1EUQ,2HZ7


Conserved Protein Domain Family
GlnRS_core

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cd00807: GlnRS_core 
Click on image for an interactive view with Cn3D
catalytic core domain of glutaminyl-tRNA synthetase
Glutaminyl-tRNA synthetase (GlnRS) cataytic core domain. These enzymes attach Gln to the appropriate tRNA. Like other class I tRNA synthetases, they aminoacylate the 2'-OH of the nucleotide at the 3' end of the tRNA. The core domain is based on the Rossman fold and is responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate. GlnRS contains the characteristic class I HIGH and KMSKS motifs, which are involved in ATP binding. These enzymes function as monomers. Archaea and most bacteria lack GlnRS. In these organisms, the "non-discriminating" form of GluRS aminoacylates both tRNA(Glu) and tRNA(Gln) with Glu, which is converted to Gln when appropriate by a transamidation enzyme.
Statistics
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PSSM-Id: 185676
View PSSM: cd00807
Aligned: 21 rows
Threshold Bit Score: 402.785
Threshold Setting Gi: 19074040
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1EUQ_A; E. coli GlnRS binds 5'O[N-(L-Gln)sulfamoyl] adenosine, defined using 4.0 A contacts
    View structure with Cn3D
  • Comment:tRNA is required for amino acid activation

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #####     # ## ##                   # ##                                    
1EUQ_A        27 TVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWsg----nVRYSSD 102
gi 17511007  193 KVVVRFPPEASGYLHIGHAKAALLNQYYQQAFEGQLIMRFDDTNPAKENAHFEHVIKEDLSMLNIVPd-----rWTHSSD 267
gi 19074755  197 RVVTRFPPEPNGILHIGHAKAINLNFEYAKKFGGYTYLRYDDTNPKNEEAEYFDSIYEDVRWLGFEPy-----kVTASSD 271
gi 20043025  205 EVCVRFAPEPSGYLHIGHAKAALLNKYFAERYQGRLIVRFDDTNPSKESNEFVENLLKDIETLGIKYd-----aVTYTSD 279
gi 21362946  206 KVVTRFPPEPSGYLHIGHAKAALLNQYFANKYHGKLIVRFDDTNPSKENSEFQDAILEDVALLGIKPd-----vVTYTSD 280
gi 21751193  201 KVVTRFPPEASGYMHIGHVKAAMLNYYYAKRYQGKLLLRFDDTNPSKEKEEFETSIIEDLAKLGIKAd-----lFSHTSD 275
gi 22096197   44 PIITRFPPEPNGYLHIGHAKSIWVNFGLAKEYGGRCHLRFDDTNPVKEDTEYVDSIIDAVHWLGYSWqngtgehLYYASD 123
gi 23489563   80 YVITRFPPEPNGYLHLGHAKSICLNFGLSQKYGGQTHLRFDDTNPVTEDIRYINSIQEDVKWLGFNWne----hLYYASD 155
gi 23490688  275 KVVTRFPPEPSGYLHIGHIKAAFLNNYYANIYEGKMLLRFDDTNPVLEDVKFENSIMEDLKTLGIKYe-----kISYTSD 349
gi 34541554   34 RIQTRFPPEPNGYLHIGHAKAICIDFGIAQRYGGVCNLRFDDTNPVKEDVEYVDAIREDIEWLGFHWg-----nIYYASD 108
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1EUQ_A       103 YFDQLHAYAIELINKGLAYVdeltpeqireyrgtltqpgknspyrdrsveenlalfek-----mraggfeegkaclraki 177
gi 17511007  268 HFEMLLTMCEKLLKEGKAFVddtdtetmrnereqrqdsrnrsntpeknlqlweemk---------kgspkgltccvrmki 338
gi 19074755  272 YFDKMTEFGFQLIRKGKAYVchlsqdeicerrrqyvsdgtndrshlsqyrdrpvsenlrlfqemvdgkweegkaclrfkm 351
gi 20043025  280 YFPKLMEMAEKLIKQGKAYVddtpkeqmrsermdgveskcrnntveenlslwkemi---------ngsergmqccvrgkl 350
gi 21362946  281 YLDTIHQYCVDMIKSGQAYAddtdvetmrhertegipskhrdrpieesleilsemd---------kgsdvglknciraki 351
gi 21751193  276 YFDVILDYARQMIREGLAFMdntdqetmrkermerkesklrntspeenlrlfealc---------rgepevqdyclraki 346
gi 22096197  124 YFEQLYGFAEVLIQRGAAYIdsqsaeqiaanrgdftrpgtpspfrdrsveenlalfrd-----mragkyqdgqhvlrari 198
gi 23489563  156 YFDQLYEWALQLIKQGDAYVddqtieeirknrgslkepginspyrnrsieenlvlfen-----mklgkykegekvlraki 230
gi 23490688  350 YFQLLEEYCIKLIKMNKAYAddtnvdemrnqrgegiesvnrnnsidtnlklfeemrn--------gtdigkkncirakid 421
gi 34541554  109 YFQELYDFAERLIREGHAYVdeqtaeqiaaqkgsptvpgtaspfrdrpaeesldlfrr-----mnvgefeegamtlraki 183
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                          #   #            ###  #  #                    
1EUQ_A       178 dmaspfivmrdpvlyrikfaeHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLYDWVLDNITi-----pV 252
gi 17511007  339 dmksnngamrdptiyrckpeeHVRTGLKYKVYPTYDFTCPIVDSVEGVTHALRTTEYHDRDDQYYFICDALGl------R 412
gi 19074755  352 dtdtknplmldlvgirildvvHPRKNVKYTVYPTYEFALCVSDSLEDVTHSFCTREFYTRQESYNWLLVQLEi------Y 425
gi 20043025  351 dmqdpnkslrdpvyyrcntdpHHRIGSKYKVYPTYDFACPFVDALEGVTHALRSSEYHDRNAQYYRILQDMGm------R 424
gi 21362946  352 syenpnkamrdpviyrcnllpHHRTGTKYRAYPTYDFACPIVDSLEGVTHALRTTEYRDRNPLYQWMIKAMNl------R 425
gi 21751193  347 dmksdngtlrdpvlvryfpltHMRTGDKYKAYPCYDLACPIVDSIEGVTHAMRTTEYKDRDEQYMWIQKALRl------R 420
gi 22096197  199 dmaapnivmrdpvlyrirhahHHRTGDAWCIYPMYDFTHCISDALENITHSLCTLEFENNRPLYDWVLDHLRdagalpaP 278
gi 23489563  231 dmssgninlrdpilyrimnkiHPKTKNKWVIYPMYDFAHGQSDSIEKITHSICTLEFETHRPLYEWFQDKLNi------F 304
gi 23490688  422 mssknkcmrdpvlyrciidipHHRHEFKYKCYPTYDFACPIIDSIEGVTHALRTNEYSDRIEQYNWFISTLEl------R 495
gi 34541554  184 dmassnmhfrdpiiyriikhpHHRTGNEWNVYPMYDFAHGQSDYFEGVTHSICTLEFEVHRPLYNYFIELLRkd----sY 259
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           #    ##        #                                                             
1EUQ_A       253 HPRQYEFSRLNLeytVMSKRKLnllvtdkhveGWDDPRMPTISGLRRRGYTAASIREFCKRIGvtkqdNTIEMASLESCI 332
gi 17511007  413 RPHIWEYARLNMtntVMSKRKLtwfvdeghveGWDDPRLPTVRGVMRRGLTVEGLKQFIVAQGgsrsvVMMEWDKIWAFN 492
gi 19074755  426 KPIQWEFSRLNIsntVLSKRKLlplk--kygiELDDPRLFTIKGMRRRGFPPEAINQFCRSLGftfaeTTVDVKKLENFV 503
gi 20043025  425 RVEIYEFSRLNMvytLLSKRKLlwfvqnkkveDWTDPRFPTVQGIVRRGLKVEALIQFILQQGasknlNLMEWDKLWTIN 504
gi 21362946  426 KIHVWEFSRMNFvrtLLSKRKLteivdhglvwGWDDPRFPTVRGVRRRGMTIEALQQYIVSQGpskniLTLDWTSFWATN 505
gi 21751193  421 PVHLVEFARLNFqytLMSKRKLtwmvehhevdGWDDPRFPTVKGVLRRGVQVEALRAFILSQGfskrvVTMEWDKFWSDN 500
gi 22096197  279 LPHQYEFARLHLtyaITSKRKLlqlvnekrvdGWDDPRMPTLVGIRRRGYTPESIQLFCERVGvskadSWIDMSILEAAV 358
gi 23489563  305 KTRQIEFARLNVsymVMSKRKLltlvnekwvnDWDDPRMPTISGMRRRGYSPDAIKDFCNKVGvakreNMISYELLELCA 384
gi 23490688  496 NVHIYEFSRLAFvktVMSKRKLkwfvennivdGWTDPRMPTIKGILRRGLTKEALFQFILEQGpskagNLMQWDKLWSIN 575
gi 34541554  260 APRQIEFNRLNLtytMMSKRKLlqlvkdglvsGWDDPRMPTLCGYRRRGYTPESIRNFIDKIGytkydGIIDVALLEHAV 339
                        330
                 ....*....|
Feature 1                  
1EUQ_A       333 REDLNENAPR 342
gi 17511007  493 KKVIDPVAPR 502
gi 19074755  504 RDNLNRTSRR 513
gi 20043025  505 KKIIDPVCAR 514
gi 21362946  506 KKIIDPVAPR 515
gi 21751193  501 KKILERNALR 510
gi 22096197  359 RDDLDARAPR 368
gi 23489563  385 REDMNKKAIR 394
gi 23490688  576 KQIIDPNIPR 585
gi 34541554  340 REDLNKRATR 349

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