1KPL,1KPK


Conserved Protein Domain Family
Voltage_gated_ClC

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cd00400: Voltage_gated_ClC 
Click on image for an interactive view with Cn3D
CLC voltage-gated chloride channel. The ClC chloride channels catalyse the selective flow of Cl- ions across cell membranes, thereby regulating electrical excitation in skeletal muscle and the flow of salt and water across epithelial barriers. This domain is found in the halogen ions (Cl-, Br- and I-) transport proteins of the ClC family. The ClC channels are found in all three kingdoms of life and perform a variety of functions including cellular excitability regulation, cell volume regulation, membrane potential stabilization, acidification of intracellular organelles, signal transduction, transepithelial transport in animals, and the extreme acid resistance response in eubacteria. They lack any structural or sequence similarity to other known ion channels and exhibit unique properties of ion permeation and gating. Unlike cation-selective ion channels, which form oligomers containing a single pore along the axis of symmetry, the ClC channels form two-pore homodimers with one pore per subunit without axial symmetry. Although lacking the typical voltage-sensor found in cation channels, all studied ClC channels are gated (opened and closed) by transmembrane voltage. The gating is conferred by the permeating ion itself, acting as the gating charge. In addition, eukaryotic and some prokaryotic ClC channels have two additional C-terminal CBS (cystathionine beta synthase) domains of putative regulatory function.
Statistics
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PSSM-Id: 238233
Aligned: 112 rows
Threshold Bit Score: 82.2266
Threshold Setting Gi: 46198537
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:Cl- selectivity filter
Evidence:
  • Comment:Mutations in these residues affect channel selectivity

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1KPL_A       43 GTLTGLVGVAFEKAVSWVQNMRigalvqv-------------adhafllWPLAFILSALLAMVGYFLvrk----faPEAG 105 Salmonella typhi...
ZP_00233470  16 GILVGVIAALFLAMVHFATTFLwdylpnq--------------fdfswyYPLLIGLIGSFFVGMVQLklg---dypRSMH 78  Listeria monocyt...
NP_897797    14 GAISGAGVALFMALIGLITRQLwgdpvvkg------------ldgqiplVWSIAICGGIGLVLSQLHrag----dhTLLP 77  Synechococcus sp...
ABF44961     26 GALVGGLCILLRLALGALLELGaqvtgyappgtpgeggllmafgtalpwGLLALPVIGAAYAWLVPGea------gEPLA 99  Deinococcus geot...
ABB26560     34 GAASGAGVALIISLIDLATRQLwggpveqa------------lgqapplAWSVLVCGGFGLLLSFIHrggs-stllPELP 100 Synechococcus sp...
AAN81378     20 GIASSLILIMVMKIASVLQNLLwqrlpgtl-----------giaqdsplWIIGVLTLTGIAVGLVIRfsq----ghAGPD 84  Escherichia coli...
NP_825833    30 GVAASLLFVGVSALAGKLQNVLwqdlpdal-----------gigrysvlWMIIMLTCTGVLVGLIVWkv-----pgHAGP 93  Streptomyces ave...
ABB34533     14 GALSGAGVAVVMGWIDGLSQLLwgdpvleg------------ldrglplGWSLLICGGSGLILSLLHr--------PGPN 73  Synechococcus sp...
YP_394965    17 SAIVGILLGLFYSLQSTLISLFwqk----------------------atFGSLLDAIMLMMVGLVIIisrhyfgplPQNL 74  Lactobacillus sa...
NP_964547    20 GILTALYLNLINYVIDFIWGYFnhhsnf----------------slrpaYPFLICISFGIIIGFLVKklg---sypLTIE 80  Lactobacillus jo...
Feature 1       #####                                    #####                                  
1KPL_A      106 GSGIPEIEGALeelrp-vrwwRVLPVKFIGGMGTLGAGmvLGREGPTVQIGGNLGRMVLdvfrm-rsaeaRHTLLATGAA 183 Salmonella typhi...
ZP_00233470  79 DNVAEAKKTGRme------yrKVLLPTILSAWIILTFGasVGPEAALIGIVGGATTWIIdhlkl--smahKEELVSLSVG 150 Listeria monocyt...
NP_897797    78 ELPETIADLRDpdh----appRDNGRAILGAALAQIGGgsIGPEALMTRLAALLSQRIWr---------dRDHELKFAAA 144 Synechococcus sp...
ABF44961    100 QLVRGYHARGQwp------gvVVQLRMLAATLLGYGAGllVGRDAPFTLLGQLATRLLGqatrl--dtveTRTLTLAGAA 171 Deinococcus geot...
ABB26560    101 ETMEDLRHPDRap-------kRDERRAILGAALAQIGGgsIGPEALMTRLATLISRRMWkg--------nDQALQSASVA 165 Synechococcus sp...
AAN81378     85 PACEPLIGAPIp--------pSALPGLIVALILGLAGGvsLGPEHPIITVNIALAVAIGarll---prvnRMEWTILASA 153 Escherichia coli...
NP_825833    94 DPATLGLGGAPpl------apAVLPGLLLATGLMLAGGpsLGPENPIIASNIALAYWLGrrfv---prlpGGLWIALAEA 164 Streptomyces ave...
ABB34533     74 TLLPELRDTLSdlrdpdhapkRDEVRGLLGAALAQIGGgcIGPEALMSRMAALISQRIWr---------gRDQKLQEATV 144 Synechococcus sp...
YP_394965    75 GVIKADLKQTGta------nyRYVLLQMLIPAVILVSGtsLGPEATLVSSTFLYSIWLAdkqryvaahfdDLRAQSIGMR 148 Lactobacillus sa...
NP_964547    81 EVLHDVRKSGKld-------yHSWWKSFTLGLLSLGAGgsIGPEASTTVLTSGMINWLG-----------DKIRLMTTHY 142 Lactobacillus jo...
Feature 1                                                                                       
1KPL_A      184 AGLSAAFn----------APLAGILFIIEEmrpqfr------ynlisIKAVFTGVIMSSIVFRIfngeapiiev----gk 243 Salmonella typhi...
ZP_00233470 151 AIISSIFh----------APFSGLAEEIDEssqakki----pknsklVLSLLISFSALGSFLWLksflkmpasifairlp 216 Listeria monocyt...
NP_897797   145 AGSLGLFg---------fPLLGGAVVQTHQrrdlia------rwipgALGGLAGFAAFHGIDQAsggslqrma------y 203 Synechococcus sp...
ABF44961    172 AGLGAVLh----------APLAAAVLIAEVlyrrfefe--fevlmpcVLAAVTGYAVYGAAFGFtplfsvemlp-----v 234 Deinococcus geot...
ABB26560    166 GSLGFLG-----------APLLGGMVVGERkqdvlr------rwipgILGGLSGFVAFHGINEAsggslknlpyv--wps 226 Synechococcus sp...
AAN81378    154 GTIGALFg----------TPVAAALIFSQTlngsnevp-lwdrlfapLMAAAAGALTTGLFFHPhfslpiah-------- 214 Escherichia coli...
NP_825833   165 ATIGALFg----------TPVAAALLLSEAlagqqakgplwdsvfapLVAAGAGAITTTLVAHPtldlglpp-------- 226 Streptomyces ave...
ABB34533    145 AGSLALFg----------APLLGGAVVGEAshpksrrqtfldrwlpgSLGGVAGFAAFHGIGAAsggslqrlp------y 208 Synechococcus sp...
YP_394965   149 LKVLATPhryllhyqqptAPLTKVATCQKKalsttyflngtawfsgvFILTGEPSLIIRLGQSHwqg------------- 215 Lactobacillus sa...
NP_964547   143 KSWVHVWqigidkdtllqSPKFSELFRTKNqkkwf--------itfnILIGLIGTILIFKLFPEegafgihe-------- 206 Lactobacillus jo...
Feature 1                                                                                       
1KPL_A      244 lsdapvntLWLYLILGIIFGVVGPVFNSLVLRTQDMfqrfhggeikkwVLMGGAIGGLCGILGliepaaagggfnl--ip 321 Salmonella typhi...
ZP_00233470 217 dmgwswwfILLFIPTIILGWLFSVYFQQLQVYIAKAasvi-----knkMVAAIIGGLSIGLFGiissfmlfsgehqliel 291 Listeria monocyt...
NP_897797   204 swpsnlgeDLGTLSSGALAGVVGWGLGWLLLHWRNWlerrq--llahwRWWPALTGLLLGITMhwlplvpfageeqlrpl 281 Synechococcus sp...
ABF44961    235 pslaqapaFLGVALAVTLAGWASLLACRVIPEGWTMg-----------YWRPLVGGAFGLLTAvlalwetpavlg---dg 300 Deinococcus geot...
ABB26560    227 nigesfdtLVAALLAGAVGCGLGLIFQRWRYWLEQRrlm------arwCWWPVVTGVLLGALMhwlplvpfggeeqmrpl 300 Synechococcus sp...
AAN81378    215 ygqmemtdILSGAIVAAIAIAAGMVAVWCLPRLHAMmhqm-----knpVFVLGIGGFILGILGviggpvslfkgldemqq 289 Escherichia coli...
NP_825833   227 larpgwtdVLAAMVVAAAAAALGMVAVYAFPYVHGAflrl----rhpmVALPAGGLVLGLLAAlgghltlfkgldevgdl 302 Streptomyces ave...
ABB34533    209 iwpsnlgeDLGTLSAGLLGGVVGCGLGWLLLRWQGWleqrk--llalwPWWPLLTGLLLGACMhwlplvpfagedqlrpl 286 Synechococcus sp...
YP_394965   216 ------rdLYWFLPLVLGGYLLGKLWLAGMVGLRKImqar----ltndQVRVLIGGLAIFLASlffphilfsgqhnfhlf 285 Lactobacillus sa...
NP_964547   207 rpihwswtILWYSLIPLIGGMLFAYFFLYLEKIFTRvetw----alpaLLKATLWGIVLSVLTlvtdyalfsgefhivpf 282 Lactobacillus jo...
Feature 1                                           #####                                       
1KPL_A      322 iaaagnfsvgLLLFIFITRVVTTLLCFSs---GAPGGIFAPMLALGTLLGTAFGMAaavlfpqyhleaGTFAIAGMGALM 398 Salmonella typhi...
ZP_00233470 292 tttvqnysipFLLIIALLKPVLVAICLAt---GWNGGAIFPAIFTSTVMGYIATYWidg-------stGFLITVFVTACC 361 Listeria monocyt...
NP_897797   282 legaggtgsfLFIISALVKLVLMGLCLEt---GWRGGIFFPLFLIACAVGVGLHDLwpgl-----gslGSWCGGITGAIY 353 Synechococcus sp...
ABF44961    301 agwvqlglagFLGPEALGEGAWRWLLLAlgarLALGGGVLPSVGVGGLLGAGLGAVlgv-------dpTVATLIGAVAFL 373 Deinococcus geot...
ABB26560    301 lenqqshpsaLLFLSAFGKLLMLGLCLEt---GWRGGIFFPVFLIACATGTGLHQIf----------pDLGSLGSWCGGL 367 Synechococcus sp...
AAN81378    290 mvanqafstsDYFLLAVIKLAALVVAAAs---GFRGGRIFPAVFVGVALGLMLHEHvp---------aVPAAITVSCAIL 357 Escherichia coli...
NP_825833   303 akdpdgwsagQFLTMAVVKLAALLVAASc---GFRGGRIFPSVFIGAAFGLFAHALvsa------vppALGVAAGVLGML 373 Streptomyces ave...
ABB34533    287 legqnsteawVLLLSAIVKLLMLGLCLEt---GWRGGVFFPLFLVACALGMGLHLLlpdl-----gslGSWCGALTGALY 358 Synechococcus sp...
YP_394965   286 ttiwqdqsmlFLMSRSLLKLILLTICLNt---GWLGGDIFPVLFASTAQGMAISQLfp--------qvDSLFVIGLIAIS 354 Lactobacillus sa...
NP_964547   283 sktalsysplFLLLIALIKTISTHVGFAm---GWRGGKIFPAIFASVAVGAAIAQFipi-------qpAITVSLAVVASI 352 Lactobacillus jo...
Feature 1                                                   
1KPL_A      399 AASv-----RAPLTGIVLVLEMTdnyqliLPMIITCLGATLLAQ 437 Salmonella typhimurium
ZP_00233470 362 TKIv-----GKPVLTASILLFIFp-----LQFFPFILLTAFIVN 395 Listeria monocytogenes str. 1/2a F6854
NP_897797   354 LTVl-----RSPLVALILGLGLLqgh-gaTGVVVGVAVAWLITH 391 Synechococcus sp. WH 8102
ABF44961    374 TVTl-----NAPVAAALLAIAWGg-----DALLPTALLTAGLAH 407 Deinococcus geothermalis DSM 11300
ABB26560    368 TGAfyrillPSPLVVVVLGLALLq-----GHGASGLLVGVAMAH 406 Synechococcus sp. CC9902
AAN81378    358 GIVlv--vtRDGWLSLFMAAVVVp----nTTLLPLLCIVMLPAW 395 Escherichia coli CFT073
NP_825833   374 LAIt-----RQGWVSLFTAAVLVa----sPAIIALLCIASLPAW 408 Streptomyces avermitilis MA-4680
ABB34533    359 RFVl-----PTPLAVLVLGIALLqgh-gtAGLLVGLAMAQLIRG 396 Synechococcus sp. CC9605
YP_394965   355 MGSai---lEAPLVAGLIMVILFl----pPNLFWVGIIATGIVL 391 Lactobacillus sakei subsp. sakei 23K
NP_964547   353 TIIl----eKPLLTAILLIFLLPis--laPLIFITAYIVMVIHK 390 Lactobacillus johnsonii NCC 533

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