1C1Y,3NY5,2MSE


Conserved Protein Domain Family
RBD_RAF
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cd01816: RBD_RAF 
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Ras-binding domain (RBD) found in RAF family serine/threonine kinases
The RAF family includes three RAF serine/threonine kinases ARAF, BRAF, and RAF1/CRAF. These are encoded by proto-oncogenes, and activate the mitogen-activated protein kinase/extracellular-signal-regulated kinase (MAPK/ERK) cascade downstream of RAS. They share a common structure consisting of an N-terminal regulatory domain and a C-terminal kinase domain. There are three conserved regions (CR1-3) in the regulatory domain, CR1 contains a Ras-binding domain (RBD) and a cysteine-rich domain (CRD), CR2 is a serine/threonine-rich domain, and CR3 encodes the kinase domain required for RAF. The RBD of RAF has a beta-grasp ubiquitin-like fold, a common structure involved in protein-protein interactions.
Links
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Statistics
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PSSM-Id: 340514
Aligned: 36 rows
Threshold Bit Score: 86.4687
Created: 9-Jun-2017
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:RBD_Raf-KRas interaction site [polypeptide binding site]
Evidence:
  • Comment:RBD of ARAF is an effector of a membrane-associated GTPase protein KRAS. KRAS binding to the membrane surface blocks its binding site for effector proteins. Some disease-associated KRAS mutations disrupt this autoinhibition
  • Structure:2MSE; Homo sapiens RBD of ARAF binds GTPase protein KRAS, contacts at 4A.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1         #      # ####              #  ####                                             
1C1Y_B         2 NTIRVFLPNKQRTVVNVRnGMSLHDCLMKALKVRGL----QPECCAVFRLLhehkgKKARLDWNTDAASL--IGEELQVD 75  human
2MSE_D         1 GTVKVYLPNKQRTVVTVRdGMSVYDSLDKALKVRGL----NQDCCVVYRLIk---gRKTVTAWDTAIAPL--DGEELIVE 71  human
CCD78920     187 LRLRAHFPNSQFTLVEIKsGQQIKHALEKKLSHRGL----HLEQLIVYRSR-----TGLPVSWEDDAEQIalIGEELIVD 257 Schistosoma man...
EKC22769     129 GIIKAYLPNEQFSLVKIEpGKILKEGLKKSMSRRFL----SPENCNVYDST-----TKMPISWDIDMANL--AGKDINVC 197 Pacific oyster
GAU93787     226 KIIMAELPNNQKTTVEAIkGKSVQDTLLKKLQHRQL----VPSKCRVFKFDlkdtnKRIAVKWTDDVASI--EGDRIVVE 299 Ramazzottius va...
CDW60339     223 RLLKVHLPFDQHSTVEVKsGVTLREKIGQILTKRNMtytvSPSVCTVTKGPs---vDDEIVSWATDVGTLe-DCEELWVH 298 human whipworm
KPM03320       9 RFLHVHLPNQQRTIIPIQdGQTVRDALARAMKKRQL----TVAMCSVSSCD-----TNEPIAWDSDVADLn-GLTKIEVR 78  Sarcoptes scabiei
XP_001952293  91 RTIRAYLPNKQRTTIEVKhGETLHMALEKRLQHRKI----PLNACVVYRNG-----TDHQIPWDTQTSTI--EYEEIQVK 159 pea aphid
KYM89269     209 SILMIHLPNSQYTTIEVKeGLTLRNAVSKAMERRQL----ILDDCAAYIKQr----MTYFISWDTDISTL--PCKEVFVE 278 Atta colombica
EUB60838     129 CQLRAHLPDNQFTVVEVRpGQTIRQVLEKKLSHRCY----RTEDLSVYSVG-----SGNLVLWDDDAAGValVSGDLVVE 199 Echinococcus gr...

Feature 1          
1C1Y_B        76 FL 77  human
2MSE_D        72 VL 73  human
CCD78920     258 FA 259 Schistosoma mansoni
EKC22769     198 EK 199 Pacific oyster
GAU93787     300 EI 301 Ramazzottius varieornatus
CDW60339     299 IT 300 human whipworm
KPM03320      79 IM 80  Sarcoptes scabiei
XP_001952293 160 MC 161 pea aphid
KYM89269     279 YL 280 Atta colombica
EUB60838     200 FN 201 Echinococcus granulosus

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