1THJ


Conserved Protein Domain Family
LbH_gamma_CA

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cd00710: LbH_gamma_CA 
Click on image for an interactive view with Cn3D
Gamma carbonic anhydrases (CA): Carbonic anhydrases are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism, involving the nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. There are three distinct groups of carbonic anhydrases - alpha, beta and gamma - which show no significant sequence identity or structural similarity. Gamma CAs are homotrimeric enzymes, with each subunit containing a left-handed parallel beta helix (LbH) structural domain.
Statistics
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PSSM-Id: 100039
View PSSM: cd00710
Aligned: 16 rows
Threshold Bit Score: 221.345
Threshold Setting Gi: 150401887
Created: 24-Dec-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitetrimer
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active gamma carbonic anhydrase is trimeric, with active sites located at the interface between two LbH subunits. The trimer structure contains three active sites and each subunit contributes to two active sites via residues located on two surfaces.
  • Comment:Carbonic anhydrases catalyze the reversible hydration of carbon dioxide through a two-step mechanism involving the nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site.
  • Structure:1THJ; Methanosarcina thermophila gamma carbonic anhydrase trimer binds three zinc molecules.
    View structure with Cn3D
  • Citation:PMID 8665839

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                       #                
1THJ_A        25 SAPVIDPTAYIDPEASVIGEVTIGANVMVSPMASIRSDEGM------PIFVGDRSNVQDGVVLHALetineegepiedni 98  Methanosarcina ...
15838686      34 DLPDVHESAFVDPTAILCGCVIVEAGVFIGPYAVIRADETTvegdikPIRIGIGANIQDGVVIHSKsg------------ 101 Xylella fastidi...
NP_841262      9 DQPIVHESAFVDPTAILCGRIVVHENVFIGPYAVIRADEVDetghmePITVGAHSNIQDGVVIHSKsg------------ 76  Nitrosomonas eu...
YP_518227     16 KHPKVHHTAYVHPTAVLIGDVRVCENVMICPNVVLRADEGL------TIIIGAGVNIQDGVIMHCLkg------------ 77  Desulfitobacter...
AAM04523       9 QHPRVSKRAWISETAVIIGNISIADYVFVGPNAVLRADEPGs-----SITVQSGCNVQDNVVVHSLsh------------ 71  Methanosarcina ...
6127222        8 HLPQIDQSAYIDQTAIICGKVIIHANVFVGPYAVIRADETDetgdmqPIIIGANSNIQDGVVIHSKag------------ 75  Acinetobacter s...
AAU21878      43 DIPDIDASSYIHHFASVIGSVVIGRNVFIGPFSSIRGDVGL------KIFISHDCNIQDGVVLHGLknyeynspvte-hs 115 Bacillus lichen...
YP_843114     15 SMPSVATTAYVDETAVVIGDVRIGERVYVGPCASIRADEAT------PIVISEECNVQDGAIFHGLkg------------ 76  Methanosaeta th...
YP_001325653  13 KMPKLGKNIYIDQNSVIIGDVIIEDGVYISPNAVIRCDEPPt----kGIIIKKNVNIQDGAVIHCLsg------------ 76  Methanococcus a...
YP_001324667  79 YPSISKTGTYIDPNAVVIGNVKIGDDVYVGPHALIRCDEIPt----eGIIIGNKVNIQDGVIIHALrg------------ 142 Methanococcus a...
Feature 1                           #    #                                                       
1THJ_A        99 vevdgkeyAVYIGNNVSLAHQSQVHGPAAVGDDTFIGMQAFVFKSKVGNNCVLEPRSAAIGVTIPDGRYIPagMVVTSqa 178 Methanosarcina ...
15838686     102 -------aAVTIGARTSIAHRAIVHGPCTIGERVFIGFNSVLFNCVVGDGCVVRHNAVVDGCDLPPGFYVPstERIGSst 174 Xylella fastidi...
NP_841262     77 -------aAVTIGERTSIAHRAIVHGPCTVGPDVFIGFNSVLFNCTIGEGCVVRHNAVVDGCDLPPGFYVPstQRIGPrt 149 Nitrosomonas eu...
YP_518227     78 -------gSIEIGQDCSIAHCALVHGPAVIGRETFVGFRAIVHNATLGKNSFISHGAMVLNVELPDDSSVPvgKIIQSea 150 Desulfitobacter...
AAM04523      72 -------sDVLVGKNTSLAHSCIVHGPCRIGEGCFIGFGAVVFDCNIGKDTLVLHRSVVRGIDIFSGRIVPdgTVITRqa 144 Methanosarcina ...
6127222       76 -------aSVIIGENSSIAHRSIIHGPCEIGHHVFVGFNSVLFNCKIGPNSAIRHNAVVDGRDLPANFYVPamSYIHPkt 148 Acinetobacter s...
AAU21878     116 vfkdresySIYIGEKVSLAPQCQIYGPVRIDKNVFVGMQSLVFDAYIQEDTVIEPGAKIIGVTIPPKRFVSagRVISNqe 195 Bacillus lichen...
YP_843114     77 -------sSIKLGKKVSVAHGAVVHGPMTIGDESFIGFNAVVHASTVGERCFIGHRALVMGVKLKDGSFVPhgSVIDTqd 149 Methanosaeta th...
YP_001325653  77 -------tGVIVGKDSSISHCTIIHGHAEIGDKSFIGFNSIVFNAEIGDNVVIGHNCVIDGTGDTIKIPNN--KWIPPns 147 Methanococcus a...
YP_001324667 143 -------tKIEIEDEASLAHGCIVHGPAKIDKNAFIAFGAVVFAAEIGQGALIGHNAVVDGIGVEGGLKIPagKLVPSgt 215 Methanococcus a...
Feature 1                                          
1THJ_A       179 eadkLPEVTDDYayshTNEAVVYVNVHLAEGYKE 212 Methanosarcina thermophila
15838686     175 dlgrIPRVTVAAs--eFSEDVAQTNNRLVEGYRR 206 Xylella fastidiosa 9a5c
NP_841262    150 dlstIPKVSPKAs--eFSEDVARTNNTLVQGYKH 181 Nitrosomonas europaea ATCC 19718
YP_518227    151 evreLPAIVVQEi--sFKHDVQQINRELGRGYRE 182 Desulfitobacterium hafniense Y51
AAM04523     145 yanaLEPITKEMt--eFKRSVVRANIELVEGYMK 176 Methanosarcina acetivorans C2A
6127222      149 dlsaYPPIDISIt--eFSESVITTNIELVQGYKA 180 Acinetobacter sp. ADP1
AAU21878     196 danrLPEITDSYpyhdLNSKMTSVNLELAKGYKK 229 Bacillus licheniformis ATCC 14580
YP_843114    150 kadaLGPVPDSLk--gFNAEVVEVNCEFAKGYRS 181 Methanosaeta thermophila PT
YP_001325653 148 siykDEDGILKAf--lSNGKIITNFTELPELPGW 179 Methanococcus aeolicus Nankai-3
YP_001324667 216 vvmkDIDGQVKAf--lPDGTVLTDLNDLPEVVDW 247 Methanococcus aeolicus Nankai-3

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