Conserved Protein Domain Family
Met_dep_hydrolase_A

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cd01299: Met_dep_hydrolase_A 
Metallo-dependent hydrolases, subgroup A is part of the superfamily of metallo-dependent hydrolases, a large group of proteins that show conservation in their 3-dimensional fold (TIM barrel) and in details of their active site. The vast majority of the members have a conserved metal binding site, involving four histidines and one aspartic acid residue. In the common reaction mechanism, the metal ion (or ions) deprotonate a water molecule for a nucleophilic attack on the substrate. The function of this subgroup is unknown.
Statistics
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PSSM-Id: 238624
View PSSM: cd01299
Aligned: 32 rows
Threshold Bit Score: 236.421
Threshold Setting Gi: 6141557
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
active site
Feature 1:active site [active site]
Evidence:
  • Comment:active site is based on homology to other members of the amidohydrolase family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           # #                                                         
gi 6141557   43 AEIIEa--hGKTLLPGLIDSHTHAKp------------------------------------pALEMALTFGVTTELDMF 84
gi 16950585  29 AVTSEe--iRGYAYPGLLDAHTHPGmrrtpepl-----------------------alseirrRLAVLARHGITTVRDAG 83
gi 23102978 155 ARELDl--sGRSVLPGLISNHSHLGmtdgtgagpq-------------------nynrgnierQLRQFQRYGVTTVTSLG 213
gi 21222592  41 CEVVDg--sGATLLPGLIDAHVHSAp------------------------------------gSLALALRFGVTTELEMQ 82
gi 40744031  95 VRVFYg--nGRTLIPGLGDAHTHLSwnggdlgrlgel---------------gveehtlltarSARCFLDSGYTMCFGAA 157
gi 28210644  45 YEKIDi--tGKIIMPGLIDTHLHFSgnrtdndtewvl--------------dpviqktivavaQAREALEHGLTSVGEIS 108
gi 15607216  56 MNLVDl--gESTLLPGLVDAHAHLCwdpdgrpedlag--------------dphavlvgrarrHAAAALRSGITTIRDLG 119
gi 23466009  57 YHYLDg--tGKIVMPGLINAHTHLFsqgkplnpklatpkgqrmvatfahsplgkpymaatvkhNATTLLESGVTTIRTLG 134
gi 26989913  44 PQPGDreahGQFVMPGLVDVHTHLAfgnaqseedidiw-------------tsdefralrglfFAQHVLAAGVTSMVCPG 110
gi 33601694  50 HTVVDl--pGRTIMPGLIDAHTHISfgesrseeenaly-------------tpvefrtakalwYARKVLQAGVTSAFDAA 114
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
gi 6141557   85 Sdpawmt--eqrreAQTRNSMADVRSAMTgaSVAGGHPtglvgv---------vfdkqcPVLENAEDAEAFVAARVAEGA 153
gi 16950585  84 Gqhd--------pkGAWEPGLARVMHSGQhiARFKRYEry------------------lAVDVEPADLPAEAVRQFEKGD 137
gi 23102978 214 Lngplfq-rlrdeaRAGIGVGADLFGADRgiGVPEGAPpmnva------------pdqlYRPATVEEARAAVREMAARKP 280
gi 21222592  83 Gmntre----nrgsIADDDTLADVRSSGFaiTPPGGHPselmpegfrpkwdlppvmplmPFSTTPEEAAAYVPQLLARGS 158
gi 40744031 158 SakrrldvvvrdaiNAGDIPGPRYLANGQemARRDGDLvpg----------------itAYADGPEEMREVIREHVKLGV 221
gi 28210644 109 Rsgiyi----rnmiEEGVIPGPRVVTTGLgfCRTAGHGdshklpldy----nndshpwaERVDGPWDLRKAIRRRIRENP 180
gi 15607216 120 Drdyaal-alreeyRQKTTVGPELVVSGPplTRSGGHCwf-----------------lgGVADSVEELVDAVQERAARGA 181
gi 23466009 135 Dvgyevv-tlrdqiDAGQILGPRILASGPlmAIPEGHGapl----------------iaLTSGTPEEARTAVAQNLKAGV 197
gi 26989913 111 Dsgqlsi-avrntvNAGLFEGPRIAASSRviTNRQSLNdwfptrvg------apeyftaALVTSRDDAIAQIRKQAKDGV 183
gi 33601694 115 Tsyniaq-avrdaiDTGMFEGPRFTVSGKqlTNHQGLEdsfpnema------fppgqsaVLVKTREDLVEAIRYQVKDEV 187
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                     #                   #             
gi 6141557  154 DYFKLVIEDGTIlghevpr-----lsdemvYALVAAAHaqglMAVAHAMTvelallAIRAGVDGLMHVagdrlmtde--- 225
gi 16950585 138 GWIKIVGDWIDRsvgdttp----lwprqalIDAVAGVHdvggKVTVHTFAtetvddLLEAGVDGIEHGtgmtrdh----- 208
gi 23102978 281 DLIKIWVDDFNHtlaykm-------kpeiyTAVIDEAHahglRVAAHVYYlddakkLIAAGADILAHGvrdlpldde--- 350
gi 21222592 159 DFIKFMIDDGTVeghpglp----sldratlNAGVAEARkygaLTVAHALTveatrmAAEAGVDGVTHVfmdqphtae--- 231
gi 40744031 222 DQVKLSMSGESIteirdamd--cyfsdeetKACVDEAHkhgiRLCAHARArdsvrqCIKHGVEIIYHGsyideevtaa-g 298
gi 28210644 181 DAIKIWSTGGGIwrldakad--lhycmeeiQAVVDECNmvglPVWSHAEGyegalnSCKAGVSAIIHGqelneec----- 253
gi 15607216 182 DWIKVMATGGFVttasdpwq--pqygsgqlAAVVAAAEqvglPVTAHAHAtagiaaAVAAGVDGIEHCtflsegsaaasp 259
gi 23466009 198 NAIKIAATGGVTdaqeigeagspqmsveqmRAICDEAHqygvIVGAHAQSpegvrrSLLAGVDTIEHGsvlddeligm-f 276
gi 26989913 184 DLIKIAMDGTHRrpngeiia---aftadetREMVYEAHrlgcRVATHAYGreavmcAAKAGVDLVFHAfymddac----- 255
gi 33601694 188 DAIKVSGSNDNLitpdalgs--saftyeefKIIADETHrlgrLCSVHARSrdaalyAAQAGFDNLFHAsyiddeg----- 260
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
gi 6141557  226 -evaevaqsGIFVVPTLstmasvy-gdkhgqHLAEDDrtrpllsdawaenlcq-----------cwhltnpgstanvidA 292
gi 16950585 209 --lleaaarGILVTPTVhqirrfp-efaakgARFATYverm----------------------------------lgmdA 251
gi 23102978 351 -lieaikarDAWYISTLdldeafy-ifatqpEWTRTPffrqalqpalaaqlddpawrrqtladpkklaiweqgvktnqaN 428
gi 21222592 232 -iidlirdaGMFVIPCItlnasm--mgvtgsELADDPrvaarlddswdrtlrs-----------sydrypqgsledvcaT 297
gi 40744031 299 mdalekkrsQHVVVPAInwlyatl-yeagafGYATEAaekvgykk---------------------------eleaairG 350
gi 28210644 254 --leimkekDITFCPTLqffye-----wftvYEPPYRaihdkypgknt---------------------aekelnriidN 305
gi 15607216 260 dvveaivaqGVWCGMTIp------------rVYPEMPenlvav------------------------------vqdgwrN 297
gi 23466009 277 rhnpnalrgYSALIPTLsaglpltllgqdvtGITDIQlensknv-----------------------------vggmvsG 327
gi 26989913 256 --iealleaGSVLAPTMtfpqnt--vdfcqpHDPAIStgyaayca--------------------------rtlelgapV 305
gi 33601694 261 --ieaclknGCVITPTLtllvnlveakqshaGASSAQafrqei-------------------------------eaasvN 307
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           #                                                           
gi 6141557  293 TRRLYe-----aGVDVLAGSDavtvg--vpgtaHGVSMHGELELLVDcgl-spaAALRAATLLPAKRfsl--ddrGRIAP 362
gi 16950585 252 VRREHlalmvevGTHFLMGSDtae-------nvAEVNLVDELIDAVDdgm-padVVMAAASYAGRARig-----fGVWSA 318
gi 23102978 429 LKRLVe-----aGAKVGFGTDsgat----plriPGFAEHRELQLAVDagl-tplQAIALATGNAAALlgl--ddrGVIAP 496
gi 21222592 298 VRALDa-----aGVDVLAGTDvsmpetffgglaHGASLHHELQYLVAagl-tpsRALRAATATTARRfrl--sdrGRVAE 369
gi 40744031 351 LREMHr-----rGIVVLPGGDygf------awtPHGTYARDLEHFTKllgftphEAIIAATYGVAKLfmrs-hemGQIKA 418
gi 28210644 306 LQNANk-----kGIRITVGSDsfcs----sltpYGEYAITEMYTLNKagl-tkmETILAATKNGAEMlrid-ditGTLEE 374
gi 15607216 298 IRRLId-----aGARVALSTDagva-----pgrRHDVLPDDLVYLSRhgf-tstEVLTGATAAAAAScglg-hrkGRIAP 365
gi 23466009 328 ARQAHe-----aGLMIGVGTDtgm------tfvPQYATWRELELLVAyagfspaEALHAATAVNASIlgvd-aetGSLEV 395
gi 26989913 306 LKRAKa-----aGVPFACGSDsgfa-----vtpYGEWHARELELLVRhldftpaEALFAATHVGARLmprg-etlGTLEP 374
gi 33601694 308 LRRAYd-----aGVPLLAGSEsgws-----pvpYGHWHAREMEIFVKllgltplQAIHANTLACTRLlarhghevGKLEA 377
                       410       420
                ....*....|....*....|
Feature 1                           
gi 6141557  363 GLQADLVLVDGDPTTDVRQT 382
gi 16950585 319 GEPADVVIYREDPERNIETL 338
gi 23102978 497 GKRADLLVVAGDPSQTIADS 516
gi 21222592 370 GLRADLLLVDGDPTGDIGDT 389
gi 40744031 419 GNFADCVLVDGDPLDDITVL 438
gi 28210644 375 GKFADLLVLSKNPLEDIHNV 394
gi 15607216 366 GYDADLLAVAAGVDHDPAGL 385
gi 23466009 396 GKSADLLVLNANPLDDLRAL 415
gi 26989913 375 GKQADLLVLDGSPLDDIRIL 394
gi 33601694 378 GRLADVLVLPGNPLEDITLL 397

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