1BU8,1GPL,1RP1,1LPB,1HPL,1ETH


Conserved Protein Domain Family
Pancreat_lipase_like

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cd00707: Pancreat_lipase_like 
Click on image for an interactive view with Cn3D
Pancreatic lipase-like enzymes. Lipases are esterases that can hydrolyze long-chain acyl-triglycerides into di- and monoglycerides, glycerol, and free fatty acids at a water/lipid interface. A typical feature of lipases is "interfacial activation," the process of becoming active at the lipid/water interface, although several examples of lipases have been identified that do not undergo interfacial activation . The active site of a lipase contains a catalytic triad consisting of Ser - His - Asp/Glu, but unlike most serine proteases, the active site is buried inside the structure. A "lid" or "flap" covers the active site, making it inaccessible to solvent and substrates. The lid opens during the process of interfacial activation, allowing the lipid substrate access to the active site.
Statistics
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PSSM-Id: 238363
View PSSM: cd00707
Aligned: 51 rows
Threshold Bit Score: 257.942
Threshold Setting Gi: 21064559
Created: 24-Sep-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1BU8_A       35 IDTRFLLYT-----NENPNNYQKISat---epDTIKFSNFQldrKTRFIVHGFIDKgeDGWLLDMCKKMFqvekVNCICV 106
1HPL_A       35 VNTRFLLYT-----NENPDNFQEIVad----pSTIQSSNFNtgrKTRFIIHGFIDKgeESWLSTMCQNMFkvesVNCICV 105
gi 24582661 104 SSSSADFWId----ENNFEFPQRHKrd----tWQEMAEKFNpelDTKILVHGWKSStmSNSIQSIRGAYIergqVNVFAI 175
gi 24584740  75 PDVKYYIYT-----RHNPMDRQCLHideslekSNLTDSYFNpryPTKIIIHGYNSDmfLHPLQQMREEYLakadYNIIYV 149
gi 24656916  12 LCQFLGLHAalldsLLNQSTIYYLKpsa--dvSLENVEQLSsveSVKLIVHGYLGSctHGSIMPLRNAYTaqgyENVLVA 89
gi 55239697 574 VQFTGAWWYrs--cADRTLKQEMDVe------SFVPPPQLDlrrQLKVLIHGWNADrhHVSILPIRTAYLvqdaHNLLVA 645
gi 24581130  47 PKIQFYLYT-----RRTQEQPEFIDvl---dpNALYYTHFNprhPTKIIIHGFGGGrtLSPSPDLREAYFsvgeYNIIIV 118
gi 24581136   2 GYEVDCLAT-----FVDSTRENPILld----pLDLNPWNFQpprPLKILIHGYTGDrdFAPNSYIRPVLLdhedVYVISI 72
gi 48106114  21 QNVFLRLYN-----SNGSYIDENIRn------ASLFLPYIQknnLLIILIHGFTEDinSYDIKIMTNAYLentqHNIFLI 89
gi 24582804  23 TTAKFILYYgp-tvADSDIYDLTDFq------SLLEDEHLDlgkNTVLYLHGYLEDpdVESIHVIAEAYLerkdTNLIVL 95
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                        #                             #
1BU8_A      107 DWRRG-SRTEYTQASYNTRVVGAEIAFLVQVLStem-gySPENVHLIGHSLGAHVVGEAGRRLeg------hVGRITGLD 178
1HPL_A      106 DWKSG-SRTAYSQASQNVRIVGAEVAYLVGVLQssf-dySPSNVHIIGHSLGSHAAGEAGRRTng------aVGRITGLD 177
gi 24582661 176 NWKDQaDNIYYLTPARYTVQVGRAVAKLIDLLVeek-daDPNRIHLIGHSLGAHIMGYAGSYTky------rVNRITGLD 248
gi 24584740 150 DWSILsPGPCYISAVHNTKHAGTCTAQLVERLVe----tGNTDIHVIGFSLGAQVPNYIARNLss-----fmLPRITGLD 220
gi 24656916  90 DWGPV-ANLDYPSSRLAVKNVAQILAKLLEEFLqrh-giSLEGVHVIGHSLGAHIAGRIGRYFng------sLGRVTGLD 161
gi 55239697 646 DWSPA-AVLHYPTARDLVLPVGNHLGSILARFMkrl-giEPSQVHVIGHSLGAHIAGNVGRYLgg------kVRRVTALD 717
gi 24581130 119 DYADAvKEPCLSQMDWAPRFGSLCISQLVKYLArhprgvQPDDLHFIGYSVGAHIAGLVANYLkpe---egkLGRITALD 195
gi 24581136  73 DYGPLvRYPCYIQAVQNLPLVSRCLAQLINNLVdra-ivANDQIHLIGFSLGGQVAGQTANYVkr------kMKRITGLD 145
gi 48106114  90 DYRNV-SKESYIFAEQDINKIGKSMADALDDMIen--gvNFKKIHIIGISLGAQLAGIIGRNMny------kIGRITGLD 160
gi 24582804  96 DWGELaDGNYMFDAFPNLKQLGPELAKVLLKMFdh--glDIEKFHIVGHSMGGQLAGLLGREItkrtkgvrkIKRISALD 173
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1BU8_A      179 PAEPcfqgl-peevRLDPSDAMFVDVIHTDSApiipylgfGMSQKVGHLDFFPNGGKe-MPGCQknilstivdin----- 251
1HPL_A      178 PAEPcfqgt-pelvRLDPSDAQFVDVIHTDIApfipnlgfGMSQTAGHLDFFPNGGKe-MPGCQknvlsqivdid----- 250
gi 24582661 249 PARPafedcigpenHLDDTDANFVDVIHSCAGy------lGFRKPIGMVDFYPNGGGppQPGCKelsqifskfwelvsis 322
gi 24584740 221 PAMPlfits-gkadKLDPSDASYVDVIHTNALv------qGKMERCGHADFYMNGGIm-QPGCNgqki------------ 280
gi 24656916 162 PALPlfss--rsddSLHSNAAQFVDVIHTDYPl------fGDIRPRGTVDFYPNFGLapQPGCEnvdvvaas-------- 225
gi 55239697 718 PAGPlfal--dskdAVGPDTAQFVDVIHTDGMt------lGENIVRGHADFFPNGGTppQPGCEtldv------------ 777
gi 24581130 196 PTIFfyaga-nnsrDLDSTDAHFVDVLHTGAGi------lGQWHSSGHADFYVNGGTr-QPACVgsatl----------- 256
gi 24581136 146 PAKPlfilg-pdsrRLDKGDADFVDVIHTDVFg------rGYLRAAGHVDFYPNFGAk-QPGCMeenm------------ 205
gi 48106114 161 PVGPgyyi---lntHLSASDAEFVDIIHTDMGi------yGLALKIGHVDFFPNYGYrpQPGCPisen------------ 219
gi 24582804 174 PAFPlfy----pgtHLSANDAEFVDVIHTDAWl------yGAPTSTGTADFWPNGGYslQPGCPkrnykm---------- 233
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       #                                                               
1BU8_A      252 ---giwegtqnfvACNHLRSYKYYASSIl--nPDGFLGYPCssyek--fqqndCFPCpe---------egCPKMGHYADQ 315
1HPL_A      251 ---giwqgtrdfaACNHLRSYKYYTDSIl--nPDGFAGFSCasysd--ftankCFPCss---------egCPQMGHYADR 314
gi 24582661 323 ykqnweisfsfsaGCSHGRSYEYYAESIn--sPKGFYGVPCsglde--lkgknCTGG-------------KILMGDPVPR 385
gi 24584740 281 ----------nsfACSHQRAPAYFLESIr--sPKGFWGWACsgyis--yllgmCPPTn-----------fLLEAGENIRP 335
gi 24656916 226 ------kllheaySCSHNRAVMFYAESIg--mPENFPAVSCsltaiksrrvedCLREksktntenandyqTVFMGEHVNR 297
gi 55239697 778 ---------ftlhSCSHARSTGFFAESIp--lPNSFVACACsrdeis-rqadiCLPDvrdt-----ssreCVAMGEALEL 840
gi 24581130 257 ---------fqtlACDHTKVTPYFIESIt--tTRGFYAGPCpnlfs--yligwCEPKds----------eYVLMGEHCSH 313
gi 24581136 206 ---------qdpsSCNHERAPRFYAESIn--tTVGFWARQCsgwll--qlltlCPTTg-----------aQALLGYHVSD 261
gi 48106114 220 ---------ditrFCSHGRSIEFYVESIk---NSTAFIGKCefys-----lneCNIE-------------YIPMGYATPH 269
gi 24582804 234 --------lsdndLSSHRRSWWFWAESVsdryPIGFDAVPAkk--------wsDFKQnkiv-----encpPVVMGHHCPT 292
                       330
                ....*....|....*...
Feature 1                         
1BU8_A      316 fegktatveqtVYLNTGD 333
1HPL_A      315 fpgrtkgvgqlFYLNTGD 332
gi 24582661 386 ear------giFFVKTAN 397
gi 24584740 336 ttr------gmFMIDTND 347
gi 24656916 298 rswna-vnlgiSRWQQLA 314
gi 55239697 841 rsqra-arirpGSRPTQS 857
gi 24581130 314 kar------gnYYVTTNA 325
gi 24581136 262 elr------gsYFLQTAS 273
gi 48106114 270 nan------gnYYLITNS 281
gi 24582804 293 tih------gdFYLQTNG 304

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