3ZXK,3ZXJ


Conserved Protein Domain Family
GH43_FsAxh1-like

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cd09001: GH43_FsAxh1-like 
Click on image for an interactive view with Cn3D
Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase
This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
Statistics
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PSSM-Id: 350115
Aligned: 116 rows
Threshold Bit Score: 257.828
Created: 21-May-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitechemical
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 3 residue positions
Conserved feature residue pattern:[DEN] [DEN] [DEN]Click to see conserved feature residue pattern help
Evidence:
  • Comment:Three active site residues of the beta-propeller architecture of glycoside hydrolase families GH32, GH43, GH62 and GH68.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 #                                                                   
3ZXK_A      7 TFTNPVLWEDHPALEVFRVGSVFYYSSSTFAySPGAPVLKSYDLVHWTPVTHsvprlnfgsnydlpsgtpgaYVKGIWAS 86  Humicola insolens
EMB16513   50 TFKNPFLLADYNNLDVIRVGQDFYMISASHH-FMGMPVLHSRDMVNWTLIARisrklkine---ryntpgqaYQHGTWAP 125 Rhodopirellula eur...
EIC00376    2 TYTNPIVLCDYSDPDVIRVGDTYYMTASSFNfLPGLPVLASRDLVHWSLASYacreipf------arydevqNACGIWAP 75  Treponema saccharo...
AEJ20563   18 EYRNPILFADYSDPDVIRVGDRFFGTASSFVhTPGLPVLESDDLVHWHLVSYaiprlpl------yydgpvrHGDGVWAP 91  Treponema caldariu...
SJZ99224    1 MYKNPIILCDYSDPDVIRVGGNYFMVASSFNfTPGLPILFSKNLVNWKLINYaaenipl------eqynfpqNAKGIWAP 74  Treponema berlinense
SHM93605    4 TYENPILKGDFSDPDAIRVGADFYMISSSFTyFPGLPLLHSKDLVHWNIVNYiakelpf------eaynhtmHKKGIWAP 77  Anaerosporobacter ...
Q8A3V2     34 TYINPILNADYSDPDVIRVGDKYYMVNSDFH-YMGMPVLESDDMINWKIISQvyrrldfp-----dwdtngnYGGGSWAP 107 Bacteroides thetai...
OHB57483   14 KYKNPVLFADYSDPDVVCVNDDFYMVASSFNcMPGIPVLHSKDLVNWKIIGHvyerlpf------ekydkpaHGQGSWAP 87  Planctomycetes bac...
CDE26472    2 KYTNPILQFDFSDPDAIRVGNDFYMVASSFNyVPGIPLLHSKNLVEWELINYvvdilpk-------efdevlHGEGLWAP 74  Clostridium sp. CA...
BAC87941   22 FYRNPVLNSDYSDPDVIRVGGDFYMVCSEFH-YMGMPVLHSKDLVNWTIIGRvydslkhd----pkydnmegYAKGSWAP 96  Clostridium sterco...
Feature 1                                                                #                    
3ZXK_A     87 TLRYRrsNDRFYWYGCv-eGRTYLWTSPggnalanngevppsaWNWQHTATIdn--cYYDAGLLIDDDd----------T 153 Humicola insolens
EMB16513  126 AIRFY--EGKFFVYVCtpqEGLLLSTATnp------------aGPWSPWQLIrneknWEDPCPLWDDVkdaggdgpdgrQ 191 Rhodopirellula eur...
EIC00376   76 SIRFH--GGKFYIFVAtpdEGIFFTSAEnp------------fGEWSALECVwkgkgFEDPCPIWDDDg----------R 131 Treponema saccharo...
AEJ20563   92 SLRYH--DGWFWIFFSmpdEGIFMTRAKhp------------hGPWSPIHCIhtvqgWIDPCPFWDDDg----------T 147 Treponema caldariu...
SJZ99224   75 SFSYH--KGIFYIIVGlpdEGIFLTQTDdi------------fGKWSPLKLIykakgFIDPALFFDDDg----------N 130 Treponema berlinense
SHM93605   78 SIRYY--NGEYYVYVCtpdEGLFLYKTKdp------------fGEWSSYHVLri-tgWIDPCPVWNEEg----------N 132 Anaerosporobacter ...
Q8A3V2    108 SIRHH--DGKFWIYFCtprEGLMMSTATdp------------hGPWSPLHCVkriggWEDPCPIWDDNg----------Q 163 Bacteroides thetai...
OHB57483   88 SIRYH--NGLFYVYFCtpnEGLFMANAKkp------------aGPWRLHHVTev-elWEDPCPIWDDDg----------N 142 Planctomycetes bac...
CDE26472   75 SIRYH--DSWFYCIIPdpdRGIYITKTQdp------------yGKWEPMWCViegkgIIDPCPIWKDD-----------K 129 Clostridium sp. CA...
BAC87941   97 AIRYH--NGRFYVYFCtpdEGLFMSTATdp------------aGPWSPLHEVvrvagWEDPCPFWDDDg----------N 152 Clostridium sterco...
Feature 1                                                      #                              
3ZXK_A    154 MYIAYGnp--------tINVAQLSPDgtrqvrvqQRVYAhpqg---qtvEGARMYKIRGNYYILVTrp---aDAEYVLRS 219 Humicola insolens
EMB16513  192 AYLIRSrlg-----agpLIVHKMSWDgtkv-agkGKIVArgp-----vlEGPKFHKRNGEYYIFSPeggidkGYQVVLRS 260 Rhodopirellula eur...
EIC00376  132 VFLVHAyvksrigfnskLGLLELDPVtlla-isdDKVIFdgtkt-qptiEGPKFYKRGGFYYIFAPaggvtdGWQAVLRS 209 Treponema saccharo...
AEJ20563  148 AYLVHAfaksrcgikskLQLFQMKEDasal-lgdGVVIFdgtqt-qptiEGPKLYKRNGYYYIFAPaggvkpGWQTVLRA 225 Treponema caldariu...
SJZ99224  131 AYVYHAyaksrcgfnskIGVLRFNKKfkvc-egeDKIIFdgtek-hptmEGPKVYKRNGFYYIFAPaggvteGWQTVLRS 208 Treponema berlinense
SHM93605  133 AYLVHAfagsraginsmLFIHRMNNEgtki-idnGRLVFdgrdn-hhttEGPKAYRKNGYYYMMAPaggvpsGWQLVMRS 210 Anaerosporobacter ...
Q8A3V2    164 AYLGRSqlg-----agpIILHKMSADgrtl-eddGHVIYtgp-----vaEGTKFHKRDGYYYISIPeggvgeGWQTILRS 232 Bacteroides thetai...
OHB57483  143 AYLVRSklr-----adiLYLHRMSKDgkkl-ldnGTIIFqdekt-qptiEGPKFLKKDGYYYIFAPaggvpeGWQTVLRS 215 Planctomycetes bac...
CDE26472  130 CYMAVGfaksrigfnssIGLYEVSPDlkkq-isaSYQIIydghdnnptiEGPKFNYRNGYFYIMCPagsvkgGWQVALRS 208 Clostridium sp. CA...
BAC87941  153 AYLGHStvg-----agpIIIHKMSPDgtk--lldDGVIVyvg----kiaEGTKIYKRNGYYYLIIPeggvktGWQTVLRS 221 Clostridium sterco...
Feature 1                                                                                  
3ZXK_A    220 TtgspfGPYEARTLVsriqgplanaGFAHQGGIVDAp-----DGTWHYVAFMDaYPGGRIPVVAPLRWTaDGWPEVV 291 Humicola insolens
EMB16513  261 Rni--wGPYEKRKTLeqgs---tniNGPHQGSWIDLd-----SGESWFYHFQQhMAWGRIGHLQPAGWDeNGWPWMG 327 Rhodopirellula europa...
EIC00376  210 Rki--dGKWEERIVLaqgs---ssvNGPHQGAWVESaeengvGIRDWFVHFQErGVFGRIVHLQPMKWTeDGWPLMG 281 Treponema saccharophi...
AEJ20563  226 Ksi--wGPYEERIVLhqgd---teiNGPHQGAYVALe-----DGTGWFIHFQDrDAYGRIWHLQPVQWI-NDWPLIG 291 Treponema caldarium D...
SJZ99224  209 Knp--eGPWEDKIVLkege---sgiNGPHQGAFVETp-----FGENWFIHFQDrGIFGRIVHLEPVLWK-NDWPLMG 274 Treponema berlinense
SHM93605  211 Khi--yGPYEVKITLhqgd---sviNGPHQGAYIEMe-----DGNDWFVHFQDrGAYGRITHLQPVRWE-EDWPLMG 276 Anaerosporobacter mob...
Q8A3V2    233 Kni--yGPYEKKVVLekgs---tnvNGPHQGALVDTp-----EGEWWFYHFQLtEPLGRVVHLQPAHWK-DGWPVIG 298 Bacteroides thetaiota...
OHB57483  216 Kni--yGPYEDKIVLhkgs---tniNGPHQGGIVQLk-----SGQWWFLHFQDrDAYGRIVHLQPVQWK-DGWPLIG 281 Planctomycetes bacter...
CDE26472  209 Kni--yGPYESKVVLmqng---tkiNGPHQGALIDLd----dNDNWAFIHFQDmWAYGRIVHLEPVMWY-NDWPICG 275 Clostridium sp. CAG:307
BAC87941  222 Ksi--yGPYERKVVLqtgn---tniNGPHQGALVELe-----NGESWFMHFQDtGILGRVCHLQPVTWV-DNWPLMG 287 Clostridium stercorarium

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