Conserved Protein Domain Family
G-alpha

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cd00066: G-alpha 
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Alpha subunit of G proteins (guanine nucleotide binding)
The alpha subunit of G proteins contains the guanine nucleotide binding site. The heterotrimeric GNP-binding proteins are signal transducers that communicate signals from many hormones, neurotransmitters, chemokines, and autocrine and paracrine factors. Extracellular signals are received by receptors, which activate the G proteins, which in turn route the signals to several distinct intracellular signaling pathways. The alpha subunit of G proteins is a weak GTPase. In the resting state, heterotrimeric G proteins are associated at the cytosolic face of the plasma membrane and the alpha subunit binds to GDP. Upon activation by a receptor GDP is replaced with GTP, and the G-alpha/GTP complex dissociates from the beta and gamma subunits. This results in activation of downstream signaling pathways, such as cAMP synthesis by adenylyl cyclase, which is terminated when GTP is hydrolized and the heterotrimers reconstitute.
Statistics
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PSSM-Id: 206639
View PSSM: cd00066
Aligned: 142 rows
Threshold Bit Score: 227.409
Threshold Setting Gi: 1169858
Created: 1-Nov-2000
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Display:
  next features
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1AZT: Bos taurus Gs-alpha binds GTP-gamma-S, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D
  • Comment:binding site for GTP and Mg2+ (bound by conserved Thr)
  • Citation:PMID 9242920

cd00066 is part of a hierarchy of related CD models.
Use the graphical representation to navigate this hierarchy.
cd00066 is a member of the superfamily cl21455.
cd00066:G-alphacd00154:Rabcd00157:Rhocd00876:Rascd00877:Rancd00878:Arf_Arlcd00879:Sar1cd00880:Era_likecd00881:GTP_translation_factorcd00882:Ras_like_GTPasecd01850:CDC_Septincd01851:GBPcd01852:AIG1cd01853:Toc34_likecd01860:Rab5_relatedcd01861:Rab6cd01862:Rab7cd01863:Rab18cd01864:Rab19cd01865:Rab3cd01866:Rab2cd01867:Rab8_Rab10_Rab13_likecd01868:Rab11_likecd01869:Rab1_Ypt1cd01870:RhoA_likecd01871:Rac1_likecd01873:RhoBTBcd01874:Cdc42cd01875:RhoGcd01876:YihA_EngBcd01878:HflXcd01879:FeoBcd01881:Obg_likecd01882:BMS1cd01883:EF1_alphacd01884:EF_Tucd01885:EF2cd01886:EF-Gcd01887:IF2_eIF5Bcd01888:eIF2_gammacd01889:SelB_eukcd01890:LepAcd01891:TypA_BipAcd01892:Miro2cd01893:Miro1cd01894:EngA1cd01895:EngA2cd01896:DRGcd01897:NOGcd01898:Obgcd01899:Ygr210cd01900:YchFcd04101:RabL4cd04102:RabL3cd04103:Centaurin_gammacd04104:p47_IIGP_likecd04105:SR_betacd04106:Rab23_likecd04107:Rab32_Rab38cd04108:Rab36_Rab34cd04109:Rab28cd04110:Rab35cd04111:Rab39cd04112:Rab26cd04113:Rab4cd04114:Rab30cd04115:Rab33B_Rab33Acd04116:Rab9cd04117:Rab15cd04118:Rab24cd04119:RJLcd04120:Rab12cd04121:Rab40cd04122:Rab14cd04123:Rab21cd04124:RabL2cd04126:Rab20cd04127:Rab27Acd04128:Spg1cd04129:Rho2cd04130:Wrch_1cd04131:Rndcd04132:Rho4_likecd04133:Rop_likecd04134:Rho3cd04135:Tc10cd04136:Rap_likecd04137:RheBcd04138:H_N_K_Ras_likecd04139:RalA_RalBcd04140:ARHI_likecd04141:Rit_Rin_Riccd04142:RRP22cd04143:Rhes_likecd04144:Ras2cd04145:M_R_Ras_likecd04146:RERG_RasL11_likecd04147:Ras_dvacd04148:RGKcd04149:Arf6cd04150:Arf1_5_likecd04151:Arl1cd04152:Arl4_Arl7cd04153:Arl5_Arl8cd04154:Arl2cd04155:Arl3cd04156:ARLTS1cd04157:Arl6cd04158:ARD1cd04159:Arl10_likecd04160:Arfrp1cd04161:Arl2l1_Arl13_likecd04162:Arl9_Arfrp2_likecd04163:Eracd04164:trmEcd04165:GTPBP1_likecd04166:CysN_ATPScd04167:Snu114pcd04168:TetM_likecd04169:RF3cd04170:EF-G_bactcd04171:SelBcd04172:Rnd3_RhoE_Rho8cd04173:Rnd2_Rho7cd04174:Rnd1_Rho6cd04175:Rap1cd04176:Rap2cd04177:RSR1cd08771:DLP_1cd09912:DLP_2cd09913:EHDcd09914:RocCORcd09915:Ragcd11383:YfjPcd11384:RagA_likecd11385:RagC_like1GOT A1TND C220275224538271358519118858763472287741KJY A1KJY C1Y3A D1CIP A1SVS A4911822345382315314188042321499631182496801428612613461021056781637590896244151082321364544559217507897450405147223707567572075675513517510719741598856607072472295991754093014133507175618321413350517562782175079033043241209914498284450287657503124017597000505528363002465824948791688163414794430314182585175135395493364170213450991264418071013793385053774902494120988116985819068016470282572354786680815366810930668272411AZT A1U0H C1CJT C1TL7 C1CUL C64714464032153752402723240143427258181748121971989417232143472078254902498107278832BCJ Q55715810475844425145853427258792162731822711867174812074721992247226407575246894721992321483610472264064902496504171224722560817481211174812151ZCB A24111250570330241ZCA B201476956165177242725770195283155337745566083172981411103774610644664505434884498107850307143171615502903456846828128327467496077175684073201682123214677270389203347323010241056607074668096651756183856607054949880710226056681068666814430668188551756183417561836668073831GOT A1TND C220275224538271358519118858763472287741KJY A1KJY C1Y3A D1CIP A1SVS A4911822345382315314188042321499631182496801428612613461021056781637590896244151082321364544559217507897450405147223707567572075675513517510719741598856607072472295991754093014133507175618321413350517562782175079033043241209914498284450287657503124017597000505528363002465824948791688163414794430314182585175135395493364170213450991264418071013793385053774902494120988116985819068016470282572354786680815366810930668272411AZT A1U0H C1CJT C1TL7 C1CUL C64714464032153752402723240143427258181748121971989417232143472078254902498107278832BCJ Q55715810475844425145853427258792162731822711867174812074721992247226407575246894721992321483610472264064902496504171224722560817481211174812151ZCB A24111250570330241ZCA B20147695616517724272577019528315533774556608317298141110377461064466450543488449810785030714317161550290345684682812832746749607717568407320168212321467727038920334732301024105660707466809665175618385660705494988071022605668106866681443066818855175618341756183666807383
cd00066 Sequence Cluster
cd00066 Sequence Cluster
Sub-family Hierarchy
 cd00066 Branch
 Whole Hierarchy
 [Download CDTree]
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #    ###                                                                  
1AZT_A       41 HRLLLLGAGESGKSTIVKQMRILHvnGFNGEggeedpqaarsnsdgEKATKVQDIKNNLKEAIETIVAAMSNlvppv-el 119
1ZCB_A       34 VKILLLGAGESGKSTFLKQMRIIHgqDFDQR---------------AREEFRPTIYSNVIKGMRVLVDAREKlhip---w 95
1ZCA_B       36 VKILLLGAGESGKSTFLKQMRIIHgrEFDQK---------------ALLEFRDTIFDNILKGSRVLVDARDKlgip---w 97
gi 24111250  49 VKILLLGAGESGKSTFLKQMRIIHgqDFDQR---------------AREEFRPTIYSNVIKGMRVLVDAREKlhip---w 110
gi 57033024  49 VKILLLGAGESGKSTFLKQMRIIH--GQDFDl-------------rAKEEFRSTIYSNVIKGIRVLVDAREKlhip---w 110
gi 42725770  39 VKVLLLGAGESGKSTFLKQMKIIHeqQFTDQ---------------EVKEFRNIIYGNIIKGMKVLADARDKlgip---w 100
gi 20147695  58 VKILLLGAGESGKSTFLKQMRIIHgrEFDQK---------------ALLEFRDTIFDNILKGSRVLVDARDKlgip---w 119
gi 61651772  62 VKILLLGAGESGKSTFLKQMRIIHgkEFDQK---------------ALLDFRDTIFENVIKGMRVLVDARDKlgis---w 123
gi 7496077   87 LKILLLGGPECGKSTIFKQMKIIHmnGFSDL---------------DYVNFRYLIYSNIMQSMDQLLEAAEFfh------ 145
gi 19528315 133 VKLLLLGAGESGKSTFLKQMRIIH--GVNFDy-------------eLLLEYQSVIYQNVIRGMQVLLDAREKlniawgsd 197
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AZT_A      120 anPENQFRVDYILSvmnv--------pdfdFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYV 191
1ZCB_A       96 gdNKNQLHGDKLMAfdtrapmaaqgmvetrVFLQYLPAIRALWEDSGIQNAYDRRREFQLGESVKYFLDNLDKLGVPDYI 175
1ZCA_B       98 qhSENEKHGMFLMAfenkag----lpvepaTFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYF 173
gi 24111250 111 gdNSNQQHGDKMMSfdtrapmaaqgmvetrVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYI 190
gi 57033024 111 geSANQKHGEVMMAfdtrsamvaqgmvetqVFMAHLVSIRALWADSGIQTAYDRRREFQLGESVKYFLDNLGKLGDKDYL 190
gi 42725770 101 gdSGNEKHAEFVMSfntqaa-----qleppLFVQYVQPCVELWKDSGIQSAFDRRREFQLADSVKYFLDEIDRVGRKDYI 175
gi 20147695 120 qySENEEHGMFLMAfenkag----lpvepaTFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYF 195
gi 61651772 124 qnSENEKHGMFVMSfenkag----mavepcTFQLYVPALQALWNDSGIQEAYGRRSEFQLSESVKYFLDNLDRIGQLSYV 199
gi 7496077  146 fpPDDSPSIRRALNhyksykvr-ystseveLNRELADSLSKLYNAEFIKSVLNRKNELKLLDSAVYFLDDIDRISAHEYK 224
gi 19528315 198 grEQDAYDAKLMECnsl----------dvpKFMEYAPPISRLWQDRGIRRAFERRREFQISDSVSYFLDEIQRLATPDYV 267
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #                  #  #                                             
1AZT_A      192 PSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFN-DVTAIIFVVASssynmvi---------redn 261
1ZCB_A      176 PSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFD-SVTSILFLVSSsefdqvl---------medr 245
1ZCA_B      174 PSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFD-GITSILFMVSSseydqvl---------medr 243
gi 24111250 191 PSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFD-SVTSILFLVSSsefdqvl---------medr 260
gi 57033024 191 PTQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFD-SVTSILFLVSSseydqvl---------medr 260
gi 42725770 176 PSLTDILHSRKATKAFQEHVIDIRNVPFRFVDVGGQRSQRQKWFQCFE-SVTSILFLASSsefdqvl---------medr 245
gi 20147695 196 PSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFD-GITSILFMVSSseydqvl---------medr 265
gi 61651772 200 PSRQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFD-GITSILFMVSSseydqvl---------medr 269
gi 7496077  225 PTEMDVLRARVPTTGITEIEFPFKQASLRMVDVGGQRSEQRKWIHCFD-NVNGVLFIAAIsgynlydedeenrkddgtpt 303
gi 19528315 268 PTHKDILHCRKATKGVYEFCVKVQNIPFVFVDVGGQRTQRQKWTRCFDsSVTSIIFLVSSsefdqvl---------aedr 338
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      ## #                                             
1AZT_A      262 qtNRLQEALNLFKSIWNnrwl-rtiSVILFLNKQDLLAEKVLAgksKIEDY---FPEFaryttpedatpepgedpRVTRA 337
1ZCB_A      246 qtNRLTESLNIFETIVNnrvf-snvSIILFLNKTDLLEEKVQVv--SIKDY---FLEFegd------------phCLRDV 307
1ZCA_B      244 rtNRLVESMNIFETIVNnklf-fnvSIILFLNKMDLLVEKVKSv--SIKKH---FPDFkgd------------phRLEDV 305
gi 24111250 261 ltNRLTESLNIFETIVNnrvf-snvSIILFLNKTDLLEEKVQIv--SIKDY---FLEFegd------------phCLRDV 322
gi 57033024 261 qtNRLTESLNIFETIVNnrvf-snvSIILFLNKTDLLEEKVKIv--SIKDY---FADFege------------phCLEDV 322
gi 42725770 246 itNRLLESCNIFDTIVNhkcf-asiSIILFLNKTDLLEEKIKHv--SIKDY---FPNFqgd------------phSMNDV 307
gi 20147695 266 rtNRLVESMNIFETIVNnklf-fnvSIILFLNKMDLLVEKVKTv--SIKKH---FPDFrgd------------phRLEDV 327
gi 61651772 270 rtNRLVESMNIFETIVNnklf-snvSIILFLNKMDLLVEKVRKv--SICKH---FSDFrgd------------phRLVDV 331
gi 7496077  304 ktNRLRYSMELFKRIANhqcfskktAMILFLNKIDIFKEKIGKy--PLTTC---FKNYkg-------------vnAFEPA 365
gi 19528315 339 ktNRLEESKNIFDTIVNnatf-kgiSIILFLNKTDLLEQKVCN---PETDIrwyYPHFngn------------phSVLDV 402
                       330       340       350       360       370
                ....*....|....*....|....*....|....*....|....*....|.
Feature 1                                  ##                      
1AZT_A      338 KYFIRDEFLRIStasgdgrHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHL 388
1ZCB_A      308 QKFLVECFRGKRrdq--qqRPLYHHFTTAINTENIRLVFRDVKDTILHDNL 356
1ZCA_B      306 QRYLVQCFDRKRrn---rsKPLFHHFTTAIDTENIRFVFHAVKDTILQENL 353
gi 24111250 323 QKFLVECFRNKRrdq--qqKPLYHHFTTAINTENIRLVFRDVKDTILHDNL 371
gi 57033024 323 QKFLVNCFRNKRrdq--qqKPLYHHFTTAINTENIRLVFRDVKDTILHDNL 371
gi 42725770 308 QNFILKMFDVRRrer--gsKALFHYFTTAVDTNNIRYVFQAVRDTILQENL 356
gi 20147695 328 QRYLVQCFDRKRrn---rsKPLFHHFTTAIDTENVRFVFHAVKDTILQENL 375
gi 61651772 332 QAYLVQCFNRKRrn---riKPLFHHFTTAIDTENIRFVFHAVKDTILQENL 379
gi 7496077  366 CKYVTDRFSRLVsgdiqheKPLYTHITNATDTRNIDRVFDSCMDVIFKISM 416
gi 19528315 403 QNFILQMFMSVRrss--siSRIYHHFTTAIDTRNINVVFNSVKDTILQRNL 451

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