1QFL,1AFW,1M3K,1M1T


Conserved Protein Domain Family
thiolase

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cd00751: thiolase 
Click on image for an interactive view with Cn3D
Thiolase are ubiquitous enzymes that catalyze the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA, a 2-step reaction involving a covalent intermediate formed with a catalytic cysteine. They are found in prokaryotes and eukaryotes (cytosol, microbodies and mitochondria). There are 2 functional different classes: thiolase-I (3-ketoacyl-CoA thiolase) and thiolase-II (acetoacetyl-CoA thiolase). Thiolase-I can cleave longer fatty acid molecules and plays an important role in the beta-oxidative degradation of fatty acids. Thiolase-II has a high substrate specificity. Although it can cleave acetoacyl-CoA, its main function is the synthesis of acetoacyl-CoA from two molecules of acetyl-CoA, which gives it importance in several biosynthetic pathways.
Statistics
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PSSM-Id: 238383
View PSSM: cd00751
Aligned: 223 rows
Threshold Bit Score: 222.354
Threshold Setting Gi: 50955605
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitedimer interface
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1QFL; Zoogloea ramigera biosynthetic thiolase with bound reaction intermediate
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1QFL_A        3 VIASAARTAVGSFnGAFANTPAHELGATVISAVLEragvaagevNEVILGQVLPagegqNPARQAAMKAGVPqeATAWGM 82
gi 6136703   16 AIVSGLRTPFARQaTAFHGIPAVDLGKMVVGELLArseipaeviEQLVFGQVVQmpeapNIAREIVLGTGMNvhTDAYSV 95
gi 465684    27 VLVDAVRTPFVVSgTVFKDLMAVDLQKEAIKALVEktklpyeqlDHIICGTVIQecktsNIAREAALLAGVPdkIPAHTV 106
gi 32033527  15 AIVSGLRTPFARKdTGFKDAYATSLGTMVTNELLSrtaiehheiEQLVFGQVIQqpdipNPAREIALALNMPk-LQSYSI 93
gi 45547116  13 VLVDGVRTPFMRAgTAYLSQTSYDLARTVLRGLLEragvapesvGYVVMGTVIQnistsNVARDAALAAGLPssVPAHTV 92
gi 17861584  45 VLVDGVRTPFLTSgTTYSKLMPHELARHSLLSLLQknrldkeliDYIVYGSVIQevktsNIAREAALAAGFSnkTPAHTV 124
gi 50745166  55 VVVEGVRIPFLQSgTSYADLMPHDLARAALQGLLNrtsvprdvvDYIVYGTVIQevktsNVAREAALGAGFSdkTPAHTV 134
gi 28280033  51 VIVDGVRTPFLLSgTTYADLMPHDLARTALQGLLQrtnvprevvDYIVYGTVIQevktsNVAREAALGAGFSdkTPAHTV 130
gi 28374341  52 VLVEGVRTPFLLSgTTYSDLMPHDLARAALQGLLKrtgipkdavDYIVYGAVIQevktsNVAREAALGAGFSdkTPAHTV 131
gi 11995041  48 VLVEGVRTPFLMSgTQYKNLMPHDLAREALKGLLKrtemattdvDYIVLGTVIQesktpNIAREAALGAGFSerIPSHTV 127
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #                                                                            
1QFL_A       83 NQLXGSGLRAVALGMQQIATGdaSIIVAGGMESMSMAphcahlrggvkmgdfkm-----------------idtmikdgl 145
gi 6136703   96 SRACATSFQAVANVAESLMAGtiRAGIAGGADSSSVLpigvskklarvlvdvnkartmsqrlklfsrlrlrdlmpvppav 175
gi 465684   107 TLACISSNVAMTTGMGMLATGnaNAIIAGGVELLSDVpirynrnarkamlgmnkakdvpsklkiggqivknllspelpav 186
gi 32033527  94 SSSCLSGLQAIANVAGSIVSGsiSAGIAGGADSISNAplsisprviyklksifnaetle-------ekyrrfrhfswrdl 166
gi 45547116  93 TMACISANQAITSALETIRAGkaEAAVAGGVEVMSDTppqfgkkarerlflaqgyrspl-------efrrllsgmspsdf 165
gi 17861584 125 TMACISSNAAITTGMGLIATNtyDVIVAGGVEFMSDVpirhsrkmrslllkankaktlg-------qrlallstfrpdfl 197
gi 50745166 135 TMACISSNQAMTTGIGLIAAGqcDVVVAGGVELMSDVpirhsrkmrktlltlnraktlg-------qklaliskirpnyf 207
gi 28280033 131 TMACISSNQAMTTGVGLIASGqcDVIVAGGVEFMSDVpirhsrkmrktmlslnkaktlg-------qrlsvisrirpnyl 203
gi 28374341 132 TMACISSNQAMTTAVGLIAAGqcDAVVAGGVEFMSDVpirhsrkmrktmlslnkaktlg-------arlsllgsirlahl 204
gi 11995041 128 SQACISSNQAITTAMSLIASGqcQAVIAGGVDFMSDVpirfnrtmrklmldankaktmg------qrlglaskvifspmr 201
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1QFL_A      146 tdafygyHMGTTAENVAKQWql---------sRDEQDAFAVASQNKAEAAQKDGRFkDEIVPFIvkgrkgdiTVDADEYI 216
gi 6136703  176 aeystglRMGDTAEQMAKTYgi---------tREQQDALAHRSHQRAAQAWSDGKLkEEVMTAFipp--ykqPLVEDNNI 244
gi 465684   187 aefstgeTMGHSGDRLAAAFnv---------sRREQDEFAIRSHTLASEAAKNGKF-TDVVPVFldgk-kpkTIKEDNGI 255
gi 32033527 167 kphgvnlRDFTTQMSVAEVSeqmaq--dnhisRAEQDEYARLSNQRASDAWKIGLLkEEVMPSFprp--ykdFVVSDTLI 242
gi 45547116 166 lprapaiSEYSTGELMGESAdrlaa--afgvsREEQDEYALRTHTLAAKATESGRLaAEILPTAppp--dfeLLTEDNTI 241
gi 17861584 198 apelpavAEFSSGETMGHSAdrlas--afnvsRSEQDEYALRSHTLAKEAQEKGYF-TDLVPFKvsg--vdqIVDKDNGI 272
gi 50745166 208 apelpavAEFSTSETMGHSAdrlaa--afaisRVEQDEYALRSHTLAKKAQDEGLL-LDVVPFKvpg---kdTVTKDNGI 281
gi 28280033 204 apelpavAEFSTSESMGHSAdrlaa--afsvsRVEQDEYALRSHTLAKKAQDAGLL-SDIIPYKvpg---reTVEKDNGI 277
gi 28374341 205 apelpavAELSTAETMGHSAdrlaa--afgvsRLEQDEFALRSHTLAKKAQDGGLL-SDVVGFEvpg---kdIVSKDNGI 278
gi 11995041 202 ylspelpAIAEFSTNEVMGHsadrlaaafgitRQAQDEFAYRSHSLAHKATEEGKL-SDVLEFKvpg--vadLVKTDNGI 278
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1QFL_A      217 RhgATLDSMAKLRPAFDk-eGTVTAGNASGLNDGAAAALLMSEaeasrrgIQPLGRIVSWATVGVDPk-VMGTGPIPASR 294
gi 6136703  245 RgnSSLADYAKLRPAFDrkhGTVTAANSTPLTDGAAAVILMTEsrakelgLVPLGYLRSYAFTAIDVwqDMLLGPAWSTP 324
gi 465684   256 Rv-STLEKLSSLKPAFVkphGTVTAANASYLTDGASAALIMTEeyalangYKPKAYLRDYLYVAQDPkdQLLLSPAYVIP 334
gi 32033527 243 SaaTRASYYQRFSPIVDkeyATVTEANMPQPMDGAAAVLLMNEsrakalgLMPLGYIRSYAITGNDIwqDMFAGATIAAA 322
gi 45547116 242 RrdTSLEKLAALPPAFVkplGTVTAGNSSPLTDGAAATLLMEEdaaraagHEPKARLADYLYVAQDPgeELLLGPAYAVP 321
gi 17861584 273 Rv-SSPESLAKLRPAFVkpyGTVTAANASFLTDGASACIIMTEekakqlgLKPKAYLRDFLFVSQDPvnQLLLGPAYGIP 351
gi 50745166 282 Rp-SSLEQMSKLKPAFVkpyGTVTAANSSFLTDGASAMLLMSEekalalgYKPKAYLRDFVYVSQDPkdQLLLGPTYATP 360
gi 28280033 278 Rp-SSMEQMTKLKPAFIkphGTVTAANSSFLTDGASAVLIMSEekalamgYKPKAYLRDFVYVSQDPkdQLLLGPTYATP 356
gi 28374341 279 Rp-STMEQMAKLKPAFVkphGTVTAANSSFLTDGASAVLIMSEekalamgYKPKAYLRDFVYVSQDPkdQLLLGPTYATP 357
gi 11995041 279 Rp-TPLEKMAKLKAAFIkphGSITAANSSFLSDGGSACLIMEEskakamgLKPKAYLREFTYVSQDPkdQLLLGPAYATP 357
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                          #            
1QFL_A      295 KALERAGWKIGDLDLVEANEAFAAQACAVNKDLGWDp-----------------sIVNVNGGAIAIGHPIGASGARILn- 356
gi 6136703  325 LALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSErfarealgrahatgevddsKFNVLGGSIAYGHPFAATGARMIt- 403
gi 465684   335 KLLDKAGLTLKDVDVFEIHEAFAGQVLANLNAMDSDyfckeqmkrsgkfgrvpmdKLNLWGGSLSIGHPFGATGVRLAt- 413
gi 32033527 323 QALDRAELSLSDMDYIDIHESSASQILANIRFFESDdfak------------kqlNRTACLGKIDMDKLNHLGGSIAFgn 390
gi 45547116 322 RLLERNGLSLSDIDVLEIHEAFAGQVLAVLRALESDrfar------------erlGLGRRVGEVEMERVNAWGGSVSLgh 389
gi 17861584 352 KLLKKAGLTLKDIDSWEIHEAFAGQIVANLKALDSDwfck------------tylGLNEKVGTPDLSKWNNWGGSLSIgh 419
gi 50745166 361 KVLEKAGLTMADIDVFEFHEAFAGQILANMKAMDSDwfaqn----------ymgrKSKVGAPPLEKFNTWGGSLSLGHpf 430
gi 28280033 357 KVLERAGLTLADIDVFEFHEAFSGQILSNLKAMDSDwfaqn----------ymgrKAKVGAPALDKFNMWGGSLSLGHpf 426
gi 28374341 358 KVLEKSGLTMDDIDVFEFHEAFAGQIMANLKAMGSDwfaqt----------ymgrKTKVGVPPMEKFNTWGGSLSLGHpf 427
gi 11995041 358 IVLEKAGLKMSDIDVFEYHEAFAGQILANLAAMESEyfakn----------ymnrSTKVGAPPIEKFNLWGGSLSIGHpf 427
                       410       420       430       440
                ....*....|....*....|....*....|....*....|....*...
Feature 1                                         #             
1QFL_A      357 ---------TLLFEmkrr-------garKGLATLCIGGGMGVAMCIES 388
gi 6136703  404 ---------QTLHElrrr-------gggFGLVTACAAGGLGAAMVLEA 435
gi 465684   414 ---------HSAHRlkee-------kgqYAVIAACAAGGHGVGMLIEA 445
gi 32033527 391 p-----ravTSLRTviqslyalkrkgggISLVASGGLGGLGAAMVLES 433
gi 45547116 390 pfg--atgaRLVTTaanrlr---eedgrFAVVTACAAGGLGHAMLVER 432
gi 17861584 420 p-----faaTGVRLcmhtanrlvredgkLGVVAACAAGGQGVAMLIER 462
gi 50745166 431 gatgcrlaiTAAHRlkke-------ggqYGLIAACAAGGQGHAMLVEL 471
gi 28280033 427 gatgcrlvmAAAHRlkke-------ggqYGLVAACAAGGQGHGMIVEL 467
gi 28374341 428 gatgcrlvtTVAHRlqke-------ggqYGLVAACAAGGQGHAMVIEA 468
gi 11995041 428 gatgcrlttTAANRlkae-------dkqFALLAACAAGGLGHSMIVER 468

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