1ST8,1UYP,1W2T,2AC1,4EQV,3SC7,5ANN,5XHA,3LDR


Conserved Protein Domain Family
GH32_FFase

?
cd08996: GH32_FFase 
Click on image for an interactive view with Cn3D
Glycosyl hydrolase family 32, beta-fructosidases
Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
Statistics
?
PSSM-Id: 350110
Aligned: 232 rows
Threshold Bit Score: 124.288
Created: 24-May-2010
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 3 residue positions
Conserved feature residue pattern:[DEN] [DEN] [DEN]Click to see conserved feature residue pattern help
Evidence:
  • Comment:Three active site residues of the beta-propeller architecture of glycoside hydrolase families GH32, GH43, GH62 and GH68.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1          #                                                                           
1ST8_A      18 NWMNDPNGPMLYqg--vYHFFYQYNPyaatf----gdviIWGHAVSYDLVNWIhldpaiyp-----tqeadsksCWSGSA 86   chicory
5ANN_A      76 GWMNDPMGLYQRad-gsIHAGYQSHPkhiq-----wgniSQGAAYSSDFTSWTdfngsegyktiwpsqiydirgVFDGSI 149  Xanthophyllomyce...
5XHA_A      37 GQIGDPCLHYTDpatglFHVGFLHDGs------------GISSATTDDLATYQdlnqgnqviv--pggindpvaVFDGSV 102  Aspergillus kawa...
3LDR_A      37 GQIGDPCAHYTDpstglFHVGFLHDGd------------GIAGATTANLATYTdtsdngsfli-qpggkndpvaVFDGAV 103  Aspergillus japo...
SES49038    22 GWINDPHGVTWYeg--rYHVFFQHVPdtptw----athcHWGHMSGSDLVNWNddtaialk------pddgeagCWSGTL 89   Lentzea flaviver...
KJL38647    12 GWINDPHGIRHHgg--rYQVFFQQVPgrtdw----ardvHWGHAVGDDLLSLRqlpiais-------pgegdggIWTGSL 78   Microbacterium k...
OCL36594    10 GWLNDPHGVIHRad--gYHVFHQHAPetldw----glgiEWGHAIGDDLFSLRyagpaiq-------pgdgddgIWTGCI 76   Tessaracoccus
EIE26493    74 GWINDPNGPFYHng--rYHLFYQHIIngcew----dfgiVWGHAVTTDLVHWEhlppalmpt----pggldadgCFSGCC 143  Coccomyxa subell...
EDP05357     5 GWINDPNGPLFYkg--yYHMFYQYVEtgcqw----swglMWGHAVSRDLVTWEhlppaivps----fggfdgdgCFSGCA 74   Chlamydomonas re...
KFM23987    30 GWLNDPNGPVFYkg--rYHMFYQHLPeacewdfgivsfrVWGHSVSEDLIHWRqlppalvpt----pggldadgCFSGCC 103  Auxenochlorella ...
Feature 1                                                                                      
1ST8_A      87 TILpg-nIPAMLYTgsds------------------ksrQVQDLAWPknls---dpflREWVKHpknplitp----pegv 140  chicory
5ANN_A     150 IKEgidgYPTILYTstsfgplga--------tlneaegtETQSLAYTtd-------dgASWIKLgygagqnpviyewpet 214  Xanthophyllomyce...
5XHA_A     103 IPNgingLPTLLYTsvsylpihw--------sipytrgsETQSLAVSsd-------ggSNFTKLdqgpvipgp---pfay 164  Aspergillus kawa...
3LDR_A     104 IPVgvnnTPTLLYTsvsflpihw--------sipytrgsETQSLAVArd-------ggRRFDKLdqgpviadh---pfav 165  Aspergillus japo...
SES49038    90 VLDd--dRPTILYTsvgsv----------------drndSRIVMALGdn--------eMRNWEKlptpvle------app 137  Lentzea flaviver...
KJL38647    79 VVDda-gDARIFYTstdl------------------ptlDHGRIRTAtpr-----dddWIAWRKgdvvaet-------pa 127  Microbacterium k...
OCL36594    77 VEDd--lGPLAYYTsarq------------------pvlGLASVRTArpr-----dtaLTSWDKgdvvvtap-----agl 126  Tessaracoccus
EIE26493   144 IIDtd-gTPTILYTgvrlrsnpdcgplpppehdlnlpfvESQCFAVPetgydgepdplLANWVKseepflayp---ppds 219  Coccomyxa subell...
EDP05357    75 TLDen-gVPTILYTgvrlrssevcgplpppecdlgtaciETQCIAYAdpa-----dpkLTYWTKeevpflalp---ppnm 145  Chlamydomonas re...
KFM23987   104 VLDpasgLPALLYTgvrlrsnaasgpppppehdlglvwiESQIVAVPagpa----ddlLVEWVKherpfldlp---pnhv 176  Auxenochlorella ...
Feature 1            #                                                                         
1ST8_A     141 kddcFRDPSTAWLgp----------------------------dGVWRIVVGgdrd-nngmAFLYQSTDFvnwkrydqp- 190  chicory
5ANN_A     215 nltgFRDPYVFQSprleallan--------------ttsitnatGDHFATISggvhgdgarLFLYRQHTTgefikwtylg 280  Xanthophyllomyce...
5XHA_A     165 nvtaFRDPYVFQNptldsl--------------------lhsknNTWYTVISgglhekgpaQFLYRQYDSdfqyweylgq 224  Aspergillus kawa...
3LDR_A     166 dvtaFRAPFVFRSarldvllsldeevarnetavqqavdgwteknAPWYVAVSggvhgvgpaQFLYRQNGGnasefqywey 245  Aspergillus japo...
SES49038   138 gvteFRDPSVHRDg------------------------------AGWRMVVGcryq-gaatALMFRSLDLrqwseptilc 186  Lentzea flaviver...
KJL38647   128 gmsvFRDPWVFRDg------------------------------SGWSMVVGgrhddgaaaIVSYRSDDLhtwsygglal 177  Microbacterium k...
OCL36594   127 girdFRDPFVLRDg------------------------------DVWRMMVGsdsasghamMHGFVSTDGeswaydgval 176  Tessaracoccus
EIE26493   220 nlvgWRDPFIFEFkgkd------------------------grfKEWGMLMGsgikgkggsVMIYRSDNLrsgwrydgml 275  Coccomyxa subell...
EDP05357   146 qltaWRDPFVIGQpgq-------------------------dgqDCWTIMIGagvrdrggtALVYRSPTLrggswtyage 200  Chlamydomonas re...
KFM23987   177 qltgWRDPFIYTAntagvp-------------------gtgfrvQEHRMLIGsgikgqggtALVYSSPNIhgdwrfvgel 237  Auxenochlorella ...
Feature 1                                 #                                                    
1ST8_A     191 -----------------lssadatgTWECPDFYPVplnstngldtsvyggsvRHVMKAGf--------------EGHDWY 239  chicory
5ANN_A     281 plvtt-----gykesygewsgnygiNFETAGVTRLnpagaawd--ngsdttaVDFVTFGteqgr------adhqNHWPLW 347  Xanthophyllomyce...
5XHA_A     225 wwhept----nstwgngtwagrwafNFETGNVFSLdeygy--------nphgQIFTTIGtegsdlp-vvpqltsIHDMLW 291  Aspergillus kawa...
3LDR_A     246 lgewwqeatnsswgdegtwagrwgfNFETGNVLFLteegh-------dpqtgEVFVTLGtegsglp-ivpqvssIHDMLW 317  Aspergillus japo...
SES49038   187 qr-----------ksdpadplftgdGWECVQFIEId---------------gVWVLIACawtnk-----pddrmVYAVGD 235  Lentzea flaviver...
KJL38647   178 rp-----------aedpdtalarntLWECPQLFEId---------------gRHVLVLSvedpg-----epryvGYAVGR 226  Microbacterium k...
OCL36594   177 sr-----------hrdetdgvwmgaLWECPQVIEVd---------------gSWVMLTSvweag-----rlhyaGYAVGE 225  Tessaracoccus
EIE26493   276 ---------------ceadsvdtgaMWECPLLLALtvvp---------eehrRRSALGSl--------------HSLSNS 317  Coccomyxa subell...
EDP05357   201 ---------------lcrgagdtgvIWECPVMMHPppl------------paVTRLLASpaappmpkpmssppfICAIGG 253  Chlamydomonas re...
KFM23987   238 ---------------ctgssldtgfVWECPLLEGEe---------------aRTPRADGs--------------LSRAGS 273  Auxenochlorella ...
Feature 1                                                                                      
1ST8_A     240 TIGtyspdrenflpqnglsltgstldlrydygQFYASKSFfdd-----------aknRRVLWAWVPetdsqa-------- 300  chicory
5ANN_A     348 AAVdyevrdngsiea------viaysgvqdwgRSYAYASFpve------------gyRQVSVGWIYedddnvi------- 402  Xanthophyllomyce...
5XHA_A     292 VSGtvsrngsvsfn-------pnmagfldwgfSSYAAAGKvlpstslp-stksgapdRFISYVWLSgdlfeqae-----g 358  Aspergillus kawa...
3LDR_A     318 AAGevgvgseqegakve--fspsmagfldwgfSAYAAAGKvlpassavsktsgvevdRYVSFVWLTgdqyeqad-----g 390  Aspergillus japo...
SES49038   236 FDGavfeprsw--------------hrldhggTLYATTTFrda------------dgRWCALSWIRelepp--------- 280  Lentzea flaviver...
KJL38647   227 WSDgrfdaeaw--------------grlsfsrSPYAPTFFpda------------vgRPSLVFWLSeta----------- 269  Microbacterium k...
OCL36594   226 FAGgrftpra---------------wgrltygTYYAPTVFrda------------agRPCMMAWLRevr----------- 267  Tessaracoccus
EIE26493   318 YNNisafhpssngsh------pasptadrksaAAAGPQVQagfspr------kdlaaAGLRAGWEEdgkeppp------- 378  Coccomyxa subell...
EDP05357   254 GGGgnmgs-------------------apkqeGLEAAEDRgt-------------eqPAIVLSPTAavispprpp----p 297  Chlamydomonas re...
KFM23987   274 FQFsadggsharl----------ldldhaptlEFWRSPSQrg--------------sEAALLGAEGvavgarrarrlsld 329  Auxenochlorella ...
Feature 1                          
1ST8_A     301 -ddiekgWAGLQSFPRALWI 319  chicory
5ANN_A     403 -lakqfgYQGAFTLFRDLFV 421  Xanthophyllomyces dendrorhous
5XHA_A     359 fptnqqnWTGTLLLPRELRV 378  Aspergillus kawachii IFO 4308
3LDR_A     391 fptaqqgWTGSLLLPRELKV 410  Aspergillus japonicus
SES49038   281 --rsgrvRAGALSLPHRLSV 298  Lentzea flaviverrucosa
KJL38647   270 ----gedWAGTLSIPYLLSL 285  Microbacterium ketosireducens
OCL36594   268 ----gedWTGCLSLPYVLRV 283  Tessaracoccus
EIE26493   379 gggadgeGAGKSLLSEQLLN 398  Coccomyxa subellipsoidea C-169
EDP05357   298 helqqgkVAGSPAAPHAHIQ 317  Chlamydomonas reinhardtii
KFM23987   330 raatlegWAAGADLPLTVLP 349  Auxenochlorella protothecoides

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap