRda-atQAALWNHARrgtFESVSSDHAPYRf--------------------dA 324
gi 15921373 279 -------------krpDGYRYVMSPPLRsd-eqRTKLWMRLAsgdIFTVGSDHCVYSd---------------------A 323
gi 40747884 289 ------------gedfRGAMCVCSPALRespmdLKAMWDGLVngtFTTFSSDHAPSKyiflpsllhqlptvaptrfdhqL 356
gi 22983596 346 -----------cddsfEGAKCICSPPPRdk-anQQVIWDGLEngtFEVFSSDHAPFRyd------------------gpD 395
gi 27378406 297 -----------lnmdiTGAKYVCSPPPRda-esQQAIWEGITsgvFQTFSSDHCPFRyd------------------dpK 346
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1K1D_A      324 QKELGr-------gDFTKIPNGGPIIEDRVSILFSEGVKkGRIT-LNQFVDIVSTRIAKLFglFPKKGTIv-vGSDADLV 394
gi 27378444 329 GKLRAgp-----npNFKQVANGLPGLELRLPLLFDAMVSkGRLG-LEKFVELTATAPAKIYnlHPRKGSIv-vGADADIA 401
gi 7769065  372 SKHRAfdpennkngEFRYIPNGLPGVCTRMPLLYDYGYLrGNLTsMMKLVEIQCTNPAKVYgmYPQKGSIlpgVSDADLV 451
gi 23113183 332 GKYIQ---------DFTKVVNGMSGLEVRLPILLSEGAGkGRLT-INQVCALTSTNVAKIYgcYPQKGIIa-pGSDADLV 400
gi 15921257 312 YKDKAl--------SYIEVPNGVSSTEFLVPTIMSFLFD-NLIT-PEKVAEITSYNQIKLYn-LKEKGFDe--GKDADFT 378
gi 22957516 325 SGKFAnga----epAYPAIANGLPGIAMRLPYLFSEGVAaGRIS-LQQFAALSSSNAARLFg-MERKGALl-pGYDADIA 397
gi 15921373 324 QKKRYree----vpPFHEIPNGVPGTETILPILFYYGVKkGIIG-MERFIEVTSYNPARLFglYPRKGTIm-pGSDADFA 397
gi 40747884 357 GKKKGt-------sSFTQIPNGLPGLETRMPSLFCAGVLtGRLS-VQKFVELTASNPAKLYglSDRKGTIa-pGYDADLV 427
gi 22983596 396 GKRVHgd-----nvSFDKISNGIPGVETRMALLWSEGVRtGRIT-AQSFVALTSTNAAKLYglYPRKGSIa-iGADADLV 468
gi 27378406 347 GKLTPns-----rtSFRWVPNGIPGVETRLPILFSEGVSkGRIS-LQKFVELTATNHARIYglYPRKGSIg-vGFDADIV 419
                       490       500       510       520       530       540       550
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1                                                                                  
1K1D_A      395 IFDPNIERVISAethh-----------------mavDYNAFEGMKVTGEPVSVLCRGEFVVRd--KQFVGKPGYG 450
gi 27378444 402 IWDPNRETVIADemmh-----------------dlaGYTPFAGRKVKGWPVSVLSRGRVIVEg--NKCLASAGSG 457
gi 7769065  452 IWYPDDSKKEYNskpklitn---------klmehncDYTPFEGIEIKNWPRYTIVKGKIVYKe--GEILKENADG 515
gi 23113183 401 IVDMAQEVTLSKdilh-----------------nniSYCLHEGFKVKGYPIMTIARGKVIVEn--GEFRGEKGAG 456
gi 15921257 379 VIKREEWIVKDWhg--------------------kmDYSIYEGVKFKAKVIQTYLRGELTFD---EDYKGSRGKL 430
gi 22957516 398 IWNPEETREVSLadqh-----------------damDYTPFEGMRLTGWPEHVLSRGETVVEa--GELKAARGRG 453
gi 15921373 398 VIDPNRKVRISAdvlh-----------------sniNYTIYEGMEVEGWNIMTIRRGEIVYEe--GQVIGKKGSG 453
gi 40747884 428 IWYPTAEQAEAMqagsssrvtmksfqlknemlhhdiDYTPFEGMEFTNWPRYTILRGKLVWDrdgGGVIGGKGDG 502
gi 22983596 469 IWNEGGEYPVENtrlh-----------------hnvDYTPYEGMRLTAWPAITLSRGDIVWDg--DRPCGETGRG 524
gi 27378406 420 LWDPKLKKPIQQadlh-----------------hgaDYTPWEGFDVTGWPVTTVARGRVVYEh--GRIVGDKGAG 475

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