1GKI


Conserved Protein Domain Family
TrwB

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cd01127: TrwB 
Click on image for an interactive view with Cn3D
Bacterial conjugation protein TrwB, ATP binding domain. TrwB is a homohexamer encoded by conjugative plasmids in Gram-negative bacteria. TrwB also has an all alpha domain which has been hypothesized to be responsible for DNA binding. TrwB is a component of Type IV secretion and is responsible for the horizontal transfer of DNA between bacteria.
Statistics
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PSSM-Id: 238547
View PSSM: cd01127
Aligned: 8 rows
Threshold Bit Score: 543.49
Threshold Setting Gi: 4680167
Created: 10-Jan-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Comment:contains Walker A and Walker B motifs
  • Structure:1GKI; E.coli TrwB bound to MgADP
    View structure with Cn3D
  • Comment:two of the arginine residues are involved in the active site of a different monomer
  • Comment:no evidence of NTPase activity

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                       #######         
1GKI_A       15 KRFLRGTRIVSggklkrmtr---------------ekakqvtvaGVPMPRDAEPr-HLLVNGATGTGKSVLLRELAYTGL 78
gi 27358652 146 DIHIRGFRLAPpeaitkklkarakakk--krgygdgsissfkidGHALLKQYFEvqHLLIDGTTGAGKSIMIRKLVRWIR 223
gi 18077218  85 QQWLRGSRIRNwhfverkvsarnaktnrerrkkgqekaepimigKLPMPLHLEDr-NTMICASIGAGKSVTMEGMIASAL 163
gi 730997   144 NEVTGGRQLTDnpkdvarmlkk------------dgkdsdirigDLPIIRDSEIq-NFCRHGTVGAGKSEVIRRLANYAR 210
gi 22959269 146 NEHVRGSRLIDqrdlarwsmrkwkayq-krfglglkkgprytiaGIPFPPNAVEa-QTGIFGTVGVGKTNAIKELLTTIR 223
gi 46447075 142 KHHLKGNTLSSvwltrwklkl-------------srraspisigSLPLIKGTETq-HIMITGGTGSGKTNCLHHLLKSVR 207
gi 4680167   87 KKIYRGTELVRartladktre--------------rgvnqltvaNIPIPTYAENl-HFSIAGTTGTGKTTIFNELLFKSI 151
gi 10956918 198 AERMPGKTMDDvmkmsfaerka------------agihhvyniaGVSFPWRSEQa-HTIMIGSTGTGKTTQMRDMIAQMR 264
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1GKI_A       79 LRgDRMVIVDPNGDMLSKFGr-dKDIILNPYDQRTKGWSFFNEIRNDYDWQRYALSVVPrgktDEAEEWASYGRLLLRET 157
gi 27358652 224 AQgDKAIIYDKGCTFTSKFYnpaTDVLLNPFDKRCANWDVWSDAIEAPDFENMAAALIPqh-gEGDPFWVDSARTIFSST 302
gi 18077218 164 RRnDRMAVVDPNGTFYSKFSf-kGDYILNPFDARSAGWTIFNEIRGVHDFNRMAKSIIPpqvdPSDEQWCAYARDVLSDT 242
gi 730997   211 QRgDMVVIYDRSGEFVKSYYdpsIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIRdg-tKEDPFWQGSGRTIFAEA 289
gi 22959269 224 AQnGRAIIYDRMGGLVRDFYdpaTDIIINPFDARSHIWSPFGEASTPEALAQIAEVMIPqrpgQNDPFWSQTARLVFEYS 303
gi 46447075 208 QQkQRAIIVDTTGLLTERYYragKDILLNPLDSRGAPWHPWIECIDKTSYATMAESFIPqslsDSDNYWRTSARIVLGSL 287
gi 4680167  152 IRgGKNIALDPNGGFLKNFYr-pGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQespdMATEEWLAYGRLIFSEV 230
gi 10956918 265 VRqDRAVVFDLTGAYVEAFYnpeTDTILNPMDERCPSWSLFDEGKNYADFTAIASAILPtdggGSDPFWMLGARTLFVQT 344
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1GKI_A      158 AKKLALig-tpSMRELFHWttiatFDDLRGFLegtlaeslfagsNEASKALTSARFVLSDKlpehvt-----------mp 225
gi 27358652 303 AYRMSQdnkpcTTERLLSLiltseLETLGNFLegtes--aslvsTDIKKTAISIKSVLATYikslrfldglnhqerpdkl 380
gi 18077218 243 MRKLMEtg-naNQDTLVNLlvredGDTIRAFLantds--egyfrDNAEKAIASIQFMMNKYirplry-----------mg 308
gi 730997   290 AYLMRNdp-nrSYSKLVDTllsikIEKLRTYLvihrq--anlveEKIEKTAISIRAVLTNYvkairylq------giehn 360
gi 22959269 304 ARSLLRak-rpTNADLRRAimtisAEELEKLLagtpg--ahffgKHVEKTSASIRANMIAElrflefl---------rdd 371
gi 46447075 288 FEQLKDi---qKNSTLADKilrdpLQELCDFVqgtka--aslidMNSEKTAASIRAVMSNFltcfell---------ant 353
gi 4680167  231 SKKLHSlystvTMEEVIHWacnvdQKKLKEFLmgtpa---eaifSGSEKAVGSARFVLSKNlaphlk-----------mp 296
gi 10956918 345 CVQLMKlg-qaTNAALAYRlmmadLEEVHELLrntia--epltaPVAARMAESVRAVLNTNaqallfi---------peg 412
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1GKI_A      226 dgdFSIRSWLEDpn----gGNLFITWRedmgpaLRPLISAWVDVVCTSILSLPEepkRRLWLFIDELaslekLASLADAL 301
gi 27358652 381 rqkFSITNWVQDdn---qkGFLFLSSNaqqhasLRPLISMWLAIASNAILGLKEdeeRRIWVIMDEMpslhkLPELGSII 457
gi 18077218 309 kgdFSIYRWVNDpd----aGNLFITWRedmrdtMRPLVATWIDTICATILSSEPmtgKRLWLFLDELqslgkLESFVPAA 384
gi 730997   361 gepFTIRDWMRGvredqknGWLFISSNadthasLKPVISMWLSIAIRGLLAMGEnrnRRVWFFCDELptlhkLPDLVEIL 440
gi 22959269 372 gelFSAREWVKSer----pGFVFLTGDaehsaaTRNIISTIIEVSANALMTCEEsrdPKVWFFLDEVpslnrLPFLQSKL 447
gi 46447075 354 dqpFSIREWIQQedp--idNWLFISCKtneraaLNPLLSCWLSVAVRSLLALPPnfeRRLWYIIDELptlnrLRDLDMLL 431
gi 4680167  297 egnFSLRDWLDDgk----pGTLFITWQeemkrsLNPLISCWLDSIFSIVLGMGEk-eSRINVFIDELeslqfLPNLNDAL 371
gi 10956918 413 kepFSICDWIRHqdk--pgSILFITSShnelvlNRALLSLWMNLAVHTLMRLPRtrsLRTWFFFDEVhalhrLPAIEDGL 490
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #                                                                            
1GKI_A      302 TKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASfRSLVVLGGsrtdpKTNEDMSLSLGEHEVERDryskntgk---hh 378
gi 27358652 458 AEVRKFGGCYLIGIQSYAQLVKTYGKNAAEEMFDLlNTRFFFRAps--ePMARISAKDLGEQEVDVSkenvsygantlrd 535
gi 18077218 385 TKGRKHGLRMVGSLQDWSQLNASYGRDDAETLLSCfRNYVILAAan--aKNADMAKEILGNHEVKRFrkswtag------ 456
gi 730997   441 PEARKFGGCYVFGIQSYAQLEDIYGEKAAASLFDVmNTRAFFRSps--hKIAEFAAGEIGEKEHLKAseqysygadpvrd 518
gi 22959269 448 AEIRQFGGAFVLGYQVFAQLEDIYGEKGAQAIAGNlNNRIVFNTpd--aRTAKLFSESLGFEDVVEQrenlsfgahesrd 525
gi 46447075 432 AESRKYGGCAVLALQSPAQLEAIYGQAVAQTLIGNcATKIVFAEqn--pLHAGRLADMFGEQEIREYqkglsygandird 509
gi 4680167  372 TKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANmRSNIVLGGsrlgdETLDQMSRSLGEIEGEVErkesdpqk---pw 448
gi 10956918 491 QTARGFGGAFVLGIHSFAKLAETYGKEGAQNLASLaRTKLILAAad--rDTAEHCSDYIGHREVRMMdeaysygysnird 568
                       410       420       430       440
                ....*....|....*....|....*....|....*....|
Feature 1                                               
1GKI_A      379 stgralERVRERVVMPAEIANLPdlTAYVGFAgnRPIAKV 418
gi 27358652 536 gvslghQTITRPVVSASEIQALDdlQCYLRSPgiDLITRL 575
gi 18077218 457 rmtraeEIKLEPVVMDSEISNLNdlEAYVKFAedFPITKI 496
gi 730997   519 gvstgkDMERQTLVSYSDIQSLPdlTCYVTLPgpYPAVKL 558
gi 22959269 526 gvafmsQRTERPIVTASEIQALPqfEALIRFAydAPTARV 565
gi 46447075 510 gvslnqQTRYQQLITKTDIQFLSrnQAFVRLPdnCPIVRL 549
gi 4680167  449 ivrkrrDVKVVRAVTPTEISMLPnlTGYLALPgdMPVAKF 488
gi 10956918 569 aatitpRSEVQPLVIPDDIMRLPslRGFLVFPegFDAARI 608

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