1QFL,1AFW,1M3K,1M1T


Conserved Protein Domain Family
thiolase

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cd00751: thiolase 
Click on image for an interactive view with Cn3D
Thiolase are ubiquitous enzymes that catalyze the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA, a 2-step reaction involving a covalent intermediate formed with a catalytic cysteine. They are found in prokaryotes and eukaryotes (cytosol, microbodies and mitochondria). There are 2 functional different classes: thiolase-I (3-ketoacyl-CoA thiolase) and thiolase-II (acetoacetyl-CoA thiolase). Thiolase-I can cleave longer fatty acid molecules and plays an important role in the beta-oxidative degradation of fatty acids. Thiolase-II has a high substrate specificity. Although it can cleave acetoacyl-CoA, its main function is the synthesis of acetoacyl-CoA from two molecules of acetyl-CoA, which gives it importance in several biosynthetic pathways.
Statistics
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PSSM-Id: 238383
View PSSM: cd00751
Aligned: 223 rows
Threshold Bit Score: 222.354
Threshold Setting Gi: 50955605
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitedimer interface
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1QFL; Zoogloea ramigera biosynthetic thiolase with bound reaction intermediate
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1QFL_A        3 VIASAARTAVGSFnGAFANTPAHELGATVISAVLEragvaagevNEVILGQVLPagegqNPARQAAMKAGVPqeATAWGM 82  Zoogloea ramigera
P76503       16 AIVSGLRTPFARQaTAFHGIPAVDLGKMVVGELLArseipaeviEQLVFGQVVQmpeapNIAREIVLGTGMNvhTDAYSV 95  Escherichia coli
P34255       27 VLVDAVRTPFVVSgTVFKDLMAVDLQKEAIKALVEktklpyeqlDHIICGTVIQecktsNIAREAALLAGVPdkIPAHTV 106 nematode
ZP_00133854  15 AIVSGLRTPFARKdTGFKDAYATSLGTMVTNELLSrtaiehheiEQLVFGQVIQqpdipNPAREIALALNMPk-LQSYSI 93  Actinobacillus p...
ZP_00187174  13 VLVDGVRTPFMRAgTAYLSQTSYDLARTVLRGLLEragvapesvGYVVMGTVIQnistsNVARDAALAAGLPssVPAHTV 92  Rubrobacter xyla...
AAL39269     45 VLVDGVRTPFLTSgTTYSKLMPHELARHSLLSLLQknrldkeliDYIVYGSVIQevktsNIAREAALAAGFSnkTPAHTV 124 fruit fly
XP_420004    55 VVVEGVRIPFLQSgTSYADLMPHDLARAALQGLLNrtsvprdvvDYIVYGTVIQevktsNVAREAALGAGFSdkTPAHTV 134 chicken
AAH45222     51 VIVDGVRTPFLLSgTTYADLMPHDLARTALQGLLQrtnvprevvDYIVYGTVIQevktsNVAREAALGAGFSdkTPAHTV 130 African clawed frog
AAH46041     52 VLVEGVRTPFLLSgTTYSDLMPHDLARAALQGLLKrtgipkdavDYIVYGAVIQevktsNVAREAALGAGFSdkTPAHTV 131 zebrafish
BAB20046     48 VLVEGVRTPFLMSgTQYKNLMPHDLAREALKGLLKrtemattdvDYIVLGTVIQesktpNIAREAALGAGFSerIPSHTV 127 Polyandrocarpa m...
Feature 1          #                                                                            
1QFL_A       83 NQLXGSGLRAVALGMQQIATGdaSIIVAGGMESMSMAphcahlrggvkmgdfkm-----------------idtmikdgl 145 Zoogloea ramigera
P76503       96 SRACATSFQAVANVAESLMAGtiRAGIAGGADSSSVLpigvskklarvlvdvnkartmsqrlklfsrlrlrdlmpvppav 175 Escherichia coli
P34255      107 TLACISSNVAMTTGMGMLATGnaNAIIAGGVELLSDVpirynrnarkamlgmnkakdvpsklkiggqivknllspelpav 186 nematode
ZP_00133854  94 SSSCLSGLQAIANVAGSIVSGsiSAGIAGGADSISNAplsisprviyklksifnaetle-------ekyrrfrhfswrdl 166 Actinobacillus p...
ZP_00187174  93 TMACISANQAITSALETIRAGkaEAAVAGGVEVMSDTppqfgkkarerlflaqgyrspl-------efrrllsgmspsdf 165 Rubrobacter xyla...
AAL39269    125 TMACISSNAAITTGMGLIATNtyDVIVAGGVEFMSDVpirhsrkmrslllkankaktlg-------qrlallstfrpdfl 197 fruit fly
XP_420004   135 TMACISSNQAMTTGIGLIAAGqcDVVVAGGVELMSDVpirhsrkmrktlltlnraktlg-------qklaliskirpnyf 207 chicken
AAH45222    131 TMACISSNQAMTTGVGLIASGqcDVIVAGGVEFMSDVpirhsrkmrktmlslnkaktlg-------qrlsvisrirpnyl 203 African clawed frog
AAH46041    132 TMACISSNQAMTTAVGLIAAGqcDAVVAGGVEFMSDVpirhsrkmrktmlslnkaktlg-------arlsllgsirlahl 204 zebrafish
BAB20046    128 SQACISSNQAITTAMSLIASGqcQAVIAGGVDFMSDVpirfnrtmrklmldankaktmg------qrlglaskvifspmr 201 Polyandrocarpa m...
Feature 1                                                                                       
1QFL_A      146 tdafygyHMGTTAENVAKQWql---------sRDEQDAFAVASQNKAEAAQKDGRFkDEIVPFIvkgrkgdiTVDADEYI 216 Zoogloea ramigera
P76503      176 aeystglRMGDTAEQMAKTYgi---------tREQQDALAHRSHQRAAQAWSDGKLkEEVMTAFipp--ykqPLVEDNNI 244 Escherichia coli
P34255      187 aefstgeTMGHSGDRLAAAFnv---------sRREQDEFAIRSHTLASEAAKNGKF-TDVVPVFldgk-kpkTIKEDNGI 255 nematode
ZP_00133854 167 kphgvnlRDFTTQMSVAEVSeqmaq--dnhisRAEQDEYARLSNQRASDAWKIGLLkEEVMPSFprp--ykdFVVSDTLI 242 Actinobacillus p...
ZP_00187174 166 lprapaiSEYSTGELMGESAdrlaa--afgvsREEQDEYALRTHTLAAKATESGRLaAEILPTAppp--dfeLLTEDNTI 241 Rubrobacter xyla...
AAL39269    198 apelpavAEFSSGETMGHSAdrlas--afnvsRSEQDEYALRSHTLAKEAQEKGYF-TDLVPFKvsg--vdqIVDKDNGI 272 fruit fly
XP_420004   208 apelpavAEFSTSETMGHSAdrlaa--afaisRVEQDEYALRSHTLAKKAQDEGLL-LDVVPFKvpg---kdTVTKDNGI 281 chicken
AAH45222    204 apelpavAEFSTSESMGHSAdrlaa--afsvsRVEQDEYALRSHTLAKKAQDAGLL-SDIIPYKvpg---reTVEKDNGI 277 African clawed frog
AAH46041    205 apelpavAELSTAETMGHSAdrlaa--afgvsRLEQDEFALRSHTLAKKAQDGGLL-SDVVGFEvpg---kdIVSKDNGI 278 zebrafish
BAB20046    202 ylspelpAIAEFSTNEVMGHsadrlaaafgitRQAQDEFAYRSHSLAHKATEEGKL-SDVLEFKvpg--vadLVKTDNGI 278 Polyandrocarpa m...
Feature 1                                                                                       
1QFL_A      217 RhgATLDSMAKLRPAFDk-eGTVTAGNASGLNDGAAAALLMSEaeasrrgIQPLGRIVSWATVGVDPk-VMGTGPIPASR 294 Zoogloea ramigera
P76503      245 RgnSSLADYAKLRPAFDrkhGTVTAANSTPLTDGAAAVILMTEsrakelgLVPLGYLRSYAFTAIDVwqDMLLGPAWSTP 324 Escherichia coli
P34255      256 Rv-STLEKLSSLKPAFVkphGTVTAANASYLTDGASAALIMTEeyalangYKPKAYLRDYLYVAQDPkdQLLLSPAYVIP 334 nematode
ZP_00133854 243 SaaTRASYYQRFSPIVDkeyATVTEANMPQPMDGAAAVLLMNEsrakalgLMPLGYIRSYAITGNDIwqDMFAGATIAAA 322 Actinobacillus p...
ZP_00187174 242 RrdTSLEKLAALPPAFVkplGTVTAGNSSPLTDGAAATLLMEEdaaraagHEPKARLADYLYVAQDPgeELLLGPAYAVP 321 Rubrobacter xyla...
AAL39269    273 Rv-SSPESLAKLRPAFVkpyGTVTAANASFLTDGASACIIMTEekakqlgLKPKAYLRDFLFVSQDPvnQLLLGPAYGIP 351 fruit fly
XP_420004   282 Rp-SSLEQMSKLKPAFVkpyGTVTAANSSFLTDGASAMLLMSEekalalgYKPKAYLRDFVYVSQDPkdQLLLGPTYATP 360 chicken
AAH45222    278 Rp-SSMEQMTKLKPAFIkphGTVTAANSSFLTDGASAVLIMSEekalamgYKPKAYLRDFVYVSQDPkdQLLLGPTYATP 356 African clawed frog
AAH46041    279 Rp-STMEQMAKLKPAFVkphGTVTAANSSFLTDGASAVLIMSEekalamgYKPKAYLRDFVYVSQDPkdQLLLGPTYATP 357 zebrafish
BAB20046    279 Rp-TPLEKMAKLKAAFIkphGSITAANSSFLSDGGSACLIMEEskakamgLKPKAYLREFTYVSQDPkdQLLLGPAYATP 357 Polyandrocarpa m...
Feature 1                                                                          #            
1QFL_A      295 KALERAGWKIGDLDLVEANEAFAAQACAVNKDLGWDp-----------------sIVNVNGGAIAIGHPIGASGARILn- 356 Zoogloea ramigera
P76503      325 LALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSErfarealgrahatgevddsKFNVLGGSIAYGHPFAATGARMIt- 403 Escherichia coli
P34255      335 KLLDKAGLTLKDVDVFEIHEAFAGQVLANLNAMDSDyfckeqmkrsgkfgrvpmdKLNLWGGSLSIGHPFGATGVRLAt- 413 nematode
ZP_00133854 323 QALDRAELSLSDMDYIDIHESSASQILANIRFFESDdfak------------kqlNRTACLGKIDMDKLNHLGGSIAFgn 390 Actinobacillus p...
ZP_00187174 322 RLLERNGLSLSDIDVLEIHEAFAGQVLAVLRALESDrfar------------erlGLGRRVGEVEMERVNAWGGSVSLgh 389 Rubrobacter xyla...
AAL39269    352 KLLKKAGLTLKDIDSWEIHEAFAGQIVANLKALDSDwfck------------tylGLNEKVGTPDLSKWNNWGGSLSIgh 419 fruit fly
XP_420004   361 KVLEKAGLTMADIDVFEFHEAFAGQILANMKAMDSDwfaqn----------ymgrKSKVGAPPLEKFNTWGGSLSLGHpf 430 chicken
AAH45222    357 KVLERAGLTLADIDVFEFHEAFSGQILSNLKAMDSDwfaqn----------ymgrKAKVGAPALDKFNMWGGSLSLGHpf 426 African clawed frog
AAH46041    358 KVLEKSGLTMDDIDVFEFHEAFAGQIMANLKAMGSDwfaqt----------ymgrKTKVGVPPMEKFNTWGGSLSLGHpf 427 zebrafish
BAB20046    358 IVLEKAGLKMSDIDVFEYHEAFAGQILANLAAMESEyfakn----------ymnrSTKVGAPPIEKFNLWGGSLSIGHpf 427 Polyandrocarpa m...
Feature 1                                         #             
1QFL_A      357 ---------TLLFEmkrr-------garKGLATLCIGGGMGVAMCIES 388 Zoogloea ramigera
P76503      404 ---------QTLHElrrr-------gggFGLVTACAAGGLGAAMVLEA 435 Escherichia coli
P34255      414 ---------HSAHRlkee-------kgqYAVIAACAAGGHGVGMLIEA 445 nematode
ZP_00133854 391 p-----ravTSLRTviqslyalkrkgggISLVASGGLGGLGAAMVLES 433 Actinobacillus pleuropneumoniae serovar 1 str. 4074
ZP_00187174 390 pfg--atgaRLVTTaanrlr---eedgrFAVVTACAAGGLGHAMLVER 432 Rubrobacter xylanophilus DSM 9941
AAL39269    420 p-----faaTGVRLcmhtanrlvredgkLGVVAACAAGGQGVAMLIER 462 fruit fly
XP_420004   431 gatgcrlaiTAAHRlkke-------ggqYGLIAACAAGGQGHAMLVEL 471 chicken
AAH45222    427 gatgcrlvmAAAHRlkke-------ggqYGLVAACAAGGQGHGMIVEL 467 African clawed frog
AAH46041    428 gatgcrlvtTVAHRlqke-------ggqYGLVAACAAGGQGHAMVIEA 468 zebrafish
BAB20046    428 gatgcrlttTAANRlkae-------dkqFALLAACAAGGLGHSMIVER 468 Polyandrocarpa misakiensis

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