Conserved Protein Domain Family
CBM20_CGTase

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cd05807: CBM20_CGTase 
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CGTase, C-terminal CBM20 (carbohydrate-binding module, family 20) domain. CGTase, also known as cyclodextrin glycosyltransferase and cyclodextrin glucanotransferase, catalyzes the formation of various cyclodextrins (alpha-1,4-glucans) from starch. CGTase has, in addition to its C-terminal CBM20 domain, an N-terminal catalytic domain belonging to glycosyl hydrolase family 13 and an IPT domain of unknown function. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99882
View PSSM: cd05807
Aligned: 6 rows
Threshold Bit Score: 183.146
Threshold Setting Gi: 118073811
Created: 10-Sep-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Display:
 
starch-bindingstarch-binding
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:starch-binding site 1 [chemical binding site]
Evidence:
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Structure:1DTU_A; Bacillus circulans CGTase CBM20 domain starch binding site 1 binds maltopentaose inhibitor; defined at 4A contacts.
    View structure with Cn3D
  • Citation:PMID 8672460

cd05807 is part of a hierarchy of related CD models.
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cd05807 is a member of the superfamily cl15347.
cd05467:CBM20cd05806:CBM20_laforincd05807:CBM20_CGTasecd05808:CBM20_alpha_amylasecd05809:CBM20_beta_amylasecd05810:CBM20_alpha_MTHcd05811:CBM20_glucoamylasecd05813:CBM20_genethonin_1cd05814:CBM20_Prei4cd05815:CBM20_DPE2_repeat1cd05816:CBM20_DPE2_repeat2cd05817:CBM20_DSPcd05818:CBM20_water_dikinasecd05820:CBM20_novamyl1DTU A1A47 A1CYG A628160221246058221180738111DTU A1A47 A1CYG A62816022124605822118073811
cd05807 Sequence Cluster
cd05807 Sequence Cluster
Sub-family Hierarchy
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 cd05807 Branch
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CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                       #                                  #           ##
1DTU_A       585 DQVSVRFVVNNATTALGQNVYLTGSVSELGNWDPAKAIGPMYNQVVYQYPNWYYDVSVPAGKTIEFKFLKKQg-STVTWE 663
1CYG_A       578 DQVSVRFVVNNATTNLGQNIYIVGNVYELGNWDTSKAIGPMFNQVVYSYPTWYIDVSVPEGKTIEFKFIKKDsqGNVTWE 657
1A47_A       582 NQICVRFVVNNASTVYGENVYLTGNVAELGNWDTSKAIGPMFNQVVYQYPTWYYDVSVPAGTTIQFKFIKKNg-NTITWE 660
gi 124605822 613 DQVTVRFIIDNAETKLGENVFLVGNVHELGNWDPEQSVGKFFNQIVYQYPTWYYDVNVPANTDLEFKFIKIDqdNNVTWQ 692
gi 62816022  612 DQISARFVVNDATTDVGENVYVVGNVHELGDWDTDRAVGPFFNQVVHEYPNWYYDVNLPAGTDIEFKFVKIAsdGTVTWE 691
gi 118073811 604 PQKSVRFILENGYTQWGEAIYLVGNTPELGSWDPNKAIGPFYNQVVEQYPNWYQDVSVPTNTQLQFKFIKKNg-NQVIWE 682
                         90       100
                 ....*....|....*....|.
Feature 1           #                 
1DTU_A       664 GGSNHTFTAPSsGTATINVNW 684
1CYG_A       658 SGSNHVYTTPTnTTGKIIVDW 678
1A47_A       661 GGSNHTYTVPSsSTGTVIVNW 681
gi 124605822 693 SGANQTYSSPEsGTGIIRVDW 713
gi 62816022  692 SGSNRQYTTPTdSTGEYSGTW 712
gi 118073811 683 SGSNHQLSAGS-TDSSIRVNW 702

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