Conserved Protein Domain Family
Ecotin

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cd00242: Ecotin 
Click on image for an interactive view with Cn3D
Protease Inhibitor Ecotin; homodimeric protease inhibitor
Protease Inhibitor Ecotin; homodimeric protease inhibitor which binds two chymotrypsin-like serine proteases to form a heterotetramer. Found in bacterial periplasm. Inhibits a broad range of serine proteases including collagenase, trypsin, chymotrypsin, elastase, and factor Xa but not thrombin. Inhibition mechanism involves binding at two different protease contact sites: the primary and secondary binding sites. Primary site loops of ecotin bind to the active site of target proteases in a substrate-like manner with the P1 residue in ecotin mimicking the interactions of a canonical P1 substrate residue.
Statistics
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PSSM-Id: 153074
View PSSM: cd00242
Aligned: 9 rows
Threshold Bit Score: 221.198
Threshold Setting Gi: 15387770
Created: 4-Sep-2001
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:primary substrate binding site
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1EZU_A        5 QPLEKIAPYPQAEKGMKRQVIQLTPQEDES---TLKVELLIGQTLEVDC----NLHRLGGKLENKTLEGWGFPYYVFDKV 77
1AZZ_C        5 QPLEKIAPYPQAEKGMKRQVIQLTPQEDES---TLKVELLIGQTLEVDC----NLHRLGGKLENKTLEGWGYDYYVFDKV 77
gi 13431479  20 AKLDEKVPYPKADAGFTRQVIHLPKQDAED---AFKVEIIAGKTLEADC----NQQRLGGELEEHTLEGWGYSYYRLDKV 92
gi 15387770   2 SYCKIEAPYPEAEAGEKRIIFALDPKGDEVeqeQCMLQLIPGRVLEMSRndaaNHQTLGGSIEQHTVEGFGAKFFHVKLA 81
gi 15387788  12 TLADFKAPYPEPTSQQRRYVIFLDPKGDSKelnDYKVELIPGRVEKVDGt---NVYRMGGNIEERTIDGWGYPYYIVTLT 88
gi 15802762  25 QPLEKIAPYPQAEKGMKRQVIQLTPQEDES---TLKVELLIGQTLEVDC----NLHRLGGKLESKTLEGWGYDYYVFDKV 97
gi 15979270  31 QPLEKIAPYPQAEKGMSRQVIFLEPQKDES---RFKVELLIGKTLNVDC----NRHMLGGNLETRTLSGWGFDYLVMDKI 103
gi 16761190  27 QPLEKIAPYPQAEKGMKRQVITLTPQQDES---TLKVELLIGQTLNVDC----NQHRLGGTLETKTLEGWGYDYYVFDNV 99
gi 16765590  27 QPLEKIAPYPQAEKGMKRQVITLTPQQDES---TLKVELLIGQTLNVDC----NQHRLGGTLETKTLEGWGYDYYVFDNV 99
                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1           ####                                                           
1EZU_A       78 SSPVSTMMACP--DGK-KEKKFVTAYLgdAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVV 141
1AZZ_C       78 SSPVSTMMACP--DGK-KEKKFVTAYLgdAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVV 141
gi 13431479  93 SGPMSTMMACP--GQK-KEQRFIPVVGe-GFLLCYNSKLPIVVYAPKDVEVRYRIWSASEKVEKAVS 155
gi 15387770  82 KQAVSTLMYVHaeDHGeKVRKFVAMSN--APMFPYRSRYPVVVYLPKDAELRYGIWCGGQQMQAATE 146
gi 15387788  89 TMSGTLMMPLG--DAAlKRPRFVAMNT--KNLYRYNSRLPIVVYMPKDGELRYRIWTVKSTGSGTAK 151
gi 15802762  98 SSPVSTMMACP--DGK-KEKKFVTAYLgdTGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVV 161
gi 15979270 104 SQPASTMMACPe-DSK-PQVKFVTANLgdAAMQRYNSRLPIVVYVPQGVEVKYRIWEAGEDIRSAQV 168
gi 16761190 100 TSPVSTMMACP--EGK-KEQKFVTAWLgeDGMLRYNSKLPIVVYTPANVDVKYRIWKADANVQNAIA 163
gi 16765590 100 TSPVSTMMACP--EGK-KEQKFVTAWLgeDGMLRYNSKLPIVVYTPANVDVKYRIWKADANVQNAVA 163

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