1M2O,1F6B,2GAO


Conserved Protein Domain Family
Sar1

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cd00879: Sar1 
Click on image for an interactive view with Cn3D
Sar1 is an essential component of COPII vesicle coats
Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. Activation of the GDP to the GTP-bound form of Sar1 involves the membrane-associated guanine nucleotide exchange factor (GEF) Sec12. Sar1 is unlike all Ras superfamily GTPases that use either myristoyl or prenyl groups to direct membrane association and function, in that Sar1 lacks such modification. Instead, Sar1 contains a unique nine-amino-acid N-terminal extension. This extension contains an evolutionarily conserved cluster of bulky hydrophobic amino acids, referred to as the Sar1-N-terminal activation recruitment (STAR) motif. The STAR motif mediates the recruitment of Sar1 to ER membranes and facilitates its interaction with mammalian Sec12 GEF leading to activation.
Statistics
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PSSM-Id: 206645
View PSSM: cd00879
Aligned: 22 rows
Threshold Bit Score: 257.592
Threshold Setting Gi: 19173546
Created: 4-Feb-2003
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1M2O: Saccharomyces cerevisiae Sar1 binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                  #######               #                     #        
1M2O_B        5 DIFGWFRDvlaslglwnKHGKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELAIGNIKFTTFDLGGHIQARRLW 84
gi 34582431   2 GFGDWFKSalsflglykKKATIVFVGLDNAGKSTLLAMLKNSATTTVAPTQQPTSQELVMGSIRFKTFDLGGHEVARQLW 81
gi 19173546  19 FYEKVFQNfvks--lfgKPSSILFLGIDNAGKTTLVNKLKSDSTDVYMPTHHPSTSYIEIGNLKAQVIDLGGHTAARLAW 96
gi 47028319   3 YFSGFFTGilklfgfysKEATVVLIGLDNAGKTTLQYKLRTGESYSFVPTQKPQEQEITIGNVKLQTWDLGGHKAVRKLW 82
gi 74834470   2 FILNMFKSllgslglykKNAKILFLGLDNAGKTTLLRRLKDDRMVQHDPTLHPHAEELVLGNVRFKAFDLGGHKAVRKTW 81
gi 23394386   2 FVFDWFYNvlgylglyhKNAKILFLGLDNAGKTTLLHMLKDDRVAVHEPTLHPNSEELIIGKLRLRTFDLGGHETARRLW 81
gi 67614753   2 FVFSWFRDilswfglsnKSAKILFLGLDNAGKTTLLHMLKDDRIATHVPTLHPHSEELVIGKIKFKTFDLGGHETARRIW 81
gi 65304190   3 FIINWIKKtlqslgllnKDARIVFLGLDNAGKTTLLRMLKDNRVGIHTPTLHPHSEQLSLEKVNLTAFDLGGHETARRVW 82
gi 46946656   2 FLVDWFYGvlaslglwqKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGRIKFKAFDLGGHQIARRVW 81
gi 66816775   2 FLVDWFYNmflwlgffkKEAKIVIIGLGNAGKTTLLHLLVTGSLKSHIPTLRPNAESFTYGNVNFKAYDLGGQQNLRFLW 81
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                       ## #                            
1M2O_B       85 KDYFPEV-NGIVFLVDAADPERFDEARVELDALFNIAELkdVPFVILGNKIDAPn-----AVSEAELRSALgllnttg-- 156
gi 34582431  82 EQYVTNS-DGIVFLVDSADPSRFEESRRTLQELLDNHDLatTPILILSNKVDIQt-----AVSMETMVQSFgiqhlltgk 155
gi 19173546  97 RDYFYDC-HGIVFIVDVHDVERFQEVREAYETVLSLEKR--APVVVLMNKIDLEghtpetAEADYQWKSWLsqetgienq 173
gi 47028319  83 KQYYRTA-DGIVFVVDAADKSRLPEAKKVLNFILKEDALveTPIAILGNKSDKKe-----AVPMQKLQEELglpkl---- 152
gi 74834470  82 KNYFPTV-DGIIYLVDSADSQRLKESRDELEQILNTAELakVPIVILGNKIDKPg-----AVPEEELRQALginvkqqin 155
gi 23394386  82 RDYFATV-DGVVFVVDALDRERFPESKRELDTLLGYDELanVPFLVLGNKIDVPr-----AASEDELRSALglyetygke 155
gi 67614753  82 KDYFATV-DAIVFLVDASDRSRFGETREELSNLLETPELqnVPFVILGNKIDIPm-----AASEDELRQSLglfehltyg 155
gi 65304190  83 KQYCGNV-DGIVFIVDASDRSRFQESNEELRSLLSDEELtnKPFVVLGNKIDNRg-----AASEEELRTALslyandtyg 156
gi 46946656  82 KDYYAKV-DAVVYLVDAVDKERFAESKKELDALLADDSLanVPFLILGNKIDIPy-----AASEEELRYYLglsnfttgk 155
gi 66816775  82 KQYVPDSkTIIVFMVDSSDYNSIIESKSEIHDILGDEHLsqSPLLILGSKCDAKg-----HHNRENLIDLLdirrfel-- 154
                       170       180       190
                ....*....|....*....|....*....|....*....
Feature 1                          ###                 
1M2O_B      157 ----sqriegqRPVEVFMCSVVm-rNGYLEAFQWLSQYI 190
gi 34582431 156 --ggsnlrsdqRPLEVFPCSVIn-rFGYTDGFKWLSKYL 191
gi 19173546 174 edpergqvvkiFYVTITSGSANsitGPLARAFKWLEAMI 212
gi 47028319 153 -------ldayRKIKVFNTSVTe-gYGYPTAFEWIAANL 183
gi 74834470 156 --nknikeidgRPVDVFMCSVAn-rVGYAEGFRWLSQFL 191
gi 23394386 156 --argdkdasiRPIELYMCSVVr-rMGYADGFRWMAQFL 191
gi 67614753 156 r-gekrgdngiRPVEVFMCSVVr-rMGYSEAFRWLSQFL 192
gi 65304190 157 ---ksvksgrgRPVELFMCSIIk-kQGYAEALRWLSHFL 191
gi 46946656 156 g-nvnladsnvRPLKIFMCSVVr-kMGYGEGFKWMSQYI 192
gi 66816775 155 ----glnnsnnVPFDLIMTSSIt-rYGITDMLNWLDKCT 188

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