Conserved Protein Domain Family
GH32_ScrB-like

?
cd18623: GH32_ScrB-like 
glycoside hydrolase family 32 sucrose 6 phosphate hydrolase (sucrase)
Glycosyl hydrolase family GH32 subgroup contains sucrose-6-phosphate hydrolase (sucrase, EC:3.2.1.26) among others. The enzyme cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose. These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
Statistics
?
PSSM-Id: 350135
Aligned: 184 rows
Threshold Bit Score: 276.313
Created: 5-Dec-2017
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1: active site [active site], 3 residue positions
Conserved feature residue pattern:[DEN] [DEN] [DEN]Click to see conserved feature residue pattern help
Evidence:
  • Comment:Three active site residues of the beta-propeller architecture of glycoside hydrolase families GH32, GH43, GH62 and GH68.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1         #                                                                           
NP_843277  38 GLMNDPNGVSYYNDe---YHVFYQWYPFgp---iHGMKHWGHVKSKDLINWErmpvaiiPTESYEsHGAYSGSAIVKDDL 111 Bacillus anthracis...
KIH70749   44 GSMNSIAGFVHHEGl---YHIFYQWSPFa----dKRTRCLYHVTSDDLVYFEn-----rGIVKMLsDGAYSGSAFVMEEG 111 Salinicoccus roseus
CEA03443   36 GFLGAPTGFNYVNGi---YHLFYEWSPDsdltvtPDSKYIYHATSKDLVTFQnegvkvrPDSLYDaENIFGGSLSKVFNE 112 Jeotgalicoccus sp....
CAL28455   44 GAAGKPNGMIFYKGk---YYISHEWYPFys---kQGLSYGYQYESSDLVHFNtdgsvlkPDSQYDnYGIKGGSTFVYQNN 117 Staphylococcus car...
OFL46748   35 GILGAPCGFFYINGl---YHIFYEWEPAtn---eESATYLYHVTSEDLIHFNnegiklrPDDLYDkDNLYGGNLTLINNE 108 Nosocomiicoccus
SEW10466   46 GELGHPTVFIHHKHh---YHVFYQWFPLga---dKDIPYWYHVTSHDLSLFEnkgifikPDSLYDdLGAFSGDALSFDDS 119 Jeotgalicoccus sp....
SDK85238   43 GSINAPAAVFYDGIne-kYHLFYTWLPVl----gEEEQHWYHVTSKDLATFTnegvklkPDTLFDdKGLSAGSSVSIDGR 117 Salinicoccus sp. Z...
SOC41287   43 GALNAPAAIFYDAAgd-mYHLFYTWLPLl----dEDEQYWYHVTSKNLADFKnagvklkPDTLFDsGGLSAGSAVHINKE 117 Salinicoccus kekensis
SHM21681   42 FGALAAPAAAFYDDeqeiYHLFYTWLPLl----gGDEQYWYHVTSDDFADFNnagvkmkPDALFDsEGLSAGSVLQINGT 117 Salinicoccus alkal...
OHR53015   44 GSAGKPNGLLFDGKh---YLISHVWYPFti---nNGLSYSYAYQTPDLIQNPteqimlkPDGLFDdEGIKGGSAIEINQQ 117 Staphylococcus sp....
Feature 1                                                             #                       
NP_843277 112 LHLLYTGNIKnpd-dsRDAKQCMATMd---sQYTMTKysnnpvidiiPDGYTKHVRDPKVWKHn---DIYYMLLGAQRKN 184 Bacillus anthracis...
KIH70749  112 IHLYHTEVRSntvyqqYTPFTIQAGAlh--pGRTVPVik------gvPPEYRWLMKDPKVWHEd---GRHIMLLGAASPE 180 Salinicoccus roseus
CEA03443  113 ISYFYTGERLks--deLLTTQLGGYMn---tDGKVQKysspl-irnfPAGYTEYLRNPYVTVId---REVVMFLGTMNRE 183 Jeotgalicoccus sp....
CAL28455  118 LHFLYTGLGLsre-klLFPYQLLAFInnhhqANKYPKpvv----enpPENFLPSLQDPSVFEKn---GQYYALFGAQTEE 189 Staphylococcus car...
OFL46748  109 LTLFFTGEQQks--dgVHTYQRAGVId---tDFKIKKypapi-lrevEPGYKQYFRNPYVFTHd---GEINMMIGTMNDE 179 Nosocomiicoccus
SEW10466  120 LMVFYHGRWMra--gnRIESLLSGEMn---eREKFDKdttiv-diemDNFELTKIKDPALFQHk---QDLYFITGAMNAE 190 Jeotgalicoccus sp....
SDK85238  118 EHIFYIGRSLsq--qsFKYKQLIAHMd---eRFKLIKhtvp--liegYEDRFRQFKDSEVIFKn---GRYIILIGAESRS 187 Salinicoccus sp. Z...
SOC41287  118 NHLFYVGRSDvr---sRCTYKVLAAAmd--lDHKLKKhtvp--liegFEAYYRQMKDPAMVVSg---GRYYMLIGAETRD 187 Salinicoccus kekensis
SHM21681  118 NHIFYMGRSRar---gRYRYKLLAADmd--gDDKLNKhtv-----plIEGFEDLYKQSKDSAMvrirDKYYLLIGAESKD 187 Salinicoccus alkal...
OHR53015  118 THFIYSAKSKdav-ieAPFTQLHAVKn---hTGEIEKyeqpi-nfevPEGYIQNVQDPKVFEKd---GARFALLSAQTEK 189 Staphylococcus sp....
Feature 1                                      #                                              
NP_843277 185 kTGTLLLYKSKDLYNWNFqgeittnlkeFGFMWECPDYFQLsgKDVLLFSPQGiekdredfhniYNVVYAIGHFDIKnly 264 Bacillus anthracis...
KIH70749  181 dYGRLLVFAGETPSSFEYrgelktgldqFGFMWEAPDYFELdgHQVFVFCPQGldky----rnsYWNIYQSGYIIGEadc 256 Salinicoccus roseus
CEA03443  184 eFGRVVIYRGPSVEELRFdgeldtgykvFGHLWEQPEFFDLnmDSVLIFNARGldkf----dthFWNIYQSGYMIGDfer 259 Jeotgalicoccus sp....
CAL28455  190 mQGRILVYHADNPLNWQYvkpiktqltdFGNVWTSPDYFTLngYDILMFTAQH-----------EEGSTQAGFLLGHldf 258 Staphylococcus car...
OFL46748  180 eFGRIIMYRGKSLEDLKFlgevntgydmFGYFWESPELFTLddAEVLMFNPRGldkf----khhFWNIYQSGYVINDfey 255 Nosocomiicoccus
SEW10466  191 eYGRLAVFKADDEYIRYKgeltt-gfneFGYQWEFPDIIDIngKTLLSFCPKGidky----qnhYHNMFQAGYMIGEidf 265 Jeotgalicoccus sp....
SDK85238  188 eNGCLAVFEADNESDFEYkgimetgleeFGWLWEYPDYFSVdgRDILVFCPQGvdry----gnyFRNNFQAGYLVGDidf 263 Salinicoccus sp. Z...
SOC41287  188 gRGRIAVFGADESSTFNHmgdldtgfdeFGWLWEYPGHFTAdgRDVLVFSPQGvdry----gdfFRNNYQSGYMIGSidf 263 Salinicoccus kekensis
SHM21681  188 gRGRLAVYGADELSTFEHlgdlqtdfdeFGWLWEYPDHFTVdgRDVLVFCPQGidry----gdlFRNNFQAGYLTGLldf 263 Salinicoccus alkal...
OHR53015  190 gQGRLLAYHADDLNQWQLiapvhtnlshFGDFWHASDYFTMngYDILTFTAEN-----------TEHAFKAGYLMGHlhf 258 Staphylococcus sp....
Feature 1                                                                       
NP_843277 265 f----hidsYYEADKGFDFYAPQTLedstSRRLLFAWAGSSEitypsddYMWAHCLTLPRELTLED 326 Bacillus anthracis str. Ames
KIH70749  257 ealtldhgpFHELDHGFDFYAPKTAlngdGERLMIGWMGMKEtay-ptgEAWSHCLTLPRELSIEE 321 Salinicoccus roseus
CEA03443  260 rgnyfihddFHEFDGGFDFYRPATTvdedGNRVLLAWMAAEEsrypssaDRTVNAMTIPRVLTIDE 325 Jeotgalicoccus sp. 13MG44_air
CAL28455  259 nnfhmnhgdFKLLDEGFGFTSPQTFvdntGEIVLMGLLEAQGdiellnqADLAPCLSLPRTLSIEQ 324 Staphylococcus carnosus subsp. c...
OFL46748  256 gslfvnhddYREFDGGFDFYRPVTTldseGRRVLIAWMSAEEskypteiEGYKNALTIPRVLTVNG 321 Nosocomiicoccus
SEW10466  266 drlemihgpFFEFDNGFDFYAPRLTkspkGKTILIGWMGMKNgeyptdeDGWRHCLTIPRELTIVK 331 Jeotgalicoccus sp. A76(2010)
SDK85238  264 ddysmkhgeFKEFDAGFDFYAPKVFtdqhDRQVVIGNLSMPTttyptekFHYDNCLSIPRVLNAEG 329 Salinicoccus sp. ZXM223
SOC41287  264 edavmthgdFREFDAGFDFYAPRIFtdktGRRVMIGTLGMPTttypdekFHVRHTLSIPRILEAEG 329 Salinicoccus kekensis
SHM21681  264 enagmthgkFREFDHGFDFYAPQTFtdkaQRQVIIGTLGMPTtsypeekFHVRHCLSLPRVLTFTD 329 Salinicoccus alkaliphilus
OHR53015  259 hnfkmnhgdFKLWDDGFGFVFPQTFedaeGSRVLIGLLKTEGeyehlpeIAQVPCLSIPRQLTMRN 324 Staphylococcus sp. HMSC061G12

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap