1AZT,1CJT,1GOT,1KJY,1SVS,1CIP,1KJY,1Y3A,2BCJ,1TND,1ZCB,1ZCA,1CUL,1U0H,1TL7


Conserved Protein Domain Family
G-alpha

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cd00066: G-alpha 
Click on image for an interactive view with Cn3D
Alpha subunit of G proteins (guanine nucleotide binding)
The alpha subunit of G proteins contains the guanine nucleotide binding site. The heterotrimeric GNP-binding proteins are signal transducers that communicate signals from many hormones, neurotransmitters, chemokines, and autocrine and paracrine factors. Extracellular signals are received by receptors, which activate the G proteins, which in turn route the signals to several distinct intracellular signaling pathways. The alpha subunit of G proteins is a weak GTPase. In the resting state, heterotrimeric G proteins are associated at the cytosolic face of the plasma membrane and the alpha subunit binds to GDP. Upon activation by a receptor GDP is replaced with GTP, and the G-alpha/GTP complex dissociates from the beta and gamma subunits. This results in activation of downstream signaling pathways, such as cAMP synthesis by adenylyl cyclase, which is terminated when GTP is hydrolized and the heterotrimers reconstitute.
Statistics
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Aligned: 142 rows
Threshold Bit Score: 227.409
Threshold Setting Gi: 1169858
Created: 1-Nov-2000
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1AZT: Bos taurus Gs-alpha binds GTP-gamma-S, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D
  • Comment:binding site for GTP and Mg2+ (bound by conserved Thr)
  • Citation:PMID 9242920

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              #    ###                                                                  
1AZT_A        41 HRLLLLGAGESGKSTIVKQMRILHvnGFNGEggeedpqaarsnsdgEKATKVQDIKNNLKEAIETIVAAMSNlvppv-el 119 cattle
1ZCB_A        34 VKILLLGAGESGKSTFLKQMRIIHgqDFDQR---------------AREEFRPTIYSNVIKGMRVLVDAREKlhip---w 95  house mouse
1ZCA_B        36 VKILLLGAGESGKSTFLKQMRIIHgrEFDQK---------------ALLEFRDTIFDNILKGSRVLVDARDKlgip---w 97  house mouse
NP_006563     49 VKILLLGAGESGKSTFLKQMRIIHgqDFDQR---------------AREEFRPTIYSNVIKGMRVLVDAREKlhip---w 110 human
AAH88948      49 VKILLLGAGESGKSTFLKQMRIIH--GQDFDl-------------rAKEEFRSTIYSNVIKGIRVLVDAREKlhip---w 110 African clawed ...
AAS38578      39 VKVLLLGAGESGKSTFLKQMKIIHeqQFTDQ---------------EVKEFRNIIYGNIIKGMKVLADARDKlgip---w 100 purple urchin
AAM12615      58 VKILLLGAGESGKSTFLKQMRIIHgrEFDQK---------------ALLEFRDTIFDNILKGSRVLVDARDKlgip---w 119 human
NP_001013295  62 VKILLLGAGESGKSTFLKQMRIIHgkEFDQK---------------ALLDFRDTIFENVIKGMRVLVDARDKlgis---w 123 zebrafish
T33351        87 LKILLLGGPECGKSTIFKQMKIIHmnGFSDL---------------DYVNFRYLIYSNIMQSMDQLLEAAEFfh------ 145 nematode
AAL90272     133 VKLLLLGAGESGKSTFLKQMRIIH--GVNFDy-------------eLLLEYQSVIYQNVIRGMQVLLDAREKlniawgsd 197 fruit fly
Feature 1                                                                                        
1AZT_A       120 anPENQFRVDYILSvmnv--------pdfdFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYV 191 cattle
1ZCB_A        96 gdNKNQLHGDKLMAfdtrapmaaqgmvetrVFLQYLPAIRALWEDSGIQNAYDRRREFQLGESVKYFLDNLDKLGVPDYI 175 house mouse
1ZCA_B        98 qhSENEKHGMFLMAfenkag----lpvepaTFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYF 173 house mouse
NP_006563    111 gdNSNQQHGDKMMSfdtrapmaaqgmvetrVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYI 190 human
AAH88948     111 geSANQKHGEVMMAfdtrsamvaqgmvetqVFMAHLVSIRALWADSGIQTAYDRRREFQLGESVKYFLDNLGKLGDKDYL 190 African clawed ...
AAS38578     101 gdSGNEKHAEFVMSfntqaa-----qleppLFVQYVQPCVELWKDSGIQSAFDRRREFQLADSVKYFLDEIDRVGRKDYI 175 purple urchin
AAM12615     120 qySENEEHGMFLMAfenkag----lpvepaTFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYF 195 human
NP_001013295 124 qnSENEKHGMFVMSfenkag----mavepcTFQLYVPALQALWNDSGIQEAYGRRSEFQLSESVKYFLDNLDRIGQLSYV 199 zebrafish
T33351       146 fpPDDSPSIRRALNhyksykvr-ystseveLNRELADSLSKLYNAEFIKSVLNRKNELKLLDSAVYFLDDIDRISAHEYK 224 nematode
AAL90272     198 grEQDAYDAKLMECnsl----------dvpKFMEYAPPISRLWQDRGIRRAFERRREFQISDSVSYFLDEIQRLATPDYV 267 fruit fly
Feature 1                    #                  #  #                                             
1AZT_A       192 PSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFN-DVTAIIFVVASssynmvi---------redn 261 cattle
1ZCB_A       176 PSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFD-SVTSILFLVSSsefdqvl---------medr 245 house mouse
1ZCA_B       174 PSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFD-GITSILFMVSSseydqvl---------medr 243 house mouse
NP_006563    191 PSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFD-SVTSILFLVSSsefdqvl---------medr 260 human
AAH88948     191 PTQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFD-SVTSILFLVSSseydqvl---------medr 260 African clawed ...
AAS38578     176 PSLTDILHSRKATKAFQEHVIDIRNVPFRFVDVGGQRSQRQKWFQCFE-SVTSILFLASSsefdqvl---------medr 245 purple urchin
AAM12615     196 PSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFD-GITSILFMVSSseydqvl---------medr 265 human
NP_001013295 200 PSRQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFD-GITSILFMVSSseydqvl---------medr 269 zebrafish
T33351       225 PTEMDVLRARVPTTGITEIEFPFKQASLRMVDVGGQRSEQRKWIHCFD-NVNGVLFIAAIsgynlydedeenrkddgtpt 303 nematode
AAL90272     268 PTHKDILHCRKATKGVYEFCVKVQNIPFVFVDVGGQRTQRQKWTRCFDsSVTSIIFLVSSsefdqvl---------aedr 338 fruit fly
Feature 1                                       ## #                                             
1AZT_A       262 qtNRLQEALNLFKSIWNnrwl-rtiSVILFLNKQDLLAEKVLAgksKIEDY---FPEFaryttpedatpepgedpRVTRA 337 cattle
1ZCB_A       246 qtNRLTESLNIFETIVNnrvf-snvSIILFLNKTDLLEEKVQVv--SIKDY---FLEFegd------------phCLRDV 307 house mouse
1ZCA_B       244 rtNRLVESMNIFETIVNnklf-fnvSIILFLNKMDLLVEKVKSv--SIKKH---FPDFkgd------------phRLEDV 305 house mouse
NP_006563    261 ltNRLTESLNIFETIVNnrvf-snvSIILFLNKTDLLEEKVQIv--SIKDY---FLEFegd------------phCLRDV 322 human
AAH88948     261 qtNRLTESLNIFETIVNnrvf-snvSIILFLNKTDLLEEKVKIv--SIKDY---FADFege------------phCLEDV 322 African clawed ...
AAS38578     246 itNRLLESCNIFDTIVNhkcf-asiSIILFLNKTDLLEEKIKHv--SIKDY---FPNFqgd------------phSMNDV 307 purple urchin
AAM12615     266 rtNRLVESMNIFETIVNnklf-fnvSIILFLNKMDLLVEKVKTv--SIKKH---FPDFrgd------------phRLEDV 327 human
NP_001013295 270 rtNRLVESMNIFETIVNnklf-snvSIILFLNKMDLLVEKVRKv--SICKH---FSDFrgd------------phRLVDV 331 zebrafish
T33351       304 ktNRLRYSMELFKRIANhqcfskktAMILFLNKIDIFKEKIGKy--PLTTC---FKNYkg-------------vnAFEPA 365 nematode
AAL90272     339 ktNRLEESKNIFDTIVNnatf-kgiSIILFLNKTDLLEQKVCN---PETDIrwyYPHFngn------------phSVLDV 402 fruit fly
Feature 1                                   ##                      
1AZT_A       338 KYFIRDEFLRIStasgdgrHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHL 388 cattle
1ZCB_A       308 QKFLVECFRGKRrdq--qqRPLYHHFTTAINTENIRLVFRDVKDTILHDNL 356 house mouse
1ZCA_B       306 QRYLVQCFDRKRrn---rsKPLFHHFTTAIDTENIRFVFHAVKDTILQENL 353 house mouse
NP_006563    323 QKFLVECFRNKRrdq--qqKPLYHHFTTAINTENIRLVFRDVKDTILHDNL 371 human
AAH88948     323 QKFLVNCFRNKRrdq--qqKPLYHHFTTAINTENIRLVFRDVKDTILHDNL 371 African clawed frog
AAS38578     308 QNFILKMFDVRRrer--gsKALFHYFTTAVDTNNIRYVFQAVRDTILQENL 356 purple urchin
AAM12615     328 QRYLVQCFDRKRrn---rsKPLFHHFTTAIDTENVRFVFHAVKDTILQENL 375 human
NP_001013295 332 QAYLVQCFNRKRrn---riKPLFHHFTTAIDTENIRFVFHAVKDTILQENL 379 zebrafish
T33351       366 CKYVTDRFSRLVsgdiqheKPLYTHITNATDTRNIDRVFDSCMDVIFKISM 416 nematode
AAL90272     403 QNFILQMFMSVRrss--siSRIYHHFTTAIDTRNINVVFNSVKDTILQRNL 451 fruit fly

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